Proline--tRNA ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

prolyl-tRNA synthetase, putative (fragment)

Species:

Leishmania donovani

UniProt:

A0A3S7WV15_LEIDO

TriTrypDb:

LdBPK_181230.1 , LdCL_180017500 , LDHU3_18.1550

Length:

724

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 28

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 6

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 15
NetGPI	no	yes: 0, no: 15

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	16
GO:0110165	cellular anatomical entity	1	16
GO:0017101	aminoacyl-tRNA synthetase multienzyme complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0140535	intracellular protein-containing complex	2	2
GO:1902494	catalytic complex	2	2
GO:0016020	membrane	2	1

Expansion

Sequence features

A0A3S7WV15

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7WV15

PDB structure(s): 5xil_A

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	16
GO:0006139	nucleobase-containing compound metabolic process	3	16
GO:0006399	tRNA metabolic process	7	16
GO:0006418	tRNA aminoacylation for protein translation	6	16
GO:0006433	prolyl-tRNA aminoacylation	7	16
GO:0006520	amino acid metabolic process	3	16
GO:0006725	cellular aromatic compound metabolic process	3	16
GO:0006807	nitrogen compound metabolic process	2	16
GO:0008152	metabolic process	1	16
GO:0009987	cellular process	1	16
GO:0016070	RNA metabolic process	5	16
GO:0019752	carboxylic acid metabolic process	5	16
GO:0034641	cellular nitrogen compound metabolic process	3	16
GO:0034660	ncRNA metabolic process	6	16
GO:0043038	amino acid activation	4	16
GO:0043039	tRNA aminoacylation	5	16
GO:0043170	macromolecule metabolic process	3	16
GO:0043436	oxoacid metabolic process	4	16
GO:0044237	cellular metabolic process	2	16
GO:0044238	primary metabolic process	2	16
GO:0044281	small molecule metabolic process	2	16
GO:0046483	heterocycle metabolic process	3	16
GO:0071704	organic substance metabolic process	2	16
GO:0090304	nucleic acid metabolic process	4	16
GO:1901360	organic cyclic compound metabolic process	3	16
GO:1901564	organonitrogen compound metabolic process	3	16

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	16
GO:0002161	aminoacyl-tRNA editing activity	5	15
GO:0003824	catalytic activity	1	16
GO:0004812	aminoacyl-tRNA ligase activity	4	16
GO:0004827	proline-tRNA ligase activity	5	16
GO:0005488	binding	1	16
GO:0005524	ATP binding	5	16
GO:0016787	hydrolase activity	2	15
GO:0016788	hydrolase activity, acting on ester bonds	3	15
GO:0016874	ligase activity	2	16
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	16
GO:0017076	purine nucleotide binding	4	16
GO:0030554	adenyl nucleotide binding	5	16
GO:0032553	ribonucleotide binding	3	16
GO:0032555	purine ribonucleotide binding	4	16
GO:0032559	adenyl ribonucleotide binding	5	16
GO:0035639	purine ribonucleoside triphosphate binding	4	16
GO:0036094	small molecule binding	2	16
GO:0043167	ion binding	2	16
GO:0043168	anion binding	3	16
GO:0052689	carboxylic ester hydrolase activity	4	15
GO:0097159	organic cyclic compound binding	2	16
GO:0097367	carbohydrate derivative binding	2	16
GO:0140098	catalytic activity, acting on RNA	3	16
GO:0140101	catalytic activity, acting on a tRNA	4	16
GO:0140640	catalytic activity, acting on a nucleic acid	2	16
GO:1901265	nucleoside phosphate binding	3	16
GO:1901363	heterocyclic compound binding	2	16

