tRNA-methyl transferase, putative

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA-methyl transferase, putative

Species:

Leishmania donovani

UniProt:

A0A3S7WPQ8_LEIDO

TriTrypDb:

LdBPK_070280.1 , LdCL_070006300 , LDHU3_07.0180

Length:

613

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

A0A3S7WPQ8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7WPQ8

Function

Biological processes
Term	Name	Level	Count
GO:0002097	tRNA wobble base modification	7	1
GO:0002098	tRNA wobble uridine modification	8	1
GO:0002143	tRNA wobble position uridine thiolation	8	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006396	RNA processing	6	1
GO:0006399	tRNA metabolic process	7	1
GO:0006400	tRNA modification	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008033	tRNA processing	8	1
GO:0008152	metabolic process	1	1
GO:0009451	RNA modification	5	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0034227	tRNA thio-modification	7	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	10
GO:0004808	tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase activity	6	8
GO:0008168	methyltransferase activity	4	8
GO:0008173	RNA methyltransferase activity	4	8
GO:0008175	tRNA methyltransferase activity	5	8
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	8
GO:0016740	transferase activity	2	10
GO:0016741	transferase activity, transferring one-carbon groups	3	8
GO:0140098	catalytic activity, acting on RNA	3	8
GO:0140101	catalytic activity, acting on a tRNA	4	8
GO:0140640	catalytic activity, acting on a nucleic acid	2	8

