Bifunctional lysine-specific demethylase and histidyl-hydroxylase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Cupin superfamily protein, putative

Species:

Leishmania donovani

UniProt:

A0A3S7X447_LEIDO

TriTrypDb:

LdBPK_310250.1 , LdCL_310007600 , LDHU3_31.0310

Length:

507

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	8
GO:0043226	organelle	2	9
GO:0043227	membrane-bounded organelle	3	8
GO:0043229	intracellular organelle	3	9
GO:0043231	intracellular membrane-bounded organelle	4	8
GO:0110165	cellular anatomical entity	1	9
GO:0005730	nucleolus	5	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1

Expansion

Sequence features

A0A3S7X447

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7X447

Function

Biological processes
Term	Name	Level	Count
GO:0006482	protein demethylation	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	2
GO:0008214	protein dealkylation	5	1
GO:0016570	histone modification	5	1
GO:0016577	histone demethylation	4	1
GO:0019538	protein metabolic process	3	1
GO:0034720	histone H3-K4 demethylation	6	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044238	primary metabolic process	2	1
GO:0070076	histone lysine demethylation	5	1
GO:0070544	histone H3-K36 demethylation	6	1
GO:0070988	demethylation	2	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:0032259	methylation	2	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	9
GO:0005488	binding	1	9
GO:0005506	iron ion binding	6	9
GO:0016491	oxidoreductase activity	2	9
GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	3	9
GO:0016706	2-oxoglutarate-dependent dioxygenase activity	4	9
GO:0043167	ion binding	2	9
GO:0043169	cation binding	3	9
GO:0046872	metal ion binding	4	9
GO:0046914	transition metal ion binding	5	9
GO:0051213	dioxygenase activity	3	9
GO:0032451	demethylase activity	2	1
GO:0032452	histone demethylase activity	4	1
GO:0032453	histone H3K4 demethylase activity	5	1
GO:0051864	histone H3K36 demethylase activity	5	1
GO:0140096	catalytic activity, acting on a protein	2	1
GO:0140457	protein demethylase activity	3	1
GO:0008168	methyltransferase activity	4	1
GO:0016740	transferase activity	2	1
GO:0016741	transferase activity, transferring one-carbon groups	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	488	492	PF00656	0.471
CLV_MEL_PAP_1	123	129	PF00089	0.497
CLV_NRD_NRD_1	125	127	PF00675	0.471
CLV_NRD_NRD_1	305	307	PF00675	0.442
CLV_NRD_NRD_1	377	379	PF00675	0.496
CLV_NRD_NRD_1	62	64	PF00675	0.535
CLV_NRD_NRD_1	67	69	PF00675	0.569
CLV_PCSK_KEX2_1	124	126	PF00082	0.459
CLV_PCSK_KEX2_1	14	16	PF00082	0.713
CLV_PCSK_KEX2_1	305	307	PF00082	0.476
CLV_PCSK_PC1ET2_1	124	126	PF00082	0.459
CLV_PCSK_PC1ET2_1	14	16	PF00082	0.727
CLV_PCSK_SKI1_1	305	309	PF00082	0.487
CLV_PCSK_SKI1_1	412	416	PF00082	0.410
DEG_SCF_FBW7_1	235	242	PF00400	0.415
DOC_CKS1_1	182	187	PF01111	0.332
DOC_CKS1_1	236	241	PF01111	0.467
DOC_MAPK_gen_1	122	129	PF00069	0.343
DOC_MAPK_gen_1	14	23	PF00069	0.574
DOC_MAPK_HePTP_8	405	417	PF00069	0.475
DOC_MAPK_MEF2A_6	266	274	PF00069	0.367
DOC_MAPK_MEF2A_6	408	417	PF00069	0.471
DOC_MAPK_NFAT4_5	408	416	PF00069	0.535
DOC_PP1_RVXF_1	123	130	PF00149	0.338
DOC_PP1_RVXF_1	299	305	PF00149	0.497
DOC_PP2B_LxvP_1	251	254	PF13499	0.356
DOC_PP2B_LxvP_1	380	383	PF13499	0.477
DOC_PP2B_LxvP_1	415	418	PF13499	0.519
DOC_PP4_FxxP_1	152	155	PF00568	0.368
DOC_PP4_FxxP_1	236	239	PF00568	0.362
DOC_USP7_MATH_1	185	189	PF00917	0.332
DOC_USP7_MATH_1	224	228	PF00917	0.390
DOC_USP7_MATH_1	37	41	PF00917	0.688
DOC_USP7_UBL2_3	118	122	PF12436	0.554
DOC_USP7_UBL2_3	262	266	PF12436	0.467
DOC_WW_Pin1_4	181	186	PF00397	0.332
DOC_WW_Pin1_4	235	240	PF00397	0.467
LIG_14-3-3_CanoR_1	125	130	PF00244	0.499
LIG_14-3-3_CanoR_1	225	233	PF00244	0.332
LIG_14-3-3_CanoR_1	396	403	PF00244	0.453
LIG_14-3-3_CanoR_1	428	433	PF00244	0.536
LIG_APCC_ABBA_1	191	196	PF00400	0.332
LIG_BRCT_BRCA1_1	148	152	PF00533	0.365
LIG_BRCT_BRCA1_1	187	191	PF00533	0.332
LIG_Clathr_ClatBox_1	202	206	PF01394	0.301
LIG_Clathr_ClatBox_1	483	487	PF01394	0.481
