Multisite-specific tRNA:(Cytosine-C(5))-methyltransferase, putative

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Multisite-specific tRNA:(cytosine-C(5))-methyltransferase, putative

Species:

Leishmania donovani

UniProt:

A0A3S7WV22_LEIDO

TriTrypDb:

LdBPK_181120.1 , LdCL_180016400 , LDHU3_18.1420

Length:

848

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005730	nucleolus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A0A3S7WV22

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7WV22

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	13
GO:0006139	nucleobase-containing compound metabolic process	3	13
GO:0006725	cellular aromatic compound metabolic process	3	13
GO:0006807	nitrogen compound metabolic process	2	13
GO:0008152	metabolic process	1	13
GO:0009451	RNA modification	5	13
GO:0009987	cellular process	1	13
GO:0016070	RNA metabolic process	5	13
GO:0032259	methylation	2	13
GO:0034641	cellular nitrogen compound metabolic process	3	13
GO:0043170	macromolecule metabolic process	3	13
GO:0043412	macromolecule modification	4	13
GO:0043414	macromolecule methylation	3	13
GO:0044237	cellular metabolic process	2	13
GO:0044238	primary metabolic process	2	13
GO:0044260	obsolete cellular macromolecule metabolic process	3	13
GO:0046483	heterocycle metabolic process	3	13
GO:0071704	organic substance metabolic process	2	13
GO:0090304	nucleic acid metabolic process	4	13
GO:1901360	organic cyclic compound metabolic process	3	13

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	13
GO:0003723	RNA binding	4	13
GO:0003824	catalytic activity	1	13
GO:0005488	binding	1	13
GO:0008168	methyltransferase activity	4	13
GO:0016740	transferase activity	2	13
GO:0016741	transferase activity, transferring one-carbon groups	3	13
GO:0097159	organic cyclic compound binding	2	13
GO:1901363	heterocyclic compound binding	2	13

