Electron transfer flavoprotein-ubiquinone oxidoreductase

Quick info Annotations Localization Abundance Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

electron transfer flavoprotein-ubiquinone oxidoreductase, putative

Species:

Leishmania donovani

UniProt:

A0A3S5H5Y7_LEIDO

TriTrypDb:

LdBPK_070660.1 , LdCL_070011500 , LDHU3_07.0800

Length:

575

Annotations

Annotations by Jardim et al.

Electron Transport Chain (see tricarboxylic acid section for Complex II), electron transfer flavo-ubiquinone oxidoreductase

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005743	mitochondrial inner membrane	5	11
GO:0016020	membrane	2	11
GO:0019866	organelle inner membrane	4	11
GO:0031090	organelle membrane	3	11
GO:0031966	mitochondrial membrane	4	11
GO:0110165	cellular anatomical entity	1	11

Abundance

Amastigote (protein)


Source	Evidence on protein	Close homologs
Pescher et al. (upgregulation)	no	yes: 0

Promastigote and amastigote


Source	Evidence on protein	Close homologs
Lahav et al. - mRNA - Protein

Expansion

Sequence features

A0A3S5H5Y7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S5H5Y7

Function

Biological processes
Term	Name	Level	Count
GO:0006091	generation of precursor metabolites and energy	3	1
GO:0008152	metabolic process	1	1
GO:0009987	cellular process	1	1
GO:0022900	electron transport chain	4	1
GO:0044237	cellular metabolic process	2	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0004174	electron-transferring-flavoprotein dehydrogenase activity	4	12
GO:0005488	binding	1	12
GO:0009055	electron transfer activity	3	12
GO:0016491	oxidoreductase activity	2	12
GO:0016645	oxidoreductase activity, acting on the CH-NH group of donors	3	12
GO:0016649	oxidoreductase activity, acting on the CH-NH group of donors, quinone or similar compound as acceptor	4	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0051536	iron-sulfur cluster binding	3	12
GO:0051539	4 iron, 4 sulfur cluster binding	4	12
GO:0051540	metal cluster binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	452	456	PF00656	0.478
CLV_C14_Caspase3-7	52	56	PF00656	0.387
CLV_MEL_PAP_1	219	225	PF00089	0.465
CLV_NRD_NRD_1	189	191	PF00675	0.437
CLV_PCSK_KEX2_1	189	191	PF00082	0.407
CLV_PCSK_KEX2_1	221	223	PF00082	0.492
CLV_PCSK_PC1ET2_1	221	223	PF00082	0.428
CLV_PCSK_SKI1_1	117	121	PF00082	0.400
CLV_PCSK_SKI1_1	322	326	PF00082	0.353
CLV_PCSK_SKI1_1	425	429	PF00082	0.399
CLV_PCSK_SKI1_1	460	464	PF00082	0.402
DEG_Nend_Nbox_1	1	3	PF02207	0.502
DEG_SCF_FBW7_1	101	106	PF00400	0.475
DOC_ANK_TNKS_1	328	335	PF00023	0.285
DOC_CDC14_PxL_1	336	344	PF14671	0.292
DOC_MAPK_gen_1	460	468	PF00069	0.460
DOC_MAPK_gen_1	469	479	PF00069	0.463
DOC_MAPK_gen_1	503	513	PF00069	0.369
DOC_PP1_RVXF_1	339	345	PF00149	0.328
DOC_PP2B_LxvP_1	357	360	PF13499	0.295
DOC_USP7_MATH_1	103	107	PF00917	0.469
DOC_USP7_MATH_1	115	119	PF00917	0.497
DOC_USP7_MATH_1	16	20	PF00917	0.595
DOC_USP7_MATH_1	368	372	PF00917	0.286
DOC_USP7_UBL2_3	217	221	PF12436	0.331
DOC_WW_Pin1_4	450	455	PF00397	0.480
DOC_WW_Pin1_4	99	104	PF00397	0.485
LIG_14-3-3_CanoR_1	189	196	PF00244	0.549
LIG_14-3-3_CanoR_1	3	9	PF00244	0.528
LIG_14-3-3_CanoR_1	405	409	PF00244	0.344
LIG_14-3-3_CanoR_1	420	424	PF00244	0.388
LIG_APCC_ABBAyCdc20_2	203	209	PF00400	0.288
LIG_BRCT_BRCA1_1	105	109	PF00533	0.356
LIG_CSL_BTD_1	122	125	PF09270	0.529
LIG_eIF4E_1	326	332	PF01652	0.284
LIG_FHA_1	169	175	PF00498	0.472
LIG_FHA_1	214	220	PF00498	0.309
LIG_FHA_1	231	237	PF00498	0.299
LIG_FHA_1	308	314	PF00498	0.326
LIG_FHA_1	390	396	PF00498	0.474
LIG_FHA_1	435	441	PF00498	0.393
LIG_FHA_1	476	482	PF00498	0.252
LIG_FHA_1	553	559	PF00498	0.295
