Cysteine protease

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Cysteine protease

Gene product:

AUT2/APG4/ATG4 cysteine peptidase, putative

Species:

Leishmania donovani

UniProt:

A0A3S7X2Y6_LEIDO

TriTrypDb:

LdBPK_300270.1 , LdCL_300007800 , LDHU3_30.0410

Length:

394

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	11
GO:0110165	cellular anatomical entity	1	11

Expansion

Sequence features

A0A3S7X2Y6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7X2Y6

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0006810	transport	3	11
GO:0006914	autophagy	3	10
GO:0008104	protein localization	4	11
GO:0008152	metabolic process	1	11
GO:0009056	catabolic process	2	10
GO:0009987	cellular process	1	11
GO:0015031	protein transport	4	11
GO:0019538	protein metabolic process	3	11
GO:0033036	macromolecule localization	2	11
GO:0043170	macromolecule metabolic process	3	11
GO:0044237	cellular metabolic process	2	10
GO:0044238	primary metabolic process	2	11
GO:0044248	cellular catabolic process	3	10
GO:0045184	establishment of protein localization	3	11
GO:0051179	localization	1	11
GO:0051234	establishment of localization	2	11
GO:0051641	cellular localization	2	11
GO:0061919	process utilizing autophagic mechanism	2	10
GO:0070727	cellular macromolecule localization	3	11
GO:0071702	organic substance transport	4	11
GO:0071704	organic substance metabolic process	2	11
GO:0071705	nitrogen compound transport	4	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:0000045	autophagosome assembly	6	1
GO:0006996	organelle organization	4	1
GO:0007033	vacuole organization	5	1
GO:0016043	cellular component organization	3	1
GO:0022607	cellular component assembly	4	1
GO:0070925	organelle assembly	5	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:1905037	autophagosome organization	6	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0008233	peptidase activity	3	11
GO:0008234	cysteine-type peptidase activity	4	11
GO:0016787	hydrolase activity	2	11
GO:0140096	catalytic activity, acting on a protein	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	377	381	PF00656	0.581
CLV_C14_Caspase3-7	49	53	PF00656	0.475
CLV_NRD_NRD_1	121	123	PF00675	0.316
CLV_NRD_NRD_1	353	355	PF00675	0.687
CLV_NRD_NRD_1	359	361	PF00675	0.697
CLV_NRD_NRD_1	94	96	PF00675	0.328
CLV_PCSK_KEX2_1	121	123	PF00082	0.316
CLV_PCSK_KEX2_1	139	141	PF00082	0.364
CLV_PCSK_KEX2_1	353	355	PF00082	0.689
CLV_PCSK_KEX2_1	361	363	PF00082	0.714
CLV_PCSK_KEX2_1	94	96	PF00082	0.266
CLV_PCSK_PC1ET2_1	139	141	PF00082	0.374
CLV_PCSK_PC1ET2_1	361	363	PF00082	0.635
CLV_PCSK_PC7_1	357	363	PF00082	0.580
CLV_PCSK_PC7_1	90	96	PF00082	0.214
CLV_PCSK_SKI1_1	121	125	PF00082	0.283
CLV_PCSK_SKI1_1	361	365	PF00082	0.701
CLV_PCSK_SKI1_1	39	43	PF00082	0.470
DEG_Nend_Nbox_1	1	3	PF02207	0.568
DOC_AGCK_PIF_1	45	50	PF00069	0.477
DOC_MAPK_gen_1	94	100	PF00069	0.475
DOC_MAPK_MEF2A_6	175	184	PF00069	0.528
DOC_MAPK_MEF2A_6	272	281	PF00069	0.515
DOC_PP1_RVXF_1	264	271	PF00149	0.434
DOC_PP2B_LxvP_1	356	359	PF13499	0.600
DOC_PP4_FxxP_1	327	330	PF00568	0.682
DOC_USP7_MATH_1	220	224	PF00917	0.433
DOC_USP7_MATH_1	254	258	PF00917	0.450
DOC_USP7_MATH_1	30	34	PF00917	0.431
DOC_WW_Pin1_4	127	132	PF00397	0.427
DOC_WW_Pin1_4	256	261	PF00397	0.480
DOC_WW_Pin1_4	326	331	PF00397	0.664
LIG_14-3-3_CanoR_1	188	195	PF00244	0.505
LIG_14-3-3_CanoR_1	353	357	PF00244	0.690
LIG_14-3-3_CanoR_1	75	81	PF00244	0.434
LIG_14-3-3_CanoR_1	94	99	PF00244	0.436
LIG_APCC_ABBA_1	96	101	PF00400	0.475
LIG_APCC_ABBAyCdc20_2	95	101	PF00400	0.475
LIG_BRCT_BRCA1_1	222	226	PF00533	0.515
LIG_BRCT_BRCA1_1	296	300	PF00533	0.433
LIG_BRCT_BRCA1_1	9	13	PF00533	0.478
LIG_BRCT_BRCA1_1	96	100	PF00533	0.501
LIG_Clathr_ClatBox_1	234	238	PF01394	0.434
