Protein_disulfide_isomerase_putative/GeneDB:LmjF. 26.0660

Quick info Annotations Localization Abundance Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

protein disulfide isomerase, putative

Species:

Leishmania donovani

UniProt:

A0A3S7WZP2_LEIDO

TriTrypDb:

LdBPK_260630.1 , LdCL_260012000 , LDHU3_26.0840

Length:

377

Annotations

LeishMANIAdb annotations

Appears to be a family of secreted proteins, probably ER-localized, like its homologs in other Eukaryotes.. Seems to carry a putative KDEL-like signal on its C-terminus.. Localization: Secreted (by homology)

Annotations by Jardim et al.

Redox homeostasis, Thioredoxin Uncharacterized

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	yes	yes: 4
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 16

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 9, no: 2
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005783	endoplasmic reticulum	5	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12
GO:0005794	Golgi apparatus	5	1

Abundance

Amastigote (protein)


Source	Evidence on protein	Close homologs
Pescher et al. (upgregulation)	no	yes: 0

Promastigote and amastigote


Source	Evidence on protein	Close homologs
Lahav et al. - mRNA - Protein

Expansion

Sequence features

A0A3S7WZP2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7WZP2

Function

Biological processes
Term	Name	Level	Count
GO:0006457	protein folding	2	1
GO:0009987	cellular process	1	1

Molecular functions
Term	Name	Level	Count
GO:0003756	protein disulfide isomerase activity	3	10
GO:0003824	catalytic activity	1	11
GO:0016853	isomerase activity	2	11
GO:0016860	intramolecular oxidoreductase activity	3	10
GO:0016864	intramolecular oxidoreductase activity, transposing S-S bonds	4	10
GO:0140096	catalytic activity, acting on a protein	2	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	357	361	PF00656	0.322
CLV_MEL_PAP_1	101	107	PF00089	0.504
CLV_NRD_NRD_1	220	222	PF00675	0.468
CLV_NRD_NRD_1	3	5	PF00675	0.300
CLV_NRD_NRD_1	358	360	PF00675	0.504
CLV_PCSK_FUR_1	345	349	PF00082	0.468
CLV_PCSK_KEX2_1	103	105	PF00082	0.565
CLV_PCSK_KEX2_1	3	5	PF00082	0.297
CLV_PCSK_KEX2_1	347	349	PF00082	0.499
CLV_PCSK_KEX2_1	358	360	PF00082	0.427
CLV_PCSK_PC1ET2_1	103	105	PF00082	0.468
CLV_PCSK_PC1ET2_1	347	349	PF00082	0.529
CLV_PCSK_SKI1_1	174	178	PF00082	0.486
CLV_PCSK_SKI1_1	189	193	PF00082	0.453
CLV_PCSK_SKI1_1	197	201	PF00082	0.495
CLV_PCSK_SKI1_1	222	226	PF00082	0.499
CLV_PCSK_SKI1_1	292	296	PF00082	0.519
CLV_PCSK_SKI1_1	50	54	PF00082	0.572
CLV_PCSK_SKI1_1	85	89	PF00082	0.514
DEG_Nend_UBRbox_3	1	3	PF02207	0.609
DEG_SPOP_SBC_1	293	297	PF00917	0.267
DOC_CYCLIN_yClb5_NLxxxL_5	253	259	PF00134	0.268
DOC_MAPK_gen_1	3	11	PF00069	0.497
DOC_MAPK_gen_1	358	366	PF00069	0.237
DOC_MAPK_gen_1	48	56	PF00069	0.300
DOC_MAPK_MEF2A_6	3	11	PF00069	0.492
DOC_MAPK_MEF2A_6	359	368	PF00069	0.323
DOC_PP2B_LxvP_1	148	151	PF13499	0.328
DOC_PP4_FxxP_1	180	183	PF00568	0.253
DOC_PP4_FxxP_1	56	59	PF00568	0.253
DOC_SPAK_OSR1_1	104	108	PF12202	0.249
DOC_USP7_MATH_1	117	121	PF00917	0.214
DOC_USP7_MATH_1	294	298	PF00917	0.323
DOC_USP7_MATH_1	310	314	PF00917	0.344
DOC_USP7_UBL2_3	170	174	PF12436	0.344
LIG_14-3-3_CanoR_1	221	229	PF00244	0.268
LIG_14-3-3_CanoR_1	3	8	PF00244	0.484
LIG_14-3-3_CanoR_1	345	351	PF00244	0.300
LIG_Actin_WH2_2	132	149	PF00022	0.319
LIG_FHA_2	129	135	PF00498	0.268
LIG_FHA_2	161	167	PF00498	0.379
LIG_FHA_2	312	318	PF00498	0.352
LIG_Integrin_isoDGR_2	251	253	PF01839	0.468
LIG_LIR_Apic_2	234	239	PF02991	0.300
LIG_LIR_Apic_2	55	59	PF02991	0.249
LIG_LIR_Gen_1	163	172	PF02991	0.262
LIG_LIR_Gen_1	256	266	PF02991	0.375
LIG_LIR_Gen_1	40	46	PF02991	0.357
LIG_LIR_Gen_1	70	78	PF02991	0.313
LIG_LIR_Nem_3	108	113	PF02991	0.311
LIG_LIR_Nem_3	133	138	PF02991	0.263
LIG_LIR_Nem_3	163	168	PF02991	0.262
LIG_LIR_Nem_3	230	235	PF02991	0.217
LIG_LIR_Nem_3	256	261	PF02991	0.375
LIG_LIR_Nem_3	277	283	PF02991	0.395
LIG_LIR_Nem_3	40	45	PF02991	0.357
LIG_LIR_Nem_3	70	74	PF02991	0.299
LIG_Pex14_2	180	184	PF04695	0.255
LIG_Pex14_2	56	60	PF04695	0.239
LIG_RPA_C_Fungi	207	219	PF08784	0.309
LIG_SH2_CRK	195	199	PF00017	0.457
LIG_SH2_CRK	280	284	PF00017	0.284
LIG_SH2_CRK	71	75	PF00017	0.324
LIG_SH2_SRC	338	341	PF00017	0.287
LIG_SH2_STAT5	115	118	PF00017	0.363
LIG_SH2_STAT5	138	141	PF00017	0.464
LIG_SH2_STAT5	236	239	PF00017	0.448
LIG_SH2_STAT5	324	327	PF00017	0.377
LIG_SH2_STAT5	338	341	PF00017	0.365
LIG_SH3_3	148	154	PF00018	0.624
LIG_SH3_3	166	172	PF00018	0.372
LIG_SUMO_SIM_anti_2	86	91	PF11976	0.459
LIG_UBA3_1	368	377	PF00899	0.405
LIG_UBA3_1	87	92	PF00899	0.453
LIG_WRC_WIRS_1	258	263	PF05994	0.415
MOD_CK1_1	21	27	PF00069	0.550
MOD_CK1_1	297	303	PF00069	0.457
MOD_CK1_1	313	319	PF00069	0.391
MOD_CK2_1	293	299	PF00069	0.338
MOD_CK2_1	311	317	PF00069	0.500
MOD_Cter_Amidation	101	104	PF01082	0.309
MOD_GlcNHglycan	117	120	PF01048	0.348
MOD_GlcNHglycan	140	143	PF01048	0.453
MOD_GlcNHglycan	229	232	PF01048	0.378
MOD_GlcNHglycan	296	299	PF01048	0.384
MOD_GlcNHglycan	342	345	PF01048	0.381
MOD_GSK3_1	105	112	PF00069	0.343
MOD_GSK3_1	227	234	PF00069	0.324
MOD_GSK3_1	293	300	PF00069	0.400
MOD_GSK3_1	311	318	PF00069	0.409
MOD_NEK2_1	105	110	PF00069	0.359
MOD_NEK2_1	146	151	PF00069	0.536
MOD_NEK2_1	160	165	PF00069	0.260
MOD_NEK2_1	18	23	PF00069	0.378
MOD_NEK2_1	346	351	PF00069	0.395
MOD_PK_1	3	9	PF00069	0.353
MOD_PKA_1	3	9	PF00069	0.419
MOD_PKA_2	146	152	PF00069	0.529
MOD_PKA_2	3	9	PF00069	0.320
MOD_Plk_1	274	280	PF00069	0.385
MOD_Plk_4	109	115	PF00069	0.312
MOD_Plk_4	231	237	PF00069	0.322
MOD_Plk_4	257	263	PF00069	0.328
MOD_Plk_4	3	9	PF00069	0.335
TRG_ENDOCYTIC_2	195	198	PF00928	0.373
TRG_ENDOCYTIC_2	280	283	PF00928	0.434
TRG_ENDOCYTIC_2	71	74	PF00928	0.347
TRG_ER_diArg_1	3	5	PF00400	0.411
TRG_Pf-PMV_PEXEL_1	253	257	PF00026	0.299

