EF-hand domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Protein of unknown function (DUF2453), putative

Species:

Leishmania donovani

UniProt:

A0A3S5H678_LEIDO

TriTrypDb:

LdBPK_080910.1 , LdCL_080014500 , LDHU3_08.1260

Length:

833

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 5
NetGPI	no	yes: 0, no: 5

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	6
GO:0110165	cellular anatomical entity	1	6
GO:0005739	mitochondrion	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1

Expansion

Sequence features

A0A3S5H678

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S5H678

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	6
GO:0005509	calcium ion binding	5	6
GO:0043167	ion binding	2	6
GO:0043169	cation binding	3	6
GO:0046872	metal ion binding	4	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	200	204	PF00656	0.543
CLV_C14_Caspase3-7	263	267	PF00656	0.483
CLV_C14_Caspase3-7	440	444	PF00656	0.551
CLV_C14_Caspase3-7	580	584	PF00656	0.476
CLV_NRD_NRD_1	2	4	PF00675	0.565
CLV_NRD_NRD_1	33	35	PF00675	0.764
CLV_NRD_NRD_1	346	348	PF00675	0.697
CLV_NRD_NRD_1	411	413	PF00675	0.692
CLV_NRD_NRD_1	456	458	PF00675	0.731
CLV_NRD_NRD_1	466	468	PF00675	0.738
CLV_PCSK_FUR_1	379	383	PF00082	0.722
CLV_PCSK_KEX2_1	2	4	PF00082	0.571
CLV_PCSK_KEX2_1	33	35	PF00082	0.761
CLV_PCSK_KEX2_1	346	348	PF00082	0.678
CLV_PCSK_KEX2_1	381	383	PF00082	0.762
CLV_PCSK_KEX2_1	411	413	PF00082	0.701
CLV_PCSK_KEX2_1	456	458	PF00082	0.758
CLV_PCSK_KEX2_1	465	467	PF00082	0.720
CLV_PCSK_PC1ET2_1	381	383	PF00082	0.762
CLV_PCSK_PC1ET2_1	465	467	PF00082	0.797
CLV_PCSK_PC7_1	377	383	PF00082	0.750
CLV_PCSK_SKI1_1	118	122	PF00082	0.747
CLV_PCSK_SKI1_1	254	258	PF00082	0.762
CLV_PCSK_SKI1_1	360	364	PF00082	0.752
CLV_PCSK_SKI1_1	411	415	PF00082	0.750
CLV_PCSK_SKI1_1	467	471	PF00082	0.826
CLV_PCSK_SKI1_1	823	827	PF00082	0.484
DEG_APCC_DBOX_1	117	125	PF00400	0.517
DEG_APCC_DBOX_1	410	418	PF00400	0.480
DEG_Nend_UBRbox_1	1	4	PF02207	0.741
DEG_SCF_FBW7_1	24	30	PF00400	0.565
DEG_SCF_SKP2-CKS1_1	458	465	PF00560	0.533
DEG_SPOP_SBC_1	218	222	PF00917	0.523
DEG_SPOP_SBC_1	279	283	PF00917	0.560
DEG_SPOP_SBC_1	349	353	PF00917	0.576
DEG_SPOP_SBC_1	48	52	PF00917	0.484
DEG_SPOP_SBC_1	64	68	PF00917	0.433
DEG_SPOP_SBC_1	82	86	PF00917	0.495
DOC_ANK_TNKS_1	581	588	PF00023	0.481
DOC_CDC14_PxL_1	764	772	PF14671	0.284
DOC_CKS1_1	24	29	PF01111	0.564
DOC_MAPK_DCC_7	730	738	PF00069	0.329
