Diphthine--ammonia ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

MJ0570-related uncharacterized domain containing protein, putative

Species:

Leishmania donovani

UniProt:

A0A3S7WYW4_LEIDO

TriTrypDb:

LdBPK_250300.1 , LdCL_250008100 , LDHU3_25.0370

Length:

615

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

A0A3S7WYW4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7WYW4

Function

Biological processes
Term	Name	Level	Count
GO:0006417	regulation of translation	6	1
GO:0006448	regulation of translational elongation	7	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009058	biosynthetic process	2	1
GO:0009889	regulation of biosynthetic process	4	1
GO:0009987	cellular process	1	1
GO:0010468	regulation of gene expression	5	1
GO:0010556	regulation of macromolecule biosynthetic process	5	1
GO:0010608	post-transcriptional regulation of gene expression	6	1
GO:0017182	peptidyl-diphthamide metabolic process	7	1
GO:0017183	peptidyl-diphthamide biosynthetic process from peptidyl-histidine	4	1
GO:0018193	peptidyl-amino acid modification	5	1
GO:0018202	peptidyl-histidine modification	6	1
GO:0019222	regulation of metabolic process	3	1
GO:0019538	protein metabolic process	3	1
GO:0031323	regulation of cellular metabolic process	4	1
GO:0031326	regulation of cellular biosynthetic process	5	1
GO:0034248	regulation of amide metabolic process	5	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0051171	regulation of nitrogen compound metabolic process	4	1
GO:0051246	regulation of protein metabolic process	5	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0065007	biological regulation	1	1
GO:0071704	organic substance metabolic process	2	1
GO:0080090	regulation of primary metabolic process	4	1
GO:1900247	regulation of cytoplasmic translational elongation	8	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:2000112	obsolete regulation of cellular macromolecule biosynthetic process	6	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	5
GO:0003824	catalytic activity	1	10
GO:0005488	binding	1	5
GO:0005524	ATP binding	5	5
GO:0016874	ligase activity	2	10
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	10
GO:0016880	acid-ammonia (or amide) ligase activity	4	10
GO:0017076	purine nucleotide binding	4	5
GO:0017178	diphthine-ammonia ligase activity	5	10
GO:0030554	adenyl nucleotide binding	5	5
GO:0032553	ribonucleotide binding	3	5
GO:0032555	purine ribonucleotide binding	4	5
GO:0032559	adenyl ribonucleotide binding	5	5
GO:0035639	purine ribonucleoside triphosphate binding	4	5
GO:0036094	small molecule binding	2	5
GO:0043167	ion binding	2	5
GO:0043168	anion binding	3	5
GO:0097159	organic cyclic compound binding	2	5
GO:0097367	carbohydrate derivative binding	2	5
GO:1901265	nucleoside phosphate binding	3	5
GO:1901363	heterocyclic compound binding	2	5

