tRNA pseudouridine(55) synthase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania donovani

UniProt:

A0A3S5H6I6_LEIDO

TriTrypDb:

LdBPK_110540.1 , LdCL_110010900 , LDHU3_11.0690

Length:

720

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

A0A3S5H6I6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S5H6I6

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006399	tRNA metabolic process	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008033	tRNA processing	8	12
GO:0008152	metabolic process	1	12
GO:0009451	RNA modification	5	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0034470	ncRNA processing	7	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006400	tRNA modification	6	1
GO:0031119	tRNA pseudouridine synthesis	7	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0009982	pseudouridine synthase activity	4	12
GO:0016853	isomerase activity	2	12
GO:0016866	intramolecular transferase activity	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0106029	tRNA pseudouridine synthase activity	5	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	94	98	PF00656	0.745
CLV_NRD_NRD_1	250	252	PF00675	0.488
CLV_NRD_NRD_1	426	428	PF00675	0.410
CLV_NRD_NRD_1	562	564	PF00675	0.549
CLV_NRD_NRD_1	582	584	PF00675	0.243
CLV_NRD_NRD_1	644	646	PF00675	0.476
CLV_NRD_NRD_1	649	651	PF00675	0.388
CLV_NRD_NRD_1	82	84	PF00675	0.614
CLV_PCSK_KEX2_1	250	252	PF00082	0.694
CLV_PCSK_KEX2_1	288	290	PF00082	0.668
CLV_PCSK_KEX2_1	562	564	PF00082	0.520
CLV_PCSK_KEX2_1	582	584	PF00082	0.243
CLV_PCSK_KEX2_1	644	646	PF00082	0.476
CLV_PCSK_KEX2_1	649	651	PF00082	0.388
CLV_PCSK_KEX2_1	82	84	PF00082	0.634
CLV_PCSK_PC1ET2_1	288	290	PF00082	0.675
CLV_PCSK_PC7_1	640	646	PF00082	0.459
CLV_PCSK_SKI1_1	17	21	PF00082	0.414
CLV_PCSK_SKI1_1	179	183	PF00082	0.492
CLV_PCSK_SKI1_1	316	320	PF00082	0.742
CLV_PCSK_SKI1_1	86	90	PF00082	0.625
DEG_APCC_DBOX_1	315	323	PF00400	0.741
DEG_MDM2_SWIB_1	181	188	PF02201	0.487
DEG_SCF_FBW7_1	657	664	PF00400	0.468
DOC_ANK_TNKS_1	535	542	PF00023	0.519
DOC_CKS1_1	635	640	PF01111	0.410
DOC_MAPK_DCC_7	250	260	PF00069	0.659
DOC_MAPK_FxFP_2	32	35	PF00069	0.579
DOC_MAPK_FxFP_2	461	464	PF00069	0.425
DOC_MAPK_gen_1	582	590	PF00069	0.532
DOC_MAPK_gen_1	649	659	PF00069	0.426
DOC_MAPK_JIP1_4	542	548	PF00069	0.364
DOC_MAPK_MEF2A_6	251	260	PF00069	0.658
DOC_MAPK_MEF2A_6	530	537	PF00069	0.411
DOC_MAPK_MEF2A_6	583	592	PF00069	0.509
DOC_MAPK_MEF2A_6	689	696	PF00069	0.485
DOC_MAPK_MEF2A_6	701	709	PF00069	0.378
DOC_PP1_RVXF_1	602	609	PF00149	0.378
DOC_PP2B_LxvP_1	99	102	PF13499	0.792
DOC_PP4_FxxP_1	32	35	PF00568	0.544
DOC_PP4_FxxP_1	461	464	PF00568	0.410
