DNA-directed primase/polymerase protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

PrimPol-like protein 1, putative

Species:

Leishmania donovani

UniProt:

A0A3S5H7T6_LEIDO

TriTrypDb:

LdBPK_330030.1 , LdCL_330005200 , LDHU3_33.0040

Length:

556

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005759	mitochondrial matrix	5	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A0A3S5H7T6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S5H7T6

Function

Biological processes
Term	Name	Level	Count
GO:0000731	DNA synthesis involved in DNA repair	6	11
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006259	DNA metabolic process	4	11
GO:0006281	DNA repair	5	11
GO:0006301	postreplication repair	6	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0006950	response to stress	2	11
GO:0006974	DNA damage response	4	11
GO:0008152	metabolic process	1	11
GO:0009058	biosynthetic process	2	11
GO:0009059	macromolecule biosynthetic process	4	11
GO:0009987	cellular process	1	11
GO:0018130	heterocycle biosynthetic process	4	11
GO:0019438	aromatic compound biosynthetic process	4	11
GO:0019985	translesion synthesis	7	11
GO:0033554	cellular response to stress	3	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0034654	nucleobase-containing compound biosynthetic process	4	11
GO:0042276	error-prone translesion synthesis	8	11
GO:0043170	macromolecule metabolic process	3	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	11
GO:0044260	obsolete cellular macromolecule metabolic process	3	11
GO:0044271	cellular nitrogen compound biosynthetic process	4	11
GO:0046483	heterocycle metabolic process	3	11
GO:0050896	response to stimulus	1	11
GO:0051716	cellular response to stimulus	2	11
GO:0071704	organic substance metabolic process	2	11
GO:0071897	DNA biosynthetic process	5	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901362	organic cyclic compound biosynthetic process	4	11
GO:1901576	organic substance biosynthetic process	3	11
GO:0006260	DNA replication	5	1
GO:0006261	DNA-templated DNA replication	6	1
GO:0006264	mitochondrial DNA replication	6	1
GO:0009314	response to radiation	3	1
GO:0009411	response to UV	5	1
GO:0009416	response to light stimulus	4	1
GO:0009628	response to abiotic stimulus	2	1
GO:0031297	replication fork processing	6	1
GO:0032042	mitochondrial DNA metabolic process	5	1
GO:0045005	DNA-templated DNA replication maintenance of fidelity	5	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0003887	DNA-directed DNA polymerase activity	5	11
GO:0003896	DNA primase activity	7	11
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	11
GO:0016740	transferase activity	2	11
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	11
GO:0016779	nucleotidyltransferase activity	4	11
GO:0034061	DNA polymerase activity	4	11
GO:0034062	5'-3' RNA polymerase activity	5	11
GO:0097747	RNA polymerase activity	4	11
GO:0140097	catalytic activity, acting on DNA	3	11
GO:0140098	catalytic activity, acting on RNA	3	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:0003682	chromatin binding	2	1
