tRNA:m(4)X modification enzyme TRM13

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

methyltransferase domain containing protein, putative

Species:

Leishmania donovani

UniProt:

A0A3S7WY71_LEIDO

TriTrypDb:

LdBPK_240560.1 , LdCL_240010400 , LDHU3_24.0670

Length:

512

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

A0A3S7WY71

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7WY71

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006396	RNA processing	6	2
GO:0006399	tRNA metabolic process	7	2
GO:0006400	tRNA modification	6	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008033	tRNA processing	8	2
GO:0008152	metabolic process	1	2
GO:0009451	RNA modification	5	2
GO:0009987	cellular process	1	2
GO:0016070	RNA metabolic process	5	2
GO:0030488	tRNA methylation	5	2
GO:0032259	methylation	2	2
GO:0034470	ncRNA processing	7	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034660	ncRNA metabolic process	6	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0043414	macromolecule methylation	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0046483	heterocycle metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	13
GO:0005488	binding	1	13
GO:0008168	methyltransferase activity	4	13
GO:0008171	O-methyltransferase activity	5	13
GO:0008173	RNA methyltransferase activity	4	13
GO:0008175	tRNA methyltransferase activity	5	13
GO:0016740	transferase activity	2	13
GO:0016741	transferase activity, transferring one-carbon groups	3	13
GO:0043167	ion binding	2	13
GO:0043169	cation binding	3	13
GO:0046872	metal ion binding	4	13
GO:0062105	RNA 2'-O-methyltransferase activity	5	13
GO:0106050	tRNA 2'-O-methyltransferase activity	6	13
GO:0140098	catalytic activity, acting on RNA	3	13
GO:0140101	catalytic activity, acting on a tRNA	4	13
GO:0140640	catalytic activity, acting on a nucleic acid	2	13

