Adenine DNA glycosylase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

A/G-specific adenine glycosylase, putative

Species:

Leishmania donovani

UniProt:

A0A3S7X1P4_LEIDO

TriTrypDb:

LdBPK_282290.1 , LdCL_280028000 , LDHU3_28.3120

Length:

501

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A0A3S7X1P4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7X1P4

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006259	DNA metabolic process	4	12
GO:0006281	DNA repair	5	12
GO:0006284	base-excision repair	6	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0006950	response to stress	2	12
GO:0006974	DNA damage response	4	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0033554	cellular response to stress	3	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0046483	heterocycle metabolic process	3	12
GO:0050896	response to stimulus	1	12
GO:0051716	cellular response to stimulus	2	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006298	mismatch repair	6	1

Molecular functions
Term	Name	Level	Count
GO:0000700	mismatch base pair DNA N-glycosylase activity	5	12
GO:0000701	purine-specific mismatch base pair DNA N-glycosylase activity	6	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0016787	hydrolase activity	2	12
GO:0016798	hydrolase activity, acting on glycosyl bonds	3	12
GO:0016799	hydrolase activity, hydrolyzing N-glycosyl compounds	4	12
GO:0019104	DNA N-glycosylase activity	4	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0051536	iron-sulfur cluster binding	3	12
GO:0051539	4 iron, 4 sulfur cluster binding	4	12
GO:0051540	metal cluster binding	2	12
GO:0140097	catalytic activity, acting on DNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:0000702	oxidized base lesion DNA N-glycosylase activity	5	1
GO:0003676	nucleic acid binding	3	4
GO:0003677	DNA binding	4	4
GO:0003684	damaged DNA binding	5	1
GO:0003690	double-stranded DNA binding	5	1
GO:0008534	oxidized purine nucleobase lesion DNA N-glycosylase activity	6	1
GO:0030983	mismatched DNA binding	6	1
GO:0032356	oxidized DNA binding	6	1
GO:0032357	oxidized purine DNA binding	7	1
GO:0034039	8-oxo-7,8-dihydroguanine DNA N-glycosylase activity	7	1
GO:0035485	adenine/guanine mispair binding	7	1
GO:0097159	organic cyclic compound binding	2	4
GO:1901363	heterocyclic compound binding	2	4
GO:0004518	nuclease activity	4	2
GO:0004519	endonuclease activity	5	2
GO:0016788	hydrolase activity, acting on ester bonds	3	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	418	422	PF00656	0.305
CLV_NRD_NRD_1	134	136	PF00675	0.238
CLV_NRD_NRD_1	139	141	PF00675	0.242
CLV_NRD_NRD_1	306	308	PF00675	0.363
CLV_NRD_NRD_1	324	326	PF00675	0.293
CLV_NRD_NRD_1	330	332	PF00675	0.293
CLV_NRD_NRD_1	495	497	PF00675	0.683
CLV_PCSK_KEX2_1	134	136	PF00082	0.238
CLV_PCSK_KEX2_1	303	305	PF00082	0.523
CLV_PCSK_KEX2_1	324	326	PF00082	0.294
CLV_PCSK_KEX2_1	330	332	PF00082	0.294
CLV_PCSK_KEX2_1	414	416	PF00082	0.548