GO:0005488	binding	1	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	123	127	PF00656	0.280
CLV_C14_Caspase3-7	173	177	PF00656	0.632
CLV_C14_Caspase3-7	234	238	PF00656	0.298
CLV_C14_Caspase3-7	433	437	PF00656	0.512
CLV_NRD_NRD_1	189	191	PF00675	0.578
CLV_NRD_NRD_1	204	206	PF00675	0.510
CLV_NRD_NRD_1	352	354	PF00675	0.469
CLV_NRD_NRD_1	75	77	PF00675	0.584
CLV_NRD_NRD_1	86	88	PF00675	0.660
CLV_PCSK_KEX2_1	189	191	PF00082	0.582
CLV_PCSK_KEX2_1	204	206	PF00082	0.510
CLV_PCSK_KEX2_1	352	354	PF00082	0.465
CLV_PCSK_KEX2_1	75	77	PF00082	0.576
CLV_PCSK_KEX2_1	88	90	PF00082	0.624
CLV_PCSK_PC1ET2_1	88	90	PF00082	0.624
CLV_PCSK_SKI1_1	108	112	PF00082	0.342
CLV_PCSK_SKI1_1	119	123	PF00082	0.360
CLV_PCSK_SKI1_1	190	194	PF00082	0.431
CLV_PCSK_SKI1_1	418	422	PF00082	0.516
DEG_APCC_DBOX_1	118	126	PF00400	0.472
DEG_APCC_DBOX_1	343	351	PF00400	0.399
DEG_Nend_Nbox_1	1	3	PF02207	0.409
DOC_ANK_TNKS_1	250	257	PF00023	0.410
DOC_CYCLIN_RxL_1	493	503	PF00134	0.457
DOC_MAPK_DCC_7	418	428	PF00069	0.437
DOC_MAPK_gen_1	374	382	PF00069	0.347
DOC_MAPK_gen_1	418	428	PF00069	0.437
DOC_MAPK_gen_1	515	524	PF00069	0.467
DOC_MAPK_MEF2A_6	518	526	PF00069	0.492
DOC_PP1_RVXF_1	187	194	PF00149	0.404
DOC_PP1_RVXF_1	496	503	PF00149	0.423
DOC_PP4_FxxP_1	325	328	PF00568	0.379
DOC_USP7_MATH_1	235	239	PF00917	0.379
DOC_USP7_MATH_1	307	311	PF00917	0.386
DOC_USP7_MATH_1	314	318	PF00917	0.396
DOC_WW_Pin1_4	544	549	PF00397	0.583
DOC_WW_Pin1_4	98	103	PF00397	0.517
LIG_14-3-3_CanoR_1	226	233	PF00244	0.335
LIG_14-3-3_CanoR_1	344	348	PF00244	0.487
LIG_14-3-3_CanoR_1	485	490	PF00244	0.446
LIG_14-3-3_CanoR_1	498	503	PF00244	0.511
LIG_14-3-3_CanoR_1	530	534	PF00244	0.537
LIG_BRCT_BRCA1_1	106	110	PF00533	0.493
LIG_BRCT_BRCA1_1	50	54	PF00533	0.344
LIG_Clathr_ClatBox_1	178	182	PF01394	0.436
LIG_FHA_1	173	179	PF00498	0.460
LIG_FHA_1	226	232	PF00498	0.318
LIG_FHA_1	293	299	PF00498	0.446
LIG_FHA_1	400	406	PF00498	0.511
LIG_FHA_1	430	436	PF00498	0.452
LIG_FHA_1	459	465	PF00498	0.630
LIG_FHA_1	470	476	PF00498	0.466
LIG_LIR_Apic_2	246	250	PF02991	0.412
LIG_LIR_Apic_2	544	548	PF02991	0.451
LIG_LIR_Gen_1	198	208	PF02991	0.477
LIG_LIR_Gen_1	259	267	PF02991	0.415
LIG_LIR_Gen_1	92	102	PF02991	0.533
LIG_LIR_Nem_3	198	203	PF02991	0.457
LIG_LIR_Nem_3	259	264	PF02991	0.423
LIG_LIR_Nem_3	92	97	PF02991	0.539
LIG_PCNA_yPIPBox_3	18	30	PF02747	0.339
LIG_Pex14_1	331	335	PF04695	0.428
LIG_Pex14_2	261	265	PF04695	0.390
LIG_Pex14_2	301	305	PF04695	0.446
LIG_Pex14_2	50	54	PF04695	0.344
LIG_Pex14_2	8	12	PF04695	0.343
LIG_REV1ctd_RIR_1	322	327	PF16727	0.363
LIG_SH2_CRK	479	483	PF00017	0.404
LIG_SH2_CRK	545	549	PF00017	0.531
LIG_SH2_NCK_1	479	483	PF00017	0.473
LIG_SH2_SRC	133	136	PF00017	0.375
LIG_SH2_SRC	145	148	PF00017	0.282
LIG_SH2_STAP1	479	483	PF00017	0.422
LIG_SH2_STAT5	145	148	PF00017	0.320
LIG_SH2_STAT5	283	286	PF00017	0.297
LIG_SH2_STAT5	349	352	PF00017	0.444
LIG_SH2_STAT5	387	390	PF00017	0.440
LIG_SH2_STAT5	509	512	PF00017	0.471
LIG_SH2_STAT5	523	526	PF00017	0.416
LIG_SH3_2	348	353	PF14604	0.322
LIG_SH3_3	251	257	PF00018	0.589
LIG_SH3_3	337	343	PF00018	0.404
LIG_SH3_3	345	351	PF00018	0.351
LIG_SH3_3	377	383	PF00018	0.372
LIG_SH3_3	486	492	PF00018	0.524
