GRAM domain containing protein, putative

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

GRAM domain containing protein, putative

Species:

Leishmania donovani

UniProt:

A0A3S7X3S1_LEIDO

TriTrypDb:

LdBPK_302720.1 , LdCL_300032800 , LDHU3_30.3640

Length:

730

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0005789	endoplasmic reticulum membrane	4	1
GO:0005886	plasma membrane	3	1
GO:0016020	membrane	2	1
GO:0031090	organelle membrane	3	1
GO:0044232	organelle membrane contact site	2	1
GO:0110165	cellular anatomical entity	1	1
GO:0140268	endoplasmic reticulum-plasma membrane contact site	3	1

Expansion

Sequence features

A0A3S7X3S1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7X3S1

PDB structure(s): 4tyz_A , 4tyz_B

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	1
GO:0006869	lipid transport	5	1
GO:0009987	cellular process	1	1
GO:0015850	organic hydroxy compound transport	5	1
GO:0015918	sterol transport	6	1
GO:0032365	intracellular lipid transport	4	1
GO:0032366	intracellular sterol transport	5	1
GO:0046907	intracellular transport	3	1
GO:0051179	localization	1	1
GO:0051234	establishment of localization	2	1
GO:0051641	cellular localization	2	1
GO:0051649	establishment of localization in cell	3	1
GO:0071702	organic substance transport	4	1

