Lariat debranching enzyme, C-terminal domain family protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania donovani

UniProt:

A0A3S5H711_LEIDO

TriTrypDb:

LdBPK_170930.1 , LdCL_170015300 , LDHU3_17.1350

Length:

620

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	7
GO:0043226	organelle	2	7
GO:0043227	membrane-bounded organelle	3	7
GO:0043229	intracellular organelle	3	7
GO:0043231	intracellular membrane-bounded organelle	4	7
GO:0110165	cellular anatomical entity	1	7

Expansion

Sequence features

A0A3S5H711

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S5H711

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	10
GO:0006396	RNA processing	6	10
GO:0006397	mRNA processing	7	10
GO:0006725	cellular aromatic compound metabolic process	3	10
GO:0006807	nitrogen compound metabolic process	2	10
GO:0008152	metabolic process	1	10
GO:0009987	cellular process	1	10
GO:0016070	RNA metabolic process	5	10
GO:0016071	mRNA metabolic process	6	10
GO:0034641	cellular nitrogen compound metabolic process	3	10
GO:0043170	macromolecule metabolic process	3	10
GO:0044237	cellular metabolic process	2	10
GO:0044238	primary metabolic process	2	10
GO:0046483	heterocycle metabolic process	3	10
GO:0071704	organic substance metabolic process	2	10
GO:0090304	nucleic acid metabolic process	4	10
GO:1901360	organic cyclic compound metabolic process	3	10
GO:0000375	RNA splicing, via transesterification reactions	8	1
GO:0000377	RNA splicing, via transesterification reactions with bulged adenosine as nucleophile	9	1
GO:0000398	mRNA splicing, via spliceosome	8	1
GO:0008380	RNA splicing	7	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	10
GO:0016787	hydrolase activity	2	10
GO:0016788	hydrolase activity, acting on ester bonds	3	10
GO:0004518	nuclease activity	4	6
GO:0004519	endonuclease activity	5	6
GO:0004521	RNA endonuclease activity	5	6
GO:0004540	RNA nuclease activity	4	6
GO:0008419	RNA lariat debranching enzyme activity	7	6
GO:0016891	RNA endonuclease activity, producing 5'-phosphomonoesters	6	6
GO:0016893	endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	6	6
GO:0140098	catalytic activity, acting on RNA	3	6
GO:0140640	catalytic activity, acting on a nucleic acid	2	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	591	595	PF00656	0.601
CLV_NRD_NRD_1	214	216	PF00675	0.349
CLV_NRD_NRD_1	288	290	PF00675	0.455
CLV_NRD_NRD_1	365	367	PF00675	0.368
CLV_NRD_NRD_1	392	394	PF00675	0.414
CLV_NRD_NRD_1	494	496	PF00675	0.611
CLV_PCSK_FUR_1	363	367	PF00082	0.271
CLV_PCSK_FUR_1	390	394	PF00082	0.321
CLV_PCSK_KEX2_1	214	216	PF00082	0.386