DOC_MAPK_gen_1	545	552	PF00069	0.565
DOC_MAPK_MEF2A_6	730	738	PF00069	0.329
DOC_USP7_MATH_1	104	108	PF00917	0.445
DOC_USP7_MATH_1	135	139	PF00917	0.472
DOC_USP7_MATH_1	187	191	PF00917	0.553
DOC_USP7_MATH_1	248	252	PF00917	0.597
DOC_USP7_MATH_1	259	263	PF00917	0.516
DOC_USP7_MATH_1	264	268	PF00917	0.481
DOC_USP7_MATH_1	271	275	PF00917	0.534
DOC_USP7_MATH_1	279	283	PF00917	0.491
DOC_USP7_MATH_1	300	304	PF00917	0.578
DOC_USP7_MATH_1	324	328	PF00917	0.605
DOC_USP7_MATH_1	333	337	PF00917	0.511
DOC_USP7_MATH_1	349	353	PF00917	0.538
DOC_USP7_MATH_1	43	47	PF00917	0.494
DOC_USP7_MATH_1	484	488	PF00917	0.541
DOC_USP7_MATH_1	508	512	PF00917	0.628
DOC_USP7_MATH_1	527	531	PF00917	0.462
DOC_USP7_MATH_1	537	541	PF00917	0.553
DOC_USP7_MATH_1	59	63	PF00917	0.494
DOC_USP7_MATH_1	594	598	PF00917	0.537
DOC_USP7_MATH_1	613	617	PF00917	0.498
DOC_USP7_MATH_1	635	639	PF00917	0.550
DOC_USP7_MATH_1	64	68	PF00917	0.530
DOC_USP7_MATH_1	646	650	PF00917	0.483
DOC_USP7_MATH_1	705	709	PF00917	0.426
DOC_USP7_MATH_1	793	797	PF00917	0.695
DOC_USP7_MATH_1	82	86	PF00917	0.518
DOC_USP7_MATH_1	95	99	PF00917	0.571
DOC_USP7_UBL2_3	802	806	PF12436	0.632
DOC_WW_Pin1_4	185	190	PF00397	0.499
DOC_WW_Pin1_4	23	28	PF00397	0.311
DOC_WW_Pin1_4	269	274	PF00397	0.544
DOC_WW_Pin1_4	275	280	PF00397	0.508
DOC_WW_Pin1_4	298	303	PF00397	0.564
DOC_WW_Pin1_4	325	330	PF00397	0.558
DOC_WW_Pin1_4	351	356	PF00397	0.503
DOC_WW_Pin1_4	371	376	PF00397	0.499
DOC_WW_Pin1_4	459	464	PF00397	0.553
DOC_WW_Pin1_4	55	60	PF00397	0.567
DOC_WW_Pin1_4	566	571	PF00397	0.456
DOC_WW_Pin1_4	599	604	PF00397	0.560
DOC_WW_Pin1_4	628	633	PF00397	0.563
DOC_WW_Pin1_4	93	98	PF00397	0.491
LIG_14-3-3_CanoR_1	10	19	PF00244	0.538
LIG_14-3-3_CanoR_1	34	44	PF00244	0.580
LIG_14-3-3_CanoR_1	360	369	PF00244	0.572
LIG_14-3-3_CanoR_1	545	552	PF00244	0.497
LIG_14-3-3_CanoR_1	605	609	PF00244	0.513
LIG_14-3-3_CanoR_1	735	739	PF00244	0.356
LIG_14-3-3_CanoR_1	748	756	PF00244	0.273
LIG_14-3-3_CanoR_1	799	805	PF00244	0.626
LIG_BIR_III_4	622	626	PF00653	0.538
LIG_BRCT_BRCA1_1	285	289	PF00533	0.475
LIG_BRCT_BRCA1_1	420	424	PF00533	0.483
LIG_EVH1_2	174	178	PF00568	0.499
LIG_FHA_1	112	118	PF00498	0.491
LIG_FHA_1	179	185	PF00498	0.597
LIG_FHA_1	241	247	PF00498	0.521
LIG_FHA_1	338	344	PF00498	0.558
LIG_FHA_1	558	564	PF00498	0.463
LIG_FHA_1	681	687	PF00498	0.567
LIG_FHA_2	186	192	PF00498	0.554
LIG_FHA_2	261	267	PF00498	0.484