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	688	694	PF00089	0.486
CLV_NRD_NRD_1	133	135	PF00675	0.588
CLV_NRD_NRD_1	197	199	PF00675	0.709
CLV_NRD_NRD_1	3	5	PF00675	0.635
CLV_NRD_NRD_1	410	412	PF00675	0.261
CLV_NRD_NRD_1	538	540	PF00675	0.280
CLV_NRD_NRD_1	586	588	PF00675	0.426
CLV_NRD_NRD_1	620	622	PF00675	0.418
CLV_NRD_NRD_1	686	688	PF00675	0.435
CLV_NRD_NRD_1	7	9	PF00675	0.559
CLV_NRD_NRD_1	99	101	PF00675	0.500
CLV_PCSK_FUR_1	356	360	PF00082	0.291
CLV_PCSK_KEX2_1	132	134	PF00082	0.594
CLV_PCSK_KEX2_1	2	4	PF00082	0.646
CLV_PCSK_KEX2_1	270	272	PF00082	0.507
CLV_PCSK_KEX2_1	358	360	PF00082	0.286
CLV_PCSK_KEX2_1	586	588	PF00082	0.532
CLV_PCSK_KEX2_1	620	622	PF00082	0.418
CLV_PCSK_KEX2_1	686	688	PF00082	0.485
CLV_PCSK_KEX2_1	7	9	PF00082	0.531
CLV_PCSK_KEX2_1	99	101	PF00082	0.500
CLV_PCSK_PC1ET2_1	2	4	PF00082	0.683
CLV_PCSK_PC1ET2_1	270	272	PF00082	0.540
CLV_PCSK_PC1ET2_1	358	360	PF00082	0.286
CLV_PCSK_PC7_1	3	9	PF00082	0.639
CLV_PCSK_PC7_1	682	688	PF00082	0.537
CLV_PCSK_SKI1_1	235	239	PF00082	0.499
CLV_PCSK_SKI1_1	336	340	PF00082	0.362
CLV_PCSK_SKI1_1	433	437	PF00082	0.300
CLV_PCSK_SKI1_1	539	543	PF00082	0.516
CLV_PCSK_SKI1_1	621	625	PF00082	0.393
CLV_PCSK_SKI1_1	696	700	PF00082	0.480
CLV_PCSK_SKI1_1	715	719	PF00082	0.490
DEG_Nend_UBRbox_1	1	4	PF02207	0.687
DEG_ODPH_VHL_1	679	692	PF01847	0.506
DEG_SIAH_1	730	738	PF03145	0.640
DEG_SPOP_SBC_1	597	601	PF00917	0.550
DOC_CKS1_1	212	217	PF01111	0.499
DOC_CKS1_1	237	242	PF01111	0.560
DOC_CKS1_1	624	629	PF01111	0.489
DOC_CYCLIN_RxL_1	433	445	PF00134	0.504
DOC_CYCLIN_RxL_1	682	693	PF00134	0.533
DOC_CYCLIN_yCln2_LP_2	564	570	PF00134	0.523
DOC_CYCLIN_yCln2_LP_2	624	630	PF00134	0.490
DOC_MAPK_gen_1	561	570	PF00069	0.536
DOC_MAPK_gen_1	637	646	PF00069	0.426
DOC_MAPK_HePTP_8	634	646	PF00069	0.535
DOC_MAPK_MEF2A_6	637	646	PF00069	0.426
DOC_PP1_RVXF_1	684	691	PF00149	0.559
DOC_PP2B_LxvP_1	50	53	PF13499	0.563
DOC_PP2B_LxvP_1	564	567	PF13499	0.481
DOC_PP4_FxxP_1	678	681	PF00568	0.525
DOC_USP7_MATH_1	137	141	PF00917	0.540
DOC_USP7_MATH_1	167	171	PF00917	0.589
DOC_USP7_MATH_1	251	255	PF00917	0.523
DOC_USP7_MATH_1	383	387	PF00917	0.570
DOC_USP7_MATH_1	56	60	PF00917	0.784
DOC_USP7_MATH_1	597	601	PF00917	0.598
DOC_USP7_UBL2_3	344	348	PF12436	0.480
DOC_WW_Pin1_4	211	216	PF00397	0.418
DOC_WW_Pin1_4	236	241	PF00397	0.536
DOC_WW_Pin1_4	497	502	PF00397	0.480
DOC_WW_Pin1_4	623	628	PF00397	0.492
LIG_14-3-3_CanoR_1	100	109	PF00244	0.459