CLV_PCSK_KEX2_1	288	290	PF00082	0.455
CLV_PCSK_KEX2_1	365	367	PF00082	0.390
CLV_PCSK_KEX2_1	392	394	PF00082	0.358
CLV_PCSK_KEX2_1	494	496	PF00082	0.692
CLV_PCSK_KEX2_1	77	79	PF00082	0.413
CLV_PCSK_PC1ET2_1	77	79	PF00082	0.387
CLV_PCSK_PC7_1	73	79	PF00082	0.411
CLV_PCSK_SKI1_1	289	293	PF00082	0.420
CLV_PCSK_SKI1_1	348	352	PF00082	0.262
CLV_PCSK_SKI1_1	437	441	PF00082	0.454
CLV_Separin_Metazoa	399	403	PF03568	0.364
DEG_ODPH_VHL_1	448	461	PF01847	0.418
DEG_SPOP_SBC_1	240	244	PF00917	0.398
DEG_SPOP_SBC_1	33	37	PF00917	0.643
DEG_SPOP_SBC_1	535	539	PF00917	0.589
DEG_SPOP_SBC_1	572	576	PF00917	0.621
DOC_CYCLIN_yCln2_LP_2	292	298	PF00134	0.334
DOC_MAPK_gen_1	348	358	PF00069	0.272
DOC_MAPK_gen_1	390	400	PF00069	0.364
DOC_MAPK_gen_1	77	83	PF00069	0.478
DOC_MAPK_gen_1	92	102	PF00069	0.324
DOC_MAPK_MEF2A_6	392	400	PF00069	0.327
DOC_PP2B_LxvP_1	447	450	PF13499	0.415
DOC_PP4_FxxP_1	157	160	PF00568	0.297
DOC_PP4_FxxP_1	186	189	PF00568	0.415
DOC_PP4_FxxP_1	19	22	PF00568	0.574
DOC_USP7_MATH_1	117	121	PF00917	0.477
DOC_USP7_MATH_1	150	154	PF00917	0.324
DOC_USP7_MATH_1	206	210	PF00917	0.426
DOC_USP7_MATH_1	337	341	PF00917	0.541
DOC_USP7_MATH_1	374	378	PF00917	0.460
DOC_USP7_MATH_1	38	42	PF00917	0.648
DOC_USP7_MATH_1	488	492	PF00917	0.687
DOC_USP7_MATH_1	535	539	PF00917	0.676
DOC_USP7_UBL2_3	280	284	PF12436	0.334
DOC_WW_Pin1_4	18	23	PF00397	0.678
DOC_WW_Pin1_4	244	249	PF00397	0.634
DOC_WW_Pin1_4	36	41	PF00397	0.387
DOC_WW_Pin1_4	459	464	PF00397	0.454
DOC_WW_Pin1_4	473	478	PF00397	0.685
DOC_WW_Pin1_4	486	491	PF00397	0.759
DOC_WW_Pin1_4	536	541	PF00397	0.620
LIG_14-3-3_CanoR_1	402	408	PF00244	0.458
LIG_14-3-3_CanoR_1	503	509	PF00244	0.761
LIG_14-3-3_CanoR_1	526	531	PF00244	0.706
LIG_APCC_ABBA_1	558	563	PF00400	0.636
LIG_BIR_II_1	1	5	PF00653	0.547
LIG_BRCT_BRCA1_1	119	123	PF00533	0.345
LIG_BRCT_BRCA1_1	182	186	PF00533	0.305
LIG_BRCT_BRCA1_1	339	343	PF00533	0.321
LIG_BRCT_BRCA1_1	403	407	PF00533	0.321
LIG_BRCT_BRCA1_1	584	588	PF00533	0.576
LIG_FHA_1	134	140	PF00498	0.277
LIG_FHA_1	226	232	PF00498	0.394
LIG_FHA_1	295	301	PF00498	0.296
LIG_FHA_1	339	345	PF00498	0.258
LIG_FHA_1	380	386	PF00498	0.411
LIG_FHA_1	450	456	PF00498	0.331
LIG_FHA_1	504	510	PF00498	0.524
LIG_FHA_1	545	551	PF00498	0.711
LIG_FHA_1	553	559	PF00498	0.598
LIG_FHA_2	170	176	PF00498	0.323
LIG_FHA_2	355	361	PF00498	0.366
LIG_FHA_2	491	497	PF00498	0.674
LIG_FHA_2	589	595	PF00498	0.595
LIG_FHA_2	91	97	PF00498	0.428
LIG_GBD_Chelix_1	3	11	PF00786	0.559
LIG_IBAR_NPY_1	294	296	PF08397	0.349
LIG_LIR_Apic_2	16	22	PF02991	0.566
LIG_LIR_Apic_2	183	189	PF02991	0.349
LIG_LIR_Apic_2	190	194	PF02991	0.311
LIG_LIR_Gen_1	340	350	PF02991	0.257
