Vps54_N domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Protein of unknown function N-terminal domain (DUF2450)/Protein of unknown function C-terminus (DUF2451), putative

Species:

Leishmania donovani

UniProt:

A0A3S7WYM4_LEIDO

TriTrypDb:

LdBPK_242260.1 , LdCL_240028200 , LDHU3_24.2780

Length:

1016

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005829	cytosol	2	11
GO:0032991	protein-containing complex	1	11
GO:0110165	cellular anatomical entity	1	11
GO:1990745	EARP complex	2	11

Expansion

Sequence features

A0A3S7WYM4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7WYM4

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	11
GO:0009987	cellular process	1	11
GO:0016192	vesicle-mediated transport	4	11
GO:0016197	endosomal transport	4	11
GO:0016482	cytosolic transport	4	11
GO:0032456	endocytic recycling	5	11
GO:0042147	retrograde transport, endosome to Golgi	5	11
GO:0046907	intracellular transport	3	11
GO:0051179	localization	1	11
GO:0051234	establishment of localization	2	11
GO:0051641	cellular localization	2	11
GO:0051649	establishment of localization in cell	3	11
GO:0051668	localization within membrane	3	11
GO:0098876	vesicle-mediated transport to the plasma membrane	4	11

Molecular functions
Term	Name	Level	Count
GO:0000149	SNARE binding	3	1
GO:0005488	binding	1	1
GO:0005515	protein binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	115	119	PF00656	0.354
CLV_C14_Caspase3-7	174	178	PF00656	0.416
CLV_C14_Caspase3-7	595	599	PF00656	0.607
CLV_C14_Caspase3-7	684	688	PF00656	0.361
CLV_NRD_NRD_1	156	158	PF00675	0.328
CLV_NRD_NRD_1	193	195	PF00675	0.319
CLV_NRD_NRD_1	23	25	PF00675	0.689
CLV_NRD_NRD_1	419	421	PF00675	0.543
CLV_NRD_NRD_1	54	56	PF00675	0.561
CLV_NRD_NRD_1	737	739	PF00675	0.435
CLV_NRD_NRD_1	877	879	PF00675	0.368
CLV_PCSK_KEX2_1	155	157	PF00082	0.279
CLV_PCSK_KEX2_1	232	234	PF00082	0.379
CLV_PCSK_KEX2_1	253	255	PF00082	0.556
CLV_PCSK_KEX2_1	54	56	PF00082	0.538
CLV_PCSK_KEX2_1	737	739	PF00082	0.435
CLV_PCSK_KEX2_1	877	879	PF00082	0.252
CLV_PCSK_KEX2_1	912	914	PF00082	0.427
CLV_PCSK_PC1ET2_1	155	157	PF00082	0.279
CLV_PCSK_PC1ET2_1	232	234	PF00082	0.379
CLV_PCSK_PC1ET2_1	253	255	PF00082	0.556
CLV_PCSK_PC1ET2_1	912	914	PF00082	0.427
CLV_PCSK_SKI1_1	100	104	PF00082	0.345
CLV_PCSK_SKI1_1	156	160	PF00082	0.301
CLV_PCSK_SKI1_1	27	31	PF00082	0.555
CLV_PCSK_SKI1_1	285	289	PF00082	0.492
CLV_PCSK_SKI1_1	309	313	PF00082	0.407
CLV_PCSK_SKI1_1	342	346	PF00082	0.497
CLV_PCSK_SKI1_1	479	483	PF00082	0.406
CLV_PCSK_SKI1_1	533	537	PF00082	0.384
CLV_PCSK_SKI1_1	54	58	PF00082	0.537
CLV_PCSK_SKI1_1	893	897	PF00082	0.326
CLV_PCSK_SKI1_1	950	954	PF00082	0.369
