LeishMANIAdb
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Bifunctional dihydrofolate reductase-thymidylate synthase

Quick info Annotations Function or PPIs Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:
Bifunctional dihydrofolate reductase-thymidylate synthase
Gene product:
dihydrofolate reductase-thymidylate synthase
Species:
Leishmania donovani
UniProt:
A0A3S5H5T0_LEIDO
TriTrypDb:
LdBPK_060890.1 * , LdCL_060014000 , LDHU3_06.1030
Length:
520

Annotations

Annotations by Jardim et al.

Amino acid metabolism, Dihydrofolate reductase-thymidylate synthase DHFR-TS

Localization

Secreted promastigote
Source Evidence on protein Close homologs
Cuervo et al. no yes: 0
Hassani et al. no yes: 0
Forrest at al. (metacyclic) no yes: 0
Forrest at al. (procyclic) no yes: 0
Silverman et al. no yes: 0
Pissara et al. no yes: 0
Secreted amastigote
Source Evidence on protein Close homologs
Pires et al. no yes: 0
Exosome
Source Evidence on protein Close homologs
Silverman et al. no yes: 0
Glycosome
Source Evidence on protein Close homologs
Jamdhade et al. yes yes: 18
Predictions
Source Evidence on protein Close homologs
DeepLoc
SignalP6 no yes: 0, no: 5
NetGPI no yes: 0, no: 5
Cellular components
Term Name Level Count
GO:0005737 cytoplasm 2 1
GO:0005739 mitochondrion 5 1
GO:0005829 cytosol 2 1
GO:0043226 organelle 2 1
GO:0043227 membrane-bounded organelle 3 1
GO:0043229 intracellular organelle 3 1
GO:0043231 intracellular membrane-bounded organelle 4 1
GO:0110165 cellular anatomical entity 1 1

Expansion

Sequence features

A0A3S5H5T0
Sequence
MSA
Disorder
Secondary
Topology
Domains
SignalP
GPI
Phosphorylations
ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S5H5T0

