Mitotic spindle assembly checkpoint protein MAD1

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania donovani

UniProt:

A0A3S5H5F6_LEIDO

TriTrypDb:

LdBPK_050050.1 , LdCL_050005400 , LDHU3_05.0080

Length:

833

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005929	cilium	4	1
GO:0042995	cell projection	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0110165	cellular anatomical entity	1	2
GO:0120025	plasma membrane bounded cell projection	3	1

Expansion

Sequence features

A0A3S5H5F6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3S5H5F6

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006163	purine nucleotide metabolic process	5	11
GO:0006164	purine nucleotide biosynthetic process	6	11
GO:0006171	cAMP biosynthetic process	8	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006753	nucleoside phosphate metabolic process	4	11
GO:0006793	phosphorus metabolic process	3	11
GO:0006796	phosphate-containing compound metabolic process	4	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009058	biosynthetic process	2	11
GO:0009117	nucleotide metabolic process	5	11
GO:0009150	purine ribonucleotide metabolic process	6	11
GO:0009152	purine ribonucleotide biosynthetic process	7	11
GO:0009165	nucleotide biosynthetic process	6	11
GO:0009187	cyclic nucleotide metabolic process	6	11
GO:0009190	cyclic nucleotide biosynthetic process	7	11
GO:0009259	ribonucleotide metabolic process	5	11
GO:0009260	ribonucleotide biosynthetic process	6	11
GO:0009987	cellular process	1	11
GO:0018130	heterocycle biosynthetic process	4	11
GO:0019438	aromatic compound biosynthetic process	4	11
GO:0019637	organophosphate metabolic process	3	11
GO:0019693	ribose phosphate metabolic process	4	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0034654	nucleobase-containing compound biosynthetic process	4	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	11
GO:0044271	cellular nitrogen compound biosynthetic process	4	11
GO:0044281	small molecule metabolic process	2	11
GO:0046058	cAMP metabolic process	7	11
GO:0046390	ribose phosphate biosynthetic process	5	11
GO:0046483	heterocycle metabolic process	3	11
GO:0052652	cyclic purine nucleotide metabolic process	6	11
GO:0055086	nucleobase-containing small molecule metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0072521	purine-containing compound metabolic process	4	11
GO:0072522	purine-containing compound biosynthetic process	5	11
GO:0090407	organophosphate biosynthetic process	4	11
GO:1901135	carbohydrate derivative metabolic process	3	11
GO:1901137	carbohydrate derivative biosynthetic process	4	11
GO:1901293	nucleoside phosphate biosynthetic process	5	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901362	organic cyclic compound biosynthetic process	4	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:1901566	organonitrogen compound biosynthetic process	4	11
GO:1901576	organic substance biosynthetic process	3	11
GO:0007165	signal transduction	2	1
GO:0035556	intracellular signal transduction	3	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0065007	biological regulation	1	1

