E3 ubiquitin-protein ligase KCMF1, putative

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

E3 ubiquitin-protein ligase KCMF1, putative

Species:

Leishmania donovani

UniProt:

A0A3S7WVR9_LEIDO

TriTrypDb:

LdBPK_200320.1 , LdCL_200008000 , LDHU3_20.0360

Length:

799

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005622	intracellular anatomical structure	2	1
GO:0005737	cytoplasm	2	1
GO:0005773	vacuole	5	1
GO:0005776	autophagosome	6	1
GO:0016234	inclusion body	3	1
GO:0016235	aggresome	4	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0043233	organelle lumen	3	1
GO:0044753	amphisome	7	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A0A3S7WVR9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7WVR9

Function

Biological processes
Term	Name	Level	Count
GO:0000422	autophagy of mitochondrion	4	1
GO:0000423	mitophagy	5	1
GO:0006914	autophagy	3	1
GO:0006996	organelle organization	4	1
GO:0007005	mitochondrion organization	5	1
GO:0007032	endosome organization	6	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0009987	cellular process	1	1
GO:0016043	cellular component organization	3	1
GO:0016050	vesicle organization	5	1
GO:0016236	macroautophagy	4	1
GO:0022411	cellular component disassembly	4	1
GO:0035973	aggrephagy	6	1
GO:0044237	cellular metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0061726	mitochondrion disassembly	6	1
GO:0061912	selective autophagy	5	1
GO:0061919	process utilizing autophagic mechanism	2	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:1903008	organelle disassembly	5	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	11
GO:0008270	zinc ion binding	6	11
GO:0043167	ion binding	2	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0046914	transition metal ion binding	5	11
GO:0005080	protein kinase C binding	6	1
GO:0005515	protein binding	2	1
GO:0019899	enzyme binding	3	1
GO:0019900	kinase binding	4	1
GO:0019901	protein kinase binding	5	1
GO:0031593	polyubiquitin modification-dependent protein binding	4	1
GO:0070530	K63-linked polyubiquitin modification-dependent protein binding	5	1
GO:0140030	modification-dependent protein binding	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	472	476	PF00656	0.593
CLV_NRD_NRD_1	180	182	PF00675	0.541
CLV_NRD_NRD_1	280	282	PF00675	0.408
CLV_NRD_NRD_1	430	432	PF00675	0.569
CLV_NRD_NRD_1	460	462	PF00675	0.745
CLV_NRD_NRD_1	500	502	PF00675	0.564
CLV_NRD_NRD_1	71	73	PF00675	0.515
CLV_PCSK_FUR_1	458	462	PF00082	0.746
CLV_PCSK_KEX2_1	280	282	PF00082	0.467
CLV_PCSK_KEX2_1	341	343	PF00082	0.403
CLV_PCSK_KEX2_1	37	39	PF00082	0.527
CLV_PCSK_KEX2_1	430	432	PF00082	0.557
CLV_PCSK_KEX2_1	458	460	PF00082	0.768
CLV_PCSK_KEX2_1	500	502	PF00082	0.483
CLV_PCSK_KEX2_1	71	73	PF00082	0.547
CLV_PCSK_KEX2_1	721	723	PF00082	0.540
CLV_PCSK_PC1ET2_1	341	343	PF00082	0.400
CLV_PCSK_PC1ET2_1	37	39	PF00082	0.527