LIG_LIR_Gen_1	357	364	PF02991	0.378
LIG_LIR_Gen_1	394	403	PF02991	0.331
LIG_LIR_Gen_1	87	94	PF02991	0.292
LIG_LIR_Nem_3	104	109	PF02991	0.296
LIG_LIR_Nem_3	111	115	PF02991	0.297
LIG_LIR_Nem_3	152	157	PF02991	0.280
LIG_LIR_Nem_3	340	346	PF02991	0.257
LIG_LIR_Nem_3	357	361	PF02991	0.378
LIG_LIR_Nem_3	394	400	PF02991	0.380
LIG_LIR_Nem_3	46	51	PF02991	0.346
LIG_LIR_Nem_3	87	91	PF02991	0.288
LIG_Pex14_1	44	48	PF04695	0.478
LIG_Pex14_2	279	283	PF04695	0.442
LIG_SH2_CRK	106	110	PF00017	0.435
LIG_SH2_CRK	191	195	PF00017	0.369
LIG_SH2_NCK_1	116	120	PF00017	0.400
LIG_SH2_NCK_1	191	195	PF00017	0.369
LIG_SH2_SRC	116	119	PF00017	0.385
LIG_SH2_STAP1	296	300	PF00017	0.333
LIG_SH2_STAT5	134	137	PF00017	0.304
LIG_SH2_STAT5	164	167	PF00017	0.298
LIG_SH2_STAT5	224	227	PF00017	0.401
LIG_SH2_STAT5	282	285	PF00017	0.353
LIG_SH2_STAT5	296	299	PF00017	0.348
LIG_SH2_STAT5	317	320	PF00017	0.371
LIG_SH2_STAT5	543	546	PF00017	0.641
LIG_SH3_1	191	197	PF00018	0.357
LIG_SH3_2	477	482	PF14604	0.646
LIG_SH3_3	19	25	PF00018	0.700
LIG_SH3_3	191	197	PF00018	0.357
LIG_SH3_3	319	325	PF00018	0.401
LIG_SH3_3	460	466	PF00018	0.443
LIG_SH3_3	474	480	PF00018	0.554
LIG_SH3_3	512	518	PF00018	0.644
LIG_SH3_3	596	602	PF00018	0.551
LIG_SH3_4	280	287	PF00018	0.329
LIG_SUMO_SIM_par_1	168	175	PF11976	0.340
LIG_UBA3_1	7	12	PF00899	0.612
LIG_WRC_WIRS_1	355	360	PF05994	0.335
LIG_WRPW_2	279	282	PF00400	0.360
LIG_WW_3	195	199	PF00397	0.462
LIG_WW_3	597	601	PF00397	0.634
MOD_CDC14_SPxK_1	479	482	PF00782	0.598
MOD_CDK_SPxK_1	476	482	PF00069	0.594
MOD_CK1_1	169	175	PF00069	0.399
MOD_CK1_1	233	239	PF00069	0.498
MOD_CK1_1	244	250	PF00069	0.537
MOD_CK1_1	27	33	PF00069	0.576
MOD_CK1_1	435	441	PF00069	0.545
MOD_CK1_1	476	482	PF00069	0.663
MOD_CK1_1	528	534	PF00069	0.711
MOD_CK1_1	571	577	PF00069	0.673
MOD_CK2_1	232	238	PF00069	0.487
MOD_CK2_1	40	46	PF00069	0.601
MOD_CK2_1	605	611	PF00069	0.634
MOD_CK2_1	64	70	PF00069	0.456
MOD_Cter_Amidation	75	78	PF01082	0.399
MOD_GlcNHglycan	118	122	PF01048	0.501
MOD_GlcNHglycan	13	16	PF01048	0.636
MOD_GlcNHglycan	182	185	PF01048	0.347
MOD_GlcNHglycan	208	211	PF01048	0.363
MOD_GlcNHglycan	243	246	PF01048	0.555
MOD_GlcNHglycan	26	29	PF01048	0.574
MOD_GlcNHglycan	325	328	PF01048	0.573
MOD_GlcNHglycan	36	39	PF01048	0.583
MOD_GlcNHglycan	40	43	PF01048	0.509
MOD_GlcNHglycan	437	440	PF01048	0.558
MOD_GlcNHglycan	442	445	PF01048	0.537
MOD_GlcNHglycan	490	493	PF01048	0.671
MOD_GlcNHglycan	555	558	PF01048	0.749
MOD_GlcNHglycan	570	573	PF01048	0.551
MOD_GlcNHglycan	576	579	PF01048	0.666
MOD_GSK3_1	117	124	PF00069	0.496
MOD_GSK3_1	165	172	PF00069	0.322
