Mitochondrial DNA primase, putative

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

mitochondrial DNA primase, putative

Species:

Leishmania donovani

UniProt:

A0A3S7WXR7_LEIDO

TriTrypDb:

LdBPK_230850.1 , LdCL_230015100 , LDHU3_23.1150

Length:

533

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	1
GO:0005730	nucleolus	5	1
GO:0005759	mitochondrial matrix	5	1
GO:0020023	kinetoplast	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A0A3S7WXR7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7WXR7

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006259	DNA metabolic process	4	1
GO:0006260	DNA replication	5	1
GO:0006261	DNA-templated DNA replication	6	1
GO:0006264	mitochondrial DNA replication	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009987	cellular process	1	1
GO:0032042	mitochondrial DNA metabolic process	5	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	5
GO:0016740	transferase activity	2	5
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	5
GO:0016779	nucleotidyltransferase activity	4	5
GO:0016787	hydrolase activity	2	11
GO:0016817	hydrolase activity, acting on acid anhydrides	3	11
GO:0034062	5'-3' RNA polymerase activity	5	5
GO:0097747	RNA polymerase activity	4	5
GO:0140098	catalytic activity, acting on RNA	3	5
GO:0140640	catalytic activity, acting on a nucleic acid	2	5
GO:0003896	DNA primase activity	7	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	120	124	PF00656	0.571
CLV_C14_Caspase3-7	421	425	PF00656	0.354
CLV_NRD_NRD_1	101	103	PF00675	0.708
CLV_NRD_NRD_1	128	130	PF00675	0.442
CLV_NRD_NRD_1	197	199	PF00675	0.478
CLV_NRD_NRD_1	430	432	PF00675	0.436
CLV_NRD_NRD_1	5	7	PF00675	0.717
CLV_NRD_NRD_1	520	522	PF00675	0.728
CLV_NRD_NRD_1	531	533	PF00675	0.736
CLV_PCSK_KEX2_1	128	130	PF00082	0.452
CLV_PCSK_KEX2_1	465	467	PF00082	0.617
CLV_PCSK_KEX2_1	531	533	PF00082	0.745
CLV_PCSK_PC1ET2_1	465	467	PF00082	0.649
CLV_PCSK_SKI1_1	198	202	PF00082	0.392
CLV_PCSK_SKI1_1	328	332	PF00082	0.511
CLV_PCSK_SKI1_1	381	385	PF00082	0.479
CLV_PCSK_SKI1_1	521	525	PF00082	0.727
CLV_PCSK_SKI1_1	527	531	PF00082	0.729
DEG_APCC_DBOX_1	197	205	PF00400	0.498
DEG_APCC_KENBOX_2	283	287	PF00400	0.451
DEG_Nend_Nbox_1	1	3	PF02207	0.697
DEG_SCF_FBW7_2	204	210	PF00400	0.498
DOC_ANK_TNKS_1	5	12	PF00023	0.775
DOC_CKS1_1	141	146	PF01111	0.439
DOC_CKS1_1	204	209	PF01111	0.494
DOC_CYCLIN_RxL_1	325	333	PF00134	0.452
DOC_CYCLIN_yCln2_LP_2	414	420	PF00134	0.438
DOC_MAPK_DCC_7	258	268	PF00069	0.430
DOC_MAPK_gen_1	284	294	PF00069	0.576
