Vacuolar protein sorting-associated protein 28 homolog

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

vacuolar protein sorting-associated protein-like protein

Species:

Leishmania donovani

UniProt:

A0A3Q8IWE4_LEIDO

TriTrypDb:

LdBPK_365400.1 , LdCL_360061200 , LDHU3_36.7220

Length:

209

Annotations

Annotations by Jardim et al.

Vacuolar proteins, Vacuolar sorting-associated -like

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	yes	yes: 4
Forrest at al. (procyclic)	yes	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 12

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0000813	ESCRT I complex	3	11
GO:0032991	protein-containing complex	1	11
GO:0036452	ESCRT complex	2	11
GO:0098796	membrane protein complex	2	11

Expansion

Sequence features

A0A3Q8IWE4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IWE4

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	11
GO:0006886	intracellular protein transport	4	11
GO:0007034	vacuolar transport	4	11
GO:0008104	protein localization	4	11
GO:0009987	cellular process	1	11
GO:0015031	protein transport	4	11
GO:0016192	vesicle-mediated transport	4	11
GO:0016197	endosomal transport	4	11
GO:0032509	endosome transport via multivesicular body sorting pathway	5	11
GO:0032511	late endosome to vacuole transport via multivesicular body sorting pathway	6	11
GO:0033036	macromolecule localization	2	11
GO:0033365	protein localization to organelle	5	11
GO:0043328	protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	5	11
GO:0045184	establishment of protein localization	3	11
GO:0045324	late endosome to vacuole transport	5	11
GO:0046907	intracellular transport	3	11
GO:0051179	localization	1	11
GO:0051234	establishment of localization	2	11
GO:0051641	cellular localization	2	11
GO:0051649	establishment of localization in cell	3	11
GO:0070727	cellular macromolecule localization	3	11
GO:0071702	organic substance transport	4	11
GO:0071705	nitrogen compound transport	4	11
GO:0071985	multivesicular body sorting pathway	5	11
GO:0072594	establishment of protein localization to organelle	4	11
GO:0072665	protein localization to vacuole	6	11
GO:0072666	establishment of protein localization to vacuole	5	11