LIG_SUMO_SIM_anti_2	467	474	PF11976	0.374
LIG_SUMO_SIM_par_1	159	171	PF11976	0.632
LIG_TRAF2_1	171	174	PF00917	0.581
LIG_WRC_WIRS_1	236	241	PF05994	0.384
LIG_WRC_WIRS_1	499	504	PF05994	0.472
LIG_WW_1	339	342	PF00397	0.325
LIG_WW_3	248	252	PF00397	0.360
MOD_CK1_1	104	110	PF00069	0.404
MOD_CK1_1	120	126	PF00069	0.409
MOD_CK1_1	167	173	PF00069	0.601
MOD_CK1_1	294	300	PF00069	0.430
MOD_CK1_1	399	405	PF00069	0.425
MOD_CK1_1	451	457	PF00069	0.648
MOD_CK1_1	544	550	PF00069	0.326
MOD_CK1_1	58	64	PF00069	0.509
MOD_CK2_1	167	173	PF00069	0.707
MOD_CK2_1	388	394	PF00069	0.504
MOD_CK2_1	446	452	PF00069	0.651
MOD_GlcNHglycan	182	188	PF01048	0.357
MOD_GlcNHglycan	233	236	PF01048	0.346
MOD_GlcNHglycan	272	275	PF01048	0.526
MOD_GlcNHglycan	398	401	PF01048	0.551
MOD_GSK3_1	120	127	PF00069	0.448
MOD_GSK3_1	164	171	PF00069	0.662
MOD_GSK3_1	231	238	PF00069	0.423
MOD_GSK3_1	266	273	PF00069	0.434
MOD_GSK3_1	399	406	PF00069	0.446
MOD_GSK3_1	451	458	PF00069	0.710
MOD_GSK3_1	500	507	PF00069	0.330
MOD_GSK3_1	525	532	PF00069	0.442
MOD_GSK3_1	58	65	PF00069	0.530
MOD_LATS_1	496	502	PF00433	0.405
MOD_N-GLC_1	152	157	PF02516	0.573
MOD_N-GLC_2	227	229	PF02516	0.391
MOD_NEK2_1	291	296	PF00069	0.457
MOD_NEK2_1	410	415	PF00069	0.252
MOD_NEK2_1	470	475	PF00069	0.398
MOD_NEK2_1	500	505	PF00069	0.308
MOD_NEK2_2	369	374	PF00069	0.328
MOD_PIKK_1	101	107	PF00454	0.496
MOD_PIKK_1	277	283	PF00454	0.340
MOD_PIKK_1	314	320	PF00454	0.540
MOD_PIKK_1	477	483	PF00454	0.456
MOD_PKA_2	149	155	PF00069	0.477
MOD_PKA_2	225	231	PF00069	0.348
MOD_PKA_2	343	349	PF00069	0.496
MOD_PKA_2	504	510	PF00069	0.379
MOD_PKA_2	529	535	PF00069	0.471
MOD_PKB_1	241	249	PF00069	0.503
MOD_Plk_1	152	158	PF00069	0.403
MOD_Plk_1	58	64	PF00069	0.544
MOD_Plk_1	92	98	PF00069	0.666
MOD_Plk_4	174	180	PF00069	0.547
MOD_Plk_4	235	241	PF00069	0.414
MOD_Plk_4	243	249	PF00069	0.424
MOD_Plk_4	256	262	PF00069	0.459
MOD_Plk_4	376	382	PF00069	0.386
MOD_Plk_4	410	416	PF00069	0.348
MOD_Plk_4	529	535	PF00069	0.284
MOD_ProDKin_1	544	550	PF00069	0.476
MOD_ProDKin_1	98	104	PF00069	0.505
TRG_DiLeu_BaEn_1	174	179	PF01217	0.507
TRG_DiLeu_BaEn_1	59	64	PF01217	0.523
TRG_DiLeu_BaEn_1	92	97	PF01217	0.654
TRG_DiLeu_LyEn_5	59	64	PF01217	0.458
TRG_ENDOCYTIC_2	133	136	PF00928	0.353
TRG_ENDOCYTIC_2	145	148	PF00928	0.368
TRG_ENDOCYTIC_2	200	203	PF00928	0.469
TRG_ENDOCYTIC_2	332	335	PF00928	0.466
TRG_ENDOCYTIC_2	479	482	PF00928	0.441
TRG_ENDOCYTIC_2	523	526	PF00928	0.338
TRG_ER_diArg_1	188	190	PF00400	0.493
TRG_ER_diArg_1	203	205	PF00400	0.442
TRG_ER_diArg_1	351	353	PF00400	0.452
TRG_ER_diArg_1	87	90	PF00400	0.585
TRG_NLS_Bipartite_1	75	91	PF00514	0.680
TRG_NLS_MonoExtC_3	86	91	PF00514	0.586
TRG_Pf-PMV_PEXEL_1	336	341	PF00026	0.443

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PCE0	Leptomonas seymouri	57%	98%
A0A0S4IKK2	Bodo saltans	28%	83%
A0A3R7MZJ4	Trypanosoma rangeli	35%	86%
A4HL39	Leishmania braziliensis	75%	100%
A4I8L9	Leishmania infantum	99%	100%
C9ZPU3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	91%
E9B3I0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
Q4Q4M0	Leishmania major	92%	100%
V5DB07	Trypanosoma cruzi	36%	91%