DEG_APCC_DBOX_1	232	240	PF00400	0.320
DEG_APCC_KENBOX_2	212	216	PF00400	0.427
DEG_COP1_1	940	947	PF00400	0.349
DEG_Nend_UBRbox_2	1	3	PF02207	0.563
DEG_SCF_FBW7_2	880	887	PF00400	0.347
DEG_SPOP_SBC_1	569	573	PF00917	0.600
DEG_SPOP_SBC_1	640	644	PF00917	0.487
DOC_ANK_TNKS_1	132	139	PF00023	0.416
DOC_CKS1_1	490	495	PF01111	0.481
DOC_CKS1_1	729	734	PF01111	0.547
DOC_CKS1_1	881	886	PF01111	0.345
DOC_MAPK_FxFP_2	753	756	PF00069	0.440
DOC_MAPK_gen_1	232	239	PF00069	0.320
DOC_MAPK_gen_1	285	293	PF00069	0.516
DOC_MAPK_gen_1	852	860	PF00069	0.379
DOC_MAPK_gen_1	911	919	PF00069	0.355
DOC_MAPK_JIP1_4	989	995	PF00069	0.502
DOC_MAPK_MEF2A_6	286	295	PF00069	0.492
DOC_MAPK_MEF2A_6	446	454	PF00069	0.466
DOC_PP1_RVXF_1	891	897	PF00149	0.381
DOC_PP4_FxxP_1	303	306	PF00568	0.508
DOC_PP4_FxxP_1	490	493	PF00568	0.412
DOC_PP4_FxxP_1	753	756	PF00568	0.557
DOC_USP7_MATH_1	199	203	PF00917	0.488
DOC_USP7_MATH_1	569	573	PF00917	0.600
DOC_USP7_MATH_1	782	786	PF00917	0.310
DOC_USP7_MATH_1	840	844	PF00917	0.427
DOC_USP7_MATH_1	885	889	PF00917	0.405
DOC_USP7_UBL2_3	13	17	PF12436	0.605
DOC_USP7_UBL2_3	27	31	PF12436	0.519
DOC_USP7_UBL2_3	529	533	PF12436	0.535
DOC_USP7_UBL2_3	846	850	PF12436	0.340
DOC_USP7_UBL2_3	908	912	PF12436	0.364
DOC_USP7_UBL2_3	97	101	PF12436	0.330
DOC_WW_Pin1_4	399	404	PF00397	0.575
DOC_WW_Pin1_4	489	494	PF00397	0.491
DOC_WW_Pin1_4	507	512	PF00397	0.413
DOC_WW_Pin1_4	521	526	PF00397	0.405
DOC_WW_Pin1_4	728	733	PF00397	0.535
DOC_WW_Pin1_4	798	803	PF00397	0.427
DOC_WW_Pin1_4	880	885	PF00397	0.345
LIG_14-3-3_CanoR_1	24	30	PF00244	0.602
LIG_14-3-3_CanoR_1	438	444	PF00244	0.433
LIG_14-3-3_CanoR_1	540	548	PF00244	0.543
LIG_14-3-3_CanoR_1	913	920	PF00244	0.409
LIG_Actin_WH2_2	26	41	PF00022	0.613
LIG_Actin_WH2_2	525	542	PF00022	0.346
LIG_Actin_WH2_2	837	854	PF00022	0.416
LIG_APCC_ABBA_1	391	396	PF00400	0.490
LIG_BRCT_BRCA1_1	763	767	PF00533	0.605
LIG_BRCT_BRCA1_1	787	791	PF00533	0.349
LIG_BRCT_BRCA1_1	914	918	PF00533	0.320
LIG_EH_1	557	561	PF12763	0.414
LIG_EH1_1	450	458	PF00400	0.328
LIG_eIF4E_1	975	981	PF01652	0.379
LIG_FHA_1	167	173	PF00498	0.391
LIG_FHA_1	217	223	PF00498	0.288
LIG_FHA_1	24	30	PF00498	0.526
LIG_FHA_1	286	292	PF00498	0.460
LIG_FHA_1	294	300	PF00498	0.353
LIG_FHA_1	3	9	PF00498	0.648
LIG_FHA_1	322	328	PF00498	0.443
LIG_FHA_1	42	48	PF00498	0.587
LIG_FHA_1	429	435	PF00498	0.405
LIG_FHA_1	569	575	PF00498	0.463
LIG_FHA_1	577	583	PF00498	0.401
LIG_FHA_1	613	619	PF00498	0.702
LIG_FHA_1	625	631	PF00498	0.554
LIG_FHA_1	881	887	PF00498	0.373
LIG_FHA_2	121	127	PF00498	0.344
LIG_FHA_2	180	186	PF00498	0.303
LIG_FHA_2	438	444	PF00498	0.327
LIG_FHA_2	593	599	PF00498	0.737