LIG_14-3-3_CanoR_1	221	229	PF00244	0.480
LIG_14-3-3_CanoR_1	235	240	PF00244	0.500
LIG_14-3-3_CanoR_1	346	354	PF00244	0.480
LIG_14-3-3_CanoR_1	414	424	PF00244	0.508
LIG_14-3-3_CanoR_1	621	627	PF00244	0.504
LIG_14-3-3_CanoR_1	682	690	PF00244	0.564
LIG_14-3-3_CanoR_1	824	828	PF00244	0.614
LIG_APCC_ABBA_1	523	528	PF00400	0.480
LIG_BRCT_BRCA1_1	674	678	PF00533	0.499
LIG_deltaCOP1_diTrp_1	202	208	PF00928	0.438
LIG_deltaCOP1_diTrp_1	571	581	PF00928	0.404
LIG_Dynein_DLC8_1	346	352	PF01221	0.520
LIG_FAT_LD_1	75	83	PF03623	0.537
LIG_FHA_1	144	150	PF00498	0.569
LIG_FHA_1	18	24	PF00498	0.552
LIG_FHA_1	217	223	PF00498	0.390
LIG_FHA_1	232	238	PF00498	0.418
LIG_FHA_1	253	259	PF00498	0.520
LIG_FHA_1	348	354	PF00498	0.489
LIG_FHA_1	443	449	PF00498	0.547
LIG_FHA_1	624	630	PF00498	0.428
LIG_FHA_1	762	768	PF00498	0.559
LIG_FHA_1	96	102	PF00498	0.586
LIG_FHA_2	236	242	PF00498	0.546
LIG_FHA_2	304	310	PF00498	0.539
LIG_FHA_2	366	372	PF00498	0.491
LIG_FHA_2	42	48	PF00498	0.689
LIG_FHA_2	751	757	PF00498	0.705
LIG_LIR_Apic_2	545	549	PF02991	0.493
LIG_LIR_Apic_2	636	642	PF02991	0.434
LIG_LIR_Apic_2	675	681	PF02991	0.471
LIG_LIR_Gen_1	156	162	PF02991	0.467
LIG_LIR_Gen_1	202	212	PF02991	0.410
LIG_LIR_Gen_1	286	295	PF02991	0.428
LIG_LIR_Gen_1	39	47	PF02991	0.590
LIG_LIR_Gen_1	392	403	PF02991	0.471
LIG_LIR_Gen_1	572	582	PF02991	0.431
LIG_LIR_Gen_1	89	97	PF02991	0.583
LIG_LIR_Nem_3	156	161	PF02991	0.458
LIG_LIR_Nem_3	202	208	PF02991	0.448
LIG_LIR_Nem_3	286	290	PF02991	0.436
LIG_LIR_Nem_3	39	43	PF02991	0.534
LIG_LIR_Nem_3	392	398	PF02991	0.471
LIG_LIR_Nem_3	572	578	PF02991	0.394
LIG_LIR_Nem_3	579	584	PF02991	0.400
LIG_LIR_Nem_3	589	595	PF02991	0.422
LIG_LIR_Nem_3	712	717	PF02991	0.544
LIG_LIR_Nem_3	815	819	PF02991	0.500
LIG_LIR_Nem_3	89	95	PF02991	0.578
LIG_MYND_1	509	513	PF01753	0.577
LIG_NRBOX	698	704	PF00104	0.534
LIG_NRBOX	74	80	PF00104	0.532
LIG_NRBOX	740	746	PF00104	0.680
LIG_Pex14_1	375	379	PF04695	0.491
LIG_Pex14_1	420	424	PF04695	0.480
LIG_Pex14_2	534	538	PF04695	0.475
LIG_SH2_CRK	360	364	PF00017	0.504
LIG_SH2_CRK	639	643	PF00017	0.474
LIG_SH2_STAT5	274	277	PF00017	0.429
LIG_SH2_STAT5	35	38	PF00017	0.488
LIG_SH2_STAT5	379	382	PF00017	0.562
LIG_SH2_STAT5	424	427	PF00017	0.480
LIG_SH2_STAT5	709	712	PF00017	0.656
LIG_SH3_3	242	248	PF00018	0.465
LIG_SH3_3	370	376	PF00018	0.502
LIG_SH3_3	506	512	PF00018	0.532
LIG_SH3_3	538	544	PF00018	0.549
LIG_SUMO_SIM_anti_2	474	480	PF11976	0.480
LIG_SUMO_SIM_anti_2	755	765	PF11976	0.661
