tRNA pseudouridine synthase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

pseudouridine synthase A-like protein, putative

Species:

Leishmania donovani

UniProt:

A0A3S7X3E8_LEIDO

TriTrypDb:

LdBPK_301570.1 , LdCL_300021000 , LDHU3_30.2050

Length:

499

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005737	cytoplasm	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A0A3S7X3E8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7X3E8

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	11
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006399	tRNA metabolic process	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008033	tRNA processing	8	12
GO:0008152	metabolic process	1	12
GO:0009451	RNA modification	5	11
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0034470	ncRNA processing	7	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	11
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006400	tRNA modification	6	1
GO:0016071	mRNA metabolic process	6	1
GO:0016556	mRNA modification	6	1
GO:0031119	tRNA pseudouridine synthesis	7	1
GO:1990481	mRNA pseudouridine synthesis	7	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0009982	pseudouridine synthase activity	4	12
GO:0016829	lyase activity	2	7
GO:0016853	isomerase activity	2	12
GO:0016866	intramolecular transferase activity	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0106029	tRNA pseudouridine synthase activity	5	9
GO:0140098	catalytic activity, acting on RNA	3	9
GO:0140101	catalytic activity, acting on a tRNA	4	9
GO:0140640	catalytic activity, acting on a nucleic acid	2	9
GO:1901363	heterocyclic compound binding	2	12
GO:0004730	pseudouridylate synthase activity	5	1
GO:0016835	carbon-oxygen lyase activity	3	1
GO:0016836	hydro-lyase activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	284	286	PF00675	0.261
CLV_NRD_NRD_1	355	357	PF00675	0.641
CLV_NRD_NRD_1	382	384	PF00675	0.508
CLV_NRD_NRD_1	405	407	PF00675	0.468
CLV_NRD_NRD_1	59	61	PF00675	0.338
CLV_NRD_NRD_1	95	97	PF00675	0.445
CLV_PCSK_KEX2_1	382	384	PF00082	0.650
CLV_PCSK_KEX2_1	407	409	PF00082	0.606
CLV_PCSK_KEX2_1	474	476	PF00082	0.392
CLV_PCSK_KEX2_1	95	97	PF00082	0.415
CLV_PCSK_PC1ET2_1	407	409	PF00082	0.593
CLV_PCSK_PC1ET2_1	474	476	PF00082	0.392
CLV_PCSK_SKI1_1	224	228	PF00082	0.326
CLV_PCSK_SKI1_1	408	412	PF00082	0.552
CLV_PCSK_SKI1_1	50	54	PF00082	0.419
CLV_PCSK_SKI1_1	61	65	PF00082	0.247
DEG_APCC_DBOX_1	407	415	PF00400	0.591
DEG_SPOP_SBC_1	2	6	PF00917	0.566
DEG_SPOP_SBC_1	423	427	PF00917	0.579
DOC_CYCLIN_RxL_1	182	193	PF00134	0.256
DOC_CYCLIN_RxL_1	92	103	PF00134	0.340
DOC_MAPK_gen_1	60	66	PF00069	0.286
DOC_MAPK_MEF2A_6	257	264	PF00069	0.255
DOC_PP1_RVXF_1	370	377	PF00149	0.423
