rRNA adenine N(6)-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ribosomal RNA adenine dimethylase family protein, putative

Species:

Leishmania donovani

UniProt:

A0A3S7X2X5_LEIDO

TriTrypDb:

LdBPK_300210.1 , LdCL_300007100 , LDHU3_30.0330

Length:

374

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005730	nucleolus	5	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	2
GO:0016020	membrane	2	1

Expansion

Sequence features

A0A3S7X2X5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7X2X5

Function

Biological processes
Term	Name	Level	Count
GO:0000154	rRNA modification	6	1
GO:0001510	RNA methylation	4	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006364	rRNA processing	8	1
GO:0006396	RNA processing	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009451	RNA modification	5	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0016072	rRNA metabolic process	7	1
GO:0031167	rRNA methylation	5	1
GO:0032259	methylation	2	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000179	rRNA (adenine-N6,N6-)-dimethyltransferase activity	6	12
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0008168	methyltransferase activity	4	12
GO:0008170	N-methyltransferase activity	5	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0008649	rRNA methyltransferase activity	5	12
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	12
GO:0016433	rRNA (adenine) methyltransferase activity	6	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140102	catalytic activity, acting on a rRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	272	274	PF00675	0.245
CLV_NRD_NRD_1	298	300	PF00675	0.541
CLV_NRD_NRD_1	43	45	PF00675	0.664
CLV_PCSK_KEX2_1	137	139	PF00082	0.248
CLV_PCSK_KEX2_1	349	351	PF00082	0.358
CLV_PCSK_KEX2_1	43	45	PF00082	0.768
CLV_PCSK_KEX2_1	54	56	PF00082	0.584
CLV_PCSK_PC1ET2_1	137	139	PF00082	0.248
CLV_PCSK_PC1ET2_1	349	351	PF00082	0.358
CLV_PCSK_PC1ET2_1	54	56	PF00082	0.666
CLV_PCSK_SKI1_1	181	185	PF00082	0.273
CLV_PCSK_SKI1_1	268	272	PF00082	0.248
CLV_PCSK_SKI1_1	30	34	PF00082	0.610
CLV_PCSK_SKI1_1	315	319	PF00082	0.741
CLV_PCSK_SKI1_1	335	339	PF00082	0.420
DEG_APCC_DBOX_1	334	342	PF00400	0.437
DEG_SPOP_SBC_1	316	320	PF00917	0.674
DOC_CKS1_1	140	145	PF01111	0.473
DOC_CKS1_1	183	188	PF01111	0.553
DOC_CYCLIN_RxL_1	265	272	PF00134	0.498
DOC_CYCLIN_RxL_1	332	340	PF00134	0.432
DOC_CYCLIN_yCln2_LP_2	80	86	PF00134	0.448
DOC_MAPK_gen_1	252	260	PF00069	0.373
DOC_MAPK_gen_1	3	12	PF00069	0.580
DOC_MAPK_MEF2A_6	252	260	PF00069	0.448
DOC_MAPK_MEF2A_6	96	104	PF00069	0.448
DOC_MAPK_RevD_3	286	300	PF00069	0.476
DOC_PP1_RVXF_1	119	126	PF00149	0.448
DOC_PP1_RVXF_1	22	28	PF00149	0.572
DOC_PP4_FxxP_1	236	239	PF00568	0.448
DOC_USP7_MATH_1	143	147	PF00917	0.489
DOC_USP7_MATH_1	173	177	PF00917	0.448
DOC_USP7_MATH_1	223	227	PF00917	0.460
DOC_USP7_MATH_1	316	320	PF00917	0.667
DOC_USP7_UBL2_3	26	30	PF12436	0.633
DOC_USP7_UBL2_3	92	96	PF12436	0.448
