Plastid-encoded RNA polymerase subunit alpha

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA-directed RNA polymerases I and III subunit RPAC1, putative

Species:

Leishmania donovani

UniProt:

A0A3S5H758_LEIDO

TriTrypDb:

LdBPK_190660.1 , LdCL_190011600 , LDHU3_19.0740

Length:

442

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000428	DNA-directed RNA polymerase complex	4	12
GO:0030880	RNA polymerase complex	3	12
GO:0032991	protein-containing complex	1	12
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	12
GO:0140535	intracellular protein-containing complex	2	12
GO:1902494	catalytic complex	2	12
GO:1990234	transferase complex	3	12
GO:0005634	nucleus	5	1
GO:0005666	RNA polymerase III complex	4	1
GO:0005730	nucleolus	5	1
GO:0005736	RNA polymerase I complex	4	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0055029	nuclear DNA-directed RNA polymerase complex	3	1
GO:0110165	cellular anatomical entity	1	1
GO:0140513	nuclear protein-containing complex	2	1

Expansion

Sequence features

A0A3S5H758

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S5H758

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006351	DNA-templated transcription	7	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009058	biosynthetic process	2	1
GO:0009059	macromolecule biosynthetic process	4	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0018130	heterocycle biosynthetic process	4	1
GO:0019438	aromatic compound biosynthetic process	4	1
GO:0032774	RNA biosynthetic process	5	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034654	nucleobase-containing compound biosynthetic process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044271	cellular nitrogen compound biosynthetic process	4	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:0097659	nucleic acid-templated transcription	6	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901362	organic cyclic compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0001054	RNA polymerase I activity	7	12
GO:0001056	RNA polymerase III activity	7	12
GO:0003824	catalytic activity	1	12
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	12
GO:0005488	binding	1	12
GO:0005515	protein binding	2	12
GO:0016740	transferase activity	2	12
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	12
GO:0016779	nucleotidyltransferase activity	4	12
GO:0034062	5'-3' RNA polymerase activity	5	12
GO:0046983	protein dimerization activity	3	12
GO:0097747	RNA polymerase activity	4	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	105	109	PF00656	0.646
CLV_C14_Caspase3-7	154	158	PF00656	0.412
CLV_NRD_NRD_1	115	117	PF00675	0.420
CLV_NRD_NRD_1	136	138	PF00675	0.212
CLV_NRD_NRD_1	5	7	PF00675	0.631
CLV_PCSK_KEX2_1	115	117	PF00082	0.420
CLV_PCSK_KEX2_1	136	138	PF00082	0.212
CLV_PCSK_KEX2_1	5	7	PF00082	0.631
CLV_PCSK_KEX2_1	73	75	PF00082	0.682
CLV_PCSK_PC1ET2_1	73	75	PF00082	0.662
CLV_PCSK_SKI1_1	136	140	PF00082	0.251
CLV_PCSK_SKI1_1	199	203	PF00082	0.241
DEG_APCC_DBOX_1	167	175	PF00400	0.423
DEG_APCC_DBOX_1	198	206	PF00400	0.455
DEG_APCC_DBOX_1	283	291	PF00400	0.412
DEG_SCF_FBW7_1	215	220	PF00400	0.482
DEG_SPOP_SBC_1	38	42	PF00917	0.677
DOC_ANK_TNKS_1	103	110	PF00023	0.651
DOC_CYCLIN_RxL_1	196	206	PF00134	0.423
DOC_MAPK_gen_1	115	122	PF00069	0.392
DOC_MAPK_gen_1	284	292	PF00069	0.412
DOC_MAPK_gen_1	3	13	PF00069	0.635
DOC_MAPK_gen_1	383	393	PF00069	0.454
DOC_PIKK_1	156	164	PF02985	0.515
DOC_PP1_RVXF_1	197	204	PF00149	0.412
DOC_PP1_RVXF_1	219	226	PF00149	0.476
DOC_USP7_MATH_1	31	35	PF00917	0.703
DOC_USP7_MATH_1	374	378	PF00917	0.406
DOC_USP7_MATH_1	433	437	PF00917	0.504