LIG_FHA_2	438	444	PF00498	0.509
LIG_GBD_Chelix_1	384	392	PF00786	0.707
LIG_LIR_Gen_1	20	29	PF02991	0.311
LIG_LIR_Gen_1	286	297	PF02991	0.481
LIG_LIR_Gen_1	427	435	PF02991	0.531
LIG_LIR_Gen_1	661	670	PF02991	0.367
LIG_LIR_Nem_3	20	25	PF02991	0.311
LIG_LIR_Nem_3	421	426	PF02991	0.556
LIG_LIR_Nem_3	427	431	PF02991	0.503
LIG_LIR_Nem_3	631	636	PF02991	0.517
LIG_LIR_Nem_3	661	665	PF02991	0.426
LIG_LIR_Nem_3	79	83	PF02991	0.460
LIG_MAD2	664	672	PF02301	0.437
LIG_MLH1_MIPbox_1	420	424	PF16413	0.483
LIG_MYND_1	396	400	PF01753	0.497
LIG_NRBOX	387	393	PF00104	0.508
LIG_PTAP_UEV_1	96	101	PF05743	0.463
LIG_RPA_C_Fungi	612	624	PF08784	0.677
LIG_SH2_NCK_1	102	106	PF00017	0.587
LIG_SH2_NCK_1	428	432	PF00017	0.627
LIG_SH2_NCK_1	728	732	PF00017	0.437
LIG_SH2_NCK_1	746	750	PF00017	0.223
LIG_SH2_SRC	428	431	PF00017	0.630
LIG_SH2_STAP1	428	432	PF00017	0.627
LIG_SH2_STAP1	451	455	PF00017	0.596
LIG_SH2_STAP1	728	732	PF00017	0.382
LIG_SH2_STAT5	102	105	PF00017	0.594
LIG_SH2_STAT5	322	325	PF00017	0.583
LIG_SH2_STAT5	423	426	PF00017	0.622
LIG_SH2_STAT5	656	659	PF00017	0.542
LIG_SH2_STAT5	662	665	PF00017	0.449
LIG_SH2_STAT5	728	731	PF00017	0.437
LIG_SH3_3	282	288	PF00018	0.635
LIG_SH3_3	478	484	PF00018	0.592
LIG_SH3_3	499	505	PF00018	0.732
LIG_SH3_3	53	59	PF00018	0.675
LIG_SH3_3	629	635	PF00018	0.769
LIG_SH3_3	91	97	PF00018	0.610
LIG_Sin3_3	234	241	PF02671	0.700
LIG_TRAF2_1	779	782	PF00917	0.630
LIG_TYR_ITIM	426	431	PF00017	0.639
LIG_UBA3_1	562	568	PF00899	0.656
LIG_WW_3	732	736	PF00397	0.437
MOD_CDC14_SPxK_1	374	377	PF00782	0.688
MOD_CDC14_SPxK_1	602	605	PF00782	0.680
MOD_CDK_SPK_2	351	356	PF00069	0.599
MOD_CDK_SPxK_1	371	377	PF00069	0.699
MOD_CDK_SPxK_1	459	465	PF00069	0.677
MOD_CDK_SPxK_1	599	605	PF00069	0.679
MOD_CDK_SPxxK_3	298	305	PF00069	0.680
MOD_CDK_SPxxK_3	459	466	PF00069	0.677
MOD_CK1_1	106	112	PF00069	0.533
MOD_CK1_1	179	185	PF00069	0.699
MOD_CK1_1	194	200	PF00069	0.681
MOD_CK1_1	214	220	PF00069	0.591
MOD_CK1_1	272	278	PF00069	0.734
MOD_CK1_1	280	286	PF00069	0.675
MOD_CK1_1	328	334	PF00069	0.715
MOD_CK1_1	336	342	PF00069	0.583
MOD_CK1_1	351	357	PF00069	0.622
MOD_CK1_1	394	400	PF00069	0.560
MOD_CK1_1	407	413	PF00069	0.608
MOD_CK1_1	445	451	PF00069	0.606
MOD_CK1_1	475	481	PF00069	0.721
MOD_CK1_1	503	509	PF00069	0.831
MOD_CK1_1	569	575	PF00069	0.718
MOD_CK1_1	601	607	PF00069	0.745
MOD_CK1_1	638	644	PF00069	0.621
MOD_CK1_1	747	753	PF00069	0.437