DOC_WW_Pin1_4	139	144	PF00397	0.448
DOC_WW_Pin1_4	182	187	PF00397	0.553
DOC_WW_Pin1_4	35	40	PF00397	0.725
LIG_14-3-3_CanoR_1	214	218	PF00244	0.448
LIG_14-3-3_CanoR_1	224	231	PF00244	0.448
LIG_BRCT_BRCA1_1	278	282	PF00533	0.448
LIG_FHA_1	106	112	PF00498	0.448
LIG_FHA_1	27	33	PF00498	0.669
LIG_FHA_1	96	102	PF00498	0.448
LIG_FHA_2	44	50	PF00498	0.701
LIG_LIR_Apic_2	235	239	PF02991	0.443
LIG_LIR_Gen_1	209	217	PF02991	0.449
LIG_LIR_Nem_3	157	163	PF02991	0.447
LIG_LIR_Nem_3	209	215	PF02991	0.449
LIG_LIR_Nem_3	261	266	PF02991	0.448
LIG_LIR_Nem_3	293	298	PF02991	0.389
LIG_LIR_Nem_3	332	337	PF02991	0.412
LIG_Pex14_2	195	199	PF04695	0.448
LIG_Pex14_2	71	75	PF04695	0.460
LIG_PTB_Apo_2	166	173	PF02174	0.448
LIG_PTB_Apo_2	69	76	PF02174	0.316
LIG_PTB_Phospho_1	166	172	PF10480	0.448
LIG_Rb_pABgroove_1	286	294	PF01858	0.418
LIG_SH2_CRK	212	216	PF00017	0.448
LIG_SH2_SRC	172	175	PF00017	0.448
LIG_SH2_STAP1	292	296	PF00017	0.446
LIG_SH2_STAT5	162	165	PF00017	0.427
LIG_SH2_STAT5	212	215	PF00017	0.440
LIG_SH2_STAT5	295	298	PF00017	0.380
LIG_SH3_1	248	254	PF00018	0.448
LIG_SH3_3	248	254	PF00018	0.448
LIG_UBA3_1	228	233	PF00899	0.432
LIG_UBA3_1	359	368	PF00899	0.442
MOD_CDK_SPxxK_3	182	189	PF00069	0.553
MOD_CK1_1	216	222	PF00069	0.453
MOD_CK1_1	232	238	PF00069	0.394
MOD_CK1_1	319	325	PF00069	0.652
MOD_CK1_1	327	333	PF00069	0.488
MOD_CK2_1	43	49	PF00069	0.701
MOD_GlcNHglycan	13	16	PF01048	0.583
MOD_GlcNHglycan	175	178	PF01048	0.239
MOD_GlcNHglycan	311	314	PF01048	0.732
MOD_GlcNHglycan	321	324	PF01048	0.654
MOD_GlcNHglycan	57	60	PF01048	0.634
MOD_GlcNHglycan	66	69	PF01048	0.503
MOD_GSK3_1	105	112	PF00069	0.458
MOD_GSK3_1	139	146	PF00069	0.473
MOD_GSK3_1	26	33	PF00069	0.599
MOD_GSK3_1	277	284	PF00069	0.448
MOD_GSK3_1	315	322	PF00069	0.667
MOD_GSK3_1	55	62	PF00069	0.621
MOD_N-GLC_1	109	114	PF02516	0.248
MOD_NEK2_1	115	120	PF00069	0.471
MOD_NEK2_1	229	234	PF00069	0.448
MOD_NEK2_1	269	274	PF00069	0.529
MOD_NEK2_1	317	322	PF00069	0.701
MOD_NEK2_1	337	342	PF00069	0.377
MOD_NEK2_1	34	39	PF00069	0.653
MOD_PIKK_1	216	222	PF00454	0.457
MOD_PIKK_1	62	68	PF00454	0.574
MOD_PK_1	277	283	PF00069	0.448
MOD_PKA_1	276	282	PF00069	0.448
MOD_PKA_1	43	49	PF00069	0.678
MOD_PKA_1	54	60	PF00069	0.673
MOD_PKA_2	213	219	PF00069	0.448
MOD_PKA_2	223	229	PF00069	0.448
MOD_PKA_2	43	49	PF00069	0.775
MOD_PKA_2	54	60	PF00069	0.537
MOD_Plk_1	242	248	PF00069	0.448
MOD_Plk_4	242	248	PF00069	0.448
MOD_Plk_4	277	283	PF00069	0.448
MOD_Plk_4	337	343	PF00069	0.314
MOD_Plk_4	358	364	PF00069	0.434
MOD_ProDKin_1	139	145	PF00069	0.448
MOD_ProDKin_1	182	188	PF00069	0.553
MOD_ProDKin_1	35	41	PF00069	0.724
MOD_SUMO_for_1	240	243	PF00179	0.448
MOD_SUMO_rev_2	244	254	PF00179	0.448
TRG_DiLeu_BaEn_1	262	267	PF01217	0.448
TRG_ENDOCYTIC_2	212	215	PF00928	0.448
TRG_ENDOCYTIC_2	298	301	PF00928	0.434
TRG_ER_diArg_1	350	353	PF00400	0.399
TRG_NLS_MonoExtC_3	272	277	PF00514	0.448
TRG_Pf-PMV_PEXEL_1	335	339	PF00026	0.387
TRG_Pf-PMV_PEXEL_1	352	356	PF00026	0.328