DOC_PP2B_LxvP_1	270	273	PF13499	0.315
DOC_USP7_MATH_1	129	133	PF00917	0.421
DOC_USP7_MATH_1	2	6	PF00917	0.585
DOC_USP7_MATH_1	339	343	PF00917	0.494
DOC_WW_Pin1_4	292	297	PF00397	0.274
LIG_14-3-3_CanoR_1	110	118	PF00244	0.329
LIG_14-3-3_CanoR_1	130	139	PF00244	0.230
LIG_14-3-3_CanoR_1	173	181	PF00244	0.281
LIG_14-3-3_CanoR_1	224	230	PF00244	0.346
LIG_14-3-3_CanoR_1	247	252	PF00244	0.328
LIG_14-3-3_CanoR_1	333	340	PF00244	0.385
LIG_Actin_WH2_2	82	100	PF00022	0.398
LIG_BIR_II_1	1	5	PF00653	0.723
LIG_BIR_III_2	136	140	PF00653	0.553
LIG_BRCT_BRCA1_1	341	345	PF00533	0.408
LIG_deltaCOP1_diTrp_1	385	394	PF00928	0.416
LIG_EH1_1	253	261	PF00400	0.243
LIG_FHA_1	155	161	PF00498	0.287
LIG_FHA_1	286	292	PF00498	0.309
LIG_FHA_1	293	299	PF00498	0.299
LIG_FHA_1	326	332	PF00498	0.375
LIG_FHA_1	33	39	PF00498	0.492
LIG_FHA_2	276	282	PF00498	0.383
LIG_FHA_2	363	369	PF00498	0.556
LIG_FHA_2	388	394	PF00498	0.458
LIG_FHA_2	4	10	PF00498	0.548
LIG_FHA_2	426	432	PF00498	0.728
LIG_FHA_2	460	466	PF00498	0.404
LIG_Integrin_RGD_1	282	284	PF01839	0.180
LIG_LIR_Apic_2	393	397	PF02991	0.435
LIG_LIR_Gen_1	145	154	PF02991	0.324
LIG_LIR_Gen_1	171	181	PF02991	0.312
LIG_LIR_Gen_1	242	251	PF02991	0.258
LIG_LIR_Gen_1	301	311	PF02991	0.446
LIG_LIR_Nem_3	171	177	PF02991	0.301
LIG_LIR_Nem_3	180	186	PF02991	0.250
LIG_LIR_Nem_3	205	211	PF02991	0.333
LIG_LIR_Nem_3	242	246	PF02991	0.240
LIG_LIR_Nem_3	250	256	PF02991	0.247
LIG_LIR_Nem_3	301	307	PF02991	0.255
LIG_LIR_Nem_3	342	348	PF02991	0.419
LIG_MYND_1	139	143	PF01753	0.458
LIG_Pex14_1	300	304	PF04695	0.240
LIG_Pex14_2	170	174	PF04695	0.288
LIG_REV1ctd_RIR_1	183	192	PF16727	0.330
LIG_SH2_CRK	253	257	PF00017	0.240
LIG_SH2_PTP2	451	454	PF00017	0.414
LIG_SH2_SRC	243	246	PF00017	0.333
LIG_SH2_STAP1	463	467	PF00017	0.451
LIG_SH2_STAT5	146	149	PF00017	0.287
LIG_SH2_STAT5	184	187	PF00017	0.417
LIG_SH2_STAT5	243	246	PF00017	0.255
LIG_SH2_STAT5	348	351	PF00017	0.465
LIG_SH2_STAT5	413	416	PF00017	0.604
LIG_SH2_STAT5	451	454	PF00017	0.374
LIG_SH3_3	136	142	PF00018	0.463
LIG_SH3_3	284	290	PF00018	0.240
LIG_SH3_3	442	448	PF00018	0.690
LIG_SH3_5	142	146	PF00018	0.346
LIG_SUMO_SIM_par_1	295	301	PF11976	0.194
LIG_TRAF2_1	194	197	PF00917	0.430
LIG_TRAF2_1	429	432	PF00917	0.596
LIG_TRAF2_1	462	465	PF00917	0.475
LIG_TYR_ITIM	241	246	PF00017	0.255
LIG_TYR_ITIM	449	454	PF00017	0.399
LIG_UBA3_1	37	45	PF00899	0.455
MOD_CK1_1	19	25	PF00069	0.652
MOD_CK1_1	3	9	PF00069	0.698
MOD_CK1_1	363	369	PF00069	0.624
MOD_CK1_1	425	431	PF00069	0.730
MOD_CK2_1	239	245	PF00069	0.244
MOD_CK2_1	275	281	PF00069	0.383
MOD_CK2_1	362	368	PF00069	0.590
MOD_CK2_1	425	431	PF00069	0.643
MOD_CK2_1	459	465	PF00069	0.415
MOD_Cter_Amidation	471	474	PF01082	0.530