MOD_CK1_1	773	779	PF00069	0.615
MOD_CK1_1	84	90	PF00069	0.620
MOD_CK1_1	98	104	PF00069	0.613
MOD_CK2_1	106	112	PF00069	0.611
MOD_CK2_1	122	128	PF00069	0.630
MOD_CK2_1	149	155	PF00069	0.660
MOD_CK2_1	179	185	PF00069	0.697
MOD_CK2_1	300	306	PF00069	0.676
MOD_CK2_1	444	450	PF00069	0.618
MOD_GlcNHglycan	106	109	PF01048	0.570
MOD_GlcNHglycan	137	140	PF01048	0.571
MOD_GlcNHglycan	144	147	PF01048	0.537
MOD_GlcNHglycan	169	172	PF01048	0.703
MOD_GlcNHglycan	178	181	PF01048	0.609
MOD_GlcNHglycan	194	197	PF01048	0.654
MOD_GlcNHglycan	199	202	PF01048	0.777
MOD_GlcNHglycan	203	207	PF01048	0.728
MOD_GlcNHglycan	216	219	PF01048	0.605
MOD_GlcNHglycan	249	253	PF01048	0.724
MOD_GlcNHglycan	266	269	PF01048	0.624
MOD_GlcNHglycan	285	288	PF01048	0.496
MOD_GlcNHglycan	302	305	PF01048	0.675
MOD_GlcNHglycan	331	334	PF01048	0.708
MOD_GlcNHglycan	335	338	PF01048	0.729
MOD_GlcNHglycan	40	43	PF01048	0.685
MOD_GlcNHglycan	406	409	PF01048	0.616
MOD_GlcNHglycan	45	48	PF01048	0.657
MOD_GlcNHglycan	474	477	PF01048	0.696
MOD_GlcNHglycan	484	487	PF01048	0.657
MOD_GlcNHglycan	521	524	PF01048	0.700
MOD_GlcNHglycan	531	534	PF01048	0.654
MOD_GlcNHglycan	539	542	PF01048	0.603
MOD_GlcNHglycan	571	574	PF01048	0.700
MOD_GlcNHglycan	609	613	PF01048	0.804
MOD_GlcNHglycan	61	64	PF01048	0.596
MOD_GlcNHglycan	637	640	PF01048	0.655
MOD_GlcNHglycan	643	646	PF01048	0.630
MOD_GlcNHglycan	695	698	PF01048	0.513
MOD_GlcNHglycan	713	716	PF01048	0.446
MOD_GlcNHglycan	772	775	PF01048	0.595
MOD_GlcNHglycan	97	100	PF01048	0.625
MOD_GSK3_1	100	107	PF00069	0.571
MOD_GSK3_1	135	142	PF00069	0.617
MOD_GSK3_1	187	194	PF00069	0.704
MOD_GSK3_1	202	209	PF00069	0.599
MOD_GSK3_1	210	217	PF00069	0.656
MOD_GSK3_1	218	225	PF00069	0.554
MOD_GSK3_1	23	30	PF00069	0.606
MOD_GSK3_1	260	267	PF00069	0.788
MOD_GSK3_1	271	278	PF00069	0.593
MOD_GSK3_1	279	286	PF00069	0.659
MOD_GSK3_1	318	325	PF00069	0.757
MOD_GSK3_1	329	336	PF00069	0.645
MOD_GSK3_1	351	358	PF00069	0.755
MOD_GSK3_1	363	370	PF00069	0.646
MOD_GSK3_1	43	50	PF00069	0.659
MOD_GSK3_1	500	507	PF00069	0.775
MOD_GSK3_1	537	544	PF00069	0.708
MOD_GSK3_1	55	62	PF00069	0.580
MOD_GSK3_1	594	601	PF00069	0.679
MOD_GSK3_1	604	611	PF00069	0.625
MOD_GSK3_1	634	641	PF00069	0.704
MOD_GSK3_1	695	702	PF00069	0.522
MOD_GSK3_1	72	79	PF00069	0.532
MOD_GSK3_1	740	747	PF00069	0.436
MOD_GSK3_1	89	96	PF00069	0.599
MOD_N-GLC_1	147	152	PF02516	0.650
MOD_N-GLC_1	210	215	PF02516	0.666