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	98	102	PF00656	0.454
CLV_NRD_NRD_1	192	194	PF00675	0.333
CLV_NRD_NRD_1	262	264	PF00675	0.511
CLV_NRD_NRD_1	28	30	PF00675	0.435
CLV_NRD_NRD_1	331	333	PF00675	0.275
CLV_PCSK_FUR_1	260	264	PF00082	0.490
CLV_PCSK_KEX2_1	192	194	PF00082	0.333
CLV_PCSK_KEX2_1	262	264	PF00082	0.511
CLV_PCSK_KEX2_1	331	333	PF00082	0.276
CLV_PCSK_PC7_1	327	333	PF00082	0.305
CLV_PCSK_SKI1_1	192	196	PF00082	0.535
CLV_PCSK_SKI1_1	327	331	PF00082	0.319
CLV_PCSK_SKI1_1	358	362	PF00082	0.338
CLV_Separin_Metazoa	252	256	PF03568	0.435
CLV_Separin_Metazoa	373	377	PF03568	0.519
DEG_Nend_UBRbox_3	1	3	PF02207	0.526
DEG_SCF_FBW7_1	352	359	PF00400	0.440
DOC_CKS1_1	353	358	PF01111	0.440
DOC_PP1_RVXF_1	342	349	PF00149	0.519
DOC_PP1_RVXF_1	477	483	PF00149	0.427
DOC_PP2B_LxvP_1	178	181	PF13499	0.438
DOC_PP2B_PxIxI_1	384	390	PF00149	0.533
DOC_PP4_FxxP_1	140	143	PF00568	0.346
DOC_USP7_MATH_1	152	156	PF00917	0.329
DOC_USP7_MATH_1	210	214	PF00917	0.424
DOC_USP7_MATH_1	233	237	PF00917	0.436
DOC_USP7_MATH_1	308	312	PF00917	0.412
DOC_USP7_MATH_1	380	384	PF00917	0.453
DOC_USP7_MATH_1	448	452	PF00917	0.476
DOC_USP7_MATH_1	473	477	PF00917	0.440
DOC_USP7_MATH_1	77	81	PF00917	0.408
DOC_WW_Pin1_4	141	146	PF00397	0.439
DOC_WW_Pin1_4	352	357	PF00397	0.499
DOC_WW_Pin1_4	498	503	PF00397	0.461
LIG_14-3-3_CanoR_1	113	122	PF00244	0.505
LIG_14-3-3_CanoR_1	157	163	PF00244	0.439
LIG_14-3-3_CanoR_1	206	210	PF00244	0.491
LIG_14-3-3_CanoR_1	331	337	PF00244	0.498
LIG_14-3-3_CanoR_1	347	353	PF00244	0.525
LIG_14-3-3_CanoR_1	426	434	PF00244	0.548
LIG_14-3-3_CanoR_1	458	464	PF00244	0.440
LIG_14-3-3_CanoR_1	9	15	PF00244	0.484
LIG_Actin_WH2_2	241	257	PF00022	0.472
LIG_AP2alpha_2	304	306	PF02296	0.365
LIG_APCC_ABBA_1	340	345	PF00400	0.506
LIG_BIR_III_2	350	354	PF00653	0.519
LIG_BRCT_BRCA1_1	16	20	PF00533	0.347
LIG_FHA_1	142	148	PF00498	0.464
LIG_FHA_1	151	157	PF00498	0.499
LIG_FHA_1	159	165	PF00498	0.542
LIG_FHA_1	227	233	PF00498	0.434
LIG_FHA_1	337	343	PF00498	0.506
LIG_FHA_1	357	363	PF00498	0.388
LIG_FHA_2	400	406	PF00498	0.440
LIG_FHA_2	60	66	PF00498	0.497
LIG_FHA_2	98	104	PF00498	0.452
LIG_KLC1_Yacidic_2	401	405	PF13176	0.440
LIG_LIR_Apic_2	137	143	PF02991	0.367
LIG_LIR_Apic_2	401	406	PF02991	0.437
LIG_LIR_Gen_1	220	228	PF02991	0.376
LIG_LIR_Nem_3	220	224	PF02991	0.397
LIG_LIR_Nem_3	226	231	PF02991	0.410
LIG_MYND_1	316	320	PF01753	0.440
LIG_PCNA_TLS_4	397	404	PF02747	0.440
LIG_Pex14_1	16	20	PF04695	0.329
LIG_RPA_C_Fungi	24	36	PF08784	0.356
LIG_SH2_CRK	11	15	PF00017	0.414
LIG_SH2_PTP2	403	406	PF00017	0.248
LIG_SH2_SRC	403	406	PF00017	0.354
LIG_SH2_STAP1	160	164	PF00017	0.394
LIG_SH2_STAP1	228	232	PF00017	0.338
LIG_SH2_STAT5	160	163	PF00017	0.362
LIG_SH2_STAT5	183	186	PF00017	0.468
LIG_SH2_STAT5	228	231	PF00017	0.400
LIG_SH2_STAT5	403	406	PF00017	0.295
LIG_SH2_STAT5	459	462	PF00017	0.229
LIG_SH2_STAT5	488	491	PF00017	0.306
LIG_SH2_STAT5	495	498	PF00017	0.285
LIG_SH3_2	353	358	PF14604	0.377
LIG_SH3_3	126	132	PF00018	0.529
LIG_SH3_3	146	152	PF00018	0.512
LIG_SH3_3	299	305	PF00018	0.336