MOD_GSK3_1	23	30	PF00069	0.477
MOD_GSK3_1	239	246	PF00069	0.541
MOD_GSK3_1	32	39	PF00069	0.636
MOD_GSK3_1	323	330	PF00069	0.473
MOD_GSK3_1	333	340	PF00069	0.457
MOD_GSK3_1	381	388	PF00069	0.307
MOD_GSK3_1	486	493	PF00069	0.643
MOD_GSK3_1	522	529	PF00069	0.668
MOD_GSK3_1	564	571	PF00069	0.697
MOD_GSK3_1	584	591	PF00069	0.428
MOD_GSK3_1	7	14	PF00069	0.564
MOD_LATS_1	517	523	PF00433	0.691
MOD_N-GLC_1	233	238	PF02516	0.424
MOD_N-GLC_1	410	415	PF02516	0.310
MOD_N-GLC_1	503	508	PF02516	0.582
MOD_NEK2_1	165	170	PF00069	0.285
MOD_NEK2_1	225	230	PF00069	0.433
MOD_NEK2_1	333	338	PF00069	0.664
MOD_NEK2_1	386	391	PF00069	0.424
MOD_NEK2_1	401	406	PF00069	0.203
MOD_NEK2_1	541	546	PF00069	0.515
MOD_NEK2_1	610	615	PF00069	0.586
MOD_NEK2_2	133	138	PF00069	0.289
MOD_PIKK_1	225	231	PF00454	0.383
MOD_PIKK_1	544	550	PF00454	0.632
MOD_PIKK_1	611	617	PF00454	0.566
MOD_PK_1	526	532	PF00069	0.511
MOD_PKA_2	225	231	PF00069	0.383
MOD_PKA_2	401	407	PF00069	0.331
MOD_PKA_2	440	446	PF00069	0.485
MOD_PKA_2	502	508	PF00069	0.717
MOD_PKA_2	525	531	PF00069	0.544
MOD_PKA_2	72	78	PF00069	0.461
MOD_Plk_1	233	239	PF00069	0.430
MOD_Plk_1	318	324	PF00069	0.368
MOD_Plk_1	410	416	PF00069	0.300
MOD_Plk_1	495	501	PF00069	0.714
MOD_Plk_2-3	66	72	PF00069	0.363
MOD_Plk_4	133	139	PF00069	0.286
MOD_Plk_4	166	172	PF00069	0.379
MOD_Plk_4	354	360	PF00069	0.348
MOD_Plk_4	381	387	PF00069	0.382
MOD_Plk_4	451	457	PF00069	0.430
MOD_Plk_4	495	501	PF00069	0.665
MOD_Plk_4	605	611	PF00069	0.634
MOD_ProDKin_1	18	24	PF00069	0.679
MOD_ProDKin_1	244	250	PF00069	0.624
MOD_ProDKin_1	36	42	PF00069	0.375
MOD_ProDKin_1	459	465	PF00069	0.462
MOD_ProDKin_1	473	479	PF00069	0.687
MOD_ProDKin_1	486	492	PF00069	0.759
MOD_ProDKin_1	536	542	PF00069	0.621
TRG_DiLeu_BaEn_1	144	149	PF01217	0.350
TRG_DiLeu_BaLyEn_6	296	301	PF01217	0.423
TRG_ENDOCYTIC_2	106	109	PF00928	0.353
TRG_ENDOCYTIC_2	116	119	PF00928	0.324
TRG_ENDOCYTIC_2	134	137	PF00928	0.177
TRG_ENDOCYTIC_2	154	157	PF00928	0.142
TRG_ER_diArg_1	213	215	PF00400	0.347
TRG_ER_diArg_1	287	289	PF00400	0.431
TRG_ER_diArg_1	362	365	PF00400	0.364
TRG_ER_diArg_1	390	393	PF00400	0.321
TRG_ER_diArg_1	518	521	PF00400	0.765
TRG_Pf-PMV_PEXEL_1	299	303	PF00026	0.377

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HYD2	Leptomonas seymouri	52%	100%
A0A0S4ITU3	Bodo saltans	43%	100%
A0A3R7KE78	Trypanosoma rangeli	52%	100%
A4H947	Leishmania braziliensis	81%	100%
A4HXG1	Leishmania infantum	100%	100%
C9ZP39	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	46%	100%
E9AR56	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
Q4QEA7	Leishmania major	94%	100%
Q5ZLM2	Gallus gallus	34%	100%