LIG_FHA_2	328	334	PF00498	0.318
LIG_FHA_2	450	456	PF00498	0.509
LIG_FHA_2	559	565	PF00498	0.311
LIG_IBAR_NPY_1	296	298	PF08397	0.311
LIG_Integrin_isoDGR_2	187	189	PF01839	0.377
LIG_LIR_Apic_2	240	245	PF02991	0.322
LIG_LIR_Apic_2	293	297	PF02991	0.272
LIG_LIR_Apic_2	467	473	PF02991	0.372
LIG_LIR_Apic_2	515	519	PF02991	0.223
LIG_LIR_Gen_1	106	115	PF02991	0.500
LIG_LIR_Gen_1	26	33	PF02991	0.308
LIG_LIR_Gen_1	333	344	PF02991	0.326
LIG_LIR_Nem_3	106	112	PF02991	0.389
LIG_LIR_Nem_3	155	160	PF02991	0.313
LIG_LIR_Nem_3	26	31	PF02991	0.315
LIG_LIR_Nem_3	333	339	PF02991	0.308
LIG_LIR_Nem_3	407	413	PF02991	0.349
LIG_LIR_Nem_3	426	432	PF02991	0.484
LIG_LIR_Nem_3	476	482	PF02991	0.273
LIG_LYPXL_S_1	156	160	PF13949	0.348
LIG_Pex14_1	4	8	PF04695	0.614
LIG_Pex14_1	429	433	PF04695	0.450
LIG_Pex14_2	404	408	PF04695	0.301
LIG_Pex14_2	423	427	PF04695	0.332
LIG_SH2_CRK	294	298	PF00017	0.274
LIG_SH2_CRK	433	437	PF00017	0.342
LIG_SH2_GRB2like	294	297	PF00017	0.285
LIG_SH2_NCK_1	294	298	PF00017	0.309
LIG_SH2_NCK_1	380	384	PF00017	0.337
LIG_SH2_SRC	194	197	PF00017	0.362
LIG_SH2_SRC	380	383	PF00017	0.359
LIG_SH2_STAP1	380	384	PF00017	0.337
LIG_SH2_STAP1	400	404	PF00017	0.145
LIG_SH2_STAT3	571	574	PF00017	0.472
LIG_SH2_STAT5	134	137	PF00017	0.344
LIG_SH2_STAT5	288	291	PF00017	0.340
LIG_SH2_STAT5	298	301	PF00017	0.279
LIG_SH2_STAT5	414	417	PF00017	0.468
LIG_SH2_STAT5	433	436	PF00017	0.177
LIG_SH2_STAT5	8	11	PF00017	0.647
LIG_SH3_3	116	122	PF00018	0.447
LIG_SH3_3	255	261	PF00018	0.450
MOD_CK1_1	38	44	PF00069	0.395
MOD_CK1_1	96	102	PF00069	0.484
MOD_CK2_1	327	333	PF00069	0.299
MOD_CK2_1	390	396	PF00069	0.472
MOD_CK2_1	558	564	PF00069	0.311
MOD_Cter_Amidation	187	190	PF01082	0.453
MOD_GlcNHglycan	11	14	PF01048	0.606
MOD_GlcNHglycan	336	339	PF01048	0.414
MOD_GlcNHglycan	37	40	PF01048	0.290
MOD_GlcNHglycan	370	373	PF01048	0.283
MOD_GlcNHglycan	392	395	PF01048	0.464
MOD_GlcNHglycan	487	490	PF01048	0.267
MOD_GlcNHglycan	73	76	PF01048	0.251
MOD_GSK3_1	168	175	PF00069	0.503
MOD_GSK3_1	250	257	PF00069	0.371
MOD_GSK3_1	364	371	PF00069	0.304
MOD_GSK3_1	419	426	PF00069	0.380
MOD_GSK3_1	431	438	PF00069	0.266
MOD_GSK3_1	445	452	PF00069	0.451
MOD_GSK3_1	483	490	PF00069	0.278
MOD_GSK3_1	93	100	PF00069	0.414
MOD_LATS_1	485	491	PF00433	0.287
MOD_N-GLC_1	455	460	PF02516	0.449
MOD_N-GLC_1	483	488	PF02516	0.269
MOD_N-GLC_1	558	563	PF02516	0.328
MOD_NEK2_1	2	7	PF00069	0.514
MOD_NEK2_1	292	297	PF00069	0.294
MOD_NEK2_1	404	409	PF00069	0.295
MOD_NEK2_1	423	428	PF00069	0.426
MOD_NEK2_1	431	436	PF00069	0.354
MOD_NEK2_1	449	454	PF00069	0.522
MOD_NEK2_1	502	507	PF00069	0.435
MOD_NEK2_1	9	14	PF00069	0.516
MOD_NEK2_2	115	120	PF00069	0.500
MOD_NEK2_2	202	207	PF00069	0.287
MOD_PIKK_1	552	558	PF00454	0.287
MOD_PKA_2	188	194	PF00069	0.504
MOD_PKA_2	2	8	PF00069	0.514
MOD_PKA_2	211	217	PF00069	0.298
MOD_PKA_2	404	410	PF00069	0.330
MOD_PKA_2	419	425	PF00069	0.381
MOD_PKA_2	53	59	PF00069	0.372
MOD_Plk_1	267	273	PF00069	0.360
MOD_Plk_1	455	461	PF00069	0.448
MOD_Plk_1	558	564	PF00069	0.343
MOD_Plk_2-3	396	402	PF00069	0.329
MOD_Plk_4	202	208	PF00069	0.286
MOD_Plk_4	250	256	PF00069	0.394
MOD_Plk_4	404	410	PF00069	0.294
MOD_Plk_4	435	441	PF00069	0.367
MOD_Plk_4	93	99	PF00069	0.478
MOD_ProDKin_1	450	456	PF00069	0.474
MOD_ProDKin_1	99	105	PF00069	0.489
MOD_SUMO_rev_2	390	399	PF00179	0.423
MOD_SUMO_rev_2	464	471	PF00179	0.397
TRG_ENDOCYTIC_2	157	160	PF00928	0.334
TRG_ENDOCYTIC_2	302	305	PF00928	0.282
TRG_ENDOCYTIC_2	410	413	PF00928	0.398
TRG_ENDOCYTIC_2	433	436	PF00928	0.321
TRG_ENDOCYTIC_2	529	532	PF00928	0.267
TRG_Pf-PMV_PEXEL_1	143	147	PF00026	0.345
TRG_Pf-PMV_PEXEL_1	329	333	PF00026	0.284