DOC_USP7_MATH_1	47	51	PF00917	0.652
DOC_USP7_MATH_1	86	90	PF00917	0.733
DOC_USP7_MATH_1	91	95	PF00917	0.719
DOC_WW_Pin1_4	125	130	PF00397	0.423
DOC_WW_Pin1_4	155	160	PF00397	0.516
DOC_WW_Pin1_4	213	218	PF00397	0.466
DOC_WW_Pin1_4	23	28	PF00397	0.551
DOC_WW_Pin1_4	300	305	PF00397	0.407
LIG_14-3-3_CanoR_1	104	108	PF00244	0.626
LIG_14-3-3_CanoR_1	119	123	PF00244	0.302
LIG_14-3-3_CanoR_1	136	146	PF00244	0.320
LIG_14-3-3_CanoR_1	53	62	PF00244	0.566
LIG_Actin_WH2_2	272	290	PF00022	0.412
LIG_Actin_WH2_2	394	410	PF00022	0.467
LIG_APCC_ABBA_1	268	273	PF00400	0.412
LIG_APCC_ABBA_1	411	416	PF00400	0.423
LIG_BIR_II_1	1	5	PF00653	0.655
LIG_BIR_III_4	206	210	PF00653	0.524
LIG_BRCT_BRCA1_1	131	135	PF00533	0.423
LIG_Clathr_ClatBox_1	202	206	PF01394	0.504
LIG_Clathr_ClatBox_1	392	396	PF01394	0.432
LIG_EVH1_2	62	66	PF00568	0.681
LIG_FHA_1	112	118	PF00498	0.439
LIG_FHA_1	138	144	PF00498	0.516
LIG_FHA_1	19	25	PF00498	0.649
LIG_FHA_1	233	239	PF00498	0.402
LIG_FHA_1	387	393	PF00498	0.466
LIG_FHA_2	103	109	PF00498	0.601
LIG_FHA_2	242	248	PF00498	0.495
LIG_FHA_2	312	318	PF00498	0.522
LIG_FHA_2	40	46	PF00498	0.673
LIG_LIR_Gen_1	145	153	PF02991	0.502
LIG_LIR_Gen_1	191	200	PF02991	0.446
LIG_LIR_Nem_3	132	138	PF02991	0.497
LIG_LIR_Nem_3	145	151	PF02991	0.468
LIG_LIR_Nem_3	191	195	PF02991	0.446
LIG_NRBOX	160	166	PF00104	0.515
LIG_PDZ_Class_2	437	442	PF00595	0.494
LIG_SH2_SRC	30	33	PF00017	0.680
LIG_SH2_STAP1	219	223	PF00017	0.498
LIG_SH2_STAT3	219	222	PF00017	0.515
LIG_SH2_STAT5	183	186	PF00017	0.437
LIG_SH3_3	124	130	PF00018	0.479
LIG_SH3_3	171	177	PF00018	0.437
LIG_SH3_3	21	27	PF00018	0.624
LIG_SH3_3	223	229	PF00018	0.507
LIG_SH3_3	349	355	PF00018	0.479
LIG_SH3_3	41	47	PF00018	0.565
MOD_CK1_1	26	32	PF00069	0.571
MOD_CK1_1	303	309	PF00069	0.453
MOD_CK1_1	34	40	PF00069	0.637
MOD_CK1_1	436	442	PF00069	0.492
MOD_CK1_1	89	95	PF00069	0.611
MOD_CK2_1	241	247	PF00069	0.441
MOD_CK2_1	311	317	PF00069	0.522
MOD_CK2_1	322	328	PF00069	0.467
MOD_GlcNHglycan	257	261	PF01048	0.360
MOD_GlcNHglycan	305	308	PF01048	0.250
MOD_GlcNHglycan	324	327	PF01048	0.276
MOD_GlcNHglycan	33	36	PF01048	0.615
MOD_GlcNHglycan	434	438	PF01048	0.531
MOD_GlcNHglycan	55	58	PF01048	0.517
MOD_GlcNHglycan	82	85	PF01048	0.667
MOD_GlcNHglycan	89	92	PF01048	0.742
MOD_GSK3_1	125	132	PF00069	0.521
MOD_GSK3_1	138	145	PF00069	0.448
MOD_GSK3_1	18	25	PF00069	0.544
MOD_GSK3_1	184	191	PF00069	0.463
MOD_GSK3_1	213	220	PF00069	0.412
MOD_GSK3_1	26	33	PF00069	0.573
MOD_GSK3_1	34	41	PF00069	0.704
MOD_GSK3_1	78	85	PF00069	0.718
MOD_GSK3_1	86	93	PF00069	0.616
MOD_GSK3_1	98	105	PF00069	0.583
MOD_N-GLC_1	125	130	PF02516	0.302
MOD_N-GLC_1	155	160	PF02516	0.237
MOD_NEK2_1	205	210	PF00069	0.512
MOD_NEK2_1	322	327	PF00069	0.534
MOD_NEK2_1	330	335	PF00069	0.490
MOD_PKA_2	103	109	PF00069	0.652
MOD_PKA_2	118	124	PF00069	0.205
MOD_PKA_2	320	326	PF00069	0.563
MOD_Plk_1	386	392	PF00069	0.463
MOD_Plk_2-3	118	124	PF00069	0.283
MOD_Plk_4	348	354	PF00069	0.467
MOD_Plk_4	91	97	PF00069	0.694
MOD_ProDKin_1	125	131	PF00069	0.423
MOD_ProDKin_1	155	161	PF00069	0.516
MOD_ProDKin_1	213	219	PF00069	0.466
MOD_ProDKin_1	23	29	PF00069	0.550
MOD_ProDKin_1	300	306	PF00069	0.407
MOD_SUMO_for_1	72	75	PF00179	0.625
MOD_SUMO_rev_2	306	315	PF00179	0.423
MOD_SUMO_rev_2	363	372	PF00179	0.415
TRG_ENDOCYTIC_2	309	312	PF00928	0.515
TRG_ER_diArg_1	115	117	PF00400	0.439
TRG_ER_diArg_1	135	137	PF00400	0.412
TRG_ER_diArg_1	381	384	PF00400	0.513
TRG_ER_diArg_1	4	6	PF00400	0.675
TRG_Pf-PMV_PEXEL_1	137	142	PF00026	0.323
TRG_Pf-PMV_PEXEL_1	150	154	PF00026	0.120
TRG_Pf-PMV_PEXEL_1	199	204	PF00026	0.223
TRG_Pf-PMV_PEXEL_1	419	423	PF00026	0.501

