General transcription factor IIH subunit 4

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

TFIIH basal transcription factor subunit, putative

Species:

Leishmania donovani

UniProt:

A0A3S5H816_LEIDO

TriTrypDb:

LdBPK_360860.1 , LdCL_360013600 , LDHU3_36.1130

Length:

557

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000439	transcription factor TFIIH core complex	4	12
GO:0005667	transcription regulator complex	2	12
GO:0032991	protein-containing complex	1	12
GO:0090575	RNA polymerase II transcription regulator complex	3	12
GO:0140513	nuclear protein-containing complex	2	12
GO:0005654	nucleoplasm	2	1
GO:0005675	transcription factor TFIIH holo complex	4	1
GO:0032806	carboxy-terminal domain protein kinase complex	3	1
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	1
GO:0110165	cellular anatomical entity	1	1
GO:1902494	catalytic complex	2	1
GO:1902554	serine/threonine protein kinase complex	6	1
GO:1902911	protein kinase complex	5	1
GO:1990234	transferase complex	3	1

Expansion

Sequence features

A0A3S5H816

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S5H816

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006259	DNA metabolic process	4	12
GO:0006281	DNA repair	5	12
GO:0006289	nucleotide-excision repair	6	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0006950	response to stress	2	12
GO:0006974	DNA damage response	4	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0033554	cellular response to stress	3	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0046483	heterocycle metabolic process	3	12
GO:0050896	response to stimulus	1	12
GO:0051716	cellular response to stimulus	2	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006355	regulation of DNA-templated transcription	6	1
GO:0006357	regulation of transcription by RNA polymerase II	7	1
GO:0006468	protein phosphorylation	5	1
GO:0006793	phosphorus metabolic process	3	1
GO:0006796	phosphate-containing compound metabolic process	4	1
GO:0009889	regulation of biosynthetic process	4	1
GO:0010468	regulation of gene expression	5	1
GO:0010556	regulation of macromolecule biosynthetic process	5	1
GO:0016310	phosphorylation	5	1
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	1
GO:0019222	regulation of metabolic process	3	1
GO:0019538	protein metabolic process	3	1
GO:0031323	regulation of cellular metabolic process	4	1
GO:0031326	regulation of cellular biosynthetic process	5	1
GO:0036211	protein modification process	4	1
GO:0043412	macromolecule modification	4	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0051171	regulation of nitrogen compound metabolic process	4	1
GO:0051252	regulation of RNA metabolic process	5	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0065007	biological regulation	1	1
GO:0070816	obsolete phosphorylation of RNA polymerase II C-terminal domain	6	1
GO:0080090	regulation of primary metabolic process	4	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1903506	regulation of nucleic acid-templated transcription	7	1
GO:2001141	regulation of RNA biosynthetic process	6	1