Function

Biological processes
Term Name Level Count
GO:0006139 nucleobase-containing compound metabolic process 3 6
GO:0006220 pyrimidine nucleotide metabolic process 5 6
GO:0006221 pyrimidine nucleotide biosynthetic process 6 6
GO:0006231 dTMP biosynthetic process 8 6
GO:0006575 cellular modified amino acid metabolic process 3 6
GO:0006725 cellular aromatic compound metabolic process 3 6
GO:0006730 one-carbon metabolic process 3 6
GO:0006753 nucleoside phosphate metabolic process 4 6
GO:0006760 folic acid-containing compound metabolic process 4 6
GO:0006793 phosphorus metabolic process 3 6
GO:0006796 phosphate-containing compound metabolic process 4 6
GO:0006807 nitrogen compound metabolic process 2 6
GO:0008152 metabolic process 1 6
GO:0009058 biosynthetic process 2 6
GO:0009117 nucleotide metabolic process 5 6
GO:0009123 nucleoside monophosphate metabolic process 5 6
GO:0009124 nucleoside monophosphate biosynthetic process 6 6
GO:0009129 pyrimidine nucleoside monophosphate metabolic process 6 6
GO:0009130 pyrimidine nucleoside monophosphate biosynthetic process 7 6
GO:0009157 deoxyribonucleoside monophosphate biosynthetic process 7 6
GO:0009162 deoxyribonucleoside monophosphate metabolic process 6 6
GO:0009165 nucleotide biosynthetic process 6 6
GO:0009176 pyrimidine deoxyribonucleoside monophosphate metabolic process 7 6
GO:0009177 pyrimidine deoxyribonucleoside monophosphate biosynthetic process 8 6
GO:0009219 pyrimidine deoxyribonucleotide metabolic process 6 6
GO:0009221 pyrimidine deoxyribonucleotide biosynthetic process 7 6
GO:0009262 deoxyribonucleotide metabolic process 4 6
GO:0009263 deoxyribonucleotide biosynthetic process 5 6
GO:0009265 2'-deoxyribonucleotide biosynthetic process 6 6
GO:0009394 2'-deoxyribonucleotide metabolic process 5 6
GO:0009396 folic acid-containing compound biosynthetic process 5 6
GO:0009987 cellular process 1 6
GO:0018130 heterocycle biosynthetic process 4 6
GO:0019438 aromatic compound biosynthetic process 4 6
GO:0019637 organophosphate metabolic process 3 6
GO:0019692 deoxyribose phosphate metabolic process 4 6
GO:0032259 methylation 2 6
GO:0034641 cellular nitrogen compound metabolic process 3 6
GO:0034654 nucleobase-containing compound biosynthetic process 4 6
GO:0042398 cellular modified amino acid biosynthetic process 4 6
GO:0042558 pteridine-containing compound metabolic process 4 6
GO:0042559 pteridine-containing compound biosynthetic process 5 6
GO:0044237 cellular metabolic process 2 6
GO:0044238 primary metabolic process 2 6
GO:0044249 cellular biosynthetic process 3 6
GO:0044271 cellular nitrogen compound biosynthetic process 4 6
GO:0044281 small molecule metabolic process 2 6
GO:0046073 dTMP metabolic process 7 6
GO:0046385 deoxyribose phosphate biosynthetic process 5 6
GO:0046483 heterocycle metabolic process 3 6
GO:0046653 tetrahydrofolate metabolic process 5 6
GO:0046654 tetrahydrofolate biosynthetic process 6 6
GO:0055086 nucleobase-containing small molecule metabolic process 3 6
GO:0071704 organic substance metabolic process 2 6
GO:0072527 pyrimidine-containing compound metabolic process 4 6
GO:0072528 pyrimidine-containing compound biosynthetic process 5 6
GO:0090407 organophosphate biosynthetic process 4 6
GO:1901135 carbohydrate derivative metabolic process 3 6
GO:1901137 carbohydrate derivative biosynthetic process 4 6
GO:1901293 nucleoside phosphate biosynthetic process 5 6
GO:1901360 organic cyclic compound metabolic process 3 6
GO:1901362 organic cyclic compound biosynthetic process 4 6
GO:1901564 organonitrogen compound metabolic process 3 6
GO:1901566 organonitrogen compound biosynthetic process 4 6
GO:1901576 organic substance biosynthetic process 3 6
Molecular functions
Term Name Level Count
GO:0003824 catalytic activity 1 6
GO:0004146 dihydrofolate reductase activity 5 6
GO:0004799 thymidylate synthase activity 6 6
GO:0008168 methyltransferase activity 4 6
GO:0016491 oxidoreductase activity 2 6
GO:0016645 oxidoreductase activity, acting on the CH-NH group of donors 3 6
GO:0016646 oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor 4 6
GO:0016740 transferase activity 2 6
GO:0016741 transferase activity, transferring one-carbon groups 3 6
GO:0042083 5,10-methylenetetrahydrofolate-dependent methyltransferase activity 5 6

