Amastin_surface_glycoprotein_putative/Pfam:PF0734 4

Quick info Annotations Localization Phosphorylation Expansion Sequence features Structure Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, unknown function

Species:

Leishmania donovani

UniProt:

A0A3S7WSV8_LEIDO

TriTrypDb:

LdBPK_140500.1 , LdCL_140010300 , LDHU3_14.0660

Length:

274

Annotations

LeishMANIAdb annotations

Structurally similar to the amastin family proteins, but with a rather divergent sequence. Also seems to be related to mammalian clarin proteins.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	yes	yes: 6
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 4

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	10
GO:0110165	cellular anatomical entity	1	10

Phosphorylation

Promastigote: 17

Expansion

Sequence features

A0A3S7WSV8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7WSV8

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	112	114	PF00675	0.684
CLV_NRD_NRD_1	214	216	PF00675	0.838
CLV_NRD_NRD_1	22	24	PF00675	0.652
CLV_NRD_NRD_1	29	31	PF00675	0.611
CLV_PCSK_KEX2_1	22	24	PF00082	0.652
CLV_PCSK_KEX2_1	29	31	PF00082	0.611
CLV_PCSK_SKI1_1	37	41	PF00082	0.596
DEG_Nend_UBRbox_1	1	4	PF02207	0.849
DOC_MAPK_gen_1	45	52	PF00069	0.636
DOC_MAPK_MEF2A_6	45	52	PF00069	0.636
DOC_PP2B_LxvP_1	40	43	PF13499	0.724
DOC_USP7_UBL2_3	216	220	PF12436	0.527
LIG_14-3-3_CanoR_1	22	28	PF00244	0.835
LIG_14-3-3_CanoR_1	256	261	PF00244	0.810
LIG_BRCT_BRCA1_1	101	105	PF00533	0.498
LIG_BRCT_BRCA1_1	225	229	PF00533	0.556
LIG_FHA_1	141	147	PF00498	0.457
LIG_FHA_1	165	171	PF00498	0.554
LIG_FHA_1	174	180	PF00498	0.628
LIG_FHA_1	53	59	PF00498	0.505
LIG_FHA_1	76	82	PF00498	0.467
LIG_LIR_Gen_1	139	150	PF02991	0.472
LIG_LIR_Gen_1	156	166	PF02991	0.531
LIG_LIR_Gen_1	174	181	PF02991	0.638
LIG_LIR_Gen_1	185	195	PF02991	0.471
LIG_LIR_Gen_1	221	230	PF02991	0.497
LIG_LIR_Gen_1	244	253	PF02991	0.463
LIG_LIR_Gen_1	60	69	PF02991	0.498
LIG_LIR_Nem_3	156	161	PF02991	0.508
LIG_LIR_Nem_3	174	178	PF02991	0.638
LIG_LIR_Nem_3	185	190	PF02991	0.441
LIG_LIR_Nem_3	221	225	PF02991	0.488
LIG_LIR_Nem_3	226	232	PF02991	0.453
LIG_LIR_Nem_3	244	248	PF02991	0.431
LIG_LIR_Nem_3	60	64	PF02991	0.498
LIG_Pex14_2	225	229	PF04695	0.503
LIG_Pex14_2	241	245	PF04695	0.519
LIG_PTB_Apo_2	130	137	PF02174	0.448
LIG_Rb_LxCxE_1	38	60	PF01857	0.639
LIG_SH2_CRK	158	162	PF00017	0.519
LIG_SH2_STAP1	142	146	PF00017	0.510
LIG_SH2_STAP1	201	205	PF00017	0.534
LIG_SH2_STAP1	249	253	PF00017	0.648
LIG_SH2_STAT5	126	129	PF00017	0.513
LIG_SH2_STAT5	142	145	PF00017	0.490
LIG_SH2_STAT5	222	225	PF00017	0.485
LIG_SH2_STAT5	249	252	PF00017	0.570
LIG_SH2_STAT5	41	44	PF00017	0.737
LIG_Sin3_3	61	68	PF02671	0.498
LIG_SUMO_SIM_anti_2	60	66	PF11976	0.498
LIG_SUMO_SIM_par_1	187	193	PF11976	0.605
LIG_WRC_WIRS_1	58	63	PF05994	0.498
MOD_CK1_1	138	144	PF00069	0.473
MOD_CK1_1	156	162	PF00069	0.400
MOD_CK1_1	17	23	PF00069	0.857
MOD_CK1_1	204	210	PF00069	0.609
MOD_CK2_1	94	100	PF00069	0.582
MOD_Cter_Amidation	213	216	PF01082	0.841
MOD_GlcNHglycan	90	93	PF01048	0.721
MOD_GSK3_1	10	17	PF00069	0.825
MOD_GSK3_1	115	122	PF00069	0.506
MOD_GSK3_1	136	143	PF00069	0.503
MOD_GSK3_1	18	25	PF00069	0.792
MOD_GSK3_1	190	197	PF00069	0.585
MOD_GSK3_1	204	211	PF00069	0.460
MOD_GSK3_1	243	250	PF00069	0.618
MOD_N-GLC_1	208	213	PF02516	0.828
MOD_NEK2_1	105	110	PF00069	0.395
MOD_NEK2_1	18	23	PF00069	0.924
MOD_NEK2_1	208	213	PF00069	0.572
MOD_NEK2_1	225	230	PF00069	0.371
MOD_NEK2_1	231	236	PF00069	0.503
MOD_NEK2_1	241	246	PF00069	0.519
MOD_NEK2_1	52	57	PF00069	0.542
MOD_NEK2_1	94	99	PF00069	0.530
MOD_NEK2_2	116	121	PF00069	0.599
MOD_PKA_1	22	28	PF00069	0.835
MOD_PKA_1	29	35	PF00069	0.804
MOD_PKA_2	18	24	PF00069	0.853
MOD_PKA_2	29	35	PF00069	0.804
MOD_Plk_1	13	19	PF00069	0.850
MOD_Plk_1	173	179	PF00069	0.653
MOD_Plk_1	208	214	PF00069	0.632
MOD_Plk_4	105	111	PF00069	0.475
MOD_Plk_4	140	146	PF00069	0.443
MOD_Plk_4	156	162	PF00069	0.631
MOD_Plk_4	208	214	PF00069	0.589
MOD_Plk_4	256	262	PF00069	0.794
MOD_SUMO_rev_2	259	267	PF00179	0.757
TRG_ENDOCYTIC_2	142	145	PF00928	0.553
TRG_ENDOCYTIC_2	158	161	PF00928	0.476
TRG_ENDOCYTIC_2	222	225	PF00928	0.485
TRG_ENDOCYTIC_2	249	252	PF00928	0.570
TRG_ER_diArg_1	22	24	PF00400	0.924
TRG_NES_CRM1_1	86	100	PF08389	0.580
TRG_NLS_MonoExtN_4	29	34	PF00514	0.842
TRG_Pf-PMV_PEXEL_1	269	273	PF00026	0.511

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P2B8	Leptomonas seymouri	71%	99%
A0A3S5H6R5	Leishmania donovani	98%	100%
A4H7K8	Leishmania braziliensis	77%	100%
A4H7K9	Leishmania braziliensis	78%	100%
A4HVZ0	Leishmania infantum	100%	100%
A4HVZ1	Leishmania infantum	97%	100%
E9APP2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	85%	100%
E9APP3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	100%
Q4QFT6	Leishmania major	92%	100%
Q4QFT7	Leishmania major	93%	100%