Ubiquitin-conjugating enzyme E2, putative

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-conjugating enzyme E2, putative

Species:

Leishmania donovani

UniProt:

A0A3S5H5K9_LEIDO

TriTrypDb:

LdBPK_050930.1 , LdCL_050014400 , LDHU3_05.1020

Length:

464

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	4
GO:0110165	cellular anatomical entity	1	4
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1

Expansion

Sequence features

A0A3S5H5K9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S5H5K9

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0016567	protein ubiquitination	7	1
GO:0019538	protein metabolic process	3	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044238	primary metabolic process	2	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	3
GO:0004842	ubiquitin-protein transferase activity	4	1
GO:0016740	transferase activity	2	1
GO:0016874	ligase activity	2	3
GO:0019787	ubiquitin-like protein transferase activity	3	1
GO:0061631	ubiquitin conjugating enzyme activity	5	1
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	1
GO:0140096	catalytic activity, acting on a protein	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	208	212	PF00656	0.725
CLV_NRD_NRD_1	16	18	PF00675	0.278
CLV_NRD_NRD_1	455	457	PF00675	0.312
CLV_PCSK_KEX2_1	16	18	PF00082	0.275
CLV_PCSK_KEX2_1	455	457	PF00082	0.312
CLV_PCSK_KEX2_1	9	11	PF00082	0.275
CLV_PCSK_PC1ET2_1	9	11	PF00082	0.275
CLV_PCSK_SKI1_1	300	304	PF00082	0.487
CLV_PCSK_SKI1_1	421	425	PF00082	0.475
DEG_APCC_DBOX_1	454	462	PF00400	0.388
DEG_SCF_FBW7_2	363	370	PF00400	0.651
DOC_CKS1_1	290	295	PF01111	0.727
DOC_MAPK_gen_1	331	340	PF00069	0.597
DOC_MAPK_RevD_3	442	456	PF00069	0.385
DOC_PP1_RVXF_1	419	425	PF00149	0.640
DOC_PP2B_LxvP_1	187	190	PF13499	0.688
DOC_PP2B_LxvP_1	73	76	PF13499	0.475
DOC_SPAK_OSR1_1	86	90	PF12202	0.529
DOC_USP7_MATH_1	195	199	PF00917	0.710
DOC_USP7_MATH_1	225	229	PF00917	0.713
DOC_USP7_MATH_1	238	242	PF00917	0.753
DOC_USP7_MATH_1	268	272	PF00917	0.727
DOC_USP7_MATH_1	384	388	PF00917	0.692
DOC_USP7_MATH_1	399	403	PF00917	0.680
DOC_WW_Pin1_4	174	179	PF00397	0.652
DOC_WW_Pin1_4	215	220	PF00397	0.762
DOC_WW_Pin1_4	223	228	PF00397	0.695
DOC_WW_Pin1_4	229	234	PF00397	0.684
DOC_WW_Pin1_4	260	265	PF00397	0.723
DOC_WW_Pin1_4	278	283	PF00397	0.745
DOC_WW_Pin1_4	289	294	PF00397	0.687
DOC_WW_Pin1_4	325	330	PF00397	0.747
DOC_WW_Pin1_4	363	368	PF00397	0.644
DOC_WW_Pin1_4	379	384	PF00397	0.666
DOC_WW_Pin1_4	401	406	PF00397	0.631
LIG_Actin_WH2_2	442	457	PF00022	0.380
LIG_APCC_ABBA_1	170	175	PF00400	0.576
LIG_BIR_II_1	1	5	PF00653	0.561
LIG_BIR_III_2	380	384	PF00653	0.684
LIG_BRCT_BRCA1_1	265	269	PF00533	0.669
LIG_BRCT_BRCA1_1	444	448	PF00533	0.286
LIG_BRCT_BRCA1_1	83	87	PF00533	0.529
LIG_deltaCOP1_diTrp_1	144	153	PF00928	0.585
LIG_deltaCOP1_diTrp_1	39	42	PF00928	0.475
LIG_EH1_1	447	455	PF00400	0.279
LIG_EVH1_1	187	191	PF00568	0.770
LIG_FHA_1	106	112	PF00498	0.475
LIG_FHA_1	140	146	PF00498	0.570
LIG_FHA_1	160	166	PF00498	0.586
LIG_FHA_1	281	287	PF00498	0.760
LIG_FHA_1	380	386	PF00498	0.728
LIG_FHA_2	174	180	PF00498	0.614
LIG_GBD_Chelix_1	443	451	PF00786	0.331
LIG_LIR_Apic_2	27	32	PF02991	0.529
LIG_LIR_Gen_1	322	332	PF02991	0.719
LIG_LIR_Gen_1	445	454	PF02991	0.279
LIG_LIR_Nem_3	12	18	PF02991	0.475
LIG_LIR_Nem_3	322	327	PF02991	0.694
LIG_LIR_Nem_3	445	451	PF02991	0.286