LIG_SUMO_SIM_par_1	124	131	PF11976	0.585
LIG_TRAF2_1	286	289	PF00917	0.367
LIG_TRAF2_1	755	758	PF00917	0.694
LIG_TRAF2_1	840	843	PF00917	0.795
LIG_TRFH_1	610	614	PF08558	0.451
LIG_WRC_WIRS_1	578	583	PF05994	0.512
LIG_WRC_WIRS_1	813	818	PF05994	0.456
LIG_WW_1	512	515	PF00397	0.472
MOD_CK1_1	104	110	PF00069	0.428
MOD_CK1_1	121	127	PF00069	0.470
MOD_CK1_1	203	209	PF00069	0.492
MOD_CK1_1	211	217	PF00069	0.472
MOD_CK1_1	283	289	PF00069	0.378
MOD_CK1_1	415	421	PF00069	0.373
MOD_CK1_1	452	458	PF00069	0.320
MOD_CK1_1	599	605	PF00069	0.595
MOD_CK1_1	749	755	PF00069	0.692
MOD_CK2_1	183	189	PF00069	0.725
MOD_CK2_1	283	289	PF00069	0.369
MOD_CK2_1	383	389	PF00069	0.307
MOD_CK2_1	41	47	PF00069	0.626
MOD_CK2_1	70	76	PF00069	0.549
MOD_CK2_1	736	742	PF00069	0.692
MOD_CK2_1	752	758	PF00069	0.586
MOD_CK2_1	762	768	PF00069	0.558
MOD_GlcNHglycan	123	126	PF01048	0.592
MOD_GlcNHglycan	139	142	PF01048	0.656
MOD_GlcNHglycan	169	172	PF01048	0.575
MOD_GlcNHglycan	191	194	PF01048	0.769
MOD_GlcNHglycan	204	208	PF01048	0.457
MOD_GlcNHglycan	255	258	PF01048	0.527
MOD_GlcNHglycan	282	285	PF01048	0.398
MOD_GlcNHglycan	291	294	PF01048	0.363
MOD_GlcNHglycan	326	329	PF01048	0.415
MOD_GlcNHglycan	330	333	PF01048	0.383
MOD_GlcNHglycan	404	407	PF01048	0.323
MOD_GlcNHglycan	420	423	PF01048	0.323
MOD_GlcNHglycan	493	496	PF01048	0.396
MOD_GlcNHglycan	54	57	PF01048	0.756
MOD_GlcNHglycan	58	61	PF01048	0.719
MOD_GlcNHglycan	601	604	PF01048	0.598
MOD_GlcNHglycan	674	677	PF01048	0.380
MOD_GlcNHglycan	72	75	PF01048	0.370
MOD_GlcNHglycan	721	724	PF01048	0.511
MOD_GlcNHglycan	791	794	PF01048	0.409
MOD_GlcNHglycan	795	798	PF01048	0.473
MOD_GlcNHglycan	833	836	PF01048	0.747
MOD_GSK3_1	132	139	PF00069	0.693
MOD_GSK3_1	231	238	PF00069	0.515
MOD_GSK3_1	253	260	PF00069	0.535
MOD_GSK3_1	261	268	PF00069	0.509
MOD_GSK3_1	324	331	PF00069	0.411
MOD_GSK3_1	381	388	PF00069	0.452
MOD_GSK3_1	402	409	PF00069	0.323
MOD_GSK3_1	412	419	PF00069	0.323
MOD_GSK3_1	420	427	PF00069	0.323
MOD_GSK3_1	52	59	PF00069	0.755
MOD_GSK3_1	595	602	PF00069	0.551
MOD_GSK3_1	746	753	PF00069	0.651
MOD_GSK3_1	775	782	PF00069	0.502
MOD_GSK3_1	789	796	PF00069	0.388
MOD_LATS_1	130	136	PF00433	0.699
MOD_N-GLC_1	183	188	PF02516	0.727
MOD_N-GLC_1	194	199	PF02516	0.680
MOD_N-GLC_1	384	389	PF02516	0.258
MOD_N-GLC_1	576	581	PF02516	0.429
MOD_N-GLC_1	829	834	PF02516	0.736
MOD_NEK2_1	118	123	PF00069	0.551
MOD_NEK2_1	143	148	PF00069	0.589
MOD_NEK2_1	208	213	PF00069	0.464