Molecular functions
Term	Name	Level	Count
GO:0005215	transporter activity	1	1
GO:0005319	lipid transporter activity	2	1
GO:0005488	binding	1	1
GO:0005496	steroid binding	3	1
GO:0008289	lipid binding	2	1
GO:0015248	sterol transporter activity	3	1
GO:0032934	sterol binding	4	1
GO:0097159	organic cyclic compound binding	2	1
GO:0120013	lipid transfer activity	3	1
GO:0120015	sterol transfer activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	195	199	PF00656	0.616
CLV_C14_Caspase3-7	473	477	PF00656	0.516
CLV_C14_Caspase3-7	520	524	PF00656	0.564
CLV_C14_Caspase3-7	637	641	PF00656	0.438
CLV_NRD_NRD_1	186	188	PF00675	0.794
CLV_NRD_NRD_1	221	223	PF00675	0.789
CLV_NRD_NRD_1	434	436	PF00675	0.517
CLV_NRD_NRD_1	534	536	PF00675	0.556
CLV_NRD_NRD_1	626	628	PF00675	0.356
CLV_PCSK_FUR_1	532	536	PF00082	0.555
CLV_PCSK_KEX2_1	185	187	PF00082	0.786
CLV_PCSK_KEX2_1	245	247	PF00082	0.635
CLV_PCSK_KEX2_1	434	436	PF00082	0.517
CLV_PCSK_KEX2_1	534	536	PF00082	0.556
CLV_PCSK_PC1ET2_1	245	247	PF00082	0.635
CLV_PCSK_SKI1_1	134	138	PF00082	0.547
CLV_PCSK_SKI1_1	224	228	PF00082	0.763
CLV_PCSK_SKI1_1	292	296	PF00082	0.739
CLV_PCSK_SKI1_1	544	548	PF00082	0.765
CLV_PCSK_SKI1_1	689	693	PF00082	0.341
DOC_CKS1_1	658	663	PF01111	0.349
DOC_CYCLIN_yCln2_LP_2	594	600	PF00134	0.677
DOC_PP1_RVXF_1	542	549	PF00149	0.688
DOC_PP2B_LxvP_1	594	597	PF13499	0.671
DOC_PP4_FxxP_1	401	404	PF00568	0.451
DOC_PP4_FxxP_1	559	562	PF00568	0.720
DOC_PP4_FxxP_1	658	661	PF00568	0.373
DOC_USP7_MATH_1	153	157	PF00917	0.734
DOC_USP7_MATH_1	176	180	PF00917	0.804
DOC_USP7_MATH_1	232	236	PF00917	0.752
DOC_USP7_MATH_1	273	277	PF00917	0.693
DOC_USP7_MATH_1	286	290	PF00917	0.761
DOC_USP7_MATH_1	34	38	PF00917	0.634
DOC_USP7_MATH_1	425	429	PF00917	0.451
DOC_USP7_MATH_1	528	532	PF00917	0.625
DOC_USP7_MATH_1	56	60	PF00917	0.539
DOC_USP7_MATH_1	722	726	PF00917	0.438
DOC_USP7_UBL2_3	219	223	PF12436	0.696
DOC_USP7_UBL2_3	683	687	PF12436	0.438
DOC_WW_Pin1_4	238	243	PF00397	0.768
DOC_WW_Pin1_4	302	307	PF00397	0.664
DOC_WW_Pin1_4	4	9	PF00397	0.623
DOC_WW_Pin1_4	400	405	PF00397	0.470
DOC_WW_Pin1_4	657	662	PF00397	0.349
LIG_14-3-3_CanoR_1	27	36	PF00244	0.622
LIG_14-3-3_CanoR_1	434	440	PF00244	0.462
LIG_14-3-3_CanoR_1	483	489	PF00244	0.562
LIG_14-3-3_CanoR_1	627	631	PF00244	0.324
LIG_Actin_WH2_2	26	43	PF00022	0.595
LIG_AP2alpha_2	399	401	PF02296	0.548
LIG_BIR_II_1	1	5	PF00653	0.712
LIG_BIR_III_4	328	332	PF00653	0.469
LIG_BIR_III_4	610	614	PF00653	0.562
LIG_BRCT_BRCA1_1	317	321	PF00533	0.394
LIG_eIF4E_1	437	443	PF01652	0.414
LIG_FHA_1	173	179	PF00498	0.632
LIG_FHA_1	321	327	PF00498	0.532
LIG_FHA_1	354	360	PF00498	0.392
LIG_FHA_1	450	456	PF00498	0.484
LIG_FHA_1	483	489	PF00498	0.492
LIG_FHA_1	49	55	PF00498	0.665
LIG_FHA_1	492	498	PF00498	0.496
LIG_FHA_1	514	520	PF00498	0.544
LIG_FHA_1	613	619	PF00498	0.560
LIG_FHA_1	705	711	PF00498	0.435
LIG_FHA_2	226	232	PF00498	0.730
LIG_FHA_2	303	309	PF00498	0.599
LIG_FHA_2	323	329	PF00498	0.516
LIG_FHA_2	347	353	PF00498	0.515
LIG_FHA_2	380	386	PF00498	0.572
LIG_FHA_2	471	477	PF00498	0.505
LIG_FHA_2	5	11	PF00498	0.604
LIG_FHA_2	635	641	PF00498	0.438
LIG_LIR_Apic_2	347	353	PF02991	0.600