LIG_deltaCOP1_diTrp_1	7	13	PF00928	0.462
LIG_EH1_1	108	116	PF00400	0.477
LIG_FHA_1	13	19	PF00498	0.512
LIG_FHA_1	146	152	PF00498	0.528
LIG_FHA_1	257	263	PF00498	0.425
LIG_FHA_1	353	359	PF00498	0.632
LIG_FHA_1	42	48	PF00498	0.333
LIG_FHA_1	59	65	PF00498	0.515
LIG_FHA_2	282	288	PF00498	0.488
LIG_FHA_2	306	312	PF00498	0.570
LIG_FHA_2	47	53	PF00498	0.515
LIG_LIR_Apic_2	127	131	PF02991	0.438
LIG_LIR_Apic_2	324	330	PF02991	0.680
LIG_LIR_Gen_1	198	207	PF02991	0.478
LIG_LIR_Gen_1	275	283	PF02991	0.476
LIG_LIR_Gen_1	287	296	PF02991	0.386
LIG_LIR_Gen_1	297	304	PF02991	0.359
LIG_LIR_Gen_1	387	394	PF02991	0.681
LIG_LIR_Gen_1	42	50	PF02991	0.422
LIG_LIR_Gen_1	52	60	PF02991	0.331
LIG_LIR_Gen_1	7	17	PF02991	0.539
LIG_LIR_Nem_3	198	203	PF02991	0.478
LIG_LIR_Nem_3	223	227	PF02991	0.421
LIG_LIR_Nem_3	275	281	PF02991	0.463
LIG_LIR_Nem_3	287	292	PF02991	0.451
LIG_LIR_Nem_3	297	303	PF02991	0.424
LIG_LIR_Nem_3	387	393	PF02991	0.681
LIG_LIR_Nem_3	42	48	PF02991	0.422
LIG_LIR_Nem_3	52	57	PF02991	0.332
LIG_LIR_Nem_3	67	73	PF02991	0.381
LIG_LIR_Nem_3	7	12	PF02991	0.528
LIG_MLH1_MIPbox_1	222	226	PF16413	0.515
LIG_MLH1_MIPbox_1	9	13	PF16413	0.458
LIG_MLH1_MIPbox_1	96	100	PF16413	0.501
LIG_PCNA_PIPBox_1	78	87	PF02747	0.515
LIG_PCNA_yPIPBox_3	75	85	PF02747	0.477
LIG_Pex14_1	9	13	PF04695	0.514
LIG_SH2_SRC	236	239	PF00017	0.450
LIG_SH2_STAP1	166	170	PF00017	0.515
LIG_SH2_STAT5	200	203	PF00017	0.496
LIG_SH2_STAT5	225	228	PF00017	0.463
LIG_SH2_STAT5	236	239	PF00017	0.449
LIG_SH2_STAT5	4	7	PF00017	0.549
LIG_SUMO_SIM_anti_2	153	160	PF11976	0.479
LIG_SUMO_SIM_anti_2	179	186	PF11976	0.454
LIG_SUMO_SIM_par_1	179	186	PF11976	0.472
LIG_TRAF2_1	33	36	PF00917	0.494
LIG_TRAF2_2	330	335	PF00917	0.517
LIG_TRFH_1	235	239	PF08558	0.475
MOD_CK1_1	103	109	PF00069	0.509
MOD_CK1_1	305	311	PF00069	0.454
MOD_CK2_1	282	288	PF00069	0.452
MOD_CK2_1	30	36	PF00069	0.408
MOD_CK2_1	340	346	PF00069	0.709
MOD_GlcNHglycan	101	105	PF01048	0.332
MOD_GlcNHglycan	159	162	PF01048	0.211
MOD_GlcNHglycan	189	192	PF01048	0.325
MOD_GlcNHglycan	252	255	PF01048	0.322
MOD_GlcNHglycan	26	29	PF01048	0.285
MOD_GlcNHglycan	9	12	PF01048	0.484
MOD_GSK3_1	103	110	PF00069	0.510
MOD_GSK3_1	183	190	PF00069	0.515
MOD_GSK3_1	246	253	PF00069	0.458
MOD_GSK3_1	254	261	PF00069	0.426
MOD_GSK3_1	268	275	PF00069	0.384
MOD_GSK3_1	326	333	PF00069	0.592
MOD_N-GLC_1	229	234	PF02516	0.274
MOD_N-GLC_2	162	164	PF02516	0.315
MOD_NEK2_1	114	119	PF00069	0.475
MOD_NEK2_1	156	161	PF00069	0.440
MOD_NEK2_1	281	286	PF00069	0.520
MOD_NEK2_1	339	344	PF00069	0.661
MOD_NEK2_1	41	46	PF00069	0.373
MOD_NEK2_1	73	78	PF00069	0.426
MOD_PIKK_1	129	135	PF00454	0.434
MOD_PIKK_1	193	199	PF00454	0.452
MOD_PIKK_1	229	235	PF00454	0.501
MOD_PIKK_1	302	308	PF00454	0.568
MOD_PIKK_1	74	80	PF00454	0.434
MOD_PKA_1	94	100	PF00069	0.501
MOD_PKA_2	187	193	PF00069	0.513
MOD_PKA_2	352	358	PF00069	0.681
MOD_PKA_2	74	80	PF00069	0.434
MOD_PKA_2	94	100	PF00069	0.419
MOD_Plk_1	229	235	PF00069	0.474
MOD_Plk_1	273	279	PF00069	0.421
MOD_Plk_2-3	386	392	PF00069	0.683
MOD_Plk_4	196	202	PF00069	0.486
MOD_Plk_4	273	279	PF00069	0.451
MOD_Plk_4	323	329	PF00069	0.670
MOD_Plk_4	41	47	PF00069	0.418
MOD_Plk_4	94	100	PF00069	0.505
MOD_ProDKin_1	127	133	PF00069	0.427
MOD_ProDKin_1	256	262	PF00069	0.480
MOD_ProDKin_1	326	332	PF00069	0.664
MOD_SUMO_for_1	124	127	PF00179	0.515
MOD_SUMO_for_1	146	149	PF00179	0.475
MOD_SUMO_for_1	374	377	PF00179	0.527
TRG_DiLeu_BaLyEn_6	59	64	PF01217	0.515
TRG_ENDOCYTIC_2	200	203	PF00928	0.515
TRG_ENDOCYTIC_2	224	227	PF00928	0.421
TRG_ER_diArg_1	317	320	PF00400	0.565
TRG_ER_diArg_1	359	362	PF00400	0.653
TRG_ER_diArg_1	94	96	PF00400	0.458
TRG_NLS_MonoExtC_3	359	364	PF00514	0.625
TRG_NLS_MonoExtN_4	357	364	PF00514	0.580