MOD_NEK2_1	442	447	PF00069	0.379
MOD_NEK2_1	449	454	PF00069	0.299
MOD_NEK2_1	690	695	PF00069	0.541
MOD_NEK2_1	762	767	PF00069	0.594
MOD_NEK2_1	791	796	PF00069	0.409
MOD_NEK2_2	406	411	PF00069	0.323
MOD_NEK2_2	812	817	PF00069	0.499
MOD_PIKK_1	101	107	PF00454	0.486
MOD_PIKK_1	118	124	PF00454	0.425
MOD_PIKK_1	347	353	PF00454	0.336
MOD_PIKK_1	41	47	PF00454	0.640
MOD_PIKK_1	428	434	PF00454	0.336
MOD_PIKK_1	762	768	PF00454	0.625
MOD_PK_1	412	418	PF00069	0.418
MOD_PK_1	77	83	PF00069	0.526
MOD_PKA_1	132	138	PF00069	0.710
MOD_PKA_2	132	138	PF00069	0.710
MOD_PKA_2	153	159	PF00069	0.544
MOD_PKA_2	681	687	PF00069	0.534
MOD_PKA_2	690	696	PF00069	0.472
MOD_PKA_2	823	829	PF00069	0.711
MOD_PKA_2	95	101	PF00069	0.569
MOD_Plk_1	143	149	PF00069	0.567
MOD_Plk_1	203	209	PF00069	0.433
MOD_Plk_1	412	418	PF00069	0.418
MOD_Plk_1	576	582	PF00069	0.464
MOD_Plk_1	750	756	PF00069	0.641
MOD_Plk_4	104	110	PF00069	0.545
MOD_Plk_4	303	309	PF00069	0.344
MOD_Plk_4	321	327	PF00069	0.370
MOD_Plk_4	420	426	PF00069	0.352
MOD_Plk_4	547	553	PF00069	0.600
MOD_Plk_4	577	583	PF00069	0.452
MOD_Plk_4	674	680	PF00069	0.413
MOD_Plk_4	77	83	PF00069	0.518
MOD_Plk_4	812	818	PF00069	0.477
MOD_Plk_4	823	829	PF00069	0.665
MOD_ProDKin_1	211	217	PF00069	0.419
MOD_ProDKin_1	236	242	PF00069	0.529
MOD_ProDKin_1	497	503	PF00069	0.335
MOD_ProDKin_1	623	629	PF00069	0.489
MOD_SUMO_rev_2	655	662	PF00179	0.455
TRG_DiLeu_BaEn_1	758	763	PF01217	0.693
TRG_DiLeu_BaEn_1	823	828	PF01217	0.532
TRG_DiLeu_BaEn_2	203	209	PF01217	0.468
TRG_DiLeu_BaEn_2	673	679	PF01217	0.499
TRG_DiLeu_LyEn_5	145	150	PF01217	0.463
TRG_ER_diArg_1	132	134	PF00400	0.594
TRG_ER_diArg_1	619	621	PF00400	0.443
TRG_ER_diArg_1	634	637	PF00400	0.519
TRG_ER_diArg_1	686	688	PF00400	0.489
TRG_ER_diArg_1	7	10	PF00400	0.595
TRG_ER_diArg_1	99	101	PF00400	0.519
TRG_NES_CRM1_1	13	29	PF08389	0.492
TRG_Pf-PMV_PEXEL_1	397	401	PF00026	0.370
TRG_Pf-PMV_PEXEL_1	696	700	PF00026	0.558

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8H1	Leptomonas seymouri	72%	98%
A0A0S4KJ12	Bodo saltans	42%	100%
A0A1X0P6Y3	Trypanosomatidae	54%	100%
A0A3R7M922	Trypanosoma rangeli	52%	100%
A4H9M1	Leishmania braziliensis	83%	99%
A4HXY5	Leishmania infantum	99%	100%
D0A386	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	25%	100%
D0A5C2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	50%	100%
E9ARP8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
Q4QDT1	Leishmania major	94%	100%
V5DFH6	Trypanosoma cruzi	52%	100%