LIG_LIR_Apic_2	399	404	PF02991	0.496
LIG_LIR_Apic_2	556	562	PF02991	0.717
LIG_LIR_Gen_1	316	326	PF02991	0.464
LIG_LIR_Gen_1	355	364	PF02991	0.411
LIG_LIR_Gen_1	438	447	PF02991	0.449
LIG_LIR_Gen_1	480	489	PF02991	0.552
LIG_LIR_Gen_1	516	526	PF02991	0.535
LIG_LIR_Gen_1	660	671	PF02991	0.367
LIG_LIR_Gen_1	676	686	PF02991	0.247
LIG_LIR_Gen_1	717	728	PF02991	0.357
LIG_LIR_Nem_3	247	253	PF02991	0.731
LIG_LIR_Nem_3	316	322	PF02991	0.442
LIG_LIR_Nem_3	355	361	PF02991	0.402
LIG_LIR_Nem_3	366	372	PF02991	0.337
LIG_LIR_Nem_3	438	443	PF02991	0.412
LIG_LIR_Nem_3	480	484	PF02991	0.543
LIG_LIR_Nem_3	516	521	PF02991	0.528
LIG_LIR_Nem_3	629	633	PF02991	0.337
LIG_LIR_Nem_3	660	666	PF02991	0.367
LIG_LIR_Nem_3	676	682	PF02991	0.276
LIG_LIR_Nem_3	717	723	PF02991	0.438
LIG_PCNA_yPIPBox_3	351	363	PF02747	0.566
LIG_Pex14_2	104	108	PF04695	0.553
LIG_Pex14_2	626	630	PF04695	0.357
LIG_Pex14_2	642	646	PF04695	0.357
LIG_Pex14_2	709	713	PF04695	0.357
LIG_REV1ctd_RIR_1	135	143	PF16727	0.664
LIG_SH2_CRK	543	547	PF00017	0.546
LIG_SH2_CRK	720	724	PF00017	0.430
LIG_SH2_NCK_1	116	120	PF00017	0.578
LIG_SH2_NCK_1	440	444	PF00017	0.511
LIG_SH2_PTP2	358	361	PF00017	0.399
LIG_SH2_SRC	679	682	PF00017	0.438
LIG_SH2_STAP1	208	212	PF00017	0.725
LIG_SH2_STAP1	437	441	PF00017	0.401
LIG_SH2_STAP1	720	724	PF00017	0.345
LIG_SH2_STAT5	319	322	PF00017	0.455
LIG_SH2_STAT5	358	361	PF00017	0.375
LIG_SH2_STAT5	369	372	PF00017	0.362
LIG_SH2_STAT5	375	378	PF00017	0.421
LIG_SH2_STAT5	437	440	PF00017	0.403
LIG_SH2_STAT5	656	659	PF00017	0.391
LIG_SH3_2	283	288	PF14604	0.817
LIG_SH3_3	280	286	PF00018	0.809
LIG_SH3_3	516	522	PF00018	0.491
LIG_SUMO_SIM_anti_2	30	37	PF11976	0.608
LIG_SUMO_SIM_par_1	470	476	PF11976	0.511
LIG_TRAF2_1	549	552	PF00917	0.723
LIG_TYR_ITIM	677	682	PF00017	0.491
LIG_TYR_ITSM	436	443	PF00017	0.447
LIG_WRC_WIRS_1	105	110	PF05994	0.566
LIG_WRC_WIRS_1	345	350	PF05994	0.545
LIG_WRC_WIRS_1	505	510	PF05994	0.520
LIG_WRC_WIRS_1	639	644	PF05994	0.391
LIG_WW_3	445	449	PF00397	0.590
MOD_CDK_SPxxK_3	238	245	PF00069	0.666
MOD_CK1_1	141	147	PF00069	0.561
MOD_CK1_1	256	262	PF00069	0.766
MOD_CK1_1	290	296	PF00069	0.748
MOD_CK1_1	315	321	PF00069	0.517
MOD_CK1_1	379	385	PF00069	0.605
MOD_CK1_1	59	65	PF00069	0.556
MOD_CK2_1	225	231	PF00069	0.715
MOD_CK2_1	321	327	PF00069	0.522
MOD_CK2_1	346	352	PF00069	0.520
MOD_CK2_1	4	10	PF00069	0.621
MOD_CK2_1	521	527	PF00069	0.534
MOD_CK2_1	546	552	PF00069	0.564
MOD_CK2_1	71	77	PF00069	0.513
MOD_Cter_Amidation	183	186	PF01082	0.756
MOD_Cter_Amidation	220	223	PF01082	0.677
MOD_GlcNHglycan	1	4	PF01048	0.678
MOD_GlcNHglycan	210	213	PF01048	0.807
MOD_GlcNHglycan	22	25	PF01048	0.555
MOD_GlcNHglycan	233	237	PF01048	0.801
MOD_GlcNHglycan	261	264	PF01048	0.728
MOD_GlcNHglycan	275	278	PF01048	0.703
MOD_GlcNHglycan	378	381	PF01048	0.563
MOD_GlcNHglycan	510	513	PF01048	0.569
MOD_GlcNHglycan	648	652	PF01048	0.429
MOD_GlcNHglycan	720	723	PF01048	0.414
MOD_GSK3_1	103	110	PF00069	0.552
MOD_GSK3_1	139	146	PF00069	0.564
MOD_GSK3_1	147	154	PF00069	0.615
MOD_GSK3_1	172	179	PF00069	0.739
MOD_GSK3_1	234	241	PF00069	0.744
MOD_GSK3_1	255	262	PF00069	0.734
MOD_GSK3_1	273	280	PF00069	0.499
MOD_GSK3_1	286	293	PF00069	0.736