LIG_FHA_1	129	135	PF00498	0.559
LIG_FHA_1	240	246	PF00498	0.406
LIG_FHA_1	265	271	PF00498	0.364
LIG_FHA_1	30	36	PF00498	0.546
LIG_FHA_1	325	331	PF00498	0.602
LIG_FHA_1	354	360	PF00498	0.501
LIG_FHA_1	429	435	PF00498	0.485
LIG_FHA_1	459	465	PF00498	0.553
LIG_FHA_2	486	492	PF00498	0.433
LIG_LIR_Apic_2	149	155	PF02991	0.367
LIG_LIR_Apic_2	181	185	PF02991	0.332
LIG_LIR_Apic_2	234	239	PF02991	0.362
LIG_LIR_Gen_1	281	290	PF02991	0.408
LIG_LIR_Nem_3	170	176	PF02991	0.365
LIG_LIR_Nem_3	208	214	PF02991	0.294
LIG_LIR_Nem_3	281	285	PF02991	0.411
LIG_LIR_Nem_3	60	65	PF02991	0.392
LIG_MYND_1	233	237	PF01753	0.292
LIG_NRBOX	354	360	PF00104	0.431
LIG_PCNA_yPIPBox_3	341	352	PF02747	0.502
LIG_Pex14_1	148	152	PF04695	0.365
LIG_Pex14_2	443	447	PF04695	0.394
LIG_Pex14_2	62	66	PF04695	0.400
LIG_PTB_Apo_2	318	325	PF02174	0.504
LIG_Rb_LxCxE_1	161	181	PF01857	0.428
LIG_Rb_pABgroove_1	208	216	PF01858	0.467
LIG_REV1ctd_RIR_1	59	68	PF16727	0.434
LIG_SH2_CRK	176	180	PF00017	0.391
LIG_SH2_NCK_1	195	199	PF00017	0.332
LIG_SH2_NCK_1	214	218	PF00017	0.332
LIG_SH2_SRC	195	198	PF00017	0.332
LIG_SH2_SRC	214	217	PF00017	0.332
LIG_SH2_STAT5	176	179	PF00017	0.408
LIG_SH2_STAT5	292	295	PF00017	0.475
LIG_SH2_STAT5	65	68	PF00017	0.407
LIG_SH2_STAT5	80	83	PF00017	0.505
LIG_SH3_3	316	322	PF00018	0.472
LIG_SH3_3	85	91	PF00018	0.502
LIG_SUMO_SIM_anti_2	113	118	PF11976	0.437
LIG_SUMO_SIM_anti_2	218	224	PF11976	0.365
LIG_SUMO_SIM_anti_2	354	362	PF11976	0.456
LIG_SUMO_SIM_par_1	354	362	PF11976	0.504
LIG_SUMO_SIM_par_1	482	488	PF11976	0.479
LIG_UBA3_1	202	209	PF00899	0.301
MOD_CDK_SPK_2	235	240	PF00069	0.390
MOD_CK1_1	128	134	PF00069	0.565
MOD_CK1_1	181	187	PF00069	0.332
MOD_CK1_1	398	404	PF00069	0.502
MOD_CK1_1	40	46	PF00069	0.622
MOD_CK1_1	74	80	PF00069	0.493
MOD_CK2_1	54	60	PF00069	0.391
MOD_GlcNHglycan	10	13	PF01048	0.739
MOD_GlcNHglycan	162	165	PF01048	0.325
MOD_GlcNHglycan	274	277	PF01048	0.309
MOD_GlcNHglycan	387	390	PF01048	0.540
MOD_GlcNHglycan	464	467	PF01048	0.532
MOD_GlcNHglycan	499	502	PF01048	0.716
MOD_GlcNHglycan	76	79	PF01048	0.518
MOD_GlcNHglycan	82	85	PF01048	0.482
MOD_GSK3_1	17	24	PF00069	0.700
MOD_GSK3_1	181	188	PF00069	0.307
MOD_GSK3_1	235	242	PF00069	0.399
MOD_GSK3_1	260	267	PF00069	0.318
MOD_GSK3_1	353	360	PF00069	0.437
MOD_GSK3_1	381	388	PF00069	0.508
MOD_GSK3_1	391	398	PF00069	0.460
MOD_GSK3_1	423	430	PF00069	0.370
MOD_GSK3_1	450	457	PF00069	0.425
MOD_GSK3_1	458	465	PF00069	0.438
MOD_N-GLC_1	428	433	PF02516	0.491
MOD_N-GLC_1	92	97	PF02516	0.473
MOD_NEK2_1	158	163	PF00069	0.487
MOD_NEK2_1	21	26	PF00069	0.649
MOD_NEK2_1	270	275	PF00069	0.332
MOD_NEK2_1	324	329	PF00069	0.593
MOD_NEK2_1	454	459	PF00069	0.446
MOD_NEK2_1	485	490	PF00069	0.414
MOD_NEK2_1	53	58	PF00069	0.368
MOD_NEK2_1	71	76	PF00069	0.388
MOD_NEK2_2	146	151	PF00069	0.338
MOD_NEK2_2	254	259	PF00069	0.400
MOD_NEK2_2	261	266	PF00069	0.365
MOD_NEK2_2	373	378	PF00069	0.473
MOD_PIKK_1	185	191	PF00454	0.332
MOD_PIKK_1	40	46	PF00454	0.651
MOD_PKA_1	125	131	PF00069	0.502
MOD_PKA_1	8	14	PF00069	0.728
MOD_PKA_2	125	131	PF00069	0.454
MOD_PKA_2	224	230	PF00069	0.307
MOD_PKA_2	254	260	PF00069	0.365
MOD_PKA_2	324	330	PF00069	0.529
MOD_PKA_2	395	401	PF00069	0.451
MOD_PKA_2	427	433	PF00069	0.537
MOD_PKB_1	15	23	PF00069	0.711
MOD_PKB_1	402	410	PF00069	0.455
MOD_Plk_1	17	23	PF00069	0.713
MOD_Plk_1	353	359	PF00069	0.453
MOD_Plk_1	428	434	PF00069	0.494
MOD_Plk_4	178	184	PF00069	0.310
MOD_Plk_4	353	359	PF00069	0.462
MOD_Plk_4	450	456	PF00069	0.366
MOD_ProDKin_1	181	187	PF00069	0.332
MOD_ProDKin_1	235	241	PF00069	0.467
MOD_SUMO_for_1	28	31	PF00179	0.697
MOD_SUMO_rev_2	115	123	PF00179	0.461
TRG_DiLeu_BaEn_1	354	359	PF01217	0.450
TRG_DiLeu_BaEn_1	480	485	PF01217	0.491
TRG_DiLeu_BaEn_1	67	72	PF01217	0.464
TRG_DiLeu_BaLyEn_6	401	406	PF01217	0.492
TRG_DiLeu_BaLyEn_6	409	414	PF01217	0.426
TRG_ENDOCYTIC_2	176	179	PF00928	0.386
TRG_ENDOCYTIC_2	195	198	PF00928	0.269
TRG_ER_diArg_1	125	127	PF00400	0.471
TRG_ER_diArg_1	304	306	PF00400	0.451
TRG_ER_diArg_1	402	405	PF00400	0.428
TRG_NLS_MonoExtN_4	121	128	PF00514	0.507