LIG_FHA_2	603	609	PF00498	0.603
LIG_FHA_2	682	688	PF00498	0.354
LIG_FHA_2	729	735	PF00498	0.598
LIG_GBD_Chelix_1	426	434	PF00786	0.402
LIG_LIR_Apic_2	300	306	PF02991	0.522
LIG_LIR_Apic_2	751	756	PF02991	0.581
LIG_LIR_Gen_1	367	376	PF02991	0.453
LIG_LIR_Gen_1	382	391	PF02991	0.263
LIG_LIR_Gen_1	687	697	PF02991	0.415
LIG_LIR_Gen_1	905	914	PF02991	0.322
LIG_LIR_Nem_3	276	280	PF02991	0.405
LIG_LIR_Nem_3	367	371	PF02991	0.475
LIG_LIR_Nem_3	382	386	PF02991	0.270
LIG_LIR_Nem_3	402	407	PF02991	0.539
LIG_LIR_Nem_3	458	464	PF02991	0.461
LIG_LIR_Nem_3	526	531	PF02991	0.428
LIG_LIR_Nem_3	577	583	PF02991	0.473
LIG_LIR_Nem_3	687	692	PF02991	0.394
LIG_LIR_Nem_3	752	758	PF02991	0.597
LIG_LIR_Nem_3	905	909	PF02991	0.345
LIG_NRBOX	994	1000	PF00104	0.407
LIG_PCNA_yPIPBox_3	930	939	PF02747	0.349
LIG_PDZ_Class_2	1011	1016	PF00595	0.544
LIG_Pex14_2	580	584	PF04695	0.495
LIG_REV1ctd_RIR_1	100	108	PF16727	0.345
LIG_REV1ctd_RIR_1	54	64	PF16727	0.656
LIG_SH2_CRK	387	391	PF00017	0.464
LIG_SH2_CRK	404	408	PF00017	0.300
LIG_SH2_CRK	906	910	PF00017	0.345
LIG_SH2_NCK_1	683	687	PF00017	0.351
LIG_SH2_SRC	107	110	PF00017	0.368
LIG_SH2_SRC	385	388	PF00017	0.437
LIG_SH2_STAP1	387	391	PF00017	0.456
LIG_SH2_STAP1	793	797	PF00017	0.427
LIG_SH2_STAP1	976	980	PF00017	0.419
LIG_SH2_STAT3	538	541	PF00017	0.547
LIG_SH2_STAT3	863	866	PF00017	0.379
LIG_SH2_STAT5	122	125	PF00017	0.427
LIG_SH2_STAT5	268	271	PF00017	0.529
LIG_SH2_STAT5	368	371	PF00017	0.503
LIG_SH2_STAT5	385	388	PF00017	0.322
LIG_SH2_STAT5	538	541	PF00017	0.421
LIG_SH2_STAT5	681	684	PF00017	0.337
LIG_SH2_STAT5	730	733	PF00017	0.474
LIG_SH2_STAT5	863	866	PF00017	0.320
LIG_SH2_STAT5	947	950	PF00017	0.345
LIG_SH3_3	505	511	PF00018	0.474
LIG_SH3_3	519	525	PF00018	0.364
LIG_SH3_3	615	621	PF00018	0.752
LIG_SH3_3	631	637	PF00018	0.517
LIG_SH3_3	658	664	PF00018	0.479
LIG_SH3_3	799	805	PF00018	0.345
LIG_SH3_3	876	882	PF00018	0.379
LIG_SH3_3	939	945	PF00018	0.379
LIG_SUMO_SIM_anti_2	202	208	PF11976	0.442
LIG_SUMO_SIM_anti_2	288	297	PF11976	0.512
LIG_SUMO_SIM_anti_2	428	436	PF11976	0.388
LIG_SUMO_SIM_anti_2	785	791	PF11976	0.320
LIG_SUMO_SIM_anti_2	977	982	PF11976	0.419
LIG_SUMO_SIM_par_1	430	436	PF11976	0.440
LIG_TRAF2_1	84	87	PF00917	0.626
LIG_UBA3_1	222	227	PF00899	0.350
LIG_UBA3_1	236	244	PF00899	0.276
LIG_WRC_WIRS_1	528	533	PF05994	0.521
LIG_WRC_WIRS_1	686	691	PF05994	0.479
MOD_CDK_SPK_2	489	494	PF00069	0.491
MOD_CDK_SPK_2	728	733	PF00069	0.530
MOD_CK1_1	164	170	PF00069	0.414
MOD_CK1_1	455	461	PF00069	0.454
MOD_CK1_1	510	516	PF00069	0.545
MOD_CK1_1	639	645	PF00069	0.394
MOD_CK1_1	688	694	PF00069	0.506
MOD_CK1_1	785	791	PF00069	0.320