Molecular functions
Term	Name	Level	Count
GO:0001671	ATPase activator activity	3	12
GO:0098772	molecular function regulator activity	1	12
GO:0140677	molecular function activator activity	2	12
GO:0003676	nucleic acid binding	3	1
GO:0003677	DNA binding	4	1
GO:0003690	double-stranded DNA binding	5	1
GO:0005488	binding	1	1
GO:0097159	organic cyclic compound binding	2	1
GO:1901363	heterocyclic compound binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	472	476	PF00656	0.689
CLV_NRD_NRD_1	205	207	PF00675	0.359
CLV_NRD_NRD_1	309	311	PF00675	0.416
CLV_NRD_NRD_1	350	352	PF00675	0.344
CLV_NRD_NRD_1	406	408	PF00675	0.381
CLV_NRD_NRD_1	429	431	PF00675	0.667
CLV_NRD_NRD_1	461	463	PF00675	0.733
CLV_NRD_NRD_1	48	50	PF00675	0.472
CLV_NRD_NRD_1	498	500	PF00675	0.476
CLV_PCSK_KEX2_1	160	162	PF00082	0.552
CLV_PCSK_KEX2_1	350	352	PF00082	0.344
CLV_PCSK_KEX2_1	429	431	PF00082	0.633
CLV_PCSK_KEX2_1	460	462	PF00082	0.739
CLV_PCSK_KEX2_1	48	50	PF00082	0.465
CLV_PCSK_KEX2_1	498	500	PF00082	0.462
CLV_PCSK_PC1ET2_1	160	162	PF00082	0.552
CLV_PCSK_SKI1_1	118	122	PF00082	0.561
CLV_PCSK_SKI1_1	160	164	PF00082	0.444
CLV_PCSK_SKI1_1	186	190	PF00082	0.437
CLV_PCSK_SKI1_1	207	211	PF00082	0.385
CLV_PCSK_SKI1_1	235	239	PF00082	0.490
CLV_PCSK_SKI1_1	258	262	PF00082	0.396
CLV_PCSK_SKI1_1	408	412	PF00082	0.425
CLV_PCSK_SKI1_1	499	503	PF00082	0.498
CLV_PCSK_SKI1_1	518	522	PF00082	0.341
DEG_APCC_DBOX_1	117	125	PF00400	0.548
DEG_SPOP_SBC_1	2	6	PF00917	0.513
DOC_CKS1_1	196	201	PF01111	0.398
DOC_CYCLIN_RxL_1	113	122	PF00134	0.593
DOC_CYCLIN_RxL_1	232	240	PF00134	0.413
DOC_MAPK_FxFP_2	278	281	PF00069	0.417
DOC_PP1_RVXF_1	256	263	PF00149	0.449
DOC_PP1_RVXF_1	496	503	PF00149	0.482
DOC_PP2B_LxvP_1	480	483	PF13499	0.534
DOC_PP2B_LxvP_1	551	554	PF13499	0.486
DOC_PP2B_LxvP_1	8	11	PF13499	0.602
DOC_PP4_FxxP_1	132	135	PF00568	0.667
DOC_PP4_FxxP_1	278	281	PF00568	0.417
DOC_USP7_MATH_1	2	6	PF00917	0.592
DOC_USP7_MATH_1	423	427	PF00917	0.344
DOC_USP7_MATH_1	449	453	PF00917	0.676
DOC_WW_Pin1_4	137	142	PF00397	0.630
DOC_WW_Pin1_4	195	200	PF00397	0.395
DOC_WW_Pin1_4	207	212	PF00397	0.448
DOC_WW_Pin1_4	313	318	PF00397	0.337
DOC_WW_Pin1_4	326	331	PF00397	0.306
DOC_WW_Pin1_4	439	444	PF00397	0.758
DOC_WW_Pin1_4	455	460	PF00397	0.479
LIG_14-3-3_CanoR_1	102	106	PF00244	0.536
LIG_14-3-3_CanoR_1	118	128	PF00244	0.555
LIG_14-3-3_CanoR_1	154	163	PF00244	0.509
LIG_14-3-3_CanoR_1	295	303	PF00244	0.340
LIG_14-3-3_CanoR_1	407	416	PF00244	0.353
LIG_14-3-3_CanoR_1	417	422	PF00244	0.396
LIG_14-3-3_CanoR_1	510	520	PF00244	0.537
LIG_14-3-3_CanoR_1	70	79	PF00244	0.491
