Eukaryotic translation initiation factor 2 beta subunit, putative

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

translation initiation factor-like protein

Species:

Leishmania donovani

UniProt:

A0A3S5H658_LEIDO

TriTrypDb:

LdBPK_080570.1 , LdCL_080010600 , LDHU3_08.0630

Length:

345

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 6

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0005850	eukaryotic translation initiation factor 2 complex	2	1
GO:0032991	protein-containing complex	1	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A0A3S5H658

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S5H658

Function

Biological processes
Term	Name	Level	Count
GO:0001731	formation of translation preinitiation complex	7	1
GO:0001732	formation of cytoplasmic translation initiation complex	7	1
GO:0009987	cellular process	1	1
GO:0016043	cellular component organization	3	1
GO:0022607	cellular component assembly	4	1
GO:0022618	ribonucleoprotein complex assembly	6	1
GO:0043933	protein-containing complex organization	4	1
GO:0065003	protein-containing complex assembly	5	1
GO:0071826	ribonucleoprotein complex subunit organization	5	1
GO:0071840	cellular component organization or biogenesis	2	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003743	translation initiation factor activity	4	12
GO:0005488	binding	1	12
GO:0008135	translation factor activity, RNA binding	3	12
GO:0045182	translation regulator activity	1	12
GO:0090079	translation regulator activity, nucleic acid binding	2	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0003723	RNA binding	4	1
GO:0003729	mRNA binding	5	1
GO:0005515	protein binding	2	1
GO:0031369	translation initiation factor binding	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	10	12	PF00675	0.756
CLV_NRD_NRD_1	271	273	PF00675	0.537
CLV_NRD_NRD_1	306	308	PF00675	0.429
CLV_PCSK_FUR_1	7	11	PF00082	0.764
CLV_PCSK_KEX2_1	306	308	PF00082	0.429
CLV_PCSK_KEX2_1	335	337	PF00082	0.556
CLV_PCSK_KEX2_1	9	11	PF00082	0.732
CLV_PCSK_PC1ET2_1	335	337	PF00082	0.556
CLV_PCSK_SKI1_1	319	323	PF00082	0.565
DEG_Nend_Nbox_1	1	3	PF02207	0.643
DEG_SPOP_SBC_1	161	165	PF00917	0.632
DOC_MAPK_gen_1	335	345	PF00069	0.589
DOC_MAPK_MEF2A_6	210	217	PF00069	0.500
DOC_PP2B_LxvP_1	213	216	PF13499	0.471
DOC_PP4_FxxP_1	238	241	PF00568	0.392
DOC_USP7_MATH_1	162	166	PF00917	0.712
DOC_USP7_MATH_1	172	176	PF00917	0.740
DOC_USP7_MATH_1	51	55	PF00917	0.815
DOC_USP7_MATH_1	97	101	PF00917	0.703
LIG_14-3-3_CanoR_1	186	191	PF00244	0.529
LIG_14-3-3_CanoR_1	272	281	PF00244	0.532
LIG_APCC_ABBA_1	58	63	PF00400	0.782
LIG_BRCT_BRCA1_1	99	103	PF00533	0.569
LIG_FHA_1	26	32	PF00498	0.877
LIG_FHA_1	316	322	PF00498	0.482
LIG_FHA_2	273	279	PF00498	0.392
LIG_FHA_2	28	34	PF00498	0.923
LIG_FHA_2	78	84	PF00498	0.718
LIG_LIR_Apic_2	236	241	PF02991	0.471
LIG_LIR_Gen_1	111	120	PF02991	0.598