MOD_CK1_1	907	913	PF00069	0.403
MOD_CK2_1	120	126	PF00069	0.351
MOD_CK2_1	164	170	PF00069	0.345
MOD_CK2_1	340	346	PF00069	0.386
MOD_CK2_1	539	545	PF00069	0.540
MOD_CK2_1	608	614	PF00069	0.647
MOD_CK2_1	80	86	PF00069	0.636
MOD_CK2_1	867	873	PF00069	0.323
MOD_Cter_Amidation	230	233	PF01082	0.416
MOD_Cter_Amidation	418	421	PF01082	0.437
MOD_GlcNHglycan	166	169	PF01048	0.392
MOD_GlcNHglycan	241	244	PF01048	0.410
MOD_GlcNHglycan	435	438	PF01048	0.441
MOD_GlcNHglycan	598	601	PF01048	0.751
MOD_GlcNHglycan	618	621	PF01048	0.620
MOD_GlcNHglycan	643	646	PF01048	0.400
MOD_GlcNHglycan	703	707	PF01048	0.435
MOD_GlcNHglycan	740	743	PF01048	0.520
MOD_GlcNHglycan	768	771	PF01048	0.588
MOD_GSK3_1	175	182	PF00069	0.393
MOD_GSK3_1	293	300	PF00069	0.395
MOD_GSK3_1	319	326	PF00069	0.415
MOD_GSK3_1	345	352	PF00069	0.482
MOD_GSK3_1	395	402	PF00069	0.360
MOD_GSK3_1	428	435	PF00069	0.454
MOD_GSK3_1	523	530	PF00069	0.422
MOD_GSK3_1	540	547	PF00069	0.470
MOD_GSK3_1	570	577	PF00069	0.502
MOD_GSK3_1	592	599	PF00069	0.607
MOD_GSK3_1	608	615	PF00069	0.661
MOD_GSK3_1	626	633	PF00069	0.588
MOD_GSK3_1	636	643	PF00069	0.493
MOD_GSK3_1	681	688	PF00069	0.412
MOD_GSK3_1	76	83	PF00069	0.613
MOD_GSK3_1	761	768	PF00069	0.517
MOD_GSK3_1	804	811	PF00069	0.316
MOD_GSK3_1	840	847	PF00069	0.291
MOD_N-GLC_1	175	180	PF02516	0.422
MOD_N-GLC_1	601	606	PF02516	0.527
MOD_N-GLC_1	748	753	PF02516	0.629
MOD_N-GLC_1	777	782	PF02516	0.416
MOD_NEK2_1	179	184	PF00069	0.329
MOD_NEK2_1	239	244	PF00069	0.379
MOD_NEK2_1	260	265	PF00069	0.536
MOD_NEK2_1	327	332	PF00069	0.405
MOD_NEK2_1	340	345	PF00069	0.325
MOD_NEK2_1	386	391	PF00069	0.345
MOD_NEK2_1	574	579	PF00069	0.469
MOD_NEK2_1	641	646	PF00069	0.385
MOD_NEK2_1	859	864	PF00069	0.424
MOD_NEK2_1	867	872	PF00069	0.440
MOD_NEK2_1	937	942	PF00069	0.416
MOD_NEK2_2	885	890	PF00069	0.427
MOD_OFUCOSY	320	325	PF10250	0.483
MOD_PIKK_1	199	205	PF00454	0.448
MOD_PIKK_1	231	237	PF00454	0.379
MOD_PIKK_1	312	318	PF00454	0.494
MOD_PIKK_1	406	412	PF00454	0.443
MOD_PIKK_1	473	479	PF00454	0.396
MOD_PIKK_1	574	580	PF00454	0.540
MOD_PK_1	25	31	PF00069	0.659
MOD_PK_1	39	45	PF00069	0.557
MOD_PK_1	438	444	PF00069	0.510
MOD_PKA_1	285	291	PF00069	0.498
MOD_PKA_1	912	918	PF00069	0.320
MOD_PKA_2	193	199	PF00069	0.383
MOD_PKA_2	23	29	PF00069	0.599
MOD_PKA_2	437	443	PF00069	0.433
MOD_PKA_2	539	545	PF00069	0.489
MOD_PKA_2	761	767	PF00069	0.569
MOD_PKA_2	912	918	PF00069	0.409
MOD_Plk_1	103	109	PF00069	0.309
MOD_Plk_1	175	181	PF00069	0.422
MOD_Plk_1	216	222	PF00069	0.314
MOD_Plk_1	260	266	PF00069	0.501
MOD_Plk_1	345	351	PF00069	0.404
MOD_Plk_1	569	575	PF00069	0.509
MOD_Plk_1	601	607	PF00069	0.769
MOD_Plk_1	748	754	PF00069	0.516