CLV_PCSK_PC1ET2_1	721	723	PF00082	0.590
CLV_PCSK_PC7_1	717	723	PF00082	0.550
CLV_PCSK_SKI1_1	281	285	PF00082	0.473
CLV_PCSK_SKI1_1	71	75	PF00082	0.490
CLV_PCSK_SKI1_1	84	88	PF00082	0.323
DEG_APCC_DBOX_1	250	258	PF00400	0.514
DEG_SPOP_SBC_1	170	174	PF00917	0.630
DEG_SPOP_SBC_1	66	70	PF00917	0.463
DOC_CKS1_1	123	128	PF01111	0.545
DOC_CKS1_1	583	588	PF01111	0.541
DOC_CYCLIN_RxL_1	278	287	PF00134	0.516
DOC_CYCLIN_RxL_1	495	507	PF00134	0.516
DOC_CYCLIN_yClb1_LxF_4	204	210	PF00134	0.584
DOC_CYCLIN_yCln2_LP_2	120	126	PF00134	0.485
DOC_CYCLIN_yCln2_LP_2	646	652	PF00134	0.419
DOC_MAPK_gen_1	341	348	PF00069	0.382
DOC_MAPK_gen_1	436	444	PF00069	0.513
DOC_MAPK_gen_1	459	471	PF00069	0.675
DOC_MAPK_gen_1	500	506	PF00069	0.489
DOC_MAPK_MEF2A_6	341	350	PF00069	0.354
DOC_MAPK_MEF2A_6	464	471	PF00069	0.674
DOC_PP2B_LxvP_1	646	649	PF13499	0.496
DOC_PP2B_LxvP_1	770	773	PF13499	0.510
DOC_PP2B_LxvP_1	784	787	PF13499	0.594
DOC_PP4_FxxP_1	783	786	PF00568	0.551
DOC_USP7_MATH_1	170	174	PF00917	0.630
DOC_USP7_MATH_1	231	235	PF00917	0.650
DOC_USP7_MATH_1	519	523	PF00917	0.520
DOC_USP7_MATH_1	576	580	PF00917	0.576
DOC_USP7_MATH_1	678	682	PF00917	0.574
DOC_USP7_MATH_1	759	763	PF00917	0.644
DOC_USP7_UBL2_3	337	341	PF12436	0.577
DOC_WW_Pin1_4	122	127	PF00397	0.485
DOC_WW_Pin1_4	191	196	PF00397	0.700
DOC_WW_Pin1_4	370	375	PF00397	0.431
DOC_WW_Pin1_4	451	456	PF00397	0.651
DOC_WW_Pin1_4	5	10	PF00397	0.603
DOC_WW_Pin1_4	582	587	PF00397	0.511
DOC_WW_Pin1_4	744	749	PF00397	0.678
DOC_WW_Pin1_4	751	756	PF00397	0.657
LIG_14-3-3_CanoR_1	203	208	PF00244	0.528
LIG_14-3-3_CanoR_1	251	255	PF00244	0.413
LIG_14-3-3_CanoR_1	517	525	PF00244	0.503
LIG_14-3-3_CanoR_1	691	696	PF00244	0.452
LIG_14-3-3_CanoR_1	95	100	PF00244	0.426
LIG_Actin_WH2_2	29	47	PF00022	0.531
LIG_AP2alpha_2	309	311	PF02296	0.388
LIG_AP2alpha_2	523	525	PF02296	0.505
LIG_APCC_ABBAyCdc20_2	280	286	PF00400	0.447
LIG_BIR_II_1	1	5	PF00653	0.657
LIG_Clathr_ClatBox_1	347	351	PF01394	0.350
LIG_Clathr_ClatBox_1	395	399	PF01394	0.532
LIG_deltaCOP1_diTrp_1	277	284	PF00928	0.507
LIG_deltaCOP1_diTrp_1	309	314	PF00928	0.395
LIG_DLG_GKlike_1	691	699	PF00625	0.412
LIG_FHA_1	105	111	PF00498	0.518
LIG_FHA_1	123	129	PF00498	0.294
LIG_FHA_1	328	334	PF00498	0.652
LIG_FHA_1	439	445	PF00498	0.615
LIG_FHA_1	466	472	PF00498	0.719
LIG_FHA_1	505	511	PF00498	0.415
LIG_FHA_1	68	74	PF00498	0.484
LIG_FHA_1	752	758	PF00498	0.720
LIG_FHA_1	787	793	PF00498	0.491
LIG_FHA_2	184	190	PF00498	0.783
LIG_FHA_2	244	250	PF00498	0.563
LIG_FHA_2	357	363	PF00498	0.510
LIG_FHA_2	480	486	PF00498	0.605
LIG_FHA_2	542	548	PF00498	0.548
LIG_IBAR_NPY_1	518	520	PF08397	0.590
LIG_Integrin_RGD_1	735	737	PF01839	0.629
LIG_LIR_Apic_2	782	786	PF02991	0.565
LIG_LIR_Gen_1	19	26	PF02991	0.530
LIG_LIR_Gen_1	213	222	PF02991	0.681
LIG_LIR_Gen_1	299	310	PF02991	0.532
LIG_LIR_Gen_1	30	39	PF02991	0.421