LIG_LIR_Gen_1	156	162	PF02991	0.694
LIG_LIR_Gen_1	275	285	PF02991	0.435
LIG_LIR_Nem_3	111	115	PF02991	0.660
LIG_LIR_Nem_3	116	120	PF02991	0.594
LIG_LIR_Nem_3	156	161	PF02991	0.660
LIG_LIR_Nem_3	192	198	PF02991	0.610
LIG_LIR_Nem_3	275	280	PF02991	0.417
LIG_MYND_3	215	219	PF01753	0.519
LIG_PCNA_yPIPBox_3	186	195	PF02747	0.648
LIG_SH2_CRK	112	116	PF00017	0.658
LIG_SH2_CRK	117	121	PF00017	0.577
LIG_SH2_STAP1	117	121	PF00017	0.624
LIG_SH2_STAT5	284	287	PF00017	0.377
LIG_SH2_STAT5	288	291	PF00017	0.377
LIG_SH3_3	50	56	PF00018	0.787
LIG_SH3_3	66	72	PF00018	0.730
LIG_TRAF2_1	131	134	PF00917	0.596
LIG_TRAF2_1	177	180	PF00917	0.680
LIG_TRAF2_1	30	33	PF00917	0.803
LIG_TYR_ITIM	110	115	PF00017	0.643
LIG_UBA3_1	217	225	PF00899	0.519
LIG_UBA3_1	281	290	PF00899	0.435
MOD_CK1_1	15	21	PF00069	0.929
MOD_CK1_1	205	211	PF00069	0.379
MOD_CK1_1	54	60	PF00069	0.867
MOD_CK1_1	73	79	PF00069	0.781
MOD_CK2_1	15	21	PF00069	0.816
MOD_CK2_1	172	178	PF00069	0.756
MOD_CK2_1	27	33	PF00069	0.795
MOD_CK2_1	77	83	PF00069	0.755
MOD_Cter_Amidation	221	224	PF01082	0.412
MOD_Cter_Amidation	304	307	PF01082	0.392
MOD_GlcNHglycan	165	168	PF01048	0.688
MOD_GlcNHglycan	174	177	PF01048	0.667
MOD_GlcNHglycan	203	207	PF01048	0.600
MOD_GlcNHglycan	329	332	PF01048	0.577
MOD_GlcNHglycan	53	56	PF01048	0.821
MOD_GlcNHglycan	72	75	PF01048	0.759
MOD_GlcNHglycan	92	95	PF01048	0.823
MOD_GSK3_1	12	19	PF00069	0.909
MOD_GSK3_1	27	34	PF00069	0.624
MOD_GSK3_1	313	320	PF00069	0.394
MOD_GSK3_1	323	330	PF00069	0.606
MOD_GSK3_1	73	80	PF00069	0.754
MOD_GSK3_1	97	104	PF00069	0.711
MOD_N-GLC_1	172	177	PF02516	0.742
MOD_N-GLC_2	292	294	PF02516	0.392
MOD_N-GLC_2	313	315	PF02516	0.377
MOD_NEK2_2	56	61	PF00069	0.713
MOD_PIKK_1	64	70	PF00454	0.723
MOD_PK_1	251	257	PF00069	0.392
MOD_PKA_1	223	229	PF00069	0.519
MOD_PKA_1	272	278	PF00069	0.519
MOD_PKA_2	125	131	PF00069	0.604
MOD_Plk_1	143	149	PF00069	0.678
MOD_Plk_1	251	257	PF00069	0.391
MOD_Plk_1	297	303	PF00069	0.377
MOD_Plk_2-3	17	23	PF00069	0.827
MOD_Plk_4	186	192	PF00069	0.539
MOD_Plk_4	223	229	PF00069	0.519
MOD_SUMO_for_1	267	270	PF00179	0.392
MOD_SUMO_rev_2	243	248	PF00179	0.519
TRG_ENDOCYTIC_2	112	115	PF00928	0.622
TRG_ENDOCYTIC_2	117	120	PF00928	0.563
TRG_ENDOCYTIC_2	187	190	PF00928	0.500
TRG_ER_diArg_1	306	308	PF00400	0.428
TRG_ER_diArg_1	7	10	PF00400	0.760
TRG_NLS_MonoExtN_4	220	227	PF00514	0.392
TRG_Pf-PMV_PEXEL_1	283	287	PF00026	0.392

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I3Y9	Leptomonas seymouri	72%	99%
A0A0S4KLD6	Bodo saltans	39%	100%
A0A1X0NPQ0	Trypanosomatidae	50%	91%
A0A3R7JYX9	Trypanosoma rangeli	51%	100%
A4H5C4	Leishmania braziliensis	86%	99%
A4HTK4	Leishmania infantum	100%	100%
C9ZPG8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	59%	100%
E9AMD5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
Q4QIB4	Leishmania major	98%	100%
V5BGW0	Trypanosoma cruzi	52%	100%