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A098DRK7	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	26%	84%
A0A0N0P8C0	Leptomonas seymouri	65%	98%
A0A0S4JSC7	Bodo saltans	37%	100%
A0A1X0P2H1	Trypanosomatidae	40%	100%
A0A3R7NRV7	Trypanosoma rangeli	42%	100%
A0A422NI42	Trypanosoma rangeli	25%	100%
A1CJ08	Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107)	26%	98%
A2QY50	Aspergillus niger (strain CBS 513.88 / FGSC A1513)	27%	98%
A4HHX1	Leishmania braziliensis	84%	100%
A4I521	Leishmania infantum	100%	100%
A7KAI3	Pichia angusta	31%	77%
E2RDP2	Canis lupus familiaris	25%	83%
E9B0F1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
K8ESC5	Caenorhabditis elegans	25%	82%
Q0U199	Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173)	27%	84%
Q1E5M9	Coccidioides immitis (strain RS)	28%	91%
Q2HH40	Chaetomium globosum (strain ATCC 6205 / CBS 148.51 / DSM 1962 / NBRC 6347 / NRRL 1970)	26%	88%
Q2U5B0	Aspergillus oryzae (strain ATCC 42149 / RIB 40)	27%	97%
Q4Q7T2	Leishmania major	94%	100%
Q523C3	Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)	28%	80%
Q5B7L0	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	25%	98%
Q684M2	Sus scrofa	26%	84%
Q68FJ9	Xenopus laevis	22%	84%
Q6CH28	Yarrowia lipolytica (strain CLIB 122 / E 150)	24%	72%
Q6FP20	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	21%	82%
Q6PZ02	Gallus gallus	25%	100%
Q86TL0	Homo sapiens	26%	83%
Q86ZL5	Podospora anserina	26%	79%
Q8BGE6	Mus musculus	24%	100%
Q8BGV9	Mus musculus	26%	83%
Q9P373	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	100%
Q9U1N6	Caenorhabditis elegans	24%	76%
Q9Y4P1	Homo sapiens	25%	100%
V5AXZ8	Trypanosoma cruzi	26%	100%
V5BGZ3	Trypanosoma cruzi	40%	100%
W0TGM7	Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275)	25%	87%