3-oxo-5-alpha-steroid 4-dehydrogenase-like protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

3-oxo-5-alpha-steroid 4-dehydrogenase-like protein

Species:

Leishmania donovani

UniProt:

A0A3S7WUK3_LEIDO

TriTrypDb:

LdBPK_171200.1 , LdCL_170018100 , LDHU3_17.1690

Length:

540

Annotations

LeishMANIAdb annotations

Related to other eukaryotic steroid 5 alpha reductases. Localization: ER (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	7
GO:0110165	cellular anatomical entity	1	7

Expansion

Sequence features

A0A3S7WUK3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7WUK3

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	7
GO:0008152	metabolic process	1	7
GO:0044238	primary metabolic process	2	7
GO:0071704	organic substance metabolic process	2	7

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	7
GO:0003865	3-oxo-5-alpha-steroid 4-dehydrogenase activity	5	6
GO:0016229	steroid dehydrogenase activity	3	6
GO:0016491	oxidoreductase activity	2	7
GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors	3	7
GO:0033765	steroid dehydrogenase activity, acting on the CH-CH group of donors	4	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	401	405	PF00656	0.374
CLV_C14_Caspase3-7	84	88	PF00656	0.672
CLV_MEL_PAP_1	135	141	PF00089	0.565
CLV_MEL_PAP_1	316	322	PF00089	0.315
CLV_NRD_NRD_1	358	360	PF00675	0.649
CLV_NRD_NRD_1	375	377	PF00675	0.696
CLV_NRD_NRD_1	51	53	PF00675	0.560
CLV_PCSK_FUR_1	356	360	PF00082	0.657
CLV_PCSK_KEX2_1	358	360	PF00082	0.649
CLV_PCSK_KEX2_1	375	377	PF00082	0.691
CLV_PCSK_KEX2_1	51	53	PF00082	0.560
CLV_PCSK_SKI1_1	323	327	PF00082	0.306
CLV_PCSK_SKI1_1	482	486	PF00082	0.633
CLV_PCSK_SKI1_1	532	536	PF00082	0.346
DEG_APCC_DBOX_1	225	233	PF00400	0.403
DEG_APCC_DBOX_1	299	307	PF00400	0.687
DEG_SCF_FBW7_1	281	288	PF00400	0.633
DEG_SPOP_SBC_1	175	179	PF00917	0.528
DEG_SPOP_SBC_1	386	390	PF00917	0.584
DEG_SPOP_SBC_1	461	465	PF00917	0.466
DOC_CDC14_PxL_1	157	165	PF14671	0.416
DOC_CYCLIN_yCln2_LP_2	232	238	PF00134	0.428
DOC_MAPK_DCC_7	226	234	PF00069	0.364
DOC_MAPK_HePTP_8	316	328	PF00069	0.515
DOC_MAPK_MEF2A_6	319	328	PF00069	0.545
DOC_PP1_RVXF_1	136	142	PF00149	0.382
DOC_PP1_RVXF_1	317	323	PF00149	0.589
DOC_PP1_RVXF_1	480	486	PF00149	0.429
DOC_PP1_RVXF_1	92	98	PF00149	0.579
DOC_PP2B_LxvP_1	133	136	PF13499	0.460
DOC_PP2B_LxvP_1	232	235	PF13499	0.385
DOC_PP4_FxxP_1	523	526	PF00568	0.500
DOC_PP4_FxxP_1	64	67	PF00568	0.703
DOC_SPAK_OSR1_1	115	119	PF12202	0.512
DOC_USP7_MATH_1	175	179	PF00917	0.518
DOC_USP7_MATH_1	277	281	PF00917	0.683
DOC_USP7_MATH_1	386	390	PF00917	0.532
DOC_USP7_MATH_1	437	441	PF00917	0.345
DOC_USP7_MATH_1	461	465	PF00917	0.449
DOC_USP7_MATH_1	90	94	PF00917	0.610
DOC_WW_Pin1_4	164	169	PF00397	0.462
DOC_WW_Pin1_4	281	286	PF00397	0.748
DOC_WW_Pin1_4	37	42	PF00397	0.651
DOC_WW_Pin1_4	59	64	PF00397	0.709
LIG_14-3-3_CanoR_1	115	121	PF00244	0.411
LIG_14-3-3_CanoR_1	124	128	PF00244	0.425