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	545	549	PF00656	0.472
CLV_NRD_NRD_1	409	411	PF00675	0.347
CLV_NRD_NRD_1	529	531	PF00675	0.250
CLV_NRD_NRD_1	58	60	PF00675	0.166
CLV_NRD_NRD_1	640	642	PF00675	0.308
CLV_PCSK_KEX2_1	118	120	PF00082	0.273
CLV_PCSK_KEX2_1	220	222	PF00082	0.414
CLV_PCSK_KEX2_1	529	531	PF00082	0.309
CLV_PCSK_PC1ET2_1	118	120	PF00082	0.273
CLV_PCSK_PC1ET2_1	220	222	PF00082	0.414
CLV_PCSK_SKI1_1	119	123	PF00082	0.278
CLV_PCSK_SKI1_1	127	131	PF00082	0.288
CLV_PCSK_SKI1_1	366	370	PF00082	0.261
CLV_PCSK_SKI1_1	385	389	PF00082	0.261
CLV_PCSK_SKI1_1	52	56	PF00082	0.272
CLV_PCSK_SKI1_1	712	716	PF00082	0.291
DOC_CKS1_1	44	49	PF01111	0.485
DOC_CYCLIN_yCln2_LP_2	313	319	PF00134	0.313
DOC_MAPK_gen_1	118	125	PF00069	0.529
DOC_MAPK_gen_1	127	136	PF00069	0.544
DOC_MAPK_gen_1	142	151	PF00069	0.466
DOC_MAPK_gen_1	333	343	PF00069	0.461
DOC_MAPK_gen_1	57	66	PF00069	0.429
DOC_MAPK_MEF2A_6	118	125	PF00069	0.543
DOC_MAPK_MEF2A_6	336	345	PF00069	0.461
DOC_MAPK_MEF2A_6	479	487	PF00069	0.485
DOC_MAPK_MEF2A_6	562	570	PF00069	0.451
DOC_PP1_RVXF_1	164	171	PF00149	0.446
DOC_PP1_RVXF_1	84	91	PF00149	0.474
DOC_PP2B_LxvP_1	402	405	PF13499	0.451
DOC_PP2B_LxvP_1	689	692	PF13499	0.461
DOC_PP4_FxxP_1	356	359	PF00568	0.461
DOC_PP4_FxxP_1	44	47	PF00568	0.443
DOC_PP4_FxxP_1	457	460	PF00568	0.461
DOC_PP4_FxxP_1	693	696	PF00568	0.461
DOC_USP7_MATH_1	576	580	PF00917	0.563
DOC_USP7_MATH_1	589	593	PF00917	0.514
DOC_USP7_MATH_1	664	668	PF00917	0.461
DOC_USP7_MATH_2	92	98	PF00917	0.429
DOC_USP7_UBL2_3	708	712	PF12436	0.513
DOC_WW_Pin1_4	105	110	PF00397	0.472
DOC_WW_Pin1_4	129	134	PF00397	0.500
DOC_WW_Pin1_4	357	362	PF00397	0.472
DOC_WW_Pin1_4	43	48	PF00397	0.456
DOC_WW_Pin1_4	548	553	PF00397	0.461
DOC_WW_Pin1_4	596	601	PF00397	0.533
LIG_14-3-3_CanoR_1	119	124	PF00244	0.500
LIG_14-3-3_CanoR_1	320	329	PF00244	0.461
LIG_14-3-3_CanoR_1	408	417	PF00244	0.499
LIG_14-3-3_CanoR_1	499	509	PF00244	0.347
LIG_14-3-3_CanoR_1	590	594	PF00244	0.475
LIG_14-3-3_CanoR_1	626	636	PF00244	0.502
LIG_Actin_WH2_2	117	132	PF00022	0.522
LIG_APCC_ABBA_1	485	490	PF00400	0.395
LIG_BIR_II_1	1	5	PF00653	0.609
LIG_Clathr_ClatBox_1	148	152	PF01394	0.447
LIG_deltaCOP1_diTrp_1	367	377	PF00928	0.472
LIG_deltaCOP1_diTrp_1	631	635	PF00928	0.347
LIG_FHA_1	120	126	PF00498	0.526
LIG_FHA_1	358	364	PF00498	0.472
LIG_FHA_1	421	427	PF00498	0.461
LIG_FHA_1	44	50	PF00498	0.474
LIG_FHA_1	476	482	PF00498	0.461
LIG_FHA_1	501	507	PF00498	0.434
LIG_FHA_1	622	628	PF00498	0.425
LIG_FHA_1	686	692	PF00498	0.502