MOD_GSK3_1	340	347	PF00069	0.501
MOD_GSK3_1	45	52	PF00069	0.664
MOD_GSK3_1	487	494	PF00069	0.490
MOD_GSK3_1	504	511	PF00069	0.511
MOD_GSK3_1	542	549	PF00069	0.557
MOD_GSK3_1	634	641	PF00069	0.438
MOD_GSK3_1	67	74	PF00069	0.532
MOD_GSK3_1	704	711	PF00069	0.336
MOD_GSK3_1	718	725	PF00069	0.351
MOD_LATS_1	25	31	PF00433	0.571
MOD_N-GLC_1	20	25	PF02516	0.564
MOD_N-GLC_1	4	9	PF02516	0.524
MOD_N-GLC_1	70	75	PF02516	0.504
MOD_N-GLC_1	704	709	PF02516	0.438
MOD_NEK2_1	104	109	PF00069	0.557
MOD_NEK2_1	138	143	PF00069	0.570
MOD_NEK2_1	320	325	PF00069	0.523
MOD_NEK2_1	506	511	PF00069	0.526
MOD_NEK2_1	513	518	PF00069	0.541
MOD_NEK2_1	553	558	PF00069	0.698
MOD_NEK2_1	609	614	PF00069	0.563
MOD_NEK2_1	626	631	PF00069	0.288
MOD_NEK2_1	638	643	PF00069	0.363
MOD_NEK2_1	666	671	PF00069	0.464
MOD_NEK2_1	704	709	PF00069	0.336
MOD_NEK2_2	177	182	PF00069	0.607
MOD_OFUCOSY	69	75	PF10250	0.594
MOD_PIKK_1	107	113	PF00454	0.553
MOD_PIKK_1	244	250	PF00454	0.795
MOD_PIKK_1	253	259	PF00454	0.571
MOD_PIKK_1	34	40	PF00454	0.640
MOD_PIKK_1	379	385	PF00454	0.557
MOD_PIKK_1	496	502	PF00454	0.556
MOD_PIKK_1	722	728	PF00454	0.438
MOD_PK_1	139	145	PF00069	0.631
MOD_PK_1	687	693	PF00069	0.491
MOD_PKA_1	224	230	PF00069	0.771
MOD_PKA_2	138	144	PF00069	0.581
MOD_PKA_2	259	265	PF00069	0.641
MOD_PKA_2	482	488	PF00069	0.560
MOD_PKA_2	626	632	PF00069	0.351
MOD_PKB_1	168	176	PF00069	0.599
MOD_Plk_1	139	145	PF00069	0.697
MOD_Plk_1	263	269	PF00069	0.705
MOD_Plk_1	414	420	PF00069	0.572
MOD_Plk_1	59	65	PF00069	0.572
MOD_Plk_1	704	710	PF00069	0.438
MOD_Plk_4	104	110	PF00069	0.522
MOD_Plk_4	315	321	PF00069	0.470
MOD_Plk_4	353	359	PF00069	0.433
MOD_Plk_4	435	441	PF00069	0.421
MOD_Plk_4	470	476	PF00069	0.502
MOD_Plk_4	542	548	PF00069	0.601
MOD_Plk_4	634	640	PF00069	0.399
MOD_Plk_4	704	710	PF00069	0.357
MOD_ProDKin_1	238	244	PF00069	0.769
MOD_ProDKin_1	302	308	PF00069	0.658
MOD_ProDKin_1	4	10	PF00069	0.617
MOD_ProDKin_1	400	406	PF00069	0.470
MOD_ProDKin_1	657	663	PF00069	0.349
MOD_SUMO_rev_2	676	685	PF00179	0.438
MOD_SUMO_rev_2	695	702	PF00179	0.438
TRG_DiLeu_BaLyEn_6	357	362	PF01217	0.462
TRG_DiLeu_BaLyEn_6	96	101	PF01217	0.566
TRG_ENDOCYTIC_2	116	119	PF00928	0.487
TRG_ENDOCYTIC_2	319	322	PF00928	0.455
TRG_ENDOCYTIC_2	358	361	PF00928	0.391
TRG_ENDOCYTIC_2	440	443	PF00928	0.407
TRG_ENDOCYTIC_2	489	492	PF00928	0.561
TRG_ENDOCYTIC_2	543	546	PF00928	0.545
TRG_ENDOCYTIC_2	656	659	PF00928	0.438
TRG_ENDOCYTIC_2	679	682	PF00928	0.438
TRG_ENDOCYTIC_2	720	723	PF00928	0.345
TRG_ER_diArg_1	185	187	PF00400	0.769
TRG_ER_diArg_1	434	436	PF00400	0.517
TRG_ER_diArg_1	532	535	PF00400	0.559
TRG_NLS_MonoCore_2	221	226	PF00514	0.708
TRG_NLS_MonoExtC_3	218	223	PF00514	0.723
TRG_NLS_MonoExtN_4	218	223	PF00514	0.679
TRG_NLS_MonoExtN_4	532	539	PF00514	0.562
TRG_Pf-PMV_PEXEL_1	27	31	PF00026	0.622

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I443	Leptomonas seymouri	57%	100%
A0A3R7L2R2	Trypanosoma rangeli	35%	100%
A4HIK4	Leishmania braziliensis	69%	97%
A4I5U9	Leishmania infantum	100%	100%
E9B144	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
Q4Q722	Leishmania major	90%	100%
V5B7B2	Trypanosoma cruzi	37%	100%