Ribonuclease H1, putative

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ribonuclease H1, putative

Species:

Leishmania donovani

UniProt:

A0A3S7WPF2_LEIDO

TriTrypDb:

LdBPK_060290.1 , LdCL_060008000 , LDHU3_06.0360

Length:

250

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

A0A3S7WPF2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S7WPF2

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006401	RNA catabolic process	5	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0009057	macromolecule catabolic process	4	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0019439	aromatic compound catabolic process	4	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034655	nucleobase-containing compound catabolic process	4	1
GO:0043137	DNA replication, removal of RNA primer	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0044265	obsolete cellular macromolecule catabolic process	4	1
GO:0044270	cellular nitrogen compound catabolic process	4	1
GO:0046483	heterocycle metabolic process	3	1
GO:0046700	heterocycle catabolic process	4	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:0090305	nucleic acid phosphodiester bond hydrolysis	5	1
GO:0090501	RNA phosphodiester bond hydrolysis	6	1
GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic	7	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901361	organic cyclic compound catabolic process	4	1
GO:1901575	organic substance catabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000287	magnesium ion binding	5	12
GO:0003676	nucleic acid binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004518	nuclease activity	4	12
GO:0004519	endonuclease activity	5	12
GO:0004521	RNA endonuclease activity	5	12
GO:0004523	RNA-DNA hybrid ribonuclease activity	7	12
GO:0004540	RNA nuclease activity	4	12
GO:0005488	binding	1	12
GO:0016787	hydrolase activity	2	12
GO:0016788	hydrolase activity, acting on ester bonds	3	12
GO:0016891	RNA endonuclease activity, producing 5'-phosphomonoesters	6	12
GO:0016893	endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	6	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0097159	organic cyclic compound binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	235	239	PF00656	0.521
CLV_PCSK_SKI1_1	140	144	PF00082	0.425
CLV_Separin_Metazoa	203	207	PF03568	0.549
DEG_SPOP_SBC_1	19	23	PF00917	0.686
DOC_ANK_TNKS_1	188	195	PF00023	0.535
DOC_CYCLIN_yCln2_LP_2	118	124	PF00134	0.258
DOC_CYCLIN_yCln2_LP_2	201	207	PF00134	0.551
DOC_MAPK_MEF2A_6	120	127	PF00069	0.300
DOC_PP4_FxxP_1	64	67	PF00568	0.218
DOC_USP7_MATH_1	8	12	PF00917	0.664
DOC_WW_Pin1_4	23	28	PF00397	0.615
DOC_WW_Pin1_4	6	11	PF00397	0.638
LIG_14-3-3_CanoR_1	173	178	PF00244	0.623
LIG_14-3-3_CanoR_1	206	213	PF00244	0.575
LIG_14-3-3_CanoR_1	62	67	PF00244	0.296
LIG_14-3-3_CterR_2	246	250	PF00244	0.561
LIG_APCC_ABBA_1	125	130	PF00400	0.238
LIG_BIR_II_1	1	5	PF00653	0.552
LIG_FHA_1	103	109	PF00498	0.440
LIG_FHA_1	117	123	PF00498	0.118
LIG_FHA_2	210	216	PF00498	0.611
LIG_KLC1_Yacidic_2	31	36	PF13176	0.313
LIG_LIR_Gen_1	172	179	PF02991	0.684
LIG_LIR_Nem_3	111	116	PF02991	0.251
LIG_LIR_Nem_3	162	167	PF02991	0.249
LIG_LIR_Nem_3	172	177	PF02991	0.486
LIG_SH2_CRK	174	178	PF00017	0.651
LIG_SH2_SRC	34	37	PF00017	0.238
LIG_SH2_STAP1	141	145	PF00017	0.365
LIG_SH2_STAT3	178	181	PF00017	0.648
LIG_SH2_STAT3	207	210	PF00017	0.593
LIG_SH2_STAT5	128	131	PF00017	0.261
LIG_SH2_STAT5	133	136	PF00017	0.216
LIG_SH2_STAT5	207	210	PF00017	0.740
LIG_SH2_STAT5	34	37	PF00017	0.238
LIG_SUMO_SIM_anti_2	217	224	PF11976	0.588
LIG_SUMO_SIM_anti_2	238	245	PF11976	0.546
LIG_TRAF2_2	157	162	PF00917	0.300
MOD_CK1_1	6	12	PF00069	0.613
MOD_CK2_1	150	156	PF00069	0.365
MOD_GlcNHglycan	109	113	PF01048	0.300
MOD_GlcNHglycan	5	8	PF01048	0.645
MOD_GSK3_1	17	24	PF00069	0.666
MOD_GSK3_1	205	212	PF00069	0.720
MOD_GSK3_1	37	44	PF00069	0.333
MOD_GSK3_1	6	13	PF00069	0.678
MOD_GSK3_1	68	75	PF00069	0.371
MOD_LATS_1	224	230	PF00433	0.561
MOD_N-GLC_1	116	121	PF02516	0.202
MOD_N-GLC_1	41	46	PF02516	0.337
MOD_N-GLC_1	62	67	PF02516	0.329
MOD_NEK2_1	1	6	PF00069	0.645
MOD_NEK2_1	102	107	PF00069	0.383
MOD_NEK2_1	150	155	PF00069	0.295
MOD_NEK2_1	18	23	PF00069	0.694
MOD_NEK2_1	70	75	PF00069	0.300
MOD_PIKK_1	70	76	PF00454	0.440
MOD_PKA_2	205	211	PF00069	0.707
MOD_PKB_1	171	179	PF00069	0.642
MOD_Plk_1	102	108	PF00069	0.230
MOD_Plk_4	173	179	PF00069	0.552
MOD_ProDKin_1	23	29	PF00069	0.615
MOD_ProDKin_1	6	12	PF00069	0.638
TRG_DiLeu_BaEn_1	103	108	PF01217	0.230
TRG_DiLeu_BaEn_1	218	223	PF01217	0.592
TRG_ENDOCYTIC_2	174	177	PF00928	0.594
TRG_ENDOCYTIC_2	55	58	PF00928	0.400
TRG_ER_diArg_1	168	171	PF00400	0.290

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PC83	Leptomonas seymouri	61%	72%
A0A0S4IX23	Bodo saltans	38%	82%
A0A1X0NJL6	Trypanosomatidae	32%	69%
A0A3R7MD61	Trypanosoma rangeli	38%	68%
A4H4J5	Leishmania braziliensis	83%	100%
A4HSS0	Leishmania infantum	99%	100%
C9ZTC3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	83%
E9AKQ7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
O60930	Homo sapiens	28%	87%
O70338	Mus musculus	27%	88%
Q4QJ50	Leishmania major	95%	100%
Q5BK46	Rattus norvegicus	27%	88%
Q9UST8	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	29%	95%
V5BEV1	Trypanosoma cruzi	37%	77%