LIG_LIR_Gen_1	359	368	PF02991	0.522
LIG_LIR_Gen_1	475	483	PF02991	0.489
LIG_LIR_Gen_1	533	543	PF02991	0.402
LIG_LIR_Nem_3	19	23	PF02991	0.511
LIG_LIR_Nem_3	213	217	PF02991	0.549
LIG_LIR_Nem_3	25	29	PF02991	0.414
LIG_LIR_Nem_3	299	305	PF02991	0.546
LIG_LIR_Nem_3	30	36	PF02991	0.380
LIG_LIR_Nem_3	309	314	PF02991	0.418
LIG_LIR_Nem_3	359	364	PF02991	0.512
LIG_LIR_Nem_3	475	481	PF02991	0.457
LIG_LIR_Nem_3	522	528	PF02991	0.488
LIG_LIR_Nem_3	533	538	PF02991	0.472
LIG_PCNA_PIPBox_1	389	398	PF02747	0.521
LIG_PCNA_yPIPBox_3	389	397	PF02747	0.431
LIG_Pex14_1	136	140	PF04695	0.445
LIG_Pex14_1	268	272	PF04695	0.462
LIG_PTAP_UEV_1	572	577	PF05743	0.511
LIG_SH2_CRK	302	306	PF00017	0.582
LIG_SH2_CRK	76	80	PF00017	0.433
LIG_SH2_PTP2	535	538	PF00017	0.404
LIG_SH2_STAP1	210	214	PF00017	0.654
LIG_SH2_STAP1	302	306	PF00017	0.582
LIG_SH2_STAP1	719	723	PF00017	0.586
LIG_SH2_STAT5	140	143	PF00017	0.582
LIG_SH2_STAT5	514	517	PF00017	0.584
LIG_SH2_STAT5	520	523	PF00017	0.556
LIG_SH2_STAT5	535	538	PF00017	0.473
LIG_SH2_STAT5	542	545	PF00017	0.477
LIG_SH2_STAT5	688	691	PF00017	0.330
LIG_SH2_STAT5	81	84	PF00017	0.489
LIG_SH3_2	323	328	PF14604	0.634
LIG_SH3_3	120	126	PF00018	0.501
LIG_SH3_3	320	326	PF00018	0.518
LIG_SH3_3	570	576	PF00018	0.470
LIG_SH3_3	649	655	PF00018	0.528
LIG_SH3_3	738	744	PF00018	0.731
LIG_SH3_3	747	753	PF00018	0.667
LIG_SH3_4	337	344	PF00018	0.360
LIG_SUMO_SIM_anti_2	375	380	PF11976	0.442
LIG_SUMO_SIM_par_1	236	242	PF11976	0.550
LIG_SUMO_SIM_par_1	431	438	PF11976	0.503
LIG_SUMO_SIM_par_1	467	473	PF11976	0.728
LIG_TRAF2_1	8	11	PF00917	0.617
LIG_TYR_ITIM	74	79	PF00017	0.447
LIG_UBA3_1	335	341	PF00899	0.575
MOD_CDC14_SPxK_1	194	197	PF00782	0.529
MOD_CDC14_SPxK_1	749	752	PF00782	0.648
MOD_CDK_SPK_2	451	456	PF00069	0.666
MOD_CDK_SPxK_1	191	197	PF00069	0.538
MOD_CDK_SPxK_1	746	752	PF00069	0.651
MOD_CDK_SPxxK_3	451	458	PF00069	0.724
MOD_CDK_SPxxK_3	582	589	PF00069	0.621
MOD_CK1_1	127	133	PF00069	0.553
MOD_CK1_1	169	175	PF00069	0.666
MOD_CK1_1	2	8	PF00069	0.653
MOD_CK1_1	413	419	PF00069	0.734
MOD_CK1_1	438	444	PF00069	0.512
MOD_CK1_1	622	628	PF00069	0.599
MOD_CK1_1	725	731	PF00069	0.631
MOD_CK1_1	746	752	PF00069	0.721
MOD_CK1_1	779	785	PF00069	0.692
MOD_CK2_1	171	177	PF00069	0.702
MOD_CK2_1	243	249	PF00069	0.577
MOD_CK2_1	356	362	PF00069	0.557
MOD_CK2_1	479	485	PF00069	0.476
MOD_CK2_1	5	11	PF00069	0.628
MOD_CK2_1	566	572	PF00069	0.553
MOD_GlcNHglycan	168	171	PF01048	0.601
MOD_GlcNHglycan	200	203	PF01048	0.667
MOD_GlcNHglycan	298	301	PF01048	0.561
MOD_GlcNHglycan	39	42	PF01048	0.543
MOD_GlcNHglycan	572	576	PF01048	0.517
MOD_GlcNHglycan	609	613	PF01048	0.390
MOD_GlcNHglycan	623	627	PF01048	0.431
MOD_GlcNHglycan	778	781	PF01048	0.614
MOD_GSK3_1	1	8	PF00069	0.713
MOD_GSK3_1	166	173	PF00069	0.583
MOD_GSK3_1	183	190	PF00069	0.752
MOD_GSK3_1	243	250	PF00069	0.545
MOD_GSK3_1	387	394	PF00069	0.482