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	528	532	PF00656	0.427
CLV_C14_Caspase3-7	81	85	PF00656	0.630
CLV_NRD_NRD_1	112	114	PF00675	0.626
CLV_NRD_NRD_1	367	369	PF00675	0.745
CLV_NRD_NRD_1	425	427	PF00675	0.613
CLV_NRD_NRD_1	600	602	PF00675	0.517
CLV_NRD_NRD_1	791	793	PF00675	0.645
CLV_NRD_NRD_1	823	825	PF00675	0.500
CLV_PCSK_KEX2_1	112	114	PF00082	0.608
CLV_PCSK_KEX2_1	266	268	PF00082	0.635
CLV_PCSK_KEX2_1	367	369	PF00082	0.651
CLV_PCSK_KEX2_1	425	427	PF00082	0.613
CLV_PCSK_KEX2_1	600	602	PF00082	0.553
CLV_PCSK_KEX2_1	791	793	PF00082	0.648
CLV_PCSK_PC1ET2_1	266	268	PF00082	0.635
CLV_PCSK_SKI1_1	151	155	PF00082	0.514
CLV_PCSK_SKI1_1	204	208	PF00082	0.631
CLV_PCSK_SKI1_1	24	28	PF00082	0.605
CLV_PCSK_SKI1_1	330	334	PF00082	0.595
CLV_PCSK_SKI1_1	353	357	PF00082	0.578
CLV_PCSK_SKI1_1	368	372	PF00082	0.699
CLV_PCSK_SKI1_1	407	411	PF00082	0.638
CLV_PCSK_SKI1_1	646	650	PF00082	0.530
CLV_PCSK_SKI1_1	710	714	PF00082	0.534
CLV_Separin_Metazoa	457	461	PF03568	0.568
CLV_Separin_Metazoa	66	70	PF03568	0.561
DEG_Nend_UBRbox_3	1	3	PF02207	0.790
DEG_SPOP_SBC_1	216	220	PF00917	0.423
DOC_CDC14_PxL_1	305	313	PF14671	0.600
DOC_CDC14_PxL_1	320	328	PF14671	0.575
DOC_CYCLIN_yClb5_NLxxxL_5	415	424	PF00134	0.659
DOC_CYCLIN_yCln2_LP_2	718	721	PF00134	0.559
DOC_MAPK_gen_1	266	274	PF00069	0.606
DOC_MAPK_gen_1	50	59	PF00069	0.652
DOC_MAPK_gen_1	538	546	PF00069	0.374
DOC_MAPK_gen_1	681	690	PF00069	0.559
DOC_MAPK_MEF2A_6	267	276	PF00069	0.582
DOC_MAPK_MEF2A_6	436	445	PF00069	0.431
DOC_PP1_RVXF_1	175	182	PF00149	0.580
DOC_PP1_RVXF_1	410	416	PF00149	0.534
DOC_PP1_RVXF_1	434	441	PF00149	0.446
DOC_PP1_RVXF_1	447	453	PF00149	0.410
DOC_PP2B_LxvP_1	326	329	PF13499	0.517
DOC_PP2B_LxvP_1	443	446	PF13499	0.481
DOC_PP2B_LxvP_1	520	523	PF13499	0.549
DOC_PP2B_LxvP_1	718	721	PF13499	0.536
DOC_PP4_FxxP_1	36	39	PF00568	0.585
DOC_PP4_FxxP_1	648	651	PF00568	0.600
DOC_USP7_MATH_1	127	131	PF00917	0.563
DOC_USP7_MATH_1	216	220	PF00917	0.598
DOC_USP7_MATH_1	44	48	PF00917	0.655
DOC_USP7_MATH_1	514	518	PF00917	0.451
DOC_USP7_MATH_1	617	621	PF00917	0.465
DOC_USP7_MATH_1	658	662	PF00917	0.605
DOC_USP7_UBL2_3	162	166	PF12436	0.627
DOC_WW_Pin1_4	1	6	PF00397	0.663
DOC_WW_Pin1_4	646	651	PF00397	0.559
LIG_14-3-3_CanoR_1	151	160	PF00244	0.552
LIG_14-3-3_CanoR_1	232	237	PF00244	0.538