LIG_FHA_1	713	719	PF00498	0.547
LIG_FHA_1	83	89	PF00498	0.513
LIG_FHA_2	232	238	PF00498	0.342
LIG_FHA_2	309	315	PF00498	0.460
LIG_FHA_2	31	37	PF00498	0.472
LIG_FHA_2	410	416	PF00498	0.458
LIG_FHA_2	422	428	PF00498	0.467
LIG_FHA_2	514	520	PF00498	0.458
LIG_FHA_2	673	679	PF00498	0.493
LIG_FHA_2	70	76	PF00498	0.535
LIG_LIR_Apic_2	355	359	PF02991	0.461
LIG_LIR_Apic_2	454	460	PF02991	0.490
LIG_LIR_Gen_1	108	116	PF02991	0.506
LIG_LIR_Gen_1	226	236	PF02991	0.390
LIG_LIR_Gen_1	392	402	PF02991	0.472
LIG_LIR_Gen_1	630	639	PF02991	0.313
LIG_LIR_Gen_1	715	724	PF02991	0.523
LIG_LIR_LC3C_4	146	150	PF02991	0.429
LIG_LIR_Nem_3	108	113	PF02991	0.518
LIG_LIR_Nem_3	226	232	PF02991	0.385
LIG_LIR_Nem_3	240	244	PF02991	0.344
LIG_LIR_Nem_3	276	282	PF02991	0.377
LIG_LIR_Nem_3	325	331	PF02991	0.455
LIG_LIR_Nem_3	392	397	PF02991	0.472
LIG_LIR_Nem_3	630	635	PF02991	0.313
LIG_LIR_Nem_3	715	720	PF02991	0.523
LIG_NRBOX	11	17	PF00104	0.331
LIG_Pex14_2	449	453	PF04695	0.461
LIG_SH2_CRK	394	398	PF00017	0.461
LIG_SH2_NCK_1	394	398	PF00017	0.472
LIG_SH2_PTP2	229	232	PF00017	0.409
LIG_SH2_SRC	229	232	PF00017	0.409
LIG_SH2_STAP1	163	167	PF00017	0.371
LIG_SH2_STAP1	331	335	PF00017	0.461
LIG_SH2_STAP1	394	398	PF00017	0.463
LIG_SH2_STAP1	422	426	PF00017	0.461
LIG_SH2_STAT5	229	232	PF00017	0.367
LIG_SH2_STAT5	248	251	PF00017	0.249
LIG_SH2_STAT5	256	259	PF00017	0.340
LIG_SH2_STAT5	278	281	PF00017	0.375
LIG_SH2_STAT5	422	425	PF00017	0.461
LIG_SH3_3	134	140	PF00018	0.443
LIG_SH3_3	323	329	PF00018	0.472
LIG_SH3_3	492	498	PF00018	0.360
LIG_SH3_3	693	699	PF00018	0.482
LIG_SUMO_SIM_anti_2	11	18	PF11976	0.425
LIG_SUMO_SIM_anti_2	386	392	PF11976	0.522
LIG_SUMO_SIM_anti_2	502	509	PF11976	0.432
LIG_SUMO_SIM_anti_2	62	67	PF11976	0.430
LIG_SUMO_SIM_anti_2	97	104	PF11976	0.535
LIG_SUMO_SIM_par_1	121	126	PF11976	0.542
LIG_SUMO_SIM_par_1	386	392	PF11976	0.522
LIG_SUMO_SIM_par_1	62	67	PF11976	0.444
LIG_TRAF2_1	33	36	PF00917	0.500
LIG_TRAF2_1	516	519	PF00917	0.503
LIG_TRAF2_1	520	523	PF00917	0.499
LIG_TRAF2_1	663	666	PF00917	0.465
LIG_TRFH_1	136	140	PF08558	0.447
LIG_TYR_ITIM	239	244	PF00017	0.383
LIG_UBA3_1	112	118	PF00899	0.510
LIG_UBA3_1	12	17	PF00899	0.492
LIG_WRC_WIRS_1	426	431	PF05994	0.563
LIG_WW_3	496	500	PF00397	0.371
MOD_CDK_SPxxK_3	357	364	PF00069	0.472
MOD_CDK_SPxxK_3	548	555	PF00069	0.461
MOD_CK1_1	207	213	PF00069	0.640
MOD_CK1_1	357	363	PF00069	0.472
MOD_CK2_1	231	237	PF00069	0.348
MOD_CK2_1	24	30	PF00069	0.444
MOD_CK2_1	308	314	PF00069	0.439
MOD_CK2_1	393	399	PF00069	0.544