DOC_MAPK_gen_1	378	388	PF00069	0.445
DOC_MAPK_gen_1	431	437	PF00069	0.447
DOC_MAPK_gen_1	465	472	PF00069	0.660
DOC_MAPK_HePTP_8	165	177	PF00069	0.401
DOC_MAPK_MEF2A_6	168	177	PF00069	0.403
DOC_MAPK_MEF2A_6	262	270	PF00069	0.432
DOC_MAPK_MEF2A_6	392	399	PF00069	0.335
DOC_MAPK_MEF2A_6	465	474	PF00069	0.666
DOC_MAPK_NFAT4_5	392	400	PF00069	0.354
DOC_PP1_RVXF_1	326	333	PF00149	0.454
DOC_PP2B_LxvP_1	358	361	PF13499	0.698
DOC_PP2B_LxvP_1	470	473	PF13499	0.674
DOC_PP4_FxxP_1	138	141	PF00568	0.396
DOC_PP4_FxxP_1	294	297	PF00568	0.508
DOC_PP4_FxxP_1	332	335	PF00568	0.464
DOC_USP7_MATH_1	117	121	PF00917	0.578
DOC_USP7_MATH_1	146	150	PF00917	0.481
DOC_USP7_MATH_1	151	155	PF00917	0.512
DOC_USP7_MATH_1	314	318	PF00917	0.380
DOC_USP7_MATH_1	512	516	PF00917	0.696
DOC_USP7_MATH_1	66	70	PF00917	0.782
DOC_USP7_MATH_1	83	87	PF00917	0.616
DOC_USP7_MATH_1	9	13	PF00917	0.777
DOC_USP7_UBL2_3	432	436	PF12436	0.335
DOC_USP7_UBL2_3	524	528	PF12436	0.719
DOC_WW_Pin1_4	140	145	PF00397	0.434
DOC_WW_Pin1_4	168	173	PF00397	0.413
DOC_WW_Pin1_4	203	208	PF00397	0.491
DOC_WW_Pin1_4	224	229	PF00397	0.399
DOC_WW_Pin1_4	27	32	PF00397	0.767
LIG_14-3-3_CanoR_1	102	110	PF00244	0.628
LIG_14-3-3_CanoR_1	287	294	PF00244	0.566
LIG_14-3-3_CanoR_1	328	333	PF00244	0.529
LIG_14-3-3_CanoR_1	381	388	PF00244	0.468
LIG_Actin_WH2_2	216	233	PF00022	0.429
LIG_APCC_ABBA_1	291	296	PF00400	0.403
LIG_deltaCOP1_diTrp_1	295	303	PF00928	0.434
LIG_deltaCOP1_diTrp_1	390	394	PF00928	0.355
LIG_deltaCOP1_diTrp_1	443	449	PF00928	0.335
LIG_FHA_1	102	108	PF00498	0.509
LIG_FHA_1	219	225	PF00498	0.494
LIG_FHA_1	286	292	PF00498	0.460
LIG_FHA_2	169	175	PF00498	0.487
LIG_FHA_2	366	372	PF00498	0.507
LIG_FHA_2	382	388	PF00498	0.287
LIG_LIR_Apic_2	331	335	PF02991	0.565
LIG_LIR_Gen_1	243	252	PF02991	0.368
LIG_LIR_Gen_1	324	334	PF02991	0.458
LIG_LIR_Gen_1	443	454	PF02991	0.335
LIG_LIR_Gen_1	87	94	PF02991	0.598
LIG_LIR_Nem_3	243	249	PF02991	0.360
LIG_LIR_Nem_3	324	329	PF02991	0.453
LIG_LIR_Nem_3	393	397	PF02991	0.354
LIG_LIR_Nem_3	443	449	PF02991	0.335
LIG_LIR_Nem_3	458	464	PF02991	0.335
LIG_LIR_Nem_3	87	93	PF02991	0.671
LIG_MAD2	199	207	PF02301	0.533
LIG_OCRL_FandH_1	248	260	PF00620	0.472
LIG_Pex14_1	299	303	PF04695	0.419
LIG_Pex14_1	460	464	PF04695	0.335
LIG_Pex14_2	255	259	PF04695	0.395
LIG_SH2_CRK	464	468	PF00017	0.513
LIG_SH2_CRK	90	94	PF00017	0.597
LIG_SH2_NCK_1	90	94	PF00017	0.597
LIG_SH2_STAP1	457	461	PF00017	0.273
LIG_SH2_STAP1	90	94	PF00017	0.709
LIG_SH3_3	138	144	PF00018	0.431
LIG_SH3_3	150	156	PF00018	0.455
LIG_SH3_3	201	207	PF00018	0.532