LIG_14-3-3_CanoR_1	267	275	PF00244	0.550
LIG_14-3-3_CanoR_1	407	416	PF00244	0.572
LIG_14-3-3_CanoR_1	460	464	PF00244	0.461
LIG_14-3-3_CanoR_1	501	510	PF00244	0.427
LIG_14-3-3_CanoR_1	541	545	PF00244	0.410
LIG_14-3-3_CanoR_1	6	13	PF00244	0.777
LIG_14-3-3_CanoR_1	791	795	PF00244	0.680
LIG_Actin_WH2_2	183	200	PF00022	0.584
LIG_Actin_WH2_2	459	475	PF00022	0.522
LIG_BRCT_BRCA1_1	142	146	PF00533	0.463
LIG_deltaCOP1_diTrp_1	508	515	PF00928	0.448
LIG_FHA_1	192	198	PF00498	0.518
LIG_FHA_1	25	31	PF00498	0.479
LIG_FHA_1	267	273	PF00498	0.531
LIG_FHA_1	408	414	PF00498	0.650
LIG_FHA_1	467	473	PF00498	0.557
LIG_FHA_1	54	60	PF00498	0.603
LIG_FHA_1	613	619	PF00498	0.519
LIG_FHA_1	7	13	PF00498	0.705
LIG_FHA_1	785	791	PF00498	0.645
LIG_FHA_1	795	801	PF00498	0.601
LIG_FHA_1	807	813	PF00498	0.531
LIG_FHA_2	783	789	PF00498	0.582
LIG_FHA_2	79	85	PF00498	0.552
LIG_Integrin_isoDGR_2	447	449	PF01839	0.470
LIG_LIR_Apic_2	33	39	PF02991	0.584
LIG_LIR_Apic_2	550	555	PF02991	0.453
LIG_LIR_Apic_2	645	651	PF02991	0.568
LIG_LIR_Gen_1	298	309	PF02991	0.456
LIG_LIR_Gen_1	437	446	PF02991	0.433
LIG_LIR_Gen_1	730	740	PF02991	0.559
LIG_LIR_Gen_1	743	753	PF02991	0.551
LIG_LIR_Nem_3	208	214	PF02991	0.635
LIG_LIR_Nem_3	298	304	PF02991	0.445
LIG_LIR_Nem_3	386	392	PF02991	0.540
LIG_LIR_Nem_3	437	443	PF02991	0.434
LIG_LIR_Nem_3	476	480	PF02991	0.406
LIG_LIR_Nem_3	743	749	PF02991	0.577
LIG_LIR_Nem_3	84	90	PF02991	0.523
LIG_LYPXL_SIV_4	346	354	PF13949	0.547
LIG_PCNA_yPIPBox_3	600	609	PF02747	0.454
LIG_PCNA_yPIPBox_3	699	710	PF02747	0.574
LIG_Pex14_2	300	304	PF04695	0.454
LIG_PTB_Apo_2	563	570	PF02174	0.569
LIG_PTB_Phospho_1	563	569	PF10480	0.577
LIG_SH2_CRK	467	471	PF00017	0.516
LIG_SH2_CRK	537	541	PF00017	0.457
LIG_SH2_PTP2	552	555	PF00017	0.561
LIG_SH2_SRC	552	555	PF00017	0.561
LIG_SH2_STAP1	247	251	PF00017	0.618
LIG_SH2_STAP1	483	487	PF00017	0.516
LIG_SH2_STAT3	247	250	PF00017	0.615
LIG_SH2_STAT3	769	772	PF00017	0.535
LIG_SH2_STAT5	22	25	PF00017	0.613
LIG_SH2_STAT5	292	295	PF00017	0.450
LIG_SH2_STAT5	414	417	PF00017	0.589
LIG_SH2_STAT5	552	555	PF00017	0.556
LIG_SH2_STAT5	746	749	PF00017	0.601
LIG_SH2_STAT5	769	772	PF00017	0.503
LIG_SH3_3	427	433	PF00018	0.502
LIG_SH3_3	652	658	PF00018	0.646
LIG_SUMO_SIM_anti_2	268	276	PF11976	0.643
LIG_SUMO_SIM_anti_2	686	694	PF11976	0.578
LIG_SUMO_SIM_par_1	272	278	PF11976	0.558
