Non-specific serine/threonine protein kinase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Rio2, N-terminal/RIO1 family, putative

Species:

Leishmania donovani

UniProt:

A0A3S7X3A2_LEIDO

TriTrypDb:

LdBPK_301420.1 , LdCL_300019400 , LDHU3_30.1800

Length:

486

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005829	cytosol	2	1
GO:0030684	preribosome	3	1
GO:0030688	preribosome, small subunit precursor	4	1
GO:0032991	protein-containing complex	1	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1
GO:1990904	ribonucleoprotein complex	2	1

Expansion

Sequence features

A0A3S7X3A2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7X3A2

Function

Biological processes
Term	Name	Level	Count
GO:0006468	protein phosphorylation	5	11
GO:0006793	phosphorus metabolic process	3	11
GO:0006796	phosphate-containing compound metabolic process	4	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0016310	phosphorylation	5	11
GO:0019538	protein metabolic process	3	11
GO:0036211	protein modification process	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0071704	organic substance metabolic process	2	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006364	rRNA processing	8	1
GO:0006396	RNA processing	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0016070	RNA metabolic process	5	1
GO:0016072	rRNA metabolic process	7	1
GO:0030490	maturation of SSU-rRNA	9	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0046483	heterocycle metabolic process	3	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003824	catalytic activity	1	11
GO:0004672	protein kinase activity	3	11
GO:0004674	protein serine/threonine kinase activity	4	11
GO:0005488	binding	1	11
GO:0005524	ATP binding	5	11
GO:0016301	kinase activity	4	11
GO:0016740	transferase activity	2	11
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	11
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	11
GO:0017076	purine nucleotide binding	4	11
GO:0030554	adenyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:0140096	catalytic activity, acting on a protein	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:0106310	protein serine kinase activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	203	207	PF00656	0.485
CLV_NRD_NRD_1	212	214	PF00675	0.225
CLV_NRD_NRD_1	277	279	PF00675	0.212
CLV_NRD_NRD_1	294	296	PF00675	0.390
CLV_NRD_NRD_1	310	312	PF00675	0.492
CLV_NRD_NRD_1	412	414	PF00675	0.568
CLV_NRD_NRD_1	443	445	PF00675	0.472
CLV_NRD_NRD_1	448	450	PF00675	0.480
CLV_NRD_NRD_1	49	51	PF00675	0.212
CLV_PCSK_FUR_1	410	414	PF00082	0.640
CLV_PCSK_KEX2_1	212	214	PF00082	0.215
CLV_PCSK_KEX2_1	277	279	PF00082	0.212
CLV_PCSK_KEX2_1	412	414	PF00082	0.568
CLV_PCSK_KEX2_1	448	450	PF00082	0.504
CLV_PCSK_KEX2_1	51	53	PF00082	0.212
CLV_PCSK_KEX2_1	89	91	PF00082	0.212
CLV_PCSK_PC1ET2_1	51	53	PF00082	0.212
CLV_PCSK_PC1ET2_1	89	91	PF00082	0.243
CLV_PCSK_PC7_1	444	450	PF00082	0.467
CLV_PCSK_PC7_1	85	91	PF00082	0.243
CLV_PCSK_SKI1_1	18	22	PF00082	0.212
CLV_PCSK_SKI1_1	185	189	PF00082	0.225
CLV_PCSK_SKI1_1	212	216	PF00082	0.305
CLV_PCSK_SKI1_1	277	281	PF00082	0.212
CLV_PCSK_SKI1_1	319	323	PF00082	0.593
CLV_PCSK_SKI1_1	474	478	PF00082	0.491
DEG_APCC_DBOX_1	211	219	PF00400	0.425
DEG_APCC_DBOX_1	49	57	PF00400	0.412
DOC_MAPK_gen_1	209	219	PF00069	0.415
DOC_MAPK_MEF2A_6	212	221	PF00069	0.412
DOC_MAPK_NFAT4_5	212	220	PF00069	0.412
DOC_PP4_FxxP_1	175	178	PF00568	0.425
DOC_SPAK_OSR1_1	278	282	PF12202	0.425
DOC_USP7_MATH_1	258	262	PF00917	0.503
DOC_USP7_UBL2_3	465	469	PF12436	0.485
LIG_14-3-3_CanoR_1	128	132	PF00244	0.412
LIG_14-3-3_CanoR_1	157	166	PF00244	0.492
LIG_14-3-3_CanoR_1	18	27	PF00244	0.412
LIG_14-3-3_CanoR_1	474	484	PF00244	0.633
LIG_BRCT_BRCA1_1	260	264	PF00533	0.503
LIG_BRCT_BRCA1_1	305	309	PF00533	0.446