DOC_PP4_FxxP_1	548	551	PF00568	0.428
DOC_USP7_MATH_1	115	119	PF00917	0.776
DOC_USP7_MATH_1	203	207	PF00917	0.557
DOC_USP7_MATH_1	240	244	PF00917	0.643
DOC_USP7_MATH_1	296	300	PF00917	0.697
DOC_USP7_MATH_1	302	306	PF00917	0.743
DOC_USP7_MATH_1	330	334	PF00917	0.605
DOC_USP7_MATH_1	409	413	PF00917	0.609
DOC_USP7_MATH_1	593	597	PF00917	0.480
DOC_WW_Pin1_4	160	165	PF00397	0.509
DOC_WW_Pin1_4	433	438	PF00397	0.400
DOC_WW_Pin1_4	473	478	PF00397	0.745
DOC_WW_Pin1_4	505	510	PF00397	0.547
DOC_WW_Pin1_4	547	552	PF00397	0.418
DOC_WW_Pin1_4	634	639	PF00397	0.405
DOC_WW_Pin1_4	657	662	PF00397	0.452
DOC_WW_Pin1_4	688	693	PF00397	0.464
DOC_WW_Pin1_4	74	79	PF00397	0.564
LIG_14-3-3_CanoR_1	179	184	PF00244	0.413
LIG_14-3-3_CanoR_1	250	258	PF00244	0.668
LIG_14-3-3_CanoR_1	27	31	PF00244	0.493
LIG_14-3-3_CanoR_1	289	298	PF00244	0.658
LIG_14-3-3_CanoR_1	341	349	PF00244	0.563
LIG_14-3-3_CanoR_1	362	369	PF00244	0.563
LIG_14-3-3_CanoR_1	406	412	PF00244	0.620
LIG_14-3-3_CanoR_1	431	440	PF00244	0.498
LIG_14-3-3_CanoR_1	555	561	PF00244	0.493
LIG_14-3-3_CanoR_1	582	590	PF00244	0.440
LIG_14-3-3_CanoR_1	594	600	PF00244	0.443
LIG_14-3-3_CanoR_1	644	648	PF00244	0.470
LIG_14-3-3_CanoR_1	652	658	PF00244	0.400
LIG_APCC_ABBA_1	258	263	PF00400	0.641
LIG_APCC_ABBAyCdc20_2	680	686	PF00400	0.391
LIG_BIR_II_1	1	5	PF00653	0.724
LIG_BIR_III_4	263	267	PF00653	0.566
LIG_BRCT_BRCA1_1	179	183	PF00533	0.382
LIG_BRCT_BRCA1_1	28	32	PF00533	0.499
LIG_BRCT_BRCA1_1	435	439	PF00533	0.414
LIG_Clathr_ClatBox_1	668	672	PF01394	0.406
LIG_CtBP_PxDLS_1	44	48	PF00389	0.509
LIG_eIF4E_1	145	151	PF01652	0.526
LIG_FHA_1	170	176	PF00498	0.565
LIG_FHA_1	250	256	PF00498	0.690
LIG_FHA_1	293	299	PF00498	0.718
LIG_FHA_1	343	349	PF00498	0.554
LIG_FHA_1	362	368	PF00498	0.492
LIG_FHA_1	506	512	PF00498	0.519
LIG_FHA_1	587	593	PF00498	0.464
LIG_FHA_1	689	695	PF00498	0.433
LIG_FHA_1	700	706	PF00498	0.341
LIG_FHA_2	196	202	PF00498	0.613
LIG_FHA_2	266	272	PF00498	0.630
LIG_FHA_2	555	561	PF00498	0.479
LIG_FHA_2	582	588	PF00498	0.448
LIG_FHA_2	92	98	PF00498	0.731
LIG_FXI_DFP_1	714	718	PF00024	0.407
LIG_LIR_Apic_2	29	35	PF02991	0.530
LIG_LIR_Apic_2	374	378	PF02991	0.403
LIG_LIR_Apic_2	547	551	PF02991	0.423
LIG_LIR_Gen_1	135	144	PF02991	0.555
LIG_LIR_Gen_1	180	189	PF02991	0.369
LIG_LIR_Gen_1	502	512	PF02991	0.631
LIG_LIR_Nem_3	135	139	PF02991	0.578
LIG_LIR_Nem_3	180	186	PF02991	0.369
LIG_LIR_Nem_3	190	195	PF02991	0.421
LIG_LIR_Nem_3	214	219	PF02991	0.531
LIG_LIR_Nem_3	502	507	PF02991	0.670
LIG_LIR_Nem_3	664	669	PF02991	0.489
LIG_MYND_1	467	471	PF01753	0.589
LIG_NRBOX	693	699	PF00104	0.399
LIG_Pex14_2	181	185	PF04695	0.488
