Cytochrome b5-like Heme/Steroid binding domain family protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cytochrome b5-like Heme/Steroid binding domain containing protein, putative

Species:

Leishmania donovani

UniProt:

A0A3S7X2E8_LEIDO

TriTrypDb:

LdBPK_290930.1 , LdCL_290015500 , LDHU3_29.1270

Length:

173

Annotations

LeishMANIAdb annotations

This cluster contains a high variety of cytochrome b5 homologs. Although all membrane-anchored, some use the N-terminal [type-III] while others the C-terminal TM helix [type-IV]. The heme domain is assumed to be cytoplasmic.. Localization: ER (by homology) / Mitochondrial outer membrane (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 25

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 16

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0016020	membrane	2	6
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	6
GO:0005635	nuclear envelope	4	1
GO:0005783	endoplasmic reticulum	5	1
GO:0020016	ciliary pocket	2	1
GO:0031967	organelle envelope	3	1
GO:0031975	envelope	2	1

Expansion

Sequence features

A0A3S7X2E8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7X2E8

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	3
GO:0020037	heme binding	4	3
GO:0046906	tetrapyrrole binding	3	3
GO:0097159	organic cyclic compound binding	2	3
GO:1901363	heterocyclic compound binding	2	3
GO:0043167	ion binding	2	1
GO:0043169	cation binding	3	1
GO:0046872	metal ion binding	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	78	82	PF00656	0.217
CLV_NRD_NRD_1	79	81	PF00675	0.275
CLV_PCSK_KEX2_1	59	61	PF00082	0.336
CLV_PCSK_KEX2_1	79	81	PF00082	0.271
CLV_PCSK_PC1ET2_1	59	61	PF00082	0.241
DEG_Nend_Nbox_1	1	3	PF02207	0.548
DOC_CKS1_1	168	173	PF01111	0.457
DOC_PP2B_LxvP_1	123	126	PF13499	0.497
DOC_USP7_MATH_1	146	150	PF00917	0.499
DOC_WW_Pin1_4	125	130	PF00397	0.526
DOC_WW_Pin1_4	167	172	PF00397	0.458
DOC_WW_Pin1_4	41	46	PF00397	0.241
DOC_WW_Pin1_4	88	93	PF00397	0.439
DOC_WW_Pin1_4	98	103	PF00397	0.655
LIG_14-3-3_CanoR_1	132	136	PF00244	0.539
LIG_14-3-3_CanoR_1	25	35	PF00244	0.333
LIG_FHA_1	1	7	PF00498	0.450
LIG_FHA_1	119	125	PF00498	0.534
LIG_FHA_1	154	160	PF00498	0.567
LIG_FHA_1	42	48	PF00498	0.331
LIG_FHA_2	113	119	PF00498	0.507
LIG_FHA_2	17	23	PF00498	0.241
LIG_FHA_2	76	82	PF00498	0.217
LIG_LIR_Gen_1	30	41	PF02991	0.213
LIG_LIR_LC3C_4	121	125	PF02991	0.505
LIG_LIR_Nem_3	30	36	PF02991	0.228
LIG_SH3_3	123	129	PF00018	0.528
LIG_SH3_3	165	171	PF00018	0.454
LIG_SUMO_SIM_par_1	39	44	PF11976	0.217
LIG_TRAF2_1	19	22	PF00917	0.326
MOD_CDK_SPxxK_3	125	132	PF00069	0.498
MOD_CK1_1	100	106	PF00069	0.746
MOD_CK1_1	116	122	PF00069	0.460
MOD_CK1_1	149	155	PF00069	0.471
MOD_CK2_1	112	118	PF00069	0.705
MOD_CK2_1	125	131	PF00069	0.615
MOD_CK2_1	134	140	PF00069	0.635
MOD_CK2_1	16	22	PF00069	0.326
MOD_GSK3_1	112	119	PF00069	0.505
MOD_GSK3_1	146	153	PF00069	0.511
MOD_GSK3_1	88	95	PF00069	0.399
MOD_GSK3_1	97	104	PF00069	0.551
MOD_N-GLC_1	26	31	PF02516	0.217
MOD_N-GLC_1	92	97	PF02516	0.387
MOD_PIKK_1	92	98	PF00454	0.533
MOD_PKA_2	131	137	PF00069	0.535
MOD_PKA_2	75	81	PF00069	0.217
MOD_Plk_4	146	152	PF00069	0.520
MOD_Plk_4	28	34	PF00069	0.286
MOD_Plk_4	36	42	PF00069	0.310
MOD_ProDKin_1	125	131	PF00069	0.527
MOD_ProDKin_1	167	173	PF00069	0.463
MOD_ProDKin_1	41	47	PF00069	0.241
MOD_ProDKin_1	88	94	PF00069	0.438
MOD_ProDKin_1	98	104	PF00069	0.658
TRG_DiLeu_BaLyEn_6	42	47	PF01217	0.217

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IB52	Leptomonas seymouri	58%	100%
A0A3S7WQZ7	Leishmania donovani	34%	100%
A0A422N6Z2	Trypanosoma rangeli	33%	76%
A4H5X6	Leishmania braziliensis	35%	100%
A4HU71	Leishmania infantum	34%	100%
E9ADU2	Leishmania major	43%	100%
E9ALY8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	43%	100%
E9AN01	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
Q4QHM6	Leishmania major	35%	100%