LIG_CSL_BTD_1	284	287	PF09270	0.509
LIG_eIF4E_1	164	170	PF01652	0.412
LIG_FHA_1	33	39	PF00498	0.412
LIG_FHA_2	19	25	PF00498	0.412
LIG_FHA_2	65	71	PF00498	0.412
LIG_Integrin_isoDGR_2	143	145	PF01839	0.269
LIG_LIR_Apic_2	172	178	PF02991	0.425
LIG_LIR_Gen_1	163	171	PF02991	0.426
LIG_LIR_Gen_1	230	238	PF02991	0.412
LIG_LIR_Gen_1	80	87	PF02991	0.428
LIG_LIR_Nem_3	130	134	PF02991	0.412
LIG_LIR_Nem_3	14	20	PF02991	0.412
LIG_LIR_Nem_3	150	155	PF02991	0.308
LIG_LIR_Nem_3	163	169	PF02991	0.437
LIG_LIR_Nem_3	230	234	PF02991	0.412
LIG_LIR_Nem_3	283	289	PF02991	0.366
LIG_LIR_Nem_3	67	72	PF02991	0.412
LIG_LIR_Nem_3	80	84	PF02991	0.412
LIG_NRBOX	52	58	PF00104	0.412
LIG_PALB2_WD40_1	281	289	PF16756	0.492
LIG_PCNA_yPIPBox_3	3	12	PF02747	0.293
LIG_PCNA_yPIPBox_3	311	321	PF02747	0.402
LIG_Pex14_2	275	279	PF04695	0.412
LIG_Rb_pABgroove_1	165	173	PF01858	0.425
LIG_REV1ctd_RIR_1	272	282	PF16727	0.412
LIG_SH2_CRK	79	83	PF00017	0.412
LIG_SH2_SRC	171	174	PF00017	0.428
LIG_SH2_STAP1	72	76	PF00017	0.416
LIG_SH2_STAP1	79	83	PF00017	0.406
LIG_SH2_STAT5	154	157	PF00017	0.429
LIG_SH2_STAT5	81	84	PF00017	0.438
LIG_SH3_3	174	180	PF00018	0.412
LIG_SH3_4	179	186	PF00018	0.425
LIG_SUMO_SIM_anti_2	105	114	PF11976	0.341
LIG_SUMO_SIM_par_1	105	114	PF11976	0.341
LIG_SUMO_SIM_par_1	243	248	PF11976	0.413
LIG_SxIP_EBH_1	368	380	PF03271	0.515
LIG_TRAF2_1	384	387	PF00917	0.645
MOD_CK1_1	130	136	PF00069	0.417
MOD_CK1_1	328	334	PF00069	0.523
MOD_CK1_1	342	348	PF00069	0.775
MOD_CK1_1	354	360	PF00069	0.667
MOD_CK2_1	157	163	PF00069	0.503
MOD_CK2_1	18	24	PF00069	0.412
MOD_CK2_1	328	334	PF00069	0.576
MOD_CK2_1	381	387	PF00069	0.721
MOD_GlcNHglycan	223	226	PF01048	0.285
MOD_GlcNHglycan	305	308	PF01048	0.461
MOD_GSK3_1	153	160	PF00069	0.473
MOD_GSK3_1	281	288	PF00069	0.478
MOD_GSK3_1	303	310	PF00069	0.425
MOD_GSK3_1	85	92	PF00069	0.443
MOD_N-GLC_1	199	204	PF02516	0.225
MOD_N-GLC_1	258	263	PF02516	0.303
MOD_N-GLC_1	351	356	PF02516	0.719
MOD_N-GLC_1	4	9	PF02516	0.282
MOD_NEK2_1	153	158	PF00069	0.430
MOD_NEK2_1	221	226	PF00069	0.488
MOD_NEK2_1	4	9	PF00069	0.332
MOD_NEK2_1	476	481	PF00069	0.628
MOD_NEK2_2	127	132	PF00069	0.412
MOD_PIKK_1	157	163	PF00454	0.503
MOD_PIKK_1	334	340	PF00454	0.732
MOD_PIKK_1	351	357	PF00454	0.532
MOD_PK_1	371	377	PF00069	0.570
MOD_PKA_1	89	95	PF00069	0.486
MOD_PKA_2	127	133	PF00069	0.412
MOD_PKA_2	89	95	PF00069	0.486
MOD_Plk_1	162	168	PF00069	0.443
MOD_Plk_1	281	287	PF00069	0.499
MOD_Plk_1	4	10	PF00069	0.346
MOD_Plk_1	64	70	PF00069	0.412
MOD_Plk_2-3	389	395	PF00069	0.683
MOD_Plk_4	148	154	PF00069	0.415
MOD_Plk_4	162	168	PF00069	0.448
MOD_Plk_4	193	199	PF00069	0.444
MOD_Plk_4	281	287	PF00069	0.375
MOD_Plk_4	4	10	PF00069	0.346
MOD_Plk_4	64	70	PF00069	0.425
TRG_DiLeu_BaLyEn_6	397	402	PF01217	0.586
TRG_ENDOCYTIC_2	164	167	PF00928	0.476
TRG_ENDOCYTIC_2	79	82	PF00928	0.418
TRG_ER_diArg_1	211	213	PF00400	0.425
TRG_ER_diArg_1	277	279	PF00400	0.412
TRG_ER_diArg_1	447	449	PF00400	0.490
TRG_ER_diArg_1	50	53	PF00400	0.412
TRG_NES_CRM1_1	269	282	PF08389	0.485
TRG_Pf-PMV_PEXEL_1	400	404	PF00026	0.620

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8V8	Leptomonas seymouri	84%	100%
A0A0N1IIE8	Leptomonas seymouri	24%	88%
A0A1X0P272	Trypanosomatidae	55%	100%
A0A3R7N743	Trypanosoma rangeli	64%	97%
A4HI88	Leishmania braziliensis	88%	100%
A4I5G7	Leishmania infantum	99%	100%
C9ZQV3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	66%	100%
E9B0R5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
P40160	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	41%	100%
Q4Q7G8	Leishmania major	97%	100%
Q95Q34	Caenorhabditis elegans	47%	92%
Q9BVS4	Homo sapiens	43%	88%
Q9P7W5	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	46%	100%
V5B903	Trypanosoma cruzi	67%	100%