ATP-dependent 6-phosphofructokinase

Quick info Annotations Localization Abundance Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ATP-dependent 6-phosphofructokinase, glycosomal

Species:

Leishmania donovani

UniProt:

A0A3S7X2Y4_LEIDO

TriTrypDb:

LdBPK_292620.1 , LdCL_290032300 , LDHU3_29.3880

Length:

486

Annotations

Annotations by Jardim et al.

Glycolysis Proteins, ATP-dependent 6-phosphofructokinase pfk

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 8

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 16

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005777	peroxisome	6	10
GO:0020015	glycosome	7	10
GO:0042579	microbody	5	10
GO:0043226	organelle	2	10
GO:0043227	membrane-bounded organelle	3	10
GO:0043229	intracellular organelle	3	10
GO:0043231	intracellular membrane-bounded organelle	4	10
GO:0110165	cellular anatomical entity	1	11
GO:0005737	cytoplasm	2	2
GO:0016020	membrane	2	1

Abundance

Amastigote (protein)


Source	Evidence on protein	Close homologs
Pescher et al. (upgregulation)	no	yes: 0

Promastigote and amastigote


Source	Evidence on protein	Close homologs
Lahav et al. - mRNA - Protein

Expansion

Sequence features

A0A3S7X2Y4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7X2Y4

Function

Biological processes
Term	Name	Level	Count
GO:0006002	fructose 6-phosphate metabolic process	4	12
GO:0006793	phosphorus metabolic process	3	12
GO:0006796	phosphate-containing compound metabolic process	4	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0019637	organophosphate metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0071704	organic substance metabolic process	2	12
GO:1901135	carbohydrate derivative metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003824	catalytic activity	1	12
GO:0003872	6-phosphofructokinase activity	6	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	11
GO:0008443	phosphofructokinase activity	5	12
GO:0016301	kinase activity	4	12
GO:0016740	transferase activity	2	12
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	12
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	12
GO:0017076	purine nucleotide binding	4	11
GO:0019200	carbohydrate kinase activity	5	12
GO:0030554	adenyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	11
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	386	390	PF00656	0.420
CLV_C14_Caspase3-7	65	69	PF00656	0.592
CLV_NRD_NRD_1	212	214	PF00675	0.209
CLV_NRD_NRD_1	309	311	PF00675	0.209
CLV_NRD_NRD_1	432	434	PF00675	0.294
CLV_NRD_NRD_1	465	467	PF00675	0.428
CLV_NRD_NRD_1	85	87	PF00675	0.421
CLV_PCSK_FUR_1	126	130	PF00082	0.269
CLV_PCSK_KEX2_1	128	130	PF00082	0.209
CLV_PCSK_KEX2_1	211	213	PF00082	0.209
CLV_PCSK_KEX2_1	309	311	PF00082	0.209
CLV_PCSK_KEX2_1	432	434	PF00082	0.309
CLV_PCSK_KEX2_1	465	467	PF00082	0.406
CLV_PCSK_PC1ET2_1	128	130	PF00082	0.209
CLV_PCSK_PC1ET2_1	211	213	PF00082	0.220
CLV_PCSK_PC7_1	305	311	PF00082	0.295
CLV_PCSK_SKI1_1	115	119	PF00082	0.209
CLV_PCSK_SKI1_1	201	205	PF00082	0.213
CLV_PCSK_SKI1_1	426	430	PF00082	0.304
CLV_PCSK_SKI1_1	43	47	PF00082	0.615
CLV_PCSK_SKI1_1	438	442	PF00082	0.291
CLV_PCSK_SKI1_1	446	450	PF00082	0.156
DEG_APCC_DBOX_1	464	472	PF00400	0.412
DEG_Nend_UBRbox_2	1	3	PF02207	0.663
DOC_CYCLIN_RxL_1	84	95	PF00134	0.452
DOC_MAPK_gen_1	126	133	PF00069	0.469
DOC_MAPK_gen_1	185	192	PF00069	0.495
DOC_MAPK_gen_1	211	219	PF00069	0.409