LIG_14-3-3_CanoR_1	138	142	PF00244	0.348
LIG_14-3-3_CanoR_1	206	216	PF00244	0.397
LIG_14-3-3_CanoR_1	81	89	PF00244	0.694
LIG_14-3-3_CanoR_1	98	106	PF00244	0.475
LIG_Actin_WH2_2	8	25	PF00022	0.450
LIG_Actin_WH2_2	99	117	PF00022	0.512
LIG_APCC_ABBA_1	32	37	PF00400	0.646
LIG_BIR_II_1	1	5	PF00653	0.424
LIG_BRCT_BRCA1_1	289	293	PF00533	0.590
LIG_BRCT_BRCA1_1	336	340	PF00533	0.459
LIG_BRCT_BRCA1_1	98	102	PF00533	0.546
LIG_deltaCOP1_diTrp_1	57	64	PF00928	0.758
LIG_FHA_1	103	109	PF00498	0.405
LIG_FHA_1	16	22	PF00498	0.459
LIG_FHA_1	428	434	PF00498	0.418
LIG_FHA_1	449	455	PF00498	0.408
LIG_FHA_2	235	241	PF00498	0.429
LIG_FHA_2	450	456	PF00498	0.430
LIG_FHA_2	520	526	PF00498	0.618
LIG_Integrin_isoDGR_2	256	258	PF01839	0.352
LIG_Integrin_RGD_1	359	361	PF01839	0.693
LIG_LIR_Apic_2	227	231	PF02991	0.374
LIG_LIR_Apic_2	404	409	PF02991	0.374
LIG_LIR_Apic_2	522	526	PF02991	0.500
LIG_LIR_Apic_2	62	67	PF02991	0.625
LIG_LIR_Gen_1	110	118	PF02991	0.411
LIG_LIR_Gen_1	148	158	PF02991	0.400
LIG_LIR_Gen_1	18	26	PF02991	0.404
LIG_LIR_Gen_1	250	259	PF02991	0.428
LIG_LIR_Gen_1	430	438	PF02991	0.376
LIG_LIR_Gen_1	465	475	PF02991	0.491
LIG_LIR_Gen_1	525	535	PF02991	0.618
LIG_LIR_LC3C_4	240	245	PF02991	0.450
LIG_LIR_Nem_3	110	114	PF02991	0.411
LIG_LIR_Nem_3	148	153	PF02991	0.418
LIG_LIR_Nem_3	159	163	PF02991	0.345
LIG_LIR_Nem_3	167	173	PF02991	0.358
LIG_LIR_Nem_3	18	22	PF02991	0.404
LIG_LIR_Nem_3	200	205	PF02991	0.402
LIG_LIR_Nem_3	250	255	PF02991	0.411
LIG_LIR_Nem_3	351	355	PF02991	0.348
LIG_LIR_Nem_3	428	432	PF02991	0.336
LIG_LIR_Nem_3	464	470	PF02991	0.506
LIG_LIR_Nem_3	499	503	PF02991	0.411
LIG_LIR_Nem_3	525	530	PF02991	0.556
LIG_LIR_Nem_3	59	64	PF02991	0.674
LIG_LYPXL_yS_3	160	163	PF13949	0.453
LIG_MYND_1	171	175	PF01753	0.554
LIG_MYND_1	63	67	PF01753	0.699
LIG_NRBOX	213	219	PF00104	0.446
LIG_PCNA_PIPBox_1	248	257	PF02747	0.471
LIG_Pex14_1	127	131	PF04695	0.481
LIG_Pex14_2	293	297	PF04695	0.591
LIG_SH2_CRK	111	115	PF00017	0.450
LIG_SH2_CRK	406	410	PF00017	0.346
LIG_SH2_CRK	527	531	PF00017	0.618
LIG_SH2_PTP2	432	435	PF00017	0.346
LIG_SH2_SRC	329	332	PF00017	0.224
LIG_SH2_SRC	432	435	PF00017	0.418
LIG_SH2_STAP1	287	291	PF00017	0.639
LIG_SH2_STAP1	519	523	PF00017	0.618
LIG_SH2_STAP1	72	76	PF00017	0.595
LIG_SH2_STAT3	72	75	PF00017	0.717
LIG_SH2_STAT5	223	226	PF00017	0.320
LIG_SH2_STAT5	24	27	PF00017	0.433
LIG_SH2_STAT5	252	255	PF00017	0.411
LIG_SH2_STAT5	287	290	PF00017	0.637
LIG_SH2_STAT5	33	36	PF00017	0.615
LIG_SH2_STAT5	353	356	PF00017	0.398
LIG_SH2_STAT5	432	435	PF00017	0.346
LIG_SH2_STAT5	503	506	PF00017	0.411
LIG_SH2_STAT5	527	530	PF00017	0.618
LIG_SH3_3	115	121	PF00018	0.407
LIG_SH3_3	165	171	PF00018	0.484
LIG_SUMO_SIM_par_1	104	110	PF11976	0.512
LIG_SUMO_SIM_par_1	240	246	PF11976	0.410
LIG_TRAF2_1	453	456	PF00917	0.395
LIG_TRAF2_1	67	70	PF00917	0.695
LIG_TYR_ITIM	158	163	PF00017	0.541
LIG_TYR_ITSM	428	435	PF00017	0.512