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	67	71	PF00656	0.368
CLV_NRD_NRD_1	188	190	PF00675	0.370
CLV_NRD_NRD_1	81	83	PF00675	0.314
CLV_NRD_NRD_1	86	88	PF00675	0.314
CLV_PCSK_FUR_1	84	88	PF00082	0.339
CLV_PCSK_KEX2_1	188	190	PF00082	0.281
CLV_PCSK_KEX2_1	81	83	PF00082	0.314
CLV_PCSK_KEX2_1	86	88	PF00082	0.314
CLV_PCSK_PC7_1	82	88	PF00082	0.414
CLV_PCSK_SKI1_1	115	119	PF00082	0.300
CLV_PCSK_SKI1_1	174	178	PF00082	0.296
CLV_PCSK_SKI1_1	327	331	PF00082	0.596
CLV_PCSK_SKI1_1	376	380	PF00082	0.394
CLV_PCSK_SKI1_1	536	540	PF00082	0.426
DEG_APCC_DBOX_1	146	154	PF00400	0.339
DEG_APCC_DBOX_1	198	206	PF00400	0.371
DEG_Nend_UBRbox_1	1	4	PF02207	0.364
DOC_CYCLIN_RxL_1	533	544	PF00134	0.457
DOC_CYCLIN_yCln2_LP_2	225	231	PF00134	0.414
DOC_MAPK_gen_1	279	287	PF00069	0.491
DOC_MAPK_MEF2A_6	327	334	PF00069	0.415
DOC_MAPK_NFAT4_5	327	335	PF00069	0.419
DOC_PP1_RVXF_1	138	144	PF00149	0.369
DOC_PP2B_LxvP_1	432	435	PF13499	0.396
DOC_PP2B_LxvP_1	76	79	PF13499	0.414
DOC_PP4_FxxP_1	385	388	PF00568	0.500
DOC_USP7_MATH_1	311	315	PF00917	0.719
DOC_USP7_MATH_1	388	392	PF00917	0.485
DOC_USP7_MATH_1	402	406	PF00917	0.416
DOC_USP7_MATH_1	611	615	PF00917	0.675
DOC_USP7_MATH_1	68	72	PF00917	0.334
DOC_WW_Pin1_4	183	188	PF00397	0.242
DOC_WW_Pin1_4	248	253	PF00397	0.434
DOC_WW_Pin1_4	307	312	PF00397	0.487
DOC_WW_Pin1_4	376	381	PF00397	0.444
DOC_WW_Pin1_4	477	482	PF00397	0.502
LIG_14-3-3_CanoR_1	464	469	PF00244	0.520
LIG_14-3-3_CanoR_1	474	484	PF00244	0.481
LIG_14-3-3_CanoR_1	536	541	PF00244	0.457
LIG_14-3-3_CanoR_1	599	603	PF00244	0.505
LIG_Actin_WH2_2	65	83	PF00022	0.339
LIG_BIR_III_2	290	294	PF00653	0.349
LIG_BRCT_BRCA1_1	327	331	PF00533	0.615
LIG_deltaCOP1_diTrp_1	389	395	PF00928	0.327
LIG_DLG_GKlike_1	464	472	PF00625	0.331
LIG_EVH1_1	320	324	PF00568	0.485
LIG_FHA_1	1	7	PF00498	0.356
LIG_FHA_1	168	174	PF00498	0.321
LIG_FHA_1	178	184	PF00498	0.309
LIG_FHA_1	220	226	PF00498	0.314
LIG_FHA_1	230	236	PF00498	0.282
LIG_FHA_1	422	428	PF00498	0.598
LIG_FHA_1	434	440	PF00498	0.390
LIG_FHA_1	468	474	PF00498	0.543
LIG_FHA_1	485	491	PF00498	0.387
LIG_FHA_1	519	525	PF00498	0.374
LIG_FHA_1	554	560	PF00498	0.377
LIG_FHA_1	573	579	PF00498	0.402
LIG_FHA_1	593	599	PF00498	0.450
LIG_FHA_2	114	120	PF00498	0.399
LIG_FHA_2	192	198	PF00498	0.414
LIG_FHA_2	490	496	PF00498	0.441
LIG_LIR_Apic_2	317	323	PF02991	0.502
LIG_LIR_Apic_2	347	351	PF02991	0.504
LIG_LIR_Gen_1	108	117	PF02991	0.378
LIG_LIR_Gen_1	152	161	PF02991	0.414
LIG_LIR_Gen_1	535	545	PF02991	0.503
LIG_LIR_Gen_1	58	69	PF02991	0.314
LIG_LIR_Nem_3	108	114	PF02991	0.314
LIG_LIR_Nem_3	119	124	PF02991	0.314
LIG_LIR_Nem_3	328	334	PF02991	0.522
LIG_LIR_Nem_3	379	385	PF02991	0.377
LIG_LIR_Nem_3	535	540	PF02991	0.468
LIG_LIR_Nem_3	544	548	PF02991	0.403
LIG_LIR_Nem_3	58	64	PF02991	0.314
LIG_PDZ_Class_3	610	615	PF00595	0.589
LIG_Pex14_2	381	385	PF04695	0.396
LIG_Pex14_2	541	545	PF04695	0.507
LIG_SH2_CRK	111	115	PF00017	0.339
LIG_SH2_CRK	348	352	PF00017	0.519
LIG_SH2_CRK	61	65	PF00017	0.414
LIG_SH2_STAP1	220	224	PF00017	0.414
LIG_SH2_STAP1	400	404	PF00017	0.455
LIG_SH2_STAT3	59	62	PF00017	0.313
LIG_SH2_STAT3	97	100	PF00017	0.350
LIG_SH2_STAT5	155	158	PF00017	0.414
LIG_SH2_STAT5	503	506	PF00017	0.359
LIG_SH2_STAT5	537	540	PF00017	0.427
LIG_SH3_3	260	266	PF00018	0.525
LIG_SH3_3	282	288	PF00018	0.438
LIG_SH3_3	299	305	PF00018	0.332
LIG_SH3_3	318	324	PF00018	0.404
LIG_SH3_4	265	272	PF00018	0.459
LIG_SUMO_SIM_anti_2	3	8	PF11976	0.347
LIG_SUMO_SIM_par_1	2	8	PF11976	0.354
LIG_SUMO_SIM_par_1	464	470	PF11976	0.247