DOC_MAPK_gen_1	342	349	PF00069	0.420
DOC_MAPK_gen_1	443	452	PF00069	0.351
DOC_MAPK_gen_1	465	473	PF00069	0.429
DOC_MAPK_HePTP_8	413	425	PF00069	0.294
DOC_MAPK_MEF2A_6	185	192	PF00069	0.409
DOC_MAPK_MEF2A_6	342	349	PF00069	0.399
DOC_MAPK_MEF2A_6	416	425	PF00069	0.298
DOC_MAPK_MEF2A_6	443	452	PF00069	0.351
DOC_PP1_RVXF_1	127	134	PF00149	0.409
DOC_PP1_RVXF_1	85	92	PF00149	0.342
DOC_PP4_FxxP_1	220	223	PF00568	0.420
DOC_USP7_MATH_1	427	431	PF00917	0.375
DOC_USP7_UBL2_3	142	146	PF12436	0.495
LIG_14-3-3_CanoR_1	115	120	PF00244	0.409
LIG_14-3-3_CanoR_1	432	438	PF00244	0.419
LIG_APCC_ABBA_1	204	209	PF00400	0.495
LIG_BIR_III_4	337	341	PF00653	0.412
LIG_DLG_GKlike_1	433	441	PF00625	0.426
LIG_eIF4E_1	417	423	PF01652	0.362
LIG_FHA_1	118	124	PF00498	0.493
LIG_FHA_1	396	402	PF00498	0.428
LIG_FHA_1	407	413	PF00498	0.238
LIG_FHA_1	447	453	PF00498	0.366
LIG_FHA_1	7	13	PF00498	0.435
LIG_FHA_2	145	151	PF00498	0.409
LIG_FHA_2	203	209	PF00498	0.452
LIG_FHA_2	21	27	PF00498	0.378
LIG_FHA_2	239	245	PF00498	0.409
LIG_LIR_Apic_2	14	18	PF02991	0.539
LIG_LIR_Apic_2	370	376	PF02991	0.409
LIG_LIR_Nem_3	414	420	PF02991	0.385
LIG_PDZ_Class_1	481	486	PF00595	0.433
LIG_Pex14_2	325	329	PF04695	0.409
LIG_SH2_CRK	15	19	PF00017	0.540
LIG_SH2_CRK	37	41	PF00017	0.442
LIG_SH2_GRB2like	37	40	PF00017	0.392
LIG_SH2_PTP2	373	376	PF00017	0.409
LIG_SH2_PTP2	420	423	PF00017	0.295
LIG_SH2_SRC	207	210	PF00017	0.495
LIG_SH2_SRC	254	257	PF00017	0.420
LIG_SH2_SRC	373	376	PF00017	0.409
LIG_SH2_STAP1	254	258	PF00017	0.420
LIG_SH2_STAT5	373	376	PF00017	0.409
LIG_SH2_STAT5	420	423	PF00017	0.295
LIG_SH3_3	170	176	PF00018	0.407
LIG_SH3_3	291	297	PF00018	0.409
LIG_SUMO_SIM_par_1	317	323	PF11976	0.411
LIG_SUMO_SIM_par_1	397	403	PF11976	0.449
LIG_TRFH_1	420	424	PF08558	0.298
LIG_UBA3_1	421	426	PF00899	0.303
LIG_UBA3_1	478	485	PF00899	0.426
MOD_CK1_1	20	26	PF00069	0.434
MOD_CK1_1	233	239	PF00069	0.261
MOD_CK1_1	94	100	PF00069	0.433
MOD_CK2_1	144	150	PF00069	0.261
MOD_CK2_1	202	208	PF00069	0.328
MOD_CK2_1	238	244	PF00069	0.296
MOD_GlcNHglycan	273	276	PF01048	0.273
MOD_GlcNHglycan	284	287	PF01048	0.261
MOD_GlcNHglycan	337	341	PF01048	0.275
MOD_GSK3_1	113	120	PF00069	0.239
MOD_GSK3_1	138	145	PF00069	0.290
MOD_GSK3_1	162	169	PF00069	0.261
MOD_GSK3_1	278	285	PF00069	0.346
MOD_GSK3_1	391	398	PF00069	0.381
MOD_GSK3_1	446	453	PF00069	0.472
MOD_N-GLC_2	289	291	PF02516	0.277
MOD_NEK2_1	162	167	PF00069	0.280
MOD_NEK2_1	179	184	PF00069	0.190
MOD_NEK2_1	230	235	PF00069	0.261
MOD_NEK2_1	311	316	PF00069	0.261
MOD_NEK2_1	391	396	PF00069	0.281
MOD_NEK2_2	202	207	PF00069	0.369
MOD_NEK2_2	367	372	PF00069	0.261
MOD_NEK2_2	411	416	PF00069	0.343
MOD_NEK2_2	427	432	PF00069	0.336
MOD_PIKK_1	196	202	PF00454	0.261
MOD_PKA_2	271	277	PF00069	0.261
MOD_Plk_1	25	31	PF00069	0.491
MOD_Plk_1	446	452	PF00069	0.358
MOD_Plk_1	94	100	PF00069	0.370
MOD_Plk_4	162	168	PF00069	0.261
MOD_Plk_4	179	185	PF00069	0.261
MOD_Plk_4	202	208	PF00069	0.369
MOD_Plk_4	299	305	PF00069	0.322
MOD_Plk_4	354	360	PF00069	0.311
MOD_Plk_4	374	380	PF00069	0.176
MOD_SUMO_for_1	351	354	PF00179	0.381
TRG_DiLeu_BaLyEn_6	302	307	PF01217	0.381
TRG_ENDOCYTIC_2	137	140	PF00928	0.261
TRG_ENDOCYTIC_2	420	423	PF00928	0.282
TRG_ER_diArg_1	309	311	PF00400	0.261
TRG_ER_diArg_1	431	433	PF00400	0.319
TRG_ER_diArg_1	464	466	PF00400	0.426
TRG_NES_CRM1_1	462	476	PF08389	0.494
TRG_Pf-PMV_PEXEL_1	309	313	PF00026	0.330