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HU17	Leptomonas seymouri	81%	98%
A0A0S4J6T4	Bodo saltans	68%	100%
A0A1X0NPE8	Trypanosomatidae	71%	100%
A0A422N647	Trypanosoma rangeli	72%	100%
A4H510	Leishmania braziliensis	93%	100%
A4HT84	Leishmania infantum	100%	100%
C9ZUP0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	67%	100%
E9AL72	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
O22854	Arabidopsis thaliana	50%	91%
P09820	Rhizobium meliloti (strain 1021)	24%	100%
P10331	Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110)	24%	100%
P26484	Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / LMG 6465 / NBRC 14845 / NCIMB 13405 / ORS 571)	25%	100%
P53572	Azotobacter vinelandii	23%	100%
P55931	Sus scrofa	54%	93%
P87111	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	48%	91%
P94132	Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)	48%	100%
Q08822	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	49%	91%
Q11190	Caenorhabditis elegans	53%	96%
Q16134	Homo sapiens	54%	93%
Q2KIG0	Bos taurus	54%	93%
Q337B8	Oryza sativa subsp. japonica	50%	100%
Q4QIN2	Leishmania major	98%	100%
Q53208	Sinorhizobium fredii (strain NBRC 101917 / NGR234)	26%	100%
Q54XM6	Dictyostelium discoideum	51%	95%
Q5RDD3	Pongo abelii	54%	93%
Q6UPE1	Rattus norvegicus	54%	93%
Q921G7	Mus musculus	54%	93%
Q9HZP5	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	50%	100%
V5BTC8	Trypanosoma cruzi	71%	100%