MOD_CK2_1	409	415	PF00069	0.400
MOD_CK2_1	421	427	PF00069	0.467
MOD_CK2_1	513	519	PF00069	0.461
MOD_CK2_1	660	666	PF00069	0.461
MOD_Cter_Amidation	408	411	PF01082	0.272
MOD_GlcNHglycan	1	4	PF01048	0.461
MOD_GlcNHglycan	173	176	PF01048	0.575
MOD_GlcNHglycan	206	209	PF01048	0.630
MOD_GlcNHglycan	225	228	PF01048	0.264
MOD_GlcNHglycan	243	247	PF01048	0.235
MOD_GlcNHglycan	586	589	PF01048	0.341
MOD_GlcNHglycan	662	665	PF01048	0.261
MOD_GSK3_1	421	428	PF00069	0.478
MOD_GSK3_1	470	477	PF00069	0.461
MOD_GSK3_1	510	517	PF00069	0.485
MOD_GSK3_1	64	71	PF00069	0.485
MOD_GSK3_1	660	667	PF00069	0.461
MOD_GSK3_1	708	715	PF00069	0.547
MOD_N-GLC_1	119	124	PF02516	0.304
MOD_N-GLC_1	223	228	PF02516	0.455
MOD_N-GLC_1	548	553	PF02516	0.261
MOD_NEK2_1	281	286	PF00069	0.390
MOD_NEK2_1	524	529	PF00069	0.564
MOD_NEK2_1	55	60	PF00069	0.466
MOD_NEK2_2	393	398	PF00069	0.518
MOD_PIKK_1	500	506	PF00454	0.480
MOD_PKA_1	660	666	PF00069	0.461
MOD_PKA_2	319	325	PF00069	0.451
MOD_PKA_2	409	415	PF00069	0.547
MOD_PKA_2	584	590	PF00069	0.472
MOD_PKA_2	627	633	PF00069	0.513
MOD_Plk_1	119	125	PF00069	0.528
MOD_Plk_1	143	149	PF00069	0.465
MOD_Plk_1	223	229	PF00069	0.432
MOD_Plk_1	463	469	PF00069	0.502
MOD_Plk_1	576	582	PF00069	0.559
MOD_Plk_2-3	30	36	PF00069	0.485
MOD_Plk_4	119	125	PF00069	0.529
MOD_Plk_4	144	150	PF00069	0.463
MOD_Plk_4	322	328	PF00069	0.472
MOD_Plk_4	393	399	PF00069	0.518
MOD_Plk_4	425	431	PF00069	0.485
MOD_Plk_4	463	469	PF00069	0.474
MOD_Plk_4	589	595	PF00069	0.501
MOD_Plk_4	713	719	PF00069	0.547
MOD_ProDKin_1	105	111	PF00069	0.472
MOD_ProDKin_1	129	135	PF00069	0.500
MOD_ProDKin_1	357	363	PF00069	0.472
MOD_ProDKin_1	43	49	PF00069	0.456
MOD_ProDKin_1	548	554	PF00069	0.461
MOD_ProDKin_1	596	602	PF00069	0.533
MOD_SUMO_for_1	219	222	PF00179	0.400
MOD_SUMO_for_1	378	381	PF00179	0.547
MOD_SUMO_rev_2	207	215	PF00179	0.565
MOD_SUMO_rev_2	662	670	PF00179	0.448
TRG_DiLeu_BaEn_1	11	16	PF01217	0.478
TRG_DiLeu_BaEn_2	630	636	PF01217	0.434
TRG_ENDOCYTIC_2	165	168	PF00928	0.332
TRG_ENDOCYTIC_2	229	232	PF00928	0.352
TRG_ENDOCYTIC_2	241	244	PF00928	0.385
TRG_ENDOCYTIC_2	278	281	PF00928	0.375
TRG_ENDOCYTIC_2	394	397	PF00928	0.461
TRG_ENDOCYTIC_2	398	401	PF00928	0.461
TRG_ER_diArg_1	363	366	PF00400	0.461
TRG_ER_diArg_1	407	410	PF00400	0.554
TRG_ER_diArg_1	529	531	PF00400	0.525
TRG_ER_diArg_1	626	629	PF00400	0.544
TRG_NES_CRM1_1	11	22	PF08389	0.406
TRG_Pf-PMV_PEXEL_1	127	131	PF00026	0.281
TRG_Pf-PMV_PEXEL_1	529	534	PF00026	0.304
TRG_Pf-PMV_PEXEL_1	573	577	PF00026	0.347