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8L4	Leptomonas seymouri	74%	99%
A0A0S4IYI3	Bodo saltans	45%	100%
A0A1X0NZD0	Trypanosomatidae	25%	100%
A0A1X0P6H8	Trypanosomatidae	52%	100%
A0A3R7MJ28	Trypanosoma rangeli	49%	100%
A0A3S7X218	Leishmania donovani	27%	100%
A0A422NUV2	Trypanosoma rangeli	26%	100%
A4HA24	Leishmania braziliensis	84%	100%
A4HH22	Leishmania braziliensis	27%	100%
A4HY87	Leishmania infantum	100%	100%
A4I451	Leishmania infantum	27%	100%
D0A0L6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
E9ADL6	Leishmania major	27%	100%
E9AM65	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	100%
E9AS18	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	98%
O15160	Homo sapiens	29%	100%
O94616	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	32%	100%
P07703	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	29%	100%
P16925	Tetrahymena thermophila	25%	100%
P52432	Mus musculus	30%	100%
Q32L22	Bos taurus	30%	100%
Q39211	Arabidopsis thaliana	27%	100%
Q39212	Arabidopsis thaliana	27%	100%
Q4QDF5	Leishmania major	94%	100%
Q54DH7	Dictyostelium discoideum	27%	100%
Q54SN4	Dictyostelium discoideum	28%	100%
V5BBX7	Trypanosoma cruzi	48%	100%
V5BUI6	Trypanosoma cruzi	26%	100%