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HVU7	Leptomonas seymouri	50%	100%
A0A0S4IKM8	Bodo saltans	23%	98%
A0A0S4JJE1	Bodo saltans	47%	100%
A0A1X0NSF7	Trypanosomatidae	48%	99%
A0A422N2J8	Trypanosoma rangeli	49%	100%
A4HES1	Leishmania braziliensis	53%	100%
A4I1Z8	Leishmania infantum	100%	100%
C9ZS16	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	100%
E9AY45	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
O13704	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	25%	100%
O13811	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	37%	100%
O22263	Arabidopsis thaliana	43%	100%
P38660	Mesocricetus auratus	33%	86%
P38661	Medicago sativa	42%	100%
Q00216	Aspergillus niger	42%	100%
Q11067	Caenorhabditis elegans	30%	86%
Q15084	Homo sapiens	34%	86%
Q4Q9C8	Leishmania major	94%	100%
Q5I0H9	Rattus norvegicus	33%	73%
Q5R6T1	Pongo abelii	34%	86%
Q63081	Rattus norvegicus	33%	86%
Q75M08	Oryza sativa subsp. japonica	43%	100%
Q86IA3	Dictyostelium discoideum	35%	100%
Q92249	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	41%	100%
Q922R8	Mus musculus	33%	86%
Q942L2	Oryza sativa subsp. japonica	40%	100%
Q9MAU6	Arabidopsis thaliana	30%	84%
Q9V438	Drosophila melanogaster	37%	87%
Q9VYV3	Drosophila melanogaster	27%	91%
V5DR98	Trypanosoma cruzi	45%	100%