Putative motif mimicry

Leishmania From To Domain/Motif Score
CLV_C14_Caspase3-7 215 219 PF00656 0.435
CLV_C14_Caspase3-7 270 274 PF00656 0.506
CLV_C14_Caspase3-7 321 325 PF00656 0.506
CLV_C14_Caspase3-7 458 462 PF00656 0.506
CLV_NRD_NRD_1 212 214 PF00675 0.285
CLV_NRD_NRD_1 380 382 PF00675 0.306
CLV_NRD_NRD_1 80 82 PF00675 0.270
CLV_PCSK_KEX2_1 119 121 PF00082 0.270
CLV_PCSK_KEX2_1 212 214 PF00082 0.270
CLV_PCSK_KEX2_1 282 284 PF00082 0.344
CLV_PCSK_KEX2_1 380 382 PF00082 0.306
CLV_PCSK_KEX2_1 73 75 PF00082 0.306
CLV_PCSK_PC1ET2_1 119 121 PF00082 0.270
CLV_PCSK_PC1ET2_1 282 284 PF00082 0.306
CLV_PCSK_PC1ET2_1 73 75 PF00082 0.306
CLV_PCSK_SKI1_1 243 247 PF00082 0.306
CLV_PCSK_SKI1_1 282 286 PF00082 0.306
CLV_PCSK_SKI1_1 320 324 PF00082 0.306
CLV_PCSK_SKI1_1 326 330 PF00082 0.213
CLV_PCSK_SKI1_1 380 384 PF00082 0.306
CLV_PCSK_SKI1_1 394 398 PF00082 0.206
CLV_PCSK_SKI1_1 492 496 PF00082 0.270
CLV_PCSK_SKI1_1 92 96 PF00082 0.306
DEG_APCC_DBOX_1 234 242 PF00400 0.363
DOC_CYCLIN_yCln2_LP_2 428 434 PF00134 0.506
DOC_MAPK_FxFP_2 19 22 PF00069 0.407
DOC_MAPK_FxFP_2 193 196 PF00069 0.470
DOC_MAPK_gen_1 81 89 PF00069 0.486
DOC_MAPK_gen_1 92 102 PF00069 0.447
DOC_MAPK_MEF2A_6 25 33 PF00069 0.363
DOC_MAPK_MEF2A_6 422 430 PF00069 0.506
DOC_MAPK_MEF2A_6 82 91 PF00069 0.483
DOC_MAPK_MEF2A_6 95 102 PF00069 0.436
DOC_PP1_RVXF_1 280 287 PF00149 0.506
DOC_PP1_RVXF_1 378 385 PF00149 0.435
DOC_PP2B_LxvP_1 111 114 PF13499 0.470
DOC_PP2B_LxvP_1 428 431 PF13499 0.506
DOC_PP4_FxxP_1 19 22 PF00568 0.297
DOC_PP4_FxxP_1 193 196 PF00568 0.470
DOC_PP4_FxxP_1 8 11 PF00568 0.435
DOC_SPAK_OSR1_1 7 11 PF12202 0.425
DOC_USP7_MATH_1 145 149 PF00917 0.520
DOC_USP7_MATH_1 20 24 PF00917 0.502
DOC_USP7_MATH_1 471 475 PF00917 0.417
DOC_WW_Pin1_4 165 170 PF00397 0.488
LIG_14-3-3_CanoR_1 181 187 PF00244 0.510
LIG_14-3-3_CanoR_1 212 220 PF00244 0.517
LIG_14-3-3_CanoR_1 268 272 PF00244 0.506
LIG_14-3-3_CanoR_1 326 334 PF00244 0.507
LIG_14-3-3_CanoR_1 381 389 PF00244 0.506
LIG_14-3-3_CanoR_1 81 85 PF00244 0.506
LIG_Actin_WH2_2 435 453 PF00022 0.506
LIG_BIR_II_1 1 5 PF00653 0.492
LIG_BRCT_BRCA1_1 318 322 PF00533 0.506
LIG_CtBP_PxDLS_1 50 54 PF00389 0.506
LIG_EVH1_1 193 197 PF00568 0.506
LIG_FHA_1 245 251 PF00498 0.459
LIG_FHA_1 257 263 PF00498 0.412
LIG_FHA_1 279 285 PF00498 0.506
LIG_FHA_1 410 416 PF00498 0.453
LIG_FHA_1 439 445 PF00498 0.470
LIG_FHA_1 58 64 PF00498 0.506
LIG_FHA_2 105 111 PF00498 0.506
LIG_FHA_2 13 19 PF00498 0.405
LIG_FHA_2 200 206 PF00498 0.476
LIG_FHA_2 456 462 PF00498 0.506
LIG_FHA_2 499 505 PF00498 0.453
LIG_LIR_Apic_2 18 22 PF02991 0.418
LIG_LIR_Apic_2 190 196 PF02991 0.506
LIG_LIR_Apic_2 44 50 PF02991 0.506
LIG_LIR_Gen_1 234 245 PF02991 0.408
LIG_LIR_Gen_1 436 445 PF02991 0.506
LIG_LIR_Gen_1 83 91 PF02991 0.506
LIG_LIR_Nem_3 18 24 PF02991 0.418
LIG_LIR_Nem_3 190 195 PF02991 0.506
LIG_LIR_Nem_3 234 240 PF02991 0.408
LIG_LIR_Nem_3 350 354 PF02991 0.470
LIG_LIR_Nem_3 436 440 PF02991 0.506
LIG_LIR_Nem_3 83 87 PF02991 0.506