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PDT4	Leptomonas seymouri	70%	99%
A0A1X0NJI1	Trypanosomatidae	53%	100%
A3KP59	Danio rerio	28%	93%
A4HIZ9	Leishmania braziliensis	81%	100%
A4I6B6	Leishmania infantum	99%	100%
A5PK74	Bos taurus	31%	76%
A8QFQ3	Brugia malayi	25%	71%
B0WMG3	Culex quinquefasciatus	30%	78%
B3NU20	Drosophila erecta	28%	77%
B4GUZ2	Drosophila persimilis	30%	74%
B4I100	Drosophila sechellia	30%	77%
B4JMQ2	Drosophila grimshawi	33%	70%
B4NP88	Drosophila willistoni	30%	66%
B4Q068	Drosophila yakuba	28%	74%
C3XRY1	Branchiostoma floridae	28%	84%
D3ZU57	Rattus norvegicus	31%	85%
E9B1H4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
O01658	Caenorhabditis elegans	25%	68%
Q16W06	Aedes aegypti	28%	80%
Q4Q6P0	Leishmania major	93%	100%
Q54K96	Dictyostelium discoideum	30%	99%
Q5ZMM1	Gallus gallus	29%	84%
Q7K4H4	Drosophila melanogaster	29%	78%
Q9H6W3	Homo sapiens	31%	79%
Q9JJF3	Mus musculus	31%	84%
V5AYK7	Trypanosoma cruzi	54%	100%