MOD_GSK3_1	412	419	PF00069	0.686
MOD_GSK3_1	434	441	PF00069	0.580
MOD_GSK3_1	465	472	PF00069	0.717
MOD_GSK3_1	591	598	PF00069	0.488
MOD_GSK3_1	618	625	PF00069	0.529
MOD_GSK3_1	723	730	PF00069	0.515
MOD_GSK3_1	742	749	PF00069	0.714
MOD_GSK3_1	759	766	PF00069	0.411
MOD_N-GLC_1	284	289	PF02516	0.434
MOD_N-GLC_1	426	431	PF02516	0.692
MOD_N-GLC_1	479	484	PF02516	0.599
MOD_N-GLC_1	566	571	PF02516	0.525
MOD_N-GLC_2	690	692	PF02516	0.330
MOD_NEK2_1	198	203	PF00069	0.699
MOD_NEK2_1	243	248	PF00069	0.564
MOD_NEK2_1	284	289	PF00069	0.457
MOD_NEK2_1	293	298	PF00069	0.512
MOD_NEK2_1	426	431	PF00069	0.568
MOD_NEK2_1	504	509	PF00069	0.477
MOD_NEK2_1	566	571	PF00069	0.505
MOD_NEK2_1	577	582	PF00069	0.484
MOD_NEK2_1	711	716	PF00069	0.394
MOD_NEK2_2	327	332	PF00069	0.515
MOD_PK_1	95	101	PF00069	0.478
MOD_PKA_1	37	43	PF00069	0.546
MOD_PKA_2	166	172	PF00069	0.656
MOD_PKA_2	250	256	PF00069	0.531
MOD_PKA_2	37	43	PF00069	0.546
MOD_PKA_2	413	419	PF00069	0.645
MOD_PKA_2	435	441	PF00069	0.623
MOD_PKA_2	696	702	PF00069	0.481
MOD_Plk_1	284	290	PF00069	0.503
MOD_Plk_1	474	480	PF00069	0.486
MOD_Plk_1	566	572	PF00069	0.534
MOD_Plk_1	622	628	PF00069	0.492
MOD_Plk_4	260	266	PF00069	0.367
MOD_Plk_4	377	383	PF00069	0.416
MOD_Plk_4	391	397	PF00069	0.473
MOD_Plk_4	531	537	PF00069	0.397
MOD_Plk_4	566	572	PF00069	0.512
MOD_Plk_4	779	785	PF00069	0.620
MOD_ProDKin_1	122	128	PF00069	0.495
MOD_ProDKin_1	191	197	PF00069	0.697
MOD_ProDKin_1	370	376	PF00069	0.422
MOD_ProDKin_1	451	457	PF00069	0.658
MOD_ProDKin_1	5	11	PF00069	0.603
MOD_ProDKin_1	582	588	PF00069	0.510
MOD_ProDKin_1	744	750	PF00069	0.675
MOD_ProDKin_1	751	757	PF00069	0.657
TRG_DiLeu_BaEn_1	213	218	PF01217	0.643
TRG_DiLeu_BaEn_1	53	58	PF01217	0.449
TRG_DiLeu_BaEn_2	398	404	PF01217	0.529
TRG_DiLeu_BaLyEn_6	655	660	PF01217	0.535
TRG_DiLeu_BaLyEn_6	69	74	PF01217	0.539
TRG_ENDOCYTIC_2	140	143	PF00928	0.463
TRG_ENDOCYTIC_2	26	29	PF00928	0.462
TRG_ENDOCYTIC_2	302	305	PF00928	0.563
TRG_ENDOCYTIC_2	361	364	PF00928	0.474
TRG_ENDOCYTIC_2	535	538	PF00928	0.404
TRG_ENDOCYTIC_2	76	79	PF00928	0.408
TRG_ER_diArg_1	279	281	PF00400	0.455
TRG_ER_diArg_1	430	432	PF00400	0.561
TRG_ER_diArg_1	457	460	PF00400	0.760
TRG_ER_diArg_1	500	502	PF00400	0.489
TRG_ER_diArg_1	71	73	PF00400	0.556
TRG_NES_CRM1_1	386	399	PF08389	0.524
TRG_NES_CRM1_1	560	572	PF08389	0.539
TRG_Pf-PMV_PEXEL_1	163	168	PF00026	0.574
TRG_Pf-PMV_PEXEL_1	281	286	PF00026	0.481

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IJU9	Leptomonas seymouri	47%	100%
A0A0S4JDR0	Bodo saltans	26%	86%
A0A1X0NY20	Trypanosomatidae	30%	100%
A0A422NEI4	Trypanosoma rangeli	30%	100%
A4HYM1	Leishmania infantum	99%	100%
C9ZHT5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	100%
E9AI85	Leishmania braziliensis	74%	98%
E9AUH0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
Q4QD14	Leishmania major	93%	100%