LIG_REV1ctd_RIR_1 53 61 PF16727 0.506
LIG_SH2_CRK 437 441 PF00017 0.506
LIG_SH2_GRB2like 407 410 PF00017 0.506
LIG_SH2_NCK_1 183 187 PF00017 0.488
LIG_SH2_SRC 162 165 PF00017 0.470
LIG_SH2_SRC 508 511 PF00017 0.470
LIG_SH2_STAP1 162 166 PF00017 0.506
LIG_SH2_STAT3 340 343 PF00017 0.506
LIG_SH2_STAT5 146 149 PF00017 0.492
LIG_SH2_STAT5 154 157 PF00017 0.463
LIG_SH2_STAT5 178 181 PF00017 0.506
LIG_SH2_STAT5 183 186 PF00017 0.470
LIG_SH2_STAT5 227 230 PF00017 0.414
LIG_SH2_STAT5 237 240 PF00017 0.435
LIG_SH2_STAT5 407 410 PF00017 0.506
LIG_SH2_STAT5 508 511 PF00017 0.445
LIG_SH3_1 191 197 PF00018 0.514
LIG_SH3_2 510 515 PF14604 0.453
LIG_SH3_3 191 197 PF00018 0.514
LIG_SH3_3 506 512 PF00018 0.504
LIG_SUMO_SIM_anti_2 126 131 PF11976 0.506
LIG_SUMO_SIM_par_1 28 34 PF11976 0.506
LIG_TRAF2_1 107 110 PF00917 0.470
LIG_UBA3_1 111 119 PF00899 0.313
LIG_UBA3_1 240 246 PF00899 0.363
LIG_UBA3_1 439 446 PF00899 0.363
MOD_CK1_1 104 110 PF00069 0.413
MOD_CK1_1 149 155 PF00069 0.426
MOD_CK1_1 413 419 PF00069 0.313
MOD_CK2_1 104 110 PF00069 0.363
MOD_CK2_1 145 151 PF00069 0.468
MOD_CK2_1 365 371 PF00069 0.288
MOD_CK2_1 471 477 PF00069 0.238
MOD_CK2_1 498 504 PF00069 0.338
MOD_Cter_Amidation 117 120 PF01082 0.288
MOD_Cter_Amidation 210 213 PF01082 0.313
MOD_Cter_Amidation 79 82 PF01082 0.313
MOD_GSK3_1 100 107 PF00069 0.363
MOD_GSK3_1 145 152 PF00069 0.422
MOD_GSK3_1 177 184 PF00069 0.271
MOD_GSK3_1 252 259 PF00069 0.320
MOD_GSK3_1 322 329 PF00069 0.363
MOD_GSK3_1 365 372 PF00069 0.288
MOD_GSK3_1 409 416 PF00069 0.288
MOD_N-GLC_1 198 203 PF02516 0.238
MOD_N-GLC_1 316 321 PF02516 0.363
MOD_NEK2_1 206 211 PF00069 0.435
MOD_NEK2_1 267 272 PF00069 0.463
MOD_NEK2_1 278 283 PF00069 0.298
MOD_NEK2_1 322 327 PF00069 0.413
MOD_NEK2_1 382 387 PF00069 0.363
MOD_NEK2_1 455 460 PF00069 0.363
MOD_NEK2_2 258 263 PF00069 0.363
MOD_OFUCOSY 417 424 PF10250 0.363
MOD_PIKK_1 206 212 PF00454 0.363
MOD_PIKK_1 471 477 PF00454 0.238
MOD_PK_1 25 31 PF00069 0.363
MOD_PKA_1 212 218 PF00069 0.313
MOD_PKA_2 212 218 PF00069 0.385
MOD_PKA_2 267 273 PF00069 0.363
MOD_PKA_2 80 86 PF00069 0.363
MOD_Plk_1 413 419 PF00069 0.363
MOD_Plk_1 57 63 PF00069 0.348
MOD_Plk_4 438 444 PF00069 0.363
MOD_Plk_4 482 488 PF00069 0.363
MOD_Plk_4 58 64 PF00069 0.346
MOD_ProDKin_1 165 171 PF00069 0.338
MOD_SUMO_for_1 488 491 PF00179 0.363
MOD_SUMO_for_1 514 517 PF00179 0.338
MOD_SUMO_rev_2 324 334 PF00179 0.288
TRG_DiLeu_BaEn_1 136 141 PF01217 0.338
TRG_DiLeu_BaEn_2 489 495 PF01217 0.263
TRG_DiLeu_BaEn_3 291 297 PF01217 0.363
TRG_DiLeu_BaEn_4 233 239 PF01217 0.363
TRG_DiLeu_BaEn_4 490 496 PF01217 0.313
TRG_DiLeu_BaLyEn_6 274 279 PF01217 0.363
TRG_DiLeu_BaLyEn_6 378 383 PF01217 0.363
TRG_DiLeu_LyEn_5 136 141 PF01217 0.288
TRG_ENDOCYTIC_2 237 240 PF00928 0.363
TRG_ENDOCYTIC_2 351 354 PF00928 0.363
TRG_ENDOCYTIC_2 437 440 PF00928 0.363
TRG_ER_diArg_1 380 382 PF00400 0.363
TRG_Pf-PMV_PEXEL_1 326 330 PF00026 0.438
TRG_Pf-PMV_PEXEL_1 367 371 PF00026 0.238