CLV_PCSK_KEX2_1	477	479	PF00082	0.573
CLV_PCSK_KEX2_1	491	493	PF00082	0.598
CLV_PCSK_KEX2_1	495	497	PF00082	0.616
CLV_PCSK_PC1ET2_1	303	305	PF00082	0.523
CLV_PCSK_PC1ET2_1	414	416	PF00082	0.458
CLV_PCSK_PC1ET2_1	477	479	PF00082	0.539
CLV_PCSK_PC1ET2_1	491	493	PF00082	0.523
CLV_PCSK_SKI1_1	141	145	PF00082	0.261
CLV_PCSK_SKI1_1	153	157	PF00082	0.328
CLV_PCSK_SKI1_1	297	301	PF00082	0.564
CLV_PCSK_SKI1_1	386	390	PF00082	0.307
CLV_PCSK_SKI1_1	437	441	PF00082	0.433
CLV_PCSK_SKI1_1	453	457	PF00082	0.273
DEG_APCC_DBOX_1	452	460	PF00400	0.381
DEG_SCF_FBW7_1	16	21	PF00400	0.458
DOC_CKS1_1	15	20	PF01111	0.501
DOC_CYCLIN_yCln2_LP_2	361	367	PF00134	0.295
DOC_MAPK_gen_1	303	311	PF00069	0.371
DOC_PP2B_LxvP_1	169	172	PF13499	0.528
DOC_PP4_FxxP_1	187	190	PF00568	0.445
DOC_SPAK_OSR1_1	325	329	PF12202	0.293
DOC_USP7_MATH_1	176	180	PF00917	0.448
DOC_USP7_MATH_1	18	22	PF00917	0.470
DOC_USP7_MATH_1	181	185	PF00917	0.427
DOC_USP7_MATH_1	190	194	PF00917	0.413
DOC_USP7_MATH_1	234	238	PF00917	0.571
DOC_USP7_MATH_1	302	306	PF00917	0.458
DOC_USP7_MATH_1	54	58	PF00917	0.595
DOC_USP7_UBL2_3	457	461	PF12436	0.346
DOC_WW_Pin1_4	14	19	PF00397	0.479
DOC_WW_Pin1_4	230	235	PF00397	0.504
DOC_WW_Pin1_4	485	490	PF00397	0.646
LIG_14-3-3_CanoR_1	386	396	PF00244	0.288
LIG_14-3-3_CanoR_1	50	54	PF00244	0.493
LIG_14-3-3_CanoR_1	70	80	PF00244	0.492
LIG_14-3-3_CterR_2	498	501	PF00244	0.767
LIG_BIR_II_1	1	5	PF00653	0.633
LIG_BIR_III_4	406	410	PF00653	0.295
LIG_BRCT_BRCA1_1	183	187	PF00533	0.418
LIG_deltaCOP1_diTrp_1	14	22	PF00928	0.406
LIG_FHA_1	234	240	PF00498	0.519
LIG_FHA_2	117	123	PF00498	0.529
LIG_FHA_2	91	97	PF00498	0.446
LIG_LIR_Apic_2	14	18	PF02991	0.497
LIG_LIR_Apic_2	184	190	PF02991	0.499
LIG_LIR_Nem_3	26	31	PF02991	0.378
LIG_LIR_Nem_3	342	346	PF02991	0.295
LIG_LIR_Nem_3	75	81	PF02991	0.488
LIG_LRP6_Inhibitor_1	194	200	PF00058	0.319
LIG_PCNA_yPIPBox_3	29	39	PF02747	0.382
LIG_PCNA_yPIPBox_3	330	341	PF02747	0.295
LIG_Pex14_1	105	109	PF04695	0.418
LIG_RPA_C_Fungi	319	331	PF08784	0.321
LIG_SH2_CRK	28	32	PF00017	0.363
LIG_SH2_NCK_1	177	181	PF00017	0.279
LIG_SH2_STAP1	102	106	PF00017	0.227
LIG_SH2_STAP1	177	181	PF00017	0.273
LIG_SH2_STAT5	138	141	PF00017	0.256
LIG_SH2_STAT5	145	148	PF00017	0.261
LIG_SH2_STAT5	177	180	PF00017	0.268
LIG_SH3_3	210	216	PF00018	0.295
LIG_SH3_3	255	261	PF00018	0.332
LIG_SH3_3	326	332	PF00018	0.318
LIG_SH3_3	398	404	PF00018	0.390
LIG_SUMO_SIM_anti_2	395	400	PF11976	0.281
LIG_SUMO_SIM_par_1	363	369	PF11976	0.281
LIG_TRAF2_1	163	166	PF00917	0.398
LIG_TRAF2_1	30	33	PF00917	0.397
LIG_TRAF2_1	479	482	PF00917	0.636
LIG_UBA3_1	455	461	PF00899	0.401
LIG_WRC_WIRS_1	19	24	PF05994	0.410
MOD_CDC14_SPxK_1	488	491	PF00782	0.642
MOD_CDK_SPxK_1	485	491	PF00069	0.647
MOD_CDK_SPxxK_3	485	492	PF00069	0.650
MOD_CK1_1	2	8	PF00069	0.694
MOD_CK1_1	21	27	PF00069	0.443
MOD_CK1_1	233	239	PF00069	0.578
MOD_CK1_1	366	372	PF00069	0.281
MOD_CK1_1	72	78	PF00069	0.495
MOD_CK1_1	90	96	PF00069	0.258
MOD_CK2_1	116	122	PF00069	0.361