MOD_Plk_1	777	783	PF00069	0.482
MOD_Plk_1	793	799	PF00069	0.297
MOD_Plk_2-3	608	614	PF00069	0.480
MOD_Plk_4	161	167	PF00069	0.384
MOD_Plk_4	175	181	PF00069	0.421
MOD_Plk_4	25	31	PF00069	0.630
MOD_Plk_4	323	329	PF00069	0.399
MOD_Plk_4	345	351	PF00069	0.499
MOD_Plk_4	386	392	PF00069	0.472
MOD_Plk_4	428	434	PF00069	0.488
MOD_Plk_4	452	458	PF00069	0.328
MOD_Plk_4	527	533	PF00069	0.515
MOD_Plk_4	534	540	PF00069	0.479
MOD_Plk_4	570	576	PF00069	0.586
MOD_Plk_4	636	642	PF00069	0.418
MOD_Plk_4	681	687	PF00069	0.363
MOD_Plk_4	782	788	PF00069	0.330
MOD_Plk_4	808	814	PF00069	0.364
MOD_Plk_4	859	865	PF00069	0.338
MOD_Plk_4	885	891	PF00069	0.381
MOD_Plk_4	932	938	PF00069	0.427
MOD_ProDKin_1	399	405	PF00069	0.568
MOD_ProDKin_1	489	495	PF00069	0.488
MOD_ProDKin_1	507	513	PF00069	0.416
MOD_ProDKin_1	521	527	PF00069	0.405
MOD_ProDKin_1	728	734	PF00069	0.546
MOD_ProDKin_1	798	804	PF00069	0.427
MOD_ProDKin_1	880	886	PF00069	0.345
MOD_SUMO_for_1	252	255	PF00179	0.555
MOD_SUMO_for_1	350	353	PF00179	0.424
MOD_SUMO_rev_2	134	140	PF00179	0.387
MOD_SUMO_rev_2	183	189	PF00179	0.427
MOD_SUMO_rev_2	530	537	PF00179	0.444
MOD_SUMO_rev_2	96	103	PF00179	0.310
TRG_DiLeu_BaEn_2	246	252	PF01217	0.427
TRG_DiLeu_BaEn_2	496	502	PF01217	0.495
TRG_DiLeu_BaEn_4	675	681	PF01217	0.397
TRG_DiLeu_BaLyEn_6	799	804	PF01217	0.320
TRG_DiLeu_BaLyEn_6	890	895	PF01217	0.416
TRG_ENDOCYTIC_2	368	371	PF00928	0.503
TRG_ENDOCYTIC_2	387	390	PF00928	0.253
TRG_ENDOCYTIC_2	404	407	PF00928	0.308
TRG_ENDOCYTIC_2	683	686	PF00928	0.449
TRG_ENDOCYTIC_2	906	909	PF00928	0.345
TRG_ENDOCYTIC_2	975	978	PF00928	0.427
TRG_ER_diArg_1	156	158	PF00400	0.355
TRG_ER_diArg_1	53	55	PF00400	0.563
TRG_ER_diArg_1	736	738	PF00400	0.413
TRG_ER_diArg_1	815	818	PF00400	0.412
TRG_ER_diArg_1	877	879	PF00400	0.368
TRG_NES_CRM1_1	170	185	PF08389	0.416
TRG_NES_CRM1_1	916	930	PF08389	0.362
TRG_NLS_MonoExtC_3	910	916	PF00514	0.345
TRG_NLS_MonoExtN_4	908	915	PF00514	0.345
TRG_Pf-PMV_PEXEL_1	1001	1006	PF00026	0.487
TRG_Pf-PMV_PEXEL_1	141	145	PF00026	0.459
TRG_Pf-PMV_PEXEL_1	173	177	PF00026	0.364
TRG_Pf-PMV_PEXEL_1	342	346	PF00026	0.371
TRG_Pf-PMV_PEXEL_1	710	714	PF00026	0.489
TRG_Pf-PMV_PEXEL_1	817	821	PF00026	0.349
TRG_Pf-PMV_PEXEL_1	872	876	PF00026	0.448

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IIU4	Leptomonas seymouri	76%	100%
A0A0S4KHU7	Bodo saltans	32%	100%
A0A1X0NVY5	Trypanosomatidae	46%	100%
A0A422NWJ9	Trypanosoma rangeli	44%	100%
A4HDS8	Leishmania braziliensis	84%	100%
A4I126	Leishmania infantum	99%	100%
E9AX63	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
Q4QAB3	Leishmania major	95%	100%
V5D5L5	Trypanosoma cruzi	46%	100%