MOD_CK1_1	137	143	PF00069	0.457
MOD_CK1_1	169	175	PF00069	0.685
MOD_CK1_1	176	182	PF00069	0.619
MOD_CK1_1	190	196	PF00069	0.508
MOD_CK1_1	305	311	PF00069	0.590
MOD_CK1_1	440	446	PF00069	0.613
MOD_CK1_1	449	455	PF00069	0.443
MOD_CK1_1	463	469	PF00069	0.410
MOD_CK2_1	386	392	PF00069	0.664
MOD_CK2_1	449	455	PF00069	0.522
MOD_CMANNOS	58	61	PF00535	0.686
MOD_Cter_Amidation	373	376	PF01082	0.761
MOD_GlcNHglycan	1	4	PF01048	0.530
MOD_GlcNHglycan	178	181	PF01048	0.725
MOD_GlcNHglycan	193	196	PF01048	0.391
MOD_GlcNHglycan	263	266	PF01048	0.445
MOD_GlcNHglycan	27	30	PF01048	0.572
MOD_GlcNHglycan	309	312	PF01048	0.601
MOD_GlcNHglycan	336	339	PF01048	0.512
MOD_GlcNHglycan	439	442	PF01048	0.579
MOD_GlcNHglycan	83	86	PF01048	0.650
MOD_GlcNHglycan	99	102	PF01048	0.249
MOD_GSK3_1	169	176	PF00069	0.646
MOD_GSK3_1	187	194	PF00069	0.657
MOD_GSK3_1	230	237	PF00069	0.487
MOD_GSK3_1	243	250	PF00069	0.382
MOD_GSK3_1	267	274	PF00069	0.585
MOD_GSK3_1	277	284	PF00069	0.727
MOD_GSK3_1	302	309	PF00069	0.603
MOD_GSK3_1	363	370	PF00069	0.674
MOD_GSK3_1	387	394	PF00069	0.729
MOD_GSK3_1	436	443	PF00069	0.387
MOD_GSK3_1	462	469	PF00069	0.654
MOD_NEK2_1	102	107	PF00069	0.372
MOD_NEK2_1	116	121	PF00069	0.508
MOD_NEK2_1	131	136	PF00069	0.347
MOD_NEK2_1	143	148	PF00069	0.300
MOD_NEK2_1	15	20	PF00069	0.465
MOD_NEK2_1	243	248	PF00069	0.359
MOD_NEK2_1	306	311	PF00069	0.698
MOD_NEK2_1	334	339	PF00069	0.482
MOD_NEK2_1	385	390	PF00069	0.683
MOD_NEK2_1	448	453	PF00069	0.516
MOD_NEK2_1	462	467	PF00069	0.544
MOD_NEK2_2	247	252	PF00069	0.411
MOD_PIKK_1	143	149	PF00454	0.222
MOD_PIKK_1	207	213	PF00454	0.480
MOD_PIKK_1	65	71	PF00454	0.604
MOD_PKA_2	123	129	PF00069	0.559
MOD_PKA_2	137	143	PF00069	0.384
MOD_PKA_2	399	405	PF00069	0.416
MOD_PKA_2	80	86	PF00069	0.619
MOD_PKA_2	97	103	PF00069	0.336
MOD_Plk_4	137	143	PF00069	0.404
MOD_Plk_4	145	151	PF00069	0.385
MOD_Plk_4	15	21	PF00069	0.415
MOD_Plk_4	169	175	PF00069	0.690
MOD_Plk_4	197	203	PF00069	0.491
MOD_Plk_4	243	249	PF00069	0.378
MOD_Plk_4	463	469	PF00069	0.603
MOD_ProDKin_1	164	170	PF00069	0.588
MOD_ProDKin_1	281	287	PF00069	0.686
MOD_ProDKin_1	37	43	PF00069	0.563
MOD_ProDKin_1	59	65	PF00069	0.641
TRG_DiLeu_BaEn_1	159	164	PF01217	0.523
TRG_DiLeu_BaLyEn_6	320	325	PF01217	0.403
TRG_ENDOCYTIC_2	111	114	PF00928	0.512
TRG_ENDOCYTIC_2	154	157	PF00928	0.448
TRG_ENDOCYTIC_2	160	163	PF00928	0.461
TRG_ENDOCYTIC_2	252	255	PF00928	0.411
TRG_ENDOCYTIC_2	352	355	PF00928	0.437
TRG_ENDOCYTIC_2	432	435	PF00928	0.411
TRG_ENDOCYTIC_2	527	530	PF00928	0.411
TRG_ER_diArg_1	355	358	PF00400	0.511
TRG_ER_diArg_1	50	52	PF00400	0.714
TRG_NES_CRM1_1	147	159	PF08389	0.237
TRG_Pf-PMV_PEXEL_1	23	27	PF00026	0.450

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P837	Leptomonas seymouri	46%	100%
A4H965	Leishmania braziliensis	72%	100%
A4HXI7	Leishmania infantum	99%	100%
E9AR82	Leishmania mexicana (strain MHOM/GT/2001/U1103)	86%	100%
Q4QE81	Leishmania major	91%	100%