LIG_Pex14_2	544	548	PF04695	0.393
LIG_Rb_pABgroove_1	26	34	PF01858	0.568
LIG_SH2_CRK	369	373	PF00017	0.374
LIG_SH2_CRK	375	379	PF00017	0.392
LIG_SH2_CRK	603	607	PF00017	0.374
LIG_SH2_NCK_1	369	373	PF00017	0.409
LIG_SH2_NCK_1	375	379	PF00017	0.427
LIG_SH2_SRC	261	264	PF00017	0.632
LIG_SH2_SRC	373	376	PF00017	0.396
LIG_SH2_STAP1	132	136	PF00017	0.620
LIG_SH2_STAP1	166	170	PF00017	0.630
LIG_SH2_STAP1	369	373	PF00017	0.390
LIG_SH2_STAT5	192	195	PF00017	0.560
LIG_SH2_STAT5	231	234	PF00017	0.467
LIG_SH2_STAT5	261	264	PF00017	0.559
LIG_SH2_STAT5	358	361	PF00017	0.480
LIG_SH2_STAT5	379	382	PF00017	0.424
LIG_SH2_STAT5	421	424	PF00017	0.467
LIG_SH2_STAT5	678	681	PF00017	0.386
LIG_SH2_STAT5	84	87	PF00017	0.606
LIG_SH3_2	477	482	PF14604	0.582
LIG_SH3_3	454	460	PF00018	0.425
LIG_SH3_3	461	467	PF00018	0.492
LIG_SH3_3	474	480	PF00018	0.686
LIG_SH3_3	49	55	PF00018	0.689
LIG_SUMO_SIM_par_1	491	496	PF11976	0.684
LIG_SUMO_SIM_par_1	667	673	PF11976	0.450
LIG_TRAF2_1	393	396	PF00917	0.681
LIG_TRAF2_1	449	452	PF00917	0.363
LIG_TYR_ITIM	367	372	PF00017	0.379
LIG_WRC_WIRS_1	445	450	PF05994	0.496
LIG_WW_3	35	39	PF00397	0.578
MOD_CDC14_SPxK_1	550	553	PF00782	0.446
MOD_CDK_SPK_2	74	79	PF00069	0.534
MOD_CDK_SPxK_1	547	553	PF00069	0.426
MOD_CDK_SPxK_1	634	640	PF00069	0.411
MOD_CK1_1	10	16	PF00069	0.677
MOD_CK1_1	242	248	PF00069	0.593
MOD_CK1_1	301	307	PF00069	0.751
MOD_CK1_1	328	334	PF00069	0.589
MOD_CK1_1	46	52	PF00069	0.729
MOD_CK1_1	510	516	PF00069	0.552
MOD_CK1_1	53	59	PF00069	0.645
MOD_CK1_1	567	573	PF00069	0.484
MOD_CK2_1	195	201	PF00069	0.594
MOD_CK2_1	446	452	PF00069	0.396
MOD_CK2_1	567	573	PF00069	0.497
MOD_GlcNHglycan	1	4	PF01048	0.743
MOD_GlcNHglycan	244	247	PF01048	0.585
MOD_GlcNHglycan	298	301	PF01048	0.679
MOD_GlcNHglycan	327	330	PF01048	0.691
MOD_GlcNHglycan	355	358	PF01048	0.625
MOD_GlcNHglycan	48	51	PF01048	0.724
MOD_GlcNHglycan	488	491	PF01048	0.727
MOD_GlcNHglycan	52	55	PF01048	0.698
MOD_GlcNHglycan	526	529	PF01048	0.393
MOD_GlcNHglycan	79	82	PF01048	0.474
MOD_GlcNHglycan	9	12	PF01048	0.671
MOD_GSK3_1	111	118	PF00069	0.767
MOD_GSK3_1	165	172	PF00069	0.495
MOD_GSK3_1	177	184	PF00069	0.390
MOD_GSK3_1	292	299	PF00069	0.672
MOD_GSK3_1	46	53	PF00069	0.686
MOD_GSK3_1	507	514	PF00069	0.581
MOD_GSK3_1	564	571	PF00069	0.458
MOD_GSK3_1	653	660	PF00069	0.428
MOD_GSK3_1	670	677	PF00069	0.378
MOD_N-GLC_1	177	182	PF02516	0.477
MOD_N-GLC_1	360	365	PF02516	0.568
MOD_N-GLC_1	431	436	PF02516	0.520
MOD_NEK2_1	181	186	PF00069	0.435
MOD_NEK2_1	195	200	PF00069	0.515
MOD_NEK2_1	26	31	PF00069	0.426
MOD_NEK2_1	325	330	PF00069	0.661
MOD_NEK2_1	415	420	PF00069	0.408