LIG_Actin_WH2_2	149	166	PF00022	0.488
LIG_BRCT_BRCA1_1	158	162	PF00533	0.543
LIG_BRCT_BRCA1_1	315	319	PF00533	0.352
LIG_BRCT_BRCA1_1	331	335	PF00533	0.344
LIG_BRCT_BRCA1_1	449	453	PF00533	0.740
LIG_BRCT_BRCA1_1	72	76	PF00533	0.536
LIG_CaM_IQ_9	41	57	PF13499	0.593
LIG_CtBP_PxDLS_1	330	334	PF00389	0.437
LIG_FHA_1	166	172	PF00498	0.507
LIG_FHA_1	173	179	PF00498	0.491
LIG_FHA_1	255	261	PF00498	0.413
LIG_FHA_1	352	358	PF00498	0.461
LIG_FHA_1	469	475	PF00498	0.722
LIG_FHA_1	477	483	PF00498	0.566
LIG_FHA_1	519	525	PF00498	0.552
LIG_FHA_2	138	144	PF00498	0.665
LIG_FHA_2	470	476	PF00498	0.736
LIG_FHA_2	85	91	PF00498	0.650
LIG_HCF-1_HBM_1	77	80	PF13415	0.349
LIG_LIR_Apic_2	106	112	PF02991	0.584
LIG_LIR_Gen_1	215	221	PF02991	0.463
LIG_LIR_Gen_1	537	548	PF02991	0.438
LIG_LIR_Gen_1	73	82	PF02991	0.545
LIG_LIR_LC3C_4	478	482	PF02991	0.585
LIG_LIR_Nem_3	215	219	PF02991	0.463
LIG_LIR_Nem_3	360	366	PF02991	0.317
LIG_LIR_Nem_3	537	543	PF02991	0.441
LIG_LIR_Nem_3	545	551	PF02991	0.408
LIG_LIR_Nem_3	73	79	PF02991	0.498
LIG_LYPXL_S_1	302	306	PF13949	0.344
LIG_LYPXL_yS_3	303	306	PF13949	0.344
LIG_NRBOX	260	266	PF00104	0.344
LIG_NRBOX	62	68	PF00104	0.526
LIG_PCNA_PIPBox_1	517	526	PF02747	0.566
LIG_PCNA_yPIPBox_3	181	189	PF02747	0.522
LIG_PCNA_yPIPBox_3	224	237	PF02747	0.475
LIG_PCNA_yPIPBox_3	510	524	PF02747	0.568
LIG_SH2_CRK	540	544	PF00017	0.462
LIG_SH2_SRC	37	40	PF00017	0.500
LIG_SH2_STAP1	368	372	PF00017	0.437
LIG_SH2_STAT5	276	279	PF00017	0.417
LIG_SH2_STAT5	368	371	PF00017	0.316
LIG_SH2_STAT5	523	526	PF00017	0.568
LIG_SH3_3	143	149	PF00018	0.571
LIG_SH3_3	193	199	PF00018	0.413
LIG_SH3_3	253	259	PF00018	0.437
LIG_SH3_3	311	317	PF00018	0.377
LIG_SUMO_SIM_anti_2	234	240	PF11976	0.395
LIG_SUMO_SIM_par_1	148	153	PF11976	0.580
LIG_SUMO_SIM_par_1	234	240	PF11976	0.385
LIG_SUMO_SIM_par_1	263	269	PF11976	0.475
LIG_SUMO_SIM_par_1	328	334	PF11976	0.437
LIG_TRFH_1	145	149	PF08558	0.612
LIG_TRFH_1	313	317	PF08558	0.340
LIG_TYR_ITIM	301	306	PF00017	0.385
LIG_TYR_ITIM	35	40	PF00017	0.456
LIG_UBA3_1	374	383	PF00899	0.262
LIG_WW_3	203	207	PF00397	0.359
MOD_CDC14_SPxK_1	458	461	PF00782	0.634
MOD_CDK_SPK_2	455	460	PF00069	0.635
MOD_CDK_SPxK_1	455	461	PF00069	0.636
MOD_CDK_SPxxK_3	455	462	PF00069	0.540
MOD_CK1_1	18	24	PF00069	0.404
MOD_CK1_1	251	257	PF00069	0.401
MOD_CK1_1	3	9	PF00069	0.636
MOD_CK1_1	344	350	PF00069	0.443
MOD_CK1_1	401	407	PF00069	0.390
MOD_CK1_1	442	448	PF00069	0.685
MOD_CK1_1	451	457	PF00069	0.679
MOD_CK1_1	470	476	PF00069	0.419
MOD_CK2_1	137	143	PF00069	0.689
MOD_CK2_1	68	74	PF00069	0.405
MOD_CK2_1	84	90	PF00069	0.560
