Ring finger domain family protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ERAD-associated E3 ubiquitin-protein ligase HRD1, putative

Species:

Leishmania donovani

UniProt:

A0A3S5H6W0_LEIDO

TriTrypDb:

LdBPK_151460.1 , LdCL_150019900 , LDHU3_15.1780

Length:

478

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	10
GO:0110165	cellular anatomical entity	1	10

Expansion

Sequence features

A0A3S5H6W0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S5H6W0

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	1
GO:0006511	ubiquitin-dependent protein catabolic process	7	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0009057	macromolecule catabolic process	4	1
GO:0009987	cellular process	1	1
GO:0019538	protein metabolic process	3	1
GO:0019941	modification-dependent protein catabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043632	modification-dependent macromolecule catabolic process	5	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0044265	obsolete cellular macromolecule catabolic process	4	1
GO:0051603	proteolysis involved in protein catabolic process	5	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901575	organic substance catabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	2
GO:0004842	ubiquitin-protein transferase activity	4	2
GO:0016740	transferase activity	2	2
GO:0019787	ubiquitin-like protein transferase activity	3	2
GO:0061630	ubiquitin protein ligase activity	5	1
GO:0061659	ubiquitin-like protein ligase activity	4	1
GO:0140096	catalytic activity, acting on a protein	2	2
GO:0016746	acyltransferase activity	3	1
GO:0061631	ubiquitin conjugating enzyme activity	5	1
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	274	278	PF00656	0.558
CLV_NRD_NRD_1	269	271	PF00675	0.369
CLV_PCSK_KEX2_1	269	271	PF00082	0.385
CLV_PCSK_KEX2_1	303	305	PF00082	0.304
CLV_PCSK_KEX2_1	455	457	PF00082	0.439
CLV_PCSK_PC1ET2_1	303	305	PF00082	0.325
CLV_PCSK_PC1ET2_1	455	457	PF00082	0.422
CLV_PCSK_SKI1_1	236	240	PF00082	0.422
DEG_MDM2_SWIB_1	112	119	PF02201	0.405
DEG_SPOP_SBC_1	40	44	PF00917	0.384
DOC_CKS1_1	436	441	PF01111	0.689
DOC_MAPK_DCC_7	236	244	PF00069	0.389
DOC_MAPK_MEF2A_6	236	244	PF00069	0.389
DOC_MAPK_MEF2A_6	462	470	PF00069	0.660
DOC_MAPK_NFAT4_5	236	244	PF00069	0.389
DOC_PP2B_LxvP_1	398	401	PF13499	0.659
DOC_USP7_MATH_1	216	220	PF00917	0.418
DOC_USP7_MATH_1	40	44	PF00917	0.384
DOC_USP7_MATH_1	403	407	PF00917	0.755
DOC_USP7_MATH_1	410	414	PF00917	0.672
DOC_WW_Pin1_4	367	372	PF00397	0.811
DOC_WW_Pin1_4	421	426	PF00397	0.783
DOC_WW_Pin1_4	435	440	PF00397	0.779
LIG_14-3-3_CanoR_1	126	131	PF00244	0.284
LIG_14-3-3_CanoR_1	192	196	PF00244	0.397
LIG_14-3-3_CanoR_1	204	210	PF00244	0.333
LIG_14-3-3_CanoR_1	270	276	PF00244	0.550