MOD_NEK2_1	422	427	PF00069	0.363
MOD_NEK2_1	564	569	PF00069	0.517
MOD_NEK2_1	653	658	PF00069	0.433
MOD_NEK2_1	687	692	PF00069	0.435
MOD_NEK2_2	409	414	PF00069	0.521
MOD_NEK2_2	586	591	PF00069	0.480
MOD_PIKK_1	250	256	PF00454	0.617
MOD_PIKK_1	431	437	PF00454	0.400
MOD_PIKK_1	670	676	PF00454	0.368
MOD_PKA_1	250	256	PF00069	0.481
MOD_PKA_2	249	255	PF00069	0.661
MOD_PKA_2	26	32	PF00069	0.407
MOD_PKA_2	325	331	PF00069	0.651
MOD_PKA_2	361	367	PF00069	0.590
MOD_PKA_2	401	407	PF00069	0.687
MOD_PKA_2	554	560	PF00069	0.560
MOD_PKA_2	581	587	PF00069	0.434
MOD_PKA_2	593	599	PF00069	0.452
MOD_PKA_2	643	649	PF00069	0.471
MOD_Plk_1	177	183	PF00069	0.462
MOD_Plk_1	56	62	PF00069	0.651
MOD_Plk_1	564	570	PF00069	0.506
MOD_Plk_1	586	592	PF00069	0.455
MOD_Plk_2-3	554	560	PF00069	0.524
MOD_Plk_4	132	138	PF00069	0.592
MOD_Plk_4	204	210	PF00069	0.532
MOD_Plk_4	26	32	PF00069	0.408
MOD_Plk_4	330	336	PF00069	0.596
MOD_Plk_4	367	373	PF00069	0.406
MOD_Plk_4	674	680	PF00069	0.390
MOD_ProDKin_1	160	166	PF00069	0.507
MOD_ProDKin_1	433	439	PF00069	0.402
MOD_ProDKin_1	473	479	PF00069	0.748
MOD_ProDKin_1	505	511	PF00069	0.539
MOD_ProDKin_1	547	553	PF00069	0.426
MOD_ProDKin_1	634	640	PF00069	0.411
MOD_ProDKin_1	657	663	PF00069	0.455
MOD_ProDKin_1	688	694	PF00069	0.460
MOD_ProDKin_1	74	80	PF00069	0.559
MOD_SUMO_for_1	209	212	PF00179	0.586
MOD_SUMO_rev_2	596	600	PF00179	0.549
TRG_DiLeu_BaEn_2	539	545	PF01217	0.534
TRG_DiLeu_BaLyEn_6	454	459	PF01217	0.441
TRG_DiLeu_BaLyEn_6	649	654	PF01217	0.541
TRG_ENDOCYTIC_2	145	148	PF00928	0.399
TRG_ENDOCYTIC_2	192	195	PF00928	0.560
TRG_ENDOCYTIC_2	369	372	PF00928	0.372
TRG_ENDOCYTIC_2	426	429	PF00928	0.383
TRG_ENDOCYTIC_2	603	606	PF00928	0.379
TRG_ER_diArg_1	224	227	PF00400	0.423
TRG_ER_diArg_1	249	251	PF00400	0.470
TRG_ER_diArg_1	561	563	PF00400	0.517
TRG_ER_diArg_1	581	583	PF00400	0.240
TRG_ER_diArg_1	649	652	PF00400	0.434
TRG_ER_diArg_1	82	84	PF00400	0.630
TRG_NES_CRM1_1	211	223	PF08389	0.567
TRG_NES_CRM1_1	703	716	PF08389	0.407
TRG_NLS_MonoExtN_4	285	292	PF00514	0.689
TRG_Pf-PMV_PEXEL_1	536	540	PF00026	0.498
TRG_Pf-PMV_PEXEL_1	611	615	PF00026	0.446
TRG_Pf-PMV_PEXEL_1	629	634	PF00026	0.535

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HW55	Leptomonas seymouri	67%	96%
A0A0S4IV39	Bodo saltans	37%	90%
A0A1X0NVE2	Trypanosomatidae	44%	100%
A0A422MV99	Trypanosoma rangeli	45%	100%
A4H6H8	Leishmania braziliensis	83%	100%
A4HUW4	Leishmania infantum	100%	100%
D0A7I9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
E9ANJ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
Q4QH27	Leishmania major	95%	100%
V5BW97	Trypanosoma cruzi	45%	100%