MOD_GlcNHglycan	118	121	PF01048	0.317
MOD_GlcNHglycan	19	22	PF01048	0.479
MOD_GlcNHglycan	335	338	PF01048	0.462
MOD_GlcNHglycan	364	368	PF01048	0.626
MOD_GlcNHglycan	442	445	PF01048	0.612
MOD_GlcNHglycan	469	473	PF01048	0.482
MOD_GlcNHglycan	8	13	PF01048	0.623
MOD_GSK3_1	125	132	PF00069	0.318
MOD_GSK3_1	13	20	PF00069	0.728
MOD_GSK3_1	271	278	PF00069	0.383
MOD_GSK3_1	356	363	PF00069	0.514
MOD_GSK3_1	392	399	PF00069	0.468
MOD_GSK3_1	422	429	PF00069	0.626
MOD_N-GLC_1	217	222	PF02516	0.277
MOD_N-GLC_2	210	212	PF02516	0.240
MOD_NEK2_1	1	6	PF00069	0.738
MOD_NEK2_1	125	130	PF00069	0.337
MOD_NEK2_1	202	207	PF00069	0.333
MOD_NEK2_1	222	227	PF00069	0.315
MOD_NEK2_1	40	45	PF00069	0.599
MOD_NEK2_1	424	429	PF00069	0.578
MOD_PIKK_1	339	345	PF00454	0.386
MOD_PIKK_1	432	438	PF00454	0.581
MOD_PKA_1	285	291	PF00069	0.243
MOD_PKA_1	356	362	PF00069	0.502
MOD_PKA_2	129	135	PF00069	0.321
MOD_PKA_2	172	178	PF00069	0.287
MOD_PKA_2	332	338	PF00069	0.384
MOD_PKA_2	456	462	PF00069	0.391
MOD_Plk_1	459	465	PF00069	0.377
MOD_Plk_1	8	14	PF00069	0.566
MOD_Plk_1	82	88	PF00069	0.455
MOD_Plk_2-3	239	245	PF00069	0.240
MOD_Plk_4	145	151	PF00069	0.364
MOD_Plk_4	247	253	PF00069	0.273
MOD_Plk_4	271	277	PF00069	0.363
MOD_ProDKin_1	292	298	PF00069	0.274
MOD_SUMO_rev_2	281	288	PF00179	0.244
MOD_SUMO_rev_2	425	435	PF00179	0.618
MOD_SUMO_rev_2	479	486	PF00179	0.473
TRG_DiLeu_BaEn_1	261	266	PF01217	0.255
TRG_DiLeu_BaEn_4	465	471	PF01217	0.392
TRG_DiLeu_BaLyEn_6	450	455	PF01217	0.518
TRG_DiLeu_BaLyEn_6	95	100	PF01217	0.318
TRG_ENDOCYTIC_2	146	149	PF00928	0.303
TRG_ENDOCYTIC_2	183	186	PF00928	0.402
TRG_ENDOCYTIC_2	243	246	PF00928	0.247
TRG_ENDOCYTIC_2	253	256	PF00928	0.227
TRG_ENDOCYTIC_2	451	454	PF00928	0.355
TRG_ER_diArg_1	381	383	PF00400	0.485
TRG_ER_diArg_1	475	478	PF00400	0.383
TRG_ER_diArg_1	94	96	PF00400	0.328
TRG_NLS_MonoExtC_3	43	49	PF00514	0.464
TRG_NLS_MonoExtC_3	472	477	PF00514	0.394
TRG_NLS_MonoExtN_4	473	478	PF00514	0.391
TRG_Pf-PMV_PEXEL_1	188	193	PF00026	0.269
TRG_Pf-PMV_PEXEL_1	257	261	PF00026	0.315
TRG_Pf-PMV_PEXEL_1	98	102	PF00026	0.404

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IA61	Leptomonas seymouri	67%	97%
A0A0S4JB03	Bodo saltans	51%	100%
A0A1X0P1U9	Trypanosomatidae	58%	100%
A0A422P3K1	Trypanosoma rangeli	59%	100%
A4HIA0	Leishmania braziliensis	82%	100%
A4I5I4	Leishmania infantum	100%	100%
C9ZQW8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	54%	100%
E9B0S9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
P31115	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	38%	100%
Q09524	Caenorhabditis elegans	35%	100%
Q4Q7E9	Leishmania major	95%	100%
Q9BZE2	Homo sapiens	38%	100%
Q9JI38	Mus musculus	36%	100%
V5DQN1	Trypanosoma cruzi	61%	95%