LIG_SUMO_SIM_par_1	55	61	PF11976	0.583
LIG_SUMO_SIM_par_1	639	645	PF11976	0.475
LIG_TRAF2_1	206	209	PF00917	0.490
LIG_TRAF2_1	525	528	PF00917	0.516
LIG_TRAF2_1	755	758	PF00917	0.591
LIG_TRFH_1	716	720	PF08558	0.530
LIG_TYR_ITIM	465	470	PF00017	0.507
LIG_TYR_ITIM	535	540	PF00017	0.463
LIG_WRC_WIRS_1	297	302	PF05994	0.566
LIG_WRC_WIRS_1	618	623	PF05994	0.482
MOD_CDK_SPK_2	1	6	PF00069	0.658
MOD_CDK_SPxxK_3	1	8	PF00069	0.661
MOD_CDK_SPxxK_3	646	653	PF00069	0.500
MOD_CK1_1	218	224	PF00069	0.618
MOD_CK1_1	53	59	PF00069	0.658
MOD_CK1_1	612	618	PF00069	0.536
MOD_CK1_1	620	626	PF00069	0.584
MOD_CK1_1	660	666	PF00069	0.472
MOD_CK1_1	697	703	PF00069	0.589
MOD_CK2_1	134	140	PF00069	0.497
MOD_CK2_1	196	202	PF00069	0.574
MOD_CK2_1	390	396	PF00069	0.570
MOD_CK2_1	397	403	PF00069	0.593
MOD_CK2_1	730	736	PF00069	0.547
MOD_CK2_1	782	788	PF00069	0.563
MOD_CK2_1	88	94	PF00069	0.567
MOD_GlcNHglycan	46	49	PF01048	0.622
MOD_GlcNHglycan	516	519	PF01048	0.451
MOD_GlcNHglycan	52	55	PF01048	0.576
MOD_GlcNHglycan	554	558	PF01048	0.542
MOD_GlcNHglycan	624	627	PF01048	0.644
MOD_GlcNHglycan	774	778	PF01048	0.612
MOD_GSK3_1	192	199	PF00069	0.549
MOD_GSK3_1	288	295	PF00069	0.539
MOD_GSK3_1	296	303	PF00069	0.477
MOD_GSK3_1	481	488	PF00069	0.385
MOD_GSK3_1	69	76	PF00069	0.506
MOD_GSK3_1	694	701	PF00069	0.578
MOD_GSK3_1	719	726	PF00069	0.524
MOD_GSK3_1	78	85	PF00069	0.490
MOD_GSK3_1	790	797	PF00069	0.557
MOD_N-GLC_1	140	145	PF02516	0.576
MOD_N-GLC_1	407	412	PF02516	0.632
MOD_N-GLC_1	794	799	PF02516	0.585
MOD_N-GLC_2	596	598	PF02516	0.506
MOD_NEK2_1	134	139	PF00069	0.502
MOD_NEK2_1	288	293	PF00069	0.551
MOD_NEK2_1	300	305	PF00069	0.419
MOD_NEK2_1	402	407	PF00069	0.614
MOD_NEK2_1	420	425	PF00069	0.690
MOD_NEK2_1	458	463	PF00069	0.456
MOD_NEK2_1	465	470	PF00069	0.396
MOD_NEK2_1	540	545	PF00069	0.412
MOD_NEK2_1	78	83	PF00069	0.612
MOD_NEK2_1	794	799	PF00069	0.596
MOD_NEK2_1	88	93	PF00069	0.566
MOD_PIKK_1	127	133	PF00454	0.505
MOD_PIKK_1	134	140	PF00454	0.467
MOD_PIKK_1	381	387	PF00454	0.594
MOD_PIKK_1	503	509	PF00454	0.375
MOD_PIKK_1	571	577	PF00454	0.402
MOD_PIKK_1	580	586	PF00454	0.410
MOD_PIKK_1	620	626	PF00454	0.611
MOD_PIKK_1	723	729	PF00454	0.504
MOD_PIKK_1	95	101	PF00454	0.550
MOD_PKA_1	266	272	PF00069	0.561
MOD_PKA_2	200	206	PF00069	0.568