LIG_TRAF2_1	101	104	PF00917	0.456
LIG_TRAF2_1	194	197	PF00917	0.371
LIG_TRAF2_1	610	613	PF00917	0.665
LIG_TRFH_1	301	305	PF08558	0.430
MOD_CDK_SPK_2	183	188	PF00069	0.242
MOD_CDK_SPxK_1	183	189	PF00069	0.242
MOD_CDK_SPxK_1	477	483	PF00069	0.505
MOD_CK1_1	109	115	PF00069	0.378
MOD_CK1_1	314	320	PF00069	0.688
MOD_CK1_1	421	427	PF00069	0.488
MOD_CK1_1	467	473	PF00069	0.344
MOD_CK2_1	113	119	PF00069	0.302
MOD_CK2_1	191	197	PF00069	0.369
MOD_CK2_1	383	389	PF00069	0.503
MOD_CK2_1	590	596	PF00069	0.489
MOD_CMANNOS	392	395	PF00535	0.318
MOD_DYRK1A_RPxSP_1	376	380	PF00069	0.446
MOD_GlcNHglycan	128	131	PF01048	0.371
MOD_GlcNHglycan	252	255	PF01048	0.412
MOD_GlcNHglycan	305	308	PF01048	0.600
MOD_GlcNHglycan	313	316	PF01048	0.648
MOD_GlcNHglycan	327	330	PF01048	0.470
MOD_GlcNHglycan	477	480	PF01048	0.371
MOD_GlcNHglycan	504	507	PF01048	0.370
MOD_GlcNHglycan	514	519	PF01048	0.450
MOD_GlcNHglycan	592	595	PF01048	0.470
MOD_GlcNHglycan	7	10	PF01048	0.342
MOD_GSK3_1	109	116	PF00069	0.339
MOD_GSK3_1	179	186	PF00069	0.242
MOD_GSK3_1	229	236	PF00069	0.375
MOD_GSK3_1	303	310	PF00069	0.611
MOD_GSK3_1	364	371	PF00069	0.517
MOD_GSK3_1	412	419	PF00069	0.509
MOD_GSK3_1	514	521	PF00069	0.570
MOD_GSK3_1	568	575	PF00069	0.457
MOD_GSK3_1	91	98	PF00069	0.375
MOD_N-GLC_1	48	53	PF02516	0.579
MOD_N-GLC_2	363	365	PF02516	0.536
MOD_N-GLC_2	418	420	PF02516	0.402
MOD_NEK2_1	113	118	PF00069	0.377
MOD_NEK2_1	167	172	PF00069	0.340
MOD_NEK2_1	177	182	PF00069	0.297
MOD_NEK2_1	208	213	PF00069	0.335
MOD_NEK2_1	294	299	PF00069	0.479
MOD_NEK2_1	374	379	PF00069	0.519
MOD_NEK2_1	412	417	PF00069	0.464
MOD_NEK2_1	475	480	PF00069	0.463
MOD_NEK2_1	507	512	PF00069	0.490
MOD_NEK2_1	541	546	PF00069	0.490
MOD_NEK2_1	60	65	PF00069	0.414
MOD_NEK2_2	469	474	PF00069	0.345
MOD_PIKK_1	458	464	PF00454	0.514
MOD_PIKK_1	507	513	PF00454	0.537
MOD_PIKK_1	569	575	PF00454	0.533
MOD_PK_1	179	185	PF00069	0.339
MOD_PKA_2	198	204	PF00069	0.414
MOD_PKA_2	421	427	PF00069	0.565
MOD_PKA_2	598	604	PF00069	0.513
MOD_Plk_1	220	226	PF00069	0.394
MOD_Plk_1	374	380	PF00069	0.454
MOD_Plk_1	91	97	PF00069	0.375
MOD_Plk_4	10	16	PF00069	0.338
MOD_Plk_4	106	112	PF00069	0.282
MOD_Plk_4	220	226	PF00069	0.309
MOD_Plk_4	364	370	PF00069	0.460
MOD_Plk_4	536	542	PF00069	0.378
MOD_Plk_4	55	61	PF00069	0.414
MOD_Plk_4	91	97	PF00069	0.402
MOD_ProDKin_1	183	189	PF00069	0.242
MOD_ProDKin_1	248	254	PF00069	0.426
MOD_ProDKin_1	307	313	PF00069	0.493
MOD_ProDKin_1	376	382	PF00069	0.440
MOD_ProDKin_1	477	483	PF00069	0.505
MOD_SUMO_for_1	117	120	PF00179	0.304
TRG_DiLeu_BaLyEn_6	290	295	PF01217	0.479
TRG_ENDOCYTIC_2	111	114	PF00928	0.339
TRG_ENDOCYTIC_2	155	158	PF00928	0.414
TRG_ENDOCYTIC_2	382	385	PF00928	0.486
TRG_ENDOCYTIC_2	537	540	PF00928	0.427
TRG_ENDOCYTIC_2	61	64	PF00928	0.414
TRG_ER_diArg_1	138	141	PF00400	0.339
TRG_ER_diArg_1	187	189	PF00400	0.281
TRG_ER_diArg_1	80	82	PF00400	0.314
TRG_ER_diArg_1	84	87	PF00400	0.314
TRG_NES_CRM1_1	596	609	PF08389	0.496
TRG_Pf-PMV_PEXEL_1	193	197	PF00026	0.414
TRG_Pf-PMV_PEXEL_1	87	92	PF00026	0.378

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PEV4	Leptomonas seymouri	59%	100%
A0A1X0P4I2	Trypanosomatidae	39%	100%
A0A3R7K7F7	Trypanosoma rangeli	41%	100%
A4HDX7	Leishmania braziliensis	79%	100%
A4I172	Leishmania infantum	100%	100%
D0A5F8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	38%	100%
E9AXA6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
Q4QA66	Leishmania major	91%	100%
Q9USQ7	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	100%