MOD_Cter_Amidation	46	49	PF01082	0.492
MOD_GlcNHglycan	250	253	PF01048	0.375
MOD_GlcNHglycan	343	346	PF01048	0.423
MOD_GlcNHglycan	403	406	PF01048	0.350
MOD_GlcNHglycan	444	447	PF01048	0.661
MOD_GlcNHglycan	455	458	PF01048	0.647
MOD_GlcNHglycan	5	8	PF01048	0.573
MOD_GSK3_1	247	254	PF00069	0.463
MOD_GSK3_1	266	273	PF00069	0.475
MOD_GSK3_1	325	332	PF00069	0.353
MOD_GSK3_1	437	444	PF00069	0.691
MOD_GSK3_1	447	454	PF00069	0.695
MOD_GSK3_1	464	471	PF00069	0.477
MOD_GSK3_1	80	87	PF00069	0.555
MOD_GSK3_1	88	95	PF00069	0.666
MOD_N-GLC_1	248	253	PF02516	0.456
MOD_N-GLC_1	510	515	PF02516	0.482
MOD_NEK2_1	156	161	PF00069	0.453
MOD_NEK2_1	162	167	PF00069	0.423
MOD_NEK2_1	237	242	PF00069	0.372
MOD_NEK2_1	331	336	PF00069	0.375
MOD_NEK2_1	339	344	PF00069	0.346
MOD_NEK2_1	453	458	PF00069	0.764
MOD_NEK2_1	54	59	PF00069	0.567
MOD_NEK2_1	82	87	PF00069	0.668
MOD_NEK2_1	88	93	PF00069	0.669
MOD_PIKK_1	119	125	PF00454	0.525
MOD_PIKK_1	80	86	PF00454	0.666
MOD_PIKK_1	92	98	PF00454	0.530
MOD_PKA_2	101	107	PF00069	0.531
MOD_PKA_2	294	300	PF00069	0.340
MOD_PKA_2	395	401	PF00069	0.337
MOD_PKA_2	54	60	PF00069	0.626
MOD_Plk_4	266	272	PF00069	0.473
MOD_Plk_4	353	359	PF00069	0.328
MOD_Plk_4	423	429	PF00069	0.489
MOD_Plk_4	476	482	PF00069	0.577
MOD_ProDKin_1	137	143	PF00069	0.629
MOD_ProDKin_1	195	201	PF00069	0.395
MOD_ProDKin_1	207	213	PF00069	0.448
MOD_ProDKin_1	313	319	PF00069	0.337
MOD_ProDKin_1	326	332	PF00069	0.306
MOD_ProDKin_1	439	445	PF00069	0.754
MOD_ProDKin_1	455	461	PF00069	0.481
MOD_SUMO_for_1	382	385	PF00179	0.262
MOD_SUMO_rev_2	307	313	PF00179	0.398
TRG_DiLeu_BaEn_1	234	239	PF01217	0.475
TRG_DiLeu_BaEn_1	28	33	PF01217	0.418
TRG_DiLeu_BaLyEn_6	256	261	PF01217	0.437
TRG_DiLeu_BaLyEn_6	301	306	PF01217	0.350
TRG_DiLeu_BaLyEn_6	551	556	PF01217	0.441
TRG_ENDOCYTIC_2	303	306	PF00928	0.335
TRG_ENDOCYTIC_2	37	40	PF00928	0.411
TRG_ENDOCYTIC_2	540	543	PF00928	0.409
TRG_ER_diArg_1	349	351	PF00400	0.348
TRG_ER_diArg_1	428	430	PF00400	0.665
TRG_ER_diArg_1	459	462	PF00400	0.719
TRG_ER_diArg_1	498	500	PF00400	0.477
TRG_Pf-PMV_PEXEL_1	118	123	PF00026	0.552
TRG_Pf-PMV_PEXEL_1	70	74	PF00026	0.459

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P7V5	Leptomonas seymouri	64%	100%
A0A0S4JS94	Bodo saltans	29%	100%
A0A1X0P966	Trypanosomatidae	42%	100%
A0A422NK89	Trypanosoma rangeli	44%	100%
A4HNU0	Leishmania braziliensis	81%	100%
A4ICQ4	Leishmania infantum	100%	100%
D0A2Q5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	100%
E9ASK0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
Q4Q1Z6	Leishmania major	94%	100%
V5B9Y1	Trypanosoma cruzi	44%	100%