LIG_14-3-3_CanoR_1	4	8	PF00244	0.527
LIG_Actin_WH2_2	177	194	PF00022	0.405
LIG_APCC_ABBA_1	94	99	PF00400	0.593
LIG_BRCT_BRCA1_1	106	110	PF00533	0.408
LIG_BRCT_BRCA1_1	222	226	PF00533	0.317
LIG_BRCT_BRCA1_1	41	45	PF00533	0.236
LIG_FHA_1	157	163	PF00498	0.520
LIG_FHA_1	256	262	PF00498	0.556
LIG_FHA_1	3	9	PF00498	0.479
LIG_FHA_1	300	306	PF00498	0.527
LIG_FHA_1	55	61	PF00498	0.325
LIG_FHA_1	76	82	PF00498	0.540
LIG_FHA_2	162	168	PF00498	0.585
LIG_FHA_2	26	32	PF00498	0.496
LIG_FHA_2	277	283	PF00498	0.577
LIG_FHA_2	377	383	PF00498	0.736
LIG_LIR_Gen_1	174	185	PF02991	0.510
LIG_LIR_Gen_1	194	203	PF02991	0.477
LIG_LIR_Gen_1	223	234	PF02991	0.305
LIG_LIR_Gen_1	263	271	PF02991	0.520
LIG_LIR_Gen_1	290	298	PF02991	0.525
LIG_LIR_Nem_3	107	112	PF02991	0.384
LIG_LIR_Nem_3	113	119	PF02991	0.328
LIG_LIR_Nem_3	174	180	PF02991	0.467
LIG_LIR_Nem_3	194	200	PF02991	0.466
LIG_LIR_Nem_3	223	229	PF02991	0.332
LIG_LIR_Nem_3	263	267	PF02991	0.529
LIG_LIR_Nem_3	28	32	PF02991	0.349
LIG_LIR_Nem_3	389	394	PF02991	0.707
LIG_LIR_Nem_3	42	48	PF02991	0.256
LIG_LIR_Nem_3	82	87	PF02991	0.601
LIG_LYPXL_S_1	32	36	PF13949	0.540
LIG_LYPXL_yS_3	33	36	PF13949	0.336
LIG_MLH1_MIPbox_1	106	110	PF16413	0.408
LIG_Pex14_2	108	112	PF04695	0.414
LIG_Pex14_2	197	201	PF04695	0.327
LIG_SH2_CRK	177	181	PF00017	0.405
LIG_SH2_CRK	183	187	PF00017	0.405
LIG_SH2_CRK	230	234	PF00017	0.408
LIG_SH2_GRB2like	25	28	PF00017	0.496
LIG_SH2_SRC	230	233	PF00017	0.462
LIG_SH2_STAP1	177	181	PF00017	0.310
LIG_SH2_STAP1	230	234	PF00017	0.450
LIG_SH2_STAP1	25	29	PF00017	0.394
LIG_SH2_STAP1	293	297	PF00017	0.528
LIG_SH2_STAT3	394	397	PF00017	0.724
LIG_SH2_STAT3	97	100	PF00017	0.603
LIG_SH2_STAT5	10	13	PF00017	0.283
LIG_SH2_STAT5	146	149	PF00017	0.328
LIG_SH2_STAT5	232	235	PF00017	0.299
LIG_SH2_STAT5	328	331	PF00017	0.460
LIG_SH2_STAT5	87	90	PF00017	0.479
LIG_SH3_3	397	403	PF00018	0.604
LIG_SH3_3	89	95	PF00018	0.528
LIG_SUMO_SIM_par_1	183	190	PF11976	0.408
LIG_SUMO_SIM_par_1	46	52	PF11976	0.348
LIG_TRAF2_1	449	452	PF00917	0.592
LIG_TRAF2_1	74	77	PF00917	0.579
LIG_TYR_ITIM	181	186	PF00017	0.423
LIG_UBA3_1	157	163	PF00899	0.477
LIG_Vh1_VBS_1	247	265	PF01044	0.515
LIG_WRC_WIRS_1	12	17	PF05994	0.375
LIG_WRC_WIRS_1	81	86	PF05994	0.583
MOD_CDC14_SPxK_1	424	427	PF00782	0.747
MOD_CDK_SPxK_1	421	427	PF00069	0.749
MOD_CK1_1	104	110	PF00069	0.414
MOD_CK1_1	190	196	PF00069	0.361
MOD_CK1_1	75	81	PF00069	0.571
MOD_CK2_1	161	167	PF00069	0.577
MOD_CK2_1	216	222	PF00069	0.405
MOD_CK2_1	25	31	PF00069	0.487
MOD_GlcNHglycan	103	106	PF01048	0.408
MOD_GlcNHglycan	138	141	PF01048	0.391
MOD_GlcNHglycan	258	261	PF01048	0.376
MOD_GlcNHglycan	340	344	PF01048	0.531
MOD_GlcNHglycan	406	409	PF01048	0.512
MOD_GlcNHglycan	421	424	PF01048	0.503
MOD_GSK3_1	121	128	PF00069	0.450