LIG_SH3_3	350	356	PF00018	0.377
LIG_SH3_3	386	392	PF00018	0.303
LIG_SH3_3	458	464	PF00018	0.267
LIG_SUMO_SIM_anti_2	144	150	PF11976	0.277
LIG_SUMO_SIM_par_1	223	230	PF11976	0.396
LIG_WRC_WIRS_1	228	233	PF05994	0.416
LIG_WRC_WIRS_1	78	83	PF05994	0.428
MOD_CDK_SPK_2	498	503	PF00069	0.295
MOD_CDK_SPxK_1	352	358	PF00069	0.267
MOD_CDK_SPxxK_3	498	505	PF00069	0.329
MOD_CK1_1	396	402	PF00069	0.258
MOD_CK1_1	453	459	PF00069	0.283
MOD_CK1_1	54	60	PF00069	0.555
MOD_CK1_1	80	86	PF00069	0.438
MOD_CK2_1	459	465	PF00069	0.366
MOD_GlcNHglycan	134	137	PF01048	0.403
MOD_GlcNHglycan	198	201	PF01048	0.505
MOD_GlcNHglycan	368	371	PF01048	0.479
MOD_GlcNHglycan	409	412	PF01048	0.404
MOD_GlcNHglycan	416	419	PF01048	0.394
MOD_GlcNHglycan	443	446	PF01048	0.297
MOD_GlcNHglycan	448	451	PF01048	0.302
MOD_GlcNHglycan	87	90	PF01048	0.654
MOD_GSK3_1	10	17	PF00069	0.526
MOD_GSK3_1	332	339	PF00069	0.341
MOD_GSK3_1	352	359	PF00069	0.175
MOD_GSK3_1	446	453	PF00069	0.330
MOD_GSK3_1	47	54	PF00069	0.530
MOD_GSK3_1	59	66	PF00069	0.480
MOD_GSK3_1	87	94	PF00069	0.534
MOD_N-GLC_1	430	435	PF02516	0.328
MOD_N-GLC_2	49	51	PF02516	0.533
MOD_N-GLC_2	504	506	PF02516	0.359
MOD_NEK2_1	227	232	PF00069	0.501
MOD_NEK2_1	336	341	PF00069	0.377
MOD_NEK2_1	366	371	PF00069	0.303
MOD_NEK2_1	87	92	PF00069	0.438
MOD_NEK2_1	95	100	PF00069	0.473
MOD_NEK2_2	152	157	PF00069	0.424
MOD_NEK2_2	430	435	PF00069	0.389
MOD_PIKK_1	114	120	PF00454	0.496
MOD_PIKK_1	473	479	PF00454	0.267
MOD_PIKK_1	80	86	PF00454	0.404
MOD_PIKK_1	87	93	PF00454	0.411
MOD_PKA_2	205	211	PF00069	0.571
MOD_PKA_2	254	260	PF00069	0.466
MOD_PKA_2	346	352	PF00069	0.382
MOD_PKA_2	425	431	PF00069	0.336
MOD_PKB_1	325	333	PF00069	0.396
MOD_Plk_1	204	210	PF00069	0.461
MOD_Plk_1	233	239	PF00069	0.438
MOD_Plk_1	430	436	PF00069	0.407
MOD_Plk_4	10	16	PF00069	0.499
MOD_Plk_4	227	233	PF00069	0.352
MOD_Plk_4	297	303	PF00069	0.338
MOD_Plk_4	399	405	PF00069	0.267
MOD_Plk_4	63	69	PF00069	0.564
MOD_ProDKin_1	141	147	PF00069	0.434
MOD_ProDKin_1	352	358	PF00069	0.348
MOD_ProDKin_1	498	504	PF00069	0.295
TRG_DiLeu_BaEn_1	220	225	PF01217	0.385
TRG_DiLeu_BaEn_1	63	68	PF01217	0.429
TRG_DiLeu_BaLyEn_6	142	147	PF01217	0.332
TRG_DiLeu_LyEn_5	220	225	PF01217	0.383
TRG_ENDOCYTIC_2	11	14	PF00928	0.376
TRG_ENDOCYTIC_2	228	231	PF00928	0.338
TRG_ER_diArg_1	192	194	PF00400	0.333
TRG_ER_diArg_1	260	263	PF00400	0.536
TRG_ER_diArg_1	325	328	PF00400	0.305
TRG_ER_diArg_1	331	334	PF00400	0.303
TRG_Pf-PMV_PEXEL_1	105	110	PF00026	0.456
TRG_Pf-PMV_PEXEL_1	376	381	PF00026	0.396

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A1X0NJE0	Trypanosomatidae	32%	100%
A0A3Q8IG18	Leishmania donovani	53%	94%
A4I0P4	Leishmania infantum	53%	94%
A4I0P5	Leishmania infantum	100%	100%
D0A723	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	100%
E9AIR1	Leishmania braziliensis	78%	100%
E9AWP4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	52%	94%
E9AWP5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
Q4QAS6	Leishmania major	93%	100%
Q4QAS7	Leishmania major	52%	94%
V5BR90	Trypanosoma cruzi	35%	97%