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HY85	Leptomonas seymouri	84%	100%
A0A0S4JN58	Bodo saltans	59%	88%
A0A1X0P793	Trypanosomatidae	67%	89%
A0A3R7KPX0	Trypanosoma rangeli	68%	85%
A0A3S7WV32	Leishmania donovani	89%	100%
A0M4Z3	Gramella forsetii (strain KT0803)	35%	100%
A1RSR6	Pyrobaculum islandicum (strain DSM 4184 / JCM 9189 / GEO3)	29%	100%
A3MV50	Pyrobaculum calidifontis (strain DSM 21063 / JCM 11548 / VA1)	30%	100%
A4H9N1	Leishmania braziliensis	81%	99%
A4HXZ5	Leishmania infantum	89%	100%
A4HY00	Leishmania infantum	100%	100%
A5FA31	Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 / UW101)	35%	100%
A6L5I2	Phocaeicola vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / CCUG 4940 / NBRC 14291 / NCTC 11154)	37%	100%
A6LAP3	Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152)	35%	100%
B0R3A3	Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)	40%	100%
B2A5T8	Natranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF)	41%	100%
B2RJG1	Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / CIP 103683 / JCM 12257 / NCTC 11834 / 2561)	37%	100%
B3ER64	Amoebophilus asiaticus (strain 5a2)	37%	100%
B7J1X2	Borreliella burgdorferi (strain ZS7)	37%	100%
B9LUM0	Halorubrum lacusprofundi (strain ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34)	41%	100%
D0A5B2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	66%	90%
E9ARQ8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	85%	100%
E9ARQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	98%
O51363	Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)	37%	100%
O60155	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	46%	100%
P38708	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	48%	100%
Q0SNA5	Borreliella afzelii (strain PKo)	36%	100%
Q11Q45	Cytophaga hutchinsonii (strain ATCC 33406 / DSM 1761 / CIP 103989 / NBRC 15051 / NCIMB 9469 / D465)	34%	100%
Q18JG3	Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)	40%	100%
Q1CXV1	Myxococcus xanthus (strain DK1622)	36%	100%
Q1GTS7	Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256)	36%	100%
Q1J0W2	Deinococcus geothermalis (strain DSM 11300 / AG-3a)	36%	100%
Q2ILX2	Anaeromyxobacter dehalogenans (strain 2CP-C)	39%	100%
Q2RZJ2	Salinibacter ruber (strain DSM 13855 / M31)	36%	100%
Q2W136	Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)	36%	100%
Q4J8L4	Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)	31%	100%
Q4QDS0	Leishmania major	96%	100%
Q4QDS1	Leishmania major	87%	100%
Q5LCF0	Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / CCUG 4856 / JCM 11019 / NCTC 9343 / Onslow)	37%	100%
Q5V5H0	Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)	38%	100%
Q64TJ6	Bacteroides fragilis (strain YCH46)	37%	100%
Q661L6	Borrelia garinii subsp. bavariensis (strain ATCC BAA-2496 / DSM 23469 / PBi)	37%	100%
Q67PA6	Symbiobacterium thermophilum (strain T / IAM 14863)	41%	100%
Q6MIW2	Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIMB 9529 / HD100)	36%	100%
Q7MVS7	Porphyromonas gingivalis (strain ATCC BAA-308 / W83)	37%	100%
Q7X2P1	Sphingomonas elodea	36%	100%
Q81Z76	Bacillus anthracis	40%	100%
Q8A988	Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)	37%	100%
Q8I5R7	Plasmodium falciparum (isolate 3D7)	40%	97%
Q8SSD7	Encephalitozoon cuniculi (strain GB-M1)	49%	100%
Q971B5	Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)	29%	100%
Q9FYR6	Arabidopsis thaliana	37%	100%
Q9HS52	Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)	40%	100%
Q9M1R2	Arabidopsis thaliana	53%	100%
Q9RUW4	Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)	36%	100%
Q9Y9G0	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	32%	100%
V5AZ66	Trypanosoma cruzi	68%	89%