Homologs

Protein Taxonomy Sequence identity Coverage
A0A0N1IJX9 Leptomonas seymouri 78% 98%
A0A0S4JJI0 Bodo saltans 61% 100%
A0A1X0NKM2 Trypanosomatidae 68% 100%
A0A3R7P0P8 Trypanosoma rangeli 66% 100%
A4H4P8 Leishmania braziliensis 88% 100%
C9ZTK8 Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972) 62% 99%
E9AKW4 Leishmania mexicana (strain MHOM/GT/2001/U1103) 94% 100%
O81395 Zea mays 46% 100%
P07382 Leishmania major 96% 100%
P16126 Leishmania amazonensis 94% 100%
P20712 Plasmodium chabaudi 37% 89%
P45350 Daucus carota 45% 98%
P51820 Glycine max 45% 98%
Q05762 Arabidopsis thaliana 46% 100%
Q05763 Arabidopsis thaliana 47% 92%
Q23695 Crithidia fasciculata 82% 100%
Q27713 Plasmodium berghei (strain Anka) 36% 89%
Q27783 Trypanosoma brucei brucei 62% 99%
Q27793 Trypanosoma cruzi 67% 100%
Q27828 Paramecium tetraurelia 43% 100%
Q2QRX6 Oryza sativa subsp. japonica 47% 100%
Q8MQV3 Leishmania infantum 100% 100%
V5DPL1 Trypanosoma cruzi 67% 100%

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LeishMANIAdb - Server version: v0.0.2. - Database version: v0.0.1. - ChangeLog - © 2022-2025 Protein Bioinformatics Research Group, Institute of Enzymology, RCNS