MOD_PKA_2	266	272	PF00069	0.625
MOD_PKA_2	459	465	PF00069	0.457
MOD_PKA_2	540	546	PF00069	0.381
MOD_PKA_2	790	796	PF00069	0.650
MOD_PKB_1	804	812	PF00069	0.552
MOD_Plk_1	140	146	PF00069	0.533
MOD_Plk_1	402	408	PF00069	0.562
MOD_Plk_1	420	426	PF00069	0.706
MOD_Plk_1	485	491	PF00069	0.465
MOD_Plk_1	644	650	PF00069	0.450
MOD_Plk_1	794	800	PF00069	0.628
MOD_Plk_1	88	94	PF00069	0.567
MOD_Plk_2-3	196	202	PF00069	0.569
MOD_Plk_2-3	397	403	PF00069	0.629
MOD_Plk_2-3	481	487	PF00069	0.477
MOD_Plk_2-3	730	736	PF00069	0.578
MOD_Plk_4	288	294	PF00069	0.504
MOD_Plk_4	296	302	PF00069	0.497
MOD_Plk_4	485	491	PF00069	0.368
MOD_Plk_4	665	671	PF00069	0.524
MOD_Plk_4	701	707	PF00069	0.569
MOD_Plk_4	730	736	PF00069	0.544
MOD_Plk_4	82	88	PF00069	0.630
MOD_ProDKin_1	1	7	PF00069	0.664
MOD_ProDKin_1	646	652	PF00069	0.572
MOD_SUMO_for_1	146	149	PF00179	0.571
MOD_SUMO_rev_2	115	124	PF00179	0.508
MOD_SUMO_rev_2	169	174	PF00179	0.461
MOD_SUMO_rev_2	191	200	PF00179	0.551
MOD_SUMO_rev_2	259	268	PF00179	0.596
MOD_SUMO_rev_2	334	340	PF00179	0.568
TRG_DiLeu_BaEn_1	63	68	PF01217	0.515
TRG_DiLeu_BaEn_4	65	71	PF01217	0.623
TRG_DiLeu_BaLyEn_6	130	135	PF01217	0.511
TRG_ENDOCYTIC_2	211	214	PF00928	0.638
TRG_ENDOCYTIC_2	301	304	PF00928	0.447
TRG_ENDOCYTIC_2	467	470	PF00928	0.491
TRG_ENDOCYTIC_2	537	540	PF00928	0.455
TRG_ENDOCYTIC_2	732	735	PF00928	0.527
TRG_ENDOCYTIC_2	746	749	PF00928	0.470
TRG_ER_diArg_1	112	114	PF00400	0.626
TRG_ER_diArg_1	366	368	PF00400	0.552
TRG_ER_diArg_1	424	426	PF00400	0.622
TRG_ER_diArg_1	599	601	PF00400	0.538
TRG_ER_diArg_1	790	792	PF00400	0.627
TRG_NES_CRM1_1	631	645	PF08389	0.521
TRG_NES_CRM1_1	730	744	PF08389	0.580
TRG_Pf-PMV_PEXEL_1	133	138	PF00026	0.502
TRG_Pf-PMV_PEXEL_1	205	209	PF00026	0.546
TRG_Pf-PMV_PEXEL_1	234	238	PF00026	0.609
TRG_Pf-PMV_PEXEL_1	390	394	PF00026	0.580
TRG_Pf-PMV_PEXEL_1	824	828	PF00026	0.582

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PC98	Leptomonas seymouri	68%	100%
A0A0S4JP59	Bodo saltans	30%	88%
A0A1X0P3L6	Trypanosomatidae	45%	99%
A0A422NYC2	Trypanosoma rangeli	45%	98%
A4H451	Leishmania braziliensis	84%	100%
A4HSC8	Leishmania infantum	100%	100%
C9ZPY3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	99%
E9AKA9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
Q4QJJ8	Leishmania major	94%	100%
V5BAC3	Trypanosoma cruzi	43%	98%