LIG_SH3_3	317	323	PF00018	0.483
LIG_SH3_3	360	366	PF00018	0.623
LIG_SH3_3	498	504	PF00018	0.673
LIG_SUMO_SIM_anti_2	171	178	PF11976	0.413
LIG_TRAF2_1	12	15	PF00917	0.826
LIG_UBA3_1	1	7	PF00899	0.781
LIG_UBA3_1	276	284	PF00899	0.393
LIG_WRC_WIRS_1	329	334	PF05994	0.587
MOD_CDK_SPxK_1	203	209	PF00069	0.497
MOD_CDK_SPxK_1	27	33	PF00069	0.613
MOD_CDK_SPxxK_3	224	231	PF00069	0.411
MOD_CK1_1	25	31	PF00069	0.716
MOD_CK1_1	324	330	PF00069	0.582
MOD_CK1_1	46	52	PF00069	0.701
MOD_CK1_1	515	521	PF00069	0.762
MOD_CK1_1	98	104	PF00069	0.649
MOD_CK2_1	365	371	PF00069	0.524
MOD_CK2_1	381	387	PF00069	0.335
MOD_CK2_1	71	77	PF00069	0.748
MOD_CK2_1	9	15	PF00069	0.741
MOD_CMANNOS	391	394	PF00535	0.335
MOD_DYRK1A_RPxSP_1	168	172	PF00069	0.544
MOD_GlcNHglycan	131	134	PF01048	0.590
MOD_GlcNHglycan	149	152	PF01048	0.348
MOD_GlcNHglycan	288	291	PF01048	0.445
MOD_GlcNHglycan	438	441	PF01048	0.417
MOD_GlcNHglycan	461	464	PF01048	0.417
MOD_GlcNHglycan	517	520	PF01048	0.685
MOD_GlcNHglycan	74	77	PF01048	0.789
MOD_GSK3_1	147	154	PF00069	0.456
MOD_GSK3_1	18	25	PF00069	0.830
MOD_GSK3_1	324	331	PF00069	0.509
MOD_GSK3_1	455	462	PF00069	0.450
MOD_GSK3_1	60	67	PF00069	0.777
MOD_GSK3_1	97	104	PF00069	0.599
MOD_N-GLC_1	285	290	PF02516	0.477
MOD_N-GLC_1	374	379	PF02516	0.302
MOD_N-GLC_1	95	100	PF02516	0.732
MOD_PIKK_1	218	224	PF00454	0.404
MOD_PIKK_1	43	49	PF00454	0.705
MOD_PKA_1	231	237	PF00069	0.389
MOD_PKA_2	101	107	PF00069	0.618
MOD_PKA_2	286	292	PF00069	0.585
MOD_Plk_1	374	380	PF00069	0.319
MOD_Plk_2-3	22	28	PF00069	0.822
MOD_ProDKin_1	140	146	PF00069	0.436
MOD_ProDKin_1	168	174	PF00069	0.410
MOD_ProDKin_1	203	209	PF00069	0.497
MOD_ProDKin_1	224	230	PF00069	0.405
MOD_ProDKin_1	27	33	PF00069	0.767
MOD_SUMO_for_1	208	211	PF00179	0.479
MOD_SUMO_rev_2	10	17	PF00179	0.733
TRG_ENDOCYTIC_2	187	190	PF00928	0.545
TRG_ENDOCYTIC_2	464	467	PF00928	0.489
TRG_ENDOCYTIC_2	90	93	PF00928	0.594
TRG_ER_diArg_1	128	130	PF00400	0.460
TRG_ER_diArg_1	530	532	PF00400	0.769
TRG_NES_CRM1_1	213	226	PF08389	0.430
TRG_Pf-PMV_PEXEL_1	328	333	PF00026	0.484
TRG_Pf-PMV_PEXEL_1	378	382	PF00026	0.447

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I0R5	Leptomonas seymouri	68%	77%
A0A0S4IY41	Bodo saltans	44%	100%
A0A1X0NXL3	Trypanosomatidae	55%	85%
A4HCT9	Leishmania braziliensis	83%	100%
A4I0B9	Leishmania infantum	100%	100%
C9ZV17	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	62%	85%
E9AW83	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
Q4QB95	Leishmania major	93%	100%
V5D725	Trypanosoma cruzi	62%	86%