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	575	579	PF00656	0.769
CLV_NRD_NRD_1	187	189	PF00675	0.361
CLV_NRD_NRD_1	221	223	PF00675	0.305
CLV_NRD_NRD_1	300	302	PF00675	0.526
CLV_NRD_NRD_1	415	417	PF00675	0.552
CLV_NRD_NRD_1	536	538	PF00675	0.305
CLV_PCSK_FUR_1	534	538	PF00082	0.305
CLV_PCSK_KEX2_1	187	189	PF00082	0.361
CLV_PCSK_KEX2_1	220	222	PF00082	0.305
CLV_PCSK_KEX2_1	415	417	PF00082	0.583
CLV_PCSK_KEX2_1	536	538	PF00082	0.305
CLV_PCSK_PC1ET2_1	220	222	PF00082	0.305
CLV_PCSK_SKI1_1	222	226	PF00082	0.340
CLV_PCSK_SKI1_1	349	353	PF00082	0.505
CLV_PCSK_SKI1_1	547	551	PF00082	0.273
CLV_PCSK_SKI1_1	74	78	PF00082	0.604
DOC_ANK_TNKS_1	414	421	PF00023	0.553
DOC_CKS1_1	94	99	PF01111	0.561
DOC_CYCLIN_RxL_1	125	136	PF00134	0.509
DOC_CYCLIN_yCln2_LP_2	399	405	PF00134	0.404
DOC_CYCLIN_yCln2_LP_2	481	487	PF00134	0.536
DOC_MAPK_gen_1	220	227	PF00069	0.505
DOC_MAPK_gen_1	301	310	PF00069	0.486
DOC_MAPK_gen_1	534	542	PF00069	0.537
DOC_MAPK_MEF2A_6	220	227	PF00069	0.505
DOC_MAPK_MEF2A_6	7	16	PF00069	0.541
DOC_MAPK_NFAT4_5	220	228	PF00069	0.408
DOC_PP1_RVXF_1	174	180	PF00149	0.537
DOC_PP1_RVXF_1	537	543	PF00149	0.561
DOC_PP2B_LxvP_1	337	340	PF13499	0.583
DOC_PP2B_LxvP_1	361	364	PF13499	0.559
DOC_PP2B_LxvP_1	481	484	PF13499	0.544
DOC_PP4_FxxP_1	310	313	PF00568	0.615
DOC_PP4_FxxP_1	45	48	PF00568	0.585
DOC_PP4_FxxP_1	542	545	PF00568	0.451
DOC_USP7_MATH_1	272	276	PF00917	0.537
DOC_USP7_MATH_1	284	288	PF00917	0.702
DOC_USP7_MATH_1	344	348	PF00917	0.603
DOC_USP7_MATH_1	436	440	PF00917	0.555
DOC_WW_Pin1_4	135	140	PF00397	0.561
DOC_WW_Pin1_4	547	552	PF00397	0.494
DOC_WW_Pin1_4	579	584	PF00397	0.680
DOC_WW_Pin1_4	594	599	PF00397	0.594
DOC_WW_Pin1_4	93	98	PF00397	0.557
LIG_14-3-3_CanoR_1	228	236	PF00244	0.477
LIG_14-3-3_CanoR_1	273	277	PF00244	0.548
LIG_14-3-3_CanoR_1	301	311	PF00244	0.492
LIG_14-3-3_CanoR_1	31	39	PF00244	0.427
LIG_14-3-3_CanoR_1	415	419	PF00244	0.555
LIG_APCC_ABBAyCdc20_2	537	543	PF00400	0.533
LIG_BIR_II_1	1	5	PF00653	0.630
LIG_BIR_III_2	578	582	PF00653	0.759
LIG_BIR_III_2	89	93	PF00653	0.516
LIG_BRCT_BRCA1_1	325	329	PF00533	0.632
LIG_Clathr_ClatBox_1	561	565	PF01394	0.378
LIG_CSL_BTD_1	479	482	PF09270	0.478
LIG_EVH1_1	337	341	PF00568	0.550
LIG_FHA_1	164	170	PF00498	0.470
LIG_FHA_1	181	187	PF00498	0.457
LIG_FHA_1	204	210	PF00498	0.471
LIG_FHA_1	23	29	PF00498	0.399
LIG_FHA_1	238	244	PF00498	0.452
LIG_FHA_1	280	286	PF00498	0.740
LIG_FHA_1	34	40	PF00498	0.289
LIG_FHA_1	346	352	PF00498	0.468
LIG_FHA_1	605	611	PF00498	0.563
LIG_FHA_2	33	39	PF00498	0.429
LIG_FHA_2	355	361	PF00498	0.486
LIG_FHA_2	75	81	PF00498	0.591
LIG_Integrin_RGD_1	576	578	PF01839	0.618
LIG_LIR_Apic_2	206	210	PF02991	0.505
LIG_LIR_Apic_2	335	339	PF02991	0.519
LIG_LIR_Apic_2	476	482	PF02991	0.490
LIG_LIR_Nem_3	326	332	PF02991	0.580
LIG_LIR_Nem_3	395	399	PF02991	0.551
LIG_LYPXL_SIV_4	426	434	PF13949	0.443
LIG_LYPXL_yS_3	396	399	PF13949	0.482
LIG_MYND_1	397	401	PF01753	0.461
LIG_MYND_3	559	563	PF01753	0.457
LIG_PDZ_Class_2	608	613	PF00595	0.463
LIG_Pex14_2	99	103	PF04695	0.457
LIG_PROFILIN_1	338	344	PF00235	0.481
LIG_REV1ctd_RIR_1	96	105	PF16727	0.505
LIG_SH2_CRK	558	562	PF00017	0.443
LIG_SH2_NCK_1	120	124	PF00017	0.488
LIG_SH2_NCK_1	88	92	PF00017	0.498
LIG_SH2_SRC	120	123	PF00017	0.537
LIG_SH2_SRC	336	339	PF00017	0.580
LIG_SH2_STAP1	203	207	PF00017	0.457
LIG_SH2_STAP1	403	407	PF00017	0.385
LIG_SH2_STAP1	468	472	PF00017	0.501
LIG_SH2_STAT3	149	152	PF00017	0.537
LIG_SH2_STAT3	468	471	PF00017	0.527
LIG_SH2_STAT5	149	152	PF00017	0.455
LIG_SH2_STAT5	336	339	PF00017	0.541