MOD_N-GLC_1	613	618	PF02516	0.723
MOD_N-GLC_1	680	685	PF02516	0.442
MOD_NEK2_1	149	154	PF00069	0.747
MOD_NEK2_1	17	22	PF00069	0.391
MOD_NEK2_1	178	183	PF00069	0.743
MOD_NEK2_1	458	463	PF00069	0.651
MOD_NEK2_1	608	613	PF00069	0.632
MOD_NEK2_1	679	684	PF00069	0.454
MOD_NEK2_1	695	700	PF00069	0.331
MOD_NEK2_1	770	775	PF00069	0.606
MOD_NEK2_2	646	651	PF00069	0.624
MOD_PIKK_1	212	218	PF00454	0.635
MOD_PIKK_1	305	311	PF00454	0.735
MOD_PIKK_1	506	512	PF00454	0.616
MOD_PIKK_1	699	705	PF00454	0.437
MOD_PIKK_1	747	753	PF00454	0.522
MOD_PIKK_1	773	779	PF00454	0.623
MOD_PK_1	442	448	PF00069	0.631
MOD_PKA_2	292	298	PF00069	0.616
MOD_PKA_2	367	373	PF00069	0.723
MOD_PKA_2	604	610	PF00069	0.688
MOD_PKA_2	726	732	PF00069	0.437
MOD_PKA_2	734	740	PF00069	0.342
MOD_PKA_2	747	753	PF00069	0.319
MOD_Plk_1	442	448	PF00069	0.631
MOD_Plk_1	679	685	PF00069	0.437
MOD_Plk_1	807	813	PF00069	0.522
MOD_Plk_2-3	240	246	PF00069	0.709
MOD_Plk_4	222	228	PF00069	0.737
MOD_Plk_4	318	324	PF00069	0.585
MOD_Plk_4	658	664	PF00069	0.523
MOD_Plk_4	680	686	PF00069	0.437
MOD_Plk_4	89	95	PF00069	0.675
MOD_ProDKin_1	185	191	PF00069	0.623
MOD_ProDKin_1	23	29	PF00069	0.311
MOD_ProDKin_1	269	275	PF00069	0.682
MOD_ProDKin_1	298	304	PF00069	0.708
MOD_ProDKin_1	325	331	PF00069	0.702
MOD_ProDKin_1	351	357	PF00069	0.623
MOD_ProDKin_1	371	377	PF00069	0.615
MOD_ProDKin_1	459	465	PF00069	0.702
MOD_ProDKin_1	55	61	PF00069	0.709
MOD_ProDKin_1	566	572	PF00069	0.563
MOD_ProDKin_1	599	605	PF00069	0.707
MOD_ProDKin_1	628	634	PF00069	0.710
MOD_ProDKin_1	93	99	PF00069	0.605
MOD_SUMO_for_1	130	133	PF00179	0.611
TRG_DiLeu_BaEn_1	409	414	PF01217	0.605
TRG_DiLeu_BaLyEn_6	765	770	PF01217	0.437
TRG_DiLeu_LyEn_5	409	414	PF01217	0.605
TRG_ENDOCYTIC_2	423	426	PF00928	0.622
TRG_ENDOCYTIC_2	428	431	PF00928	0.570
TRG_ENDOCYTIC_2	662	665	PF00928	0.521
TRG_ER_diArg_1	1	3	PF00400	0.732
TRG_ER_diArg_1	376	379	PF00400	0.717
TRG_ER_diArg_1	411	413	PF00400	0.609
TRG_ER_diArg_1	455	457	PF00400	0.675
TRG_ER_diArg_1	811	814	PF00400	0.538
TRG_NLS_MonoCore_2	229	234	PF00514	0.702
TRG_NLS_MonoExtN_4	228	234	PF00514	0.707
TRG_NLS_MonoExtN_4	463	469	PF00514	0.711
TRG_Pf-PMV_PEXEL_1	122	126	PF00026	0.664

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A4H5E2	Leishmania braziliensis	68%	99%
A4HTN7	Leishmania infantum	100%	100%
E9AMH1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	97%
Q4QI72	Leishmania major	88%	100%