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PBL6	Leptomonas seymouri	90%	100%
A0A0S4IQ47	Bodo saltans	60%	98%
A0A1X0P916	Trypanosomatidae	72%	100%
A0A3R7N9H0	Trypanosoma rangeli	72%	100%
A2RE65	Streptococcus pyogenes serotype M5 (strain Manfredo)	25%	100%
A2RKA2	Lactococcus lactis subsp. cremoris (strain MG1363)	26%	100%
A3CM69	Streptococcus sanguinis (strain SK36)	25%	100%
A3N1C8	Actinobacillus pleuropneumoniae serotype 5b (strain L20)	29%	100%
A4HHQ0	Leishmania braziliensis	95%	100%
A4I4W5	Leishmania infantum	100%	100%
A8AXW1	Streptococcus gordonii (strain Challis / ATCC 35105 / BCRC 15272 / CH1 / DL1 / V288)	25%	100%
A9WCU2	Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl)	34%	100%
B0BQ64	Actinobacillus pleuropneumoniae serotype 3 (strain JL03)	29%	100%
B1IB66	Streptococcus pneumoniae (strain Hungary19A-6)	25%	100%
B2IP53	Streptococcus pneumoniae (strain CGSP14)	25%	100%
B2V7W7	Sulfurihydrogenibium sp. (strain YO3AOP1)	29%	100%
B3GY11	Actinobacillus pleuropneumoniae serotype 7 (strain AP76)	29%	100%
B4U2M2	Streptococcus equi subsp. zooepidemicus (strain MGCS10565)	25%	100%
B5E3Z8	Streptococcus pneumoniae serotype 19F (strain G54)	25%	100%
B5XLV6	Streptococcus pyogenes serotype M49 (strain NZ131)	25%	100%
B8D1K5	Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)	31%	100%
B8GAA4	Chloroflexus aggregans (strain MD-66 / DSM 9485)	34%	100%
B8ZNU0	Streptococcus pneumoniae (strain ATCC 700669 / Spain 23F-1)	25%	100%
B9LHK4	Chloroflexus aurantiacus (strain ATCC 29364 / DSM 637 / Y-400-fl)	34%	100%
C0M8V6	Streptococcus equi subsp. equi (strain 4047)	25%	100%
C0MGJ9	Streptococcus equi subsp. zooepidemicus (strain H70)	25%	100%
C0QQX4	Persephonella marina (strain DSM 14350 / EX-H1)	30%	100%
C1C6M8	Streptococcus pneumoniae (strain 70585)	25%	100%
C1CDP4	Streptococcus pneumoniae (strain JJA)	25%	100%
C1CJY4	Streptococcus pneumoniae (strain P1031)	25%	100%
C1CRZ7	Streptococcus pneumoniae (strain Taiwan19F-14)	25%	100%
C9ZKZ6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	71%	100%
D7GCU5	Propionibacterium freudenreichii subsp. shermanii (strain ATCC 9614 / DSM 4902 / CIP 103027 / NCIMB 8099 / CIRM-BIA1)	27%	100%
E0RTD9	Spirochaeta thermophila (strain ATCC 49972 / DSM 6192 / RI 19.B1)	26%	100%
E9AEB3	Leishmania major	99%	100%
E9ALH3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
G4RK16	Thermoproteus tenax (strain ATCC 35583 / DSM 2078 / JCM 9277 / NBRC 100435 / Kra 1)	29%	100%
G4STG9	Methylotuvimicrobium alcaliphilum (strain DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z)	25%	100%
O08333	Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)	33%	100%
O15648	Trypanosoma brucei brucei	71%	100%
O33106	Mycobacterium leprae (strain TN)	33%	100%
O51669	Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)	42%	100%
O61068	Trichomonas vaginalis	29%	100%
O83146	Treponema pallidum (strain Nichols)	41%	100%
P0DD02	Streptococcus pyogenes serotype M3 (strain ATCC BAA-595 / MGAS315)	25%	100%
P0DD03	Streptococcus pyogenes serotype M3 (strain SSI-1)	25%	100%
P0DOB5	Lactococcus lactis subsp. lactis (strain IL1403)	26%	100%
P0DOB6	Lactococcus lactis subsp. lactis	26%	100%
P0DOB7	Lactococcus lactis subsp. lactis	26%	100%
P20275	Spiroplasma citri	29%	100%