MOD_CK2_1	160	166	PF00069	0.321
MOD_CK2_1	27	33	PF00069	0.458
MOD_CK2_1	460	466	PF00069	0.491
MOD_CK2_1	90	96	PF00069	0.359
MOD_Cter_Amidation	475	478	PF01082	0.659
MOD_GlcNHglycan	178	181	PF01048	0.352
MOD_GlcNHglycan	236	239	PF01048	0.533
MOD_GlcNHglycan	243	247	PF01048	0.400
MOD_GlcNHglycan	261	264	PF01048	0.263
MOD_GlcNHglycan	278	281	PF01048	0.444
MOD_GlcNHglycan	304	307	PF01048	0.487
MOD_GlcNHglycan	314	318	PF01048	0.412
MOD_GlcNHglycan	377	380	PF01048	0.375
MOD_GlcNHglycan	4	7	PF01048	0.688
MOD_GlcNHglycan	423	426	PF01048	0.584
MOD_GlcNHglycan	56	59	PF01048	0.699
MOD_GlcNHglycan	60	63	PF01048	0.709
MOD_GlcNHglycan	71	74	PF01048	0.669
MOD_GSK3_1	14	21	PF00069	0.560
MOD_GSK3_1	230	237	PF00069	0.497
MOD_GSK3_1	481	488	PF00069	0.650
MOD_GSK3_1	54	61	PF00069	0.567
MOD_GSK3_1	68	75	PF00069	0.628
MOD_GSK3_1	83	90	PF00069	0.228
MOD_NEK2_1	254	259	PF00069	0.496
MOD_NEK2_1	266	271	PF00069	0.381
MOD_NEK2_1	315	320	PF00069	0.408
MOD_NEK2_2	7	12	PF00069	0.469
MOD_PIKK_1	161	167	PF00454	0.362
MOD_PIKK_1	87	93	PF00454	0.258
MOD_PK_1	108	114	PF00069	0.410
MOD_PK_1	461	467	PF00069	0.397
MOD_PKA_2	2	8	PF00069	0.568
MOD_PKA_2	259	265	PF00069	0.393
MOD_PKA_2	374	380	PF00069	0.348
MOD_PKA_2	470	476	PF00069	0.582
MOD_PKA_2	49	55	PF00069	0.495
MOD_PKA_2	69	75	PF00069	0.583
MOD_PKB_1	373	381	PF00069	0.200
MOD_Plk_4	122	128	PF00069	0.401
MOD_Plk_4	363	369	PF00069	0.348
MOD_Plk_4	94	100	PF00069	0.258
MOD_ProDKin_1	14	20	PF00069	0.484
MOD_ProDKin_1	230	236	PF00069	0.499
MOD_ProDKin_1	485	491	PF00069	0.647
MOD_SUMO_rev_2	118	126	PF00179	0.401
MOD_SUMO_rev_2	208	216	PF00179	0.257
MOD_SUMO_rev_2	463	470	PF00179	0.489
TRG_DiLeu_BaLyEn_6	356	361	PF01217	0.295
TRG_ENDOCYTIC_2	28	31	PF00928	0.367
TRG_ER_diArg_1	133	135	PF00400	0.258
TRG_ER_diArg_1	324	326	PF00400	0.294
TRG_ER_diArg_1	329	331	PF00400	0.294
TRG_ER_diArg_1	373	376	PF00400	0.295
TRG_Pf-PMV_PEXEL_1	144	148	PF00026	0.415
TRG_Pf-PMV_PEXEL_1	478	482	PF00026	0.578

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IL90	Leptomonas seymouri	61%	98%
A0A0S4IRI2	Bodo saltans	37%	95%
A0A1X0NR87	Trypanosomatidae	45%	100%
A0A422N7D8	Trypanosoma rangeli	42%	100%
A4HGR8	Leishmania braziliensis	82%	100%
A4I3U3	Leishmania infantum	100%	100%
D0A913	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
E9B035	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
F4JRF4	Arabidopsis thaliana	34%	80%
O31584	Bacillus subtilis (strain 168)	29%	100%
P17802	Escherichia coli (strain K12)	30%	100%
P44320	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)	28%	100%
P57617	Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)	25%	100%
P83847	Geobacillus stearothermophilus	33%	100%
Q05869	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	29%	100%
Q10159	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	32%	100%
Q4Q851	Leishmania major	94%	100%
Q8R5G2	Rattus norvegicus	30%	97%
Q99P21	Mus musculus	31%	97%
Q9UIF7	Homo sapiens	32%	92%
V5B3S1	Trypanosoma cruzi	42%	100%