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IB33	Leptomonas seymouri	87%	100%
A0A0S4JJ91	Bodo saltans	68%	100%
A0A1X0P2H9	Trypanosomatidae	73%	100%
A0A422N140	Trypanosoma rangeli	72%	100%
A2RCH2	Streptococcus pyogenes serotype M5 (strain Manfredo)	29%	100%
A3CQN5	Streptococcus sanguinis (strain SK36)	33%	100%
A4HHW5	Leishmania braziliensis	88%	98%
A4I515	Leishmania infantum	100%	100%
B1AJP2	Ureaplasma parvum serovar 3 (strain ATCC 27815 / 27 / NCTC 11736)	32%	100%
B1HS82	Lysinibacillus sphaericus (strain C3-41)	30%	100%
B1KRY8	Clostridium botulinum (strain Loch Maree / Type A3)	32%	100%
B2AH89	Cupriavidus taiwanensis (strain DSM 17343 / BCRC 17206 / CCUG 44338 / CIP 107171 / LMG 19424 / R1)	31%	100%
B2SX63	Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)	30%	100%
B4RBS4	Phenylobacterium zucineum (strain HLK1)	34%	100%
B4U0U9	Streptococcus equi subsp. zooepidemicus (strain MGCS10565)	29%	100%
B5ZCB6	Ureaplasma urealyticum serovar 10 (strain ATCC 33699 / Western)	30%	100%
C0M8P2	Streptococcus equi subsp. equi (strain 4047)	29%	100%
C0MF36	Streptococcus equi subsp. zooepidemicus (strain H70)	29%	100%
C1AXY5	Rhodococcus opacus (strain B4)	28%	100%
C1CMT3	Streptococcus pneumoniae (strain P1031)	31%	100%
C3KXY4	Clostridium botulinum (strain 657 / Type Ba4)	32%	100%
C9ZQH0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	72%	98%
E9B0E5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
G0SEH7	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)	45%	97%
O22268	Arabidopsis thaliana	45%	100%
P41819	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	42%	100%
P78697	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	46%	100%
Q09522	Caenorhabditis elegans	46%	100%
Q0KEA7	Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337)	31%	100%
Q1J8J4	Streptococcus pyogenes serotype M4 (strain MGAS10750)	29%	100%
Q1JDL6	Streptococcus pyogenes serotype M12 (strain MGAS2096)	29%	100%
Q1JIN6	Streptococcus pyogenes serotype M2 (strain MGAS10270)	29%	100%
Q1JNI8	Streptococcus pyogenes serotype M12 (strain MGAS9429)	29%	100%
Q1WV73	Ligilactobacillus salivarius (strain UCC118)	32%	100%
Q223E6	Albidiferax ferrireducens (strain ATCC BAA-621 / DSM 15236 / T118)	29%	100%
Q2KHT8	Bos taurus	52%	100%
Q2NE42	Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)	37%	100%
Q38V22	Latilactobacillus sakei subsp. sakei (strain 23K)	30%	100%
Q475Q1	Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197)	32%	100%
Q48VC6	Streptococcus pyogenes serotype M28 (strain MGAS6180)	29%	100%
Q4Q7U7	Leishmania major	95%	100%
Q4QMZ9	Haemophilus influenzae (strain 86-028NP)	28%	100%
Q54QK7	Dictyostelium discoideum	50%	100%
Q5XDX4	Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)	29%	100%
Q65PH9	Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46)	31%	100%
Q6BSY5	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	44%	100%
Q6C7H6	Yarrowia lipolytica (strain CLIB 122 / E 150)	46%	100%
Q6FKY3	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	45%	100%
Q74C12	Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)	31%	100%
Q75C90	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	44%	100%
Q7VM33	Haemophilus ducreyi (strain 35000HP / ATCC 700724)	32%	100%
Q8P2N8	Streptococcus pyogenes serotype M18 (strain MGAS8232)	29%	100%
Q95KJ0	Macaca fascicularis	51%	100%
Q9A1I0	Streptococcus pyogenes serotype M1	29%	100%
Q9CHN8	Lactococcus lactis subsp. lactis (strain IL1403)	30%	100%
Q9CLL5	Pasteurella multocida (strain Pm70)	28%	100%
Q9D0D4	Mus musculus	51%	100%
Q9FK02	Arabidopsis thaliana	40%	98%
Q9PPN8	Ureaplasma parvum serovar 3 (strain ATCC 700970)	32%	100%
Q9UNQ2	Homo sapiens	51%	100%
Q9VAQ5	Drosophila melanogaster	50%	100%
V5BCC0	Trypanosoma cruzi	72%	100%