LIG_MYND_1	185	189	PF01753	0.646
LIG_NRBOX	457	463	PF00104	0.431
LIG_Pex14_1	105	109	PF04695	0.475
LIG_Pex14_2	38	42	PF04695	0.475
LIG_REV1ctd_RIR_1	78	87	PF16727	0.475
LIG_SH2_CRK	15	19	PF00017	0.460
LIG_SH2_PTP2	29	32	PF00017	0.495
LIG_SH2_SRC	109	112	PF00017	0.547
LIG_SH2_STAP1	109	113	PF00017	0.475
LIG_SH2_STAP1	3	7	PF00017	0.526
LIG_SH2_STAP1	355	359	PF00017	0.619
LIG_SH2_STAT5	29	32	PF00017	0.495
LIG_SH3_1	186	192	PF00018	0.668
LIG_SH3_1	261	267	PF00018	0.704
LIG_SH3_1	69	75	PF00018	0.475
LIG_SH3_3	182	188	PF00018	0.713
LIG_SH3_3	189	195	PF00018	0.718
LIG_SH3_3	216	222	PF00018	0.724
LIG_SH3_3	248	254	PF00018	0.704
LIG_SH3_3	261	267	PF00018	0.672
LIG_SH3_3	276	282	PF00018	0.651
LIG_SH3_3	330	336	PF00018	0.686
LIG_SH3_3	337	343	PF00018	0.709
LIG_SH3_3	410	416	PF00018	0.666
LIG_SH3_3	69	75	PF00018	0.475
LIG_SUMO_SIM_par_1	136	142	PF11976	0.559
LIG_TRAF2_1	32	35	PF00917	0.426
LIG_TRFH_1	71	75	PF08558	0.312
LIG_UBA3_1	137	143	PF00899	0.366
LIG_WRC_WIRS_1	443	448	PF05994	0.340
MOD_CDC14_SPxK_1	177	180	PF00782	0.569
MOD_CDC14_SPxK_1	328	331	PF00782	0.652
MOD_CDK_SPK_2	363	368	PF00069	0.487
MOD_CDK_SPxK_1	174	180	PF00069	0.556
MOD_CDK_SPxK_1	325	331	PF00069	0.660
MOD_CK1_1	2	8	PF00069	0.422
MOD_CK1_1	226	232	PF00069	0.687
MOD_CK1_1	304	310	PF00069	0.677
MOD_CK1_1	322	328	PF00069	0.489
MOD_CK1_1	404	410	PF00069	0.557
MOD_CK1_1	95	101	PF00069	0.330
MOD_CK2_1	173	179	PF00069	0.401
MOD_CK2_1	304	310	PF00069	0.703
MOD_GlcNHglycan	197	200	PF01048	0.651
MOD_GlcNHglycan	223	226	PF01048	0.716
MOD_GlcNHglycan	303	306	PF01048	0.765
MOD_GlcNHglycan	386	389	PF01048	0.609
MOD_GlcNHglycan	401	404	PF01048	0.455
MOD_GlcNHglycan	5	8	PF01048	0.391
MOD_GlcNHglycan	83	86	PF01048	0.452
MOD_GSK3_1	147	154	PF00069	0.389
MOD_GSK3_1	221	228	PF00069	0.696
MOD_GSK3_1	274	281	PF00069	0.612
MOD_GSK3_1	379	386	PF00069	0.618
MOD_GSK3_1	401	408	PF00069	0.505
MOD_GSK3_1	92	99	PF00069	0.324
MOD_NEK2_1	113	118	PF00069	0.312
MOD_NEK2_1	153	158	PF00069	0.476
MOD_NEK2_1	269	274	PF00069	0.620
MOD_NEK2_1	429	434	PF00069	0.678
MOD_NEK2_1	65	70	PF00069	0.328
MOD_NEK2_1	79	84	PF00069	0.371
MOD_NEK2_2	40	45	PF00069	0.312
MOD_PIKK_1	165	171	PF00454	0.422
MOD_PKA_1	9	15	PF00069	0.312
MOD_PKA_2	9	15	PF00069	0.329
MOD_Plk_1	429	435	PF00069	0.599
MOD_Plk_1	50	56	PF00069	0.331
MOD_Plk_4	133	139	PF00069	0.362
MOD_Plk_4	358	364	PF00069	0.545
MOD_Plk_4	442	448	PF00069	0.388
MOD_ProDKin_1	174	180	PF00069	0.562
MOD_ProDKin_1	215	221	PF00069	0.710
MOD_ProDKin_1	223	229	PF00069	0.619
MOD_ProDKin_1	231	237	PF00069	0.580
MOD_ProDKin_1	260	266	PF00069	0.658
MOD_ProDKin_1	278	284	PF00069	0.687
MOD_ProDKin_1	289	295	PF00069	0.606
MOD_ProDKin_1	325	331	PF00069	0.693
MOD_ProDKin_1	363	369	PF00069	0.552
MOD_ProDKin_1	379	385	PF00069	0.579
MOD_ProDKin_1	401	407	PF00069	0.531
MOD_SUMO_for_1	201	204	PF00179	0.641
MOD_SUMO_for_1	308	311	PF00179	0.619
MOD_SUMO_rev_2	136	145	PF00179	0.340
TRG_DiLeu_BaLyEn_6	433	438	PF01217	0.474
TRG_ENDOCYTIC_2	15	18	PF00928	0.312
TRG_ER_diArg_1	15	17	PF00400	0.315
TRG_ER_diArg_1	454	456	PF00400	0.312

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1C5	Leptomonas seymouri	45%	95%
A4H4D6	Leishmania braziliensis	59%	96%
A4HSL5	Leishmania infantum	97%	100%
E9AKJ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	68%	96%
Q4QJA9	Leishmania major	81%	100%