MOD_GSK3_1	129	136	PF00069	0.390
MOD_GSK3_1	171	178	PF00069	0.520
MOD_GSK3_1	186	193	PF00069	0.260
MOD_GSK3_1	216	223	PF00069	0.305
MOD_GSK3_1	404	411	PF00069	0.707
MOD_GSK3_1	415	422	PF00069	0.744
MOD_GSK3_1	426	433	PF00069	0.734
MOD_GSK3_1	434	441	PF00069	0.686
MOD_GSK3_1	75	82	PF00069	0.558
MOD_N-GLC_1	40	45	PF02516	0.522
MOD_N-GLC_1	72	77	PF02516	0.322
MOD_NEK2_1	110	115	PF00069	0.408
MOD_NEK2_1	16	21	PF00069	0.327
MOD_NEK2_1	161	166	PF00069	0.527
MOD_NEK2_1	175	180	PF00069	0.300
MOD_NEK2_1	185	190	PF00069	0.290
MOD_NEK2_1	191	196	PF00069	0.186
MOD_NEK2_1	225	230	PF00069	0.327
MOD_NEK2_1	255	260	PF00069	0.545
MOD_NEK2_1	39	44	PF00069	0.322
MOD_NEK2_1	80	85	PF00069	0.490
MOD_PKA_2	125	131	PF00069	0.283
MOD_PKA_2	191	197	PF00069	0.408
MOD_PKA_2	271	277	PF00069	0.589
MOD_PKA_2	287	293	PF00069	0.399
MOD_PKA_2	3	9	PF00069	0.527
MOD_Plk_1	40	46	PF00069	0.268
MOD_Plk_1	75	81	PF00069	0.571
MOD_Plk_4	104	110	PF00069	0.332
MOD_Plk_4	11	17	PF00069	0.259
MOD_Plk_4	133	139	PF00069	0.374
MOD_Plk_4	156	162	PF00069	0.495
MOD_Plk_4	175	181	PF00069	0.179
MOD_Plk_4	196	202	PF00069	0.351
MOD_Plk_4	205	211	PF00069	0.358
MOD_Plk_4	220	226	PF00069	0.222
MOD_Plk_4	3	9	PF00069	0.452
MOD_ProDKin_1	367	373	PF00069	0.812
MOD_ProDKin_1	421	427	PF00069	0.784
MOD_ProDKin_1	435	441	PF00069	0.771
MOD_SUMO_rev_2	167	174	PF00179	0.621
TRG_DiLeu_BaEn_1	76	81	PF01217	0.568
TRG_DiLeu_BaEn_2	221	227	PF01217	0.405
TRG_DiLeu_BaEn_4	76	82	PF01217	0.574
TRG_DiLeu_BaLyEn_6	237	242	PF01217	0.311
TRG_DiLeu_BaLyEn_6	43	48	PF01217	0.302
TRG_ENDOCYTIC_2	146	149	PF00928	0.354
TRG_ENDOCYTIC_2	177	180	PF00928	0.307
TRG_ENDOCYTIC_2	183	186	PF00928	0.260
TRG_ENDOCYTIC_2	230	233	PF00928	0.387
TRG_ENDOCYTIC_2	29	32	PF00928	0.378
TRG_ENDOCYTIC_2	293	296	PF00928	0.492
TRG_ENDOCYTIC_2	33	36	PF00928	0.330
TRG_ER_diArg_1	269	272	PF00400	0.590
TRG_Pf-PMV_PEXEL_1	455	459	PF00026	0.467

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I9A3	Leptomonas seymouri	48%	89%
A0A1X0NU29	Trypanosomatidae	35%	91%
A0A3R7MBX1	Trypanosoma rangeli	31%	89%
A4H8A9	Leishmania braziliensis	78%	100%
A4HWM9	Leishmania infantum	100%	100%
A8Y4B2	Caenorhabditis briggsae	27%	77%
C9ZXQ9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	98%
E9AQD6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
O74757	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	25%	71%
Q20798	Caenorhabditis elegans	28%	78%
Q4QF39	Leishmania major	95%	100%
Q5XHH7	Xenopus laevis	27%	80%
Q5Z880	Oryza sativa subsp. japonica	26%	96%
Q6NRL6	Xenopus laevis	28%	79%
Q803I8	Danio rerio	27%	76%
Q86TM6	Homo sapiens	28%	77%
Q95SP2	Drosophila melanogaster	29%	76%
Q9DBY1	Mus musculus	27%	78%
Q9LW77	Arabidopsis thaliana	26%	97%
V5BUE9	Trypanosoma cruzi	31%	90%