P29495	Propionibacterium freudenreichii subsp. shermanii (strain ATCC 9614 / DSM 4902 / CIP 103027 / NCIMB 8099 / CIRM-BIA1)	24%	100%
P65691	Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)	34%	100%
P65696	Streptococcus agalactiae serotype III (strain NEM316)	24%	100%
P65697	Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R)	24%	100%
P72830	Synechocystis sp. (strain PCC 6803 / Kazusa)	29%	100%
P9WID6	Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)	34%	100%
P9WID7	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	34%	100%
Q02YK5	Lactococcus lactis subsp. cremoris (strain SK11)	26%	100%
Q03KB7	Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9)	26%	100%
Q04L24	Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466)	26%	100%
Q11RE2	Cytophaga hutchinsonii (strain ATCC 33406 / DSM 1761 / CIP 103989 / NBRC 15051 / NCIMB 9469 / D465)	26%	100%
Q1J694	Streptococcus pyogenes serotype M4 (strain MGAS10750)	25%	100%
Q1JBF7	Streptococcus pyogenes serotype M12 (strain MGAS2096)	25%	100%
Q1JGH6	Streptococcus pyogenes serotype M2 (strain MGAS10270)	25%	100%
Q1JLD9	Streptococcus pyogenes serotype M12 (strain MGAS9429)	25%	100%
Q27651	Entamoeba histolytica	43%	100%
Q27705	Naegleria fowleri	30%	100%
Q3K1E6	Streptococcus agalactiae serotype Ia (strain ATCC 27591 / A909 / CDC SS700)	24%	100%
Q3KSV5	Methylomonas methanica	26%	100%
Q48T86	Streptococcus pyogenes serotype M28 (strain MGAS6180)	25%	100%
Q4E657	Trypanosoma cruzi (strain CL Brener)	72%	100%
Q59126	Amycolatopsis methanolica	34%	100%
Q5XBV2	Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)	25%	100%
Q8A624	Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)	28%	100%
Q8A8R5	Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)	29%	100%
Q8DJB1	Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1)	29%	100%
Q8DQ85	Streptococcus pneumoniae (strain ATCC BAA-255 / R6)	26%	100%
Q8NR14	Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)	31%	100%
Q8P0S6	Streptococcus pyogenes serotype M18 (strain MGAS8232)	25%	100%
Q8RQN5	Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)	30%	100%
Q8VU09	Amycolatopsis methanolica	41%	100%
Q8VYN6	Arabidopsis thaliana	44%	91%
Q8WPP2	Trypanoplasma borreli	57%	99%
Q8YKG3	Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576)	27%	100%
Q94AA4	Arabidopsis thaliana	43%	99%
Q97RC6	Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)	25%	100%
Q98PW8	Mycoplasmopsis pulmonis (strain UAB CTIP)	24%	100%
Q99ZD0	Streptococcus pyogenes serotype M1	25%	100%
Q9AGC0	Amycolatopsis mediterranei (strain S699)	33%	100%
Q9BIC6	Leishmania donovani	100%	100%
Q9C5J7	Arabidopsis thaliana	43%	100%
Q9FC99	Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)	31%	100%
Q9FIK0	Arabidopsis thaliana	38%	100%
Q9FKG3	Arabidopsis thaliana	43%	92%
Q9KH71	Dictyoglomus thermophilum	31%	100%
Q9L1L8	Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)	33%	100%
Q9L9E3	Streptococcus thermophilus	26%	100%
Q9M076	Arabidopsis thaliana	43%	100%
Q9M0F9	Arabidopsis thaliana	43%	100%
Q9NGP6	Mastigamoeba balamuthi	24%	100%
Q9RWN1	Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)	28%	100%
Q9WYC5	Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)	30%	100%
V5DTU9	Trypanosoma cruzi	72%	87%