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	1
GO:0044877	protein-containing complex binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	120	124	PF00656	0.409
CLV_C14_Caspase3-7	90	94	PF00656	0.520
CLV_NRD_NRD_1	146	148	PF00675	0.532
CLV_NRD_NRD_1	165	167	PF00675	0.494
CLV_NRD_NRD_1	35	37	PF00675	0.495
CLV_NRD_NRD_1	49	51	PF00675	0.371
CLV_PCSK_KEX2_1	165	167	PF00082	0.507
CLV_PCSK_KEX2_1	35	37	PF00082	0.495
CLV_PCSK_KEX2_1	49	51	PF00082	0.371
CLV_PCSK_SKI1_1	196	200	PF00082	0.553
CLV_PCSK_SKI1_1	49	53	PF00082	0.501
DOC_CYCLIN_RxL_1	144	155	PF00134	0.553
DOC_CYCLIN_RxL_1	46	53	PF00134	0.451
DOC_PP2B_LxvP_1	135	138	PF13499	0.520
DOC_USP7_MATH_1	108	112	PF00917	0.520
DOC_WW_Pin1_4	95	100	PF00397	0.503
LIG_14-3-3_CanoR_1	109	113	PF00244	0.520
LIG_14-3-3_CanoR_1	126	135	PF00244	0.520
LIG_BIR_II_1	1	5	PF00653	0.761
LIG_BRCT_BRCA1_1	114	118	PF00533	0.481
LIG_FHA_2	118	124	PF00498	0.409
LIG_FHA_2	128	134	PF00498	0.409
LIG_FHA_2	69	75	PF00498	0.419
LIG_FHA_2	88	94	PF00498	0.520
LIG_IBAR_NPY_1	67	69	PF08397	0.428
LIG_LIR_Gen_1	115	125	PF02991	0.520
LIG_LIR_Gen_1	189	199	PF02991	0.441
LIG_LIR_Gen_1	19	27	PF02991	0.409
LIG_LIR_Nem_3	115	121	PF02991	0.520
LIG_LIR_Nem_3	189	195	PF02991	0.441
LIG_LIR_Nem_3	19	24	PF02991	0.409
LIG_LIR_Nem_3	74	80	PF02991	0.403
LIG_NRP_CendR_1	208	209	PF00754	0.790
LIG_PCNA_yPIPBox_3	136	148	PF02747	0.520
LIG_PTB_Apo_2	64	71	PF02174	0.409
LIG_REV1ctd_RIR_1	74	83	PF16727	0.520
LIG_SH2_STAP1	44	48	PF00017	0.409
LIG_SH2_STAT5	16	19	PF00017	0.400
LIG_SH2_STAT5	69	72	PF00017	0.429
LIG_SH2_STAT5	80	83	PF00017	0.379
LIG_SH3_3	131	137	PF00018	0.520
LIG_SUMO_SIM_anti_2	130	136	PF11976	0.520
LIG_SUMO_SIM_par_1	23	28	PF11976	0.409
LIG_TRAF2_1	152	155	PF00917	0.339
LIG_UBA3_1	142	148	PF00899	0.462
MOD_CK1_1	201	207	PF00069	0.624
MOD_CK2_1	149	155	PF00069	0.503
MOD_CK2_1	95	101	PF00069	0.503
MOD_GlcNHglycan	170	173	PF01048	0.475
MOD_GlcNHglycan	177	181	PF01048	0.387
MOD_GSK3_1	108	115	PF00069	0.446
MOD_GSK3_1	117	124	PF00069	0.358
MOD_NEK2_1	121	126	PF00069	0.403
MOD_NEK2_1	127	132	PF00069	0.393
MOD_NEK2_1	176	181	PF00069	0.553
MOD_NEK2_2	44	49	PF00069	0.428
MOD_NEK2_2	87	92	PF00069	0.520
MOD_PKA_2	108	114	PF00069	0.504
MOD_PKA_2	87	93	PF00069	0.475
MOD_Plk_1	44	50	PF00069	0.417
MOD_ProDKin_1	95	101	PF00069	0.503
TRG_DiLeu_BaLyEn_6	47	52	PF01217	0.409
TRG_ENDOCYTIC_2	192	195	PF00928	0.409
TRG_ER_diArg_1	165	167	PF00400	0.520
TRG_ER_diArg_1	48	50	PF00400	0.451
TRG_NES_CRM1_1	141	155	PF08389	0.451
TRG_NES_CRM1_1	15	31	PF08389	0.409
TRG_Pf-PMV_PEXEL_1	105	110	PF00026	0.520
TRG_Pf-PMV_PEXEL_1	36	41	PF00026	0.409
TRG_Pf-PMV_PEXEL_1	49	53	PF00026	0.463

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HW69	Leptomonas seymouri	86%	100%
A0A0S4IXL0	Bodo saltans	32%	100%
A0A1X0P3A4	Trypanosomatidae	49%	97%
A0A3R7MQI1	Trypanosoma rangeli	49%	99%
A4HQ33	Leishmania braziliensis	91%	100%
A4IDT9	Leishmania infantum	100%	100%
A8XWB7	Caenorhabditis briggsae	32%	100%
B5DEN9	Rattus norvegicus	30%	92%
D0A8T3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	46%	96%
E9ATV1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
O65421	Arabidopsis thaliana	31%	100%
Q02767	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	86%
Q3T178	Bos taurus	30%	95%
Q4Q0P5	Leishmania major	95%	100%
Q54NF1	Dictyostelium discoideum	30%	73%
Q9D1C8	Mus musculus	30%	95%
Q9NA26	Caenorhabditis elegans	32%	100%
Q9S9T7	Arabidopsis thaliana	33%	100%
Q9UK41	Homo sapiens	30%	95%
Q9V359	Drosophila melanogaster	34%	100%
V5BRX4	Trypanosoma cruzi	51%	98%