LIG_SH2_STAT5	428	431	PF00017	0.367
LIG_SH2_STAT5	95	98	PF00017	0.445
LIG_SH3_1	336	342	PF00018	0.634
LIG_SH3_2	51	56	PF14604	0.359
LIG_SH3_3	308	314	PF00018	0.584
LIG_SH3_3	335	341	PF00018	0.622
LIG_SH3_3	372	378	PF00018	0.500
LIG_SH3_3	430	436	PF00018	0.541
LIG_SH3_3	45	51	PF00018	0.590
LIG_SH3_3	578	584	PF00018	0.743
LIG_SH3_3	592	598	PF00018	0.327
LIG_SUMO_SIM_anti_2	607	612	PF11976	0.605
LIG_SUMO_SIM_par_1	560	565	PF11976	0.516
LIG_SUMO_SIM_par_1	606	612	PF11976	0.502
LIG_TRAF2_1	357	360	PF00917	0.561
LIG_TRAF2_1	523	526	PF00917	0.561
LIG_TYR_ITIM	556	561	PF00017	0.335
LIG_UBA3_1	561	569	PF00899	0.531
LIG_WW_2	343	346	PF00397	0.451
MOD_CK1_1	191	197	PF00069	0.455
MOD_CK1_1	2	8	PF00069	0.604
MOD_CK1_1	22	28	PF00069	0.146
MOD_CK1_1	279	285	PF00069	0.613
MOD_CK1_1	315	321	PF00069	0.663
MOD_CK1_1	324	330	PF00069	0.637
MOD_CK1_1	347	353	PF00069	0.542
MOD_CK1_1	440	446	PF00069	0.689
MOD_CK1_1	470	476	PF00069	0.503
MOD_CK1_1	597	603	PF00069	0.625
MOD_CK2_1	285	291	PF00069	0.708
MOD_CK2_1	354	360	PF00069	0.485
MOD_CK2_1	579	585	PF00069	0.651
MOD_CK2_1	74	80	PF00069	0.586
MOD_DYRK1A_RPxSP_1	547	551	PF00069	0.374
MOD_GlcNHglycan	109	112	PF01048	0.429
MOD_GlcNHglycan	16	19	PF01048	0.365
MOD_GlcNHglycan	190	193	PF01048	0.352
MOD_GlcNHglycan	21	24	PF01048	0.332
MOD_GlcNHglycan	230	233	PF01048	0.323
MOD_GlcNHglycan	248	251	PF01048	0.330
MOD_GlcNHglycan	278	281	PF01048	0.692
MOD_GlcNHglycan	314	317	PF01048	0.625
MOD_GlcNHglycan	524	530	PF01048	0.455
MOD_GlcNHglycan	591	594	PF01048	0.409
MOD_GSK3_1	193	200	PF00069	0.381
MOD_GSK3_1	237	244	PF00069	0.330
MOD_GSK3_1	246	253	PF00069	0.330
MOD_GSK3_1	272	279	PF00069	0.539
MOD_GSK3_1	297	304	PF00069	0.457
MOD_GSK3_1	320	327	PF00069	0.692
MOD_GSK3_1	436	443	PF00069	0.585
MOD_NEK2_1	172	177	PF00069	0.420
MOD_NEK2_1	186	191	PF00069	0.245
MOD_NEK2_1	193	198	PF00069	0.331
MOD_NEK2_1	276	281	PF00069	0.615
MOD_NEK2_1	467	472	PF00069	0.401
MOD_NEK2_1	606	611	PF00069	0.615
MOD_OFUCOSY	319	325	PF10250	0.588
MOD_PIKK_1	467	473	PF00454	0.508
MOD_PIKK_1	5	11	PF00454	0.537
MOD_PKA_1	301	307	PF00069	0.476
MOD_PKA_2	186	192	PF00069	0.455
MOD_PKA_2	272	278	PF00069	0.468
MOD_PKA_2	414	420	PF00069	0.555
MOD_Plk_1	237	243	PF00069	0.310
MOD_Plk_4	193	199	PF00069	0.365
MOD_Plk_4	237	243	PF00069	0.318
MOD_Plk_4	606	612	PF00069	0.593
MOD_ProDKin_1	135	141	PF00069	0.455
MOD_ProDKin_1	547	553	PF00069	0.362
MOD_ProDKin_1	579	585	PF00069	0.669
MOD_ProDKin_1	594	600	PF00069	0.600
MOD_ProDKin_1	93	99	PF00069	0.450
MOD_SUMO_rev_2	439	448	PF00179	0.671
TRG_DiLeu_BaLyEn_6	448	453	PF01217	0.451
TRG_DiLeu_BaLyEn_6	557	562	PF01217	0.432
TRG_ENDOCYTIC_2	396	399	PF00928	0.536
TRG_ENDOCYTIC_2	558	561	PF00928	0.341
TRG_ENDOCYTIC_2	95	98	PF00928	0.275
TRG_ER_diArg_1	186	188	PF00400	0.455
TRG_ER_diArg_1	221	223	PF00400	0.377
TRG_ER_diArg_1	410	413	PF00400	0.498
TRG_ER_diArg_1	533	536	PF00400	0.420
TRG_ER_diArg_1	537	540	PF00400	0.425
TRG_Pf-PMV_PEXEL_1	234	238	PF00026	0.422
TRG_Pf-PMV_PEXEL_1	451	455	PF00026	0.488

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I0V4	Leptomonas seymouri	49%	100%
A0A1X0NK59	Trypanosomatidae	37%	100%
A0A422NTD5	Trypanosoma rangeli	42%	100%
A4H4V6	Leishmania braziliensis	79%	98%
A4HT56	Leishmania infantum	100%	100%
C9ZUV8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	100%
E9AL23	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
Q4QIT1	Leishmania major	93%	100%
Q503J2	Danio rerio	25%	100%
Q5RB73	Pongo abelii	26%	100%
Q5ZKW0	Gallus gallus	27%	100%
V5BDF2	Trypanosoma cruzi	39%	100%