Cop-coated_vesicle_membrane_protein_p24_precursor _putative/GeneDB:LmjF.32.2020

Quick info Annotations Localization Abundance Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cop-coated vesicle membrane protein p24 precursor, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8ITC4_LEIDO

TriTrypDb:

LdBPK_322150.1 , LdCL_320026900 , LDHU3_32.2670

Length:

160

Annotations

LeishMANIAdb annotations

Related to other Eukaryotic GOLD domain proteins, involved in sorting of GPI-achored proteins. Kinetoplastids contain several paralogous GOLD domain protein families, not all closely related to each others.. Localization: ER (by homology)

Annotations by Jardim et al.

Intracellular protein trafficking, Cop-coated vesicle membrane p24

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 6, no: 5
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	12
GO:0110165	cellular anatomical entity	1	12
GO:0005783	endoplasmic reticulum	5	1
GO:0005793	endoplasmic reticulum-Golgi intermediate compartment	5	1
GO:0005794	Golgi apparatus	5	1
GO:0030134	COPII-coated ER to Golgi transport vesicle	8	1
GO:0030135	coated vesicle	7	1
GO:0031410	cytoplasmic vesicle	6	1
GO:0031982	vesicle	4	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0097708	intracellular vesicle	5	1

Abundance

Amastigote (protein)


Source	Evidence on protein	Close homologs
Pescher et al. (upgregulation)	no	yes: 0

Promastigote and amastigote


Source	Evidence on protein	Close homologs
Lahav et al. - mRNA - Protein

Expansion

Sequence features

A0A3Q8ITC4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8ITC4

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	1
GO:0006886	intracellular protein transport	4	1
GO:0006888	endoplasmic reticulum to Golgi vesicle-mediated transport	4	1
GO:0006996	organelle organization	4	1
GO:0007030	Golgi organization	5	1
GO:0008104	protein localization	4	1
GO:0009987	cellular process	1	1
GO:0015031	protein transport	4	1
GO:0016043	cellular component organization	3	1
GO:0016192	vesicle-mediated transport	4	1
GO:0033036	macromolecule localization	2	1
GO:0045184	establishment of protein localization	3	1
GO:0046907	intracellular transport	3	1
GO:0048193	Golgi vesicle transport	5	1
GO:0051179	localization	1	1
GO:0051234	establishment of localization	2	1
GO:0051641	cellular localization	2	1
GO:0051649	establishment of localization in cell	3	1
GO:0070727	cellular macromolecule localization	3	1
GO:0071702	organic substance transport	4	1
GO:0071705	nitrogen compound transport	4	1
GO:0071840	cellular component organization or biogenesis	2	1

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_PCSK_KEX2_1	157	159	PF00082	0.369
CLV_PCSK_PC1ET2_1	157	159	PF00082	0.380
CLV_PCSK_SKI1_1	151	155	PF00082	0.226
CLV_PCSK_SKI1_1	37	41	PF00082	0.426
DEG_Nend_Nbox_1	1	3	PF02207	0.363
DOC_PP1_RVXF_1	125	132	PF00149	0.238
DOC_PP1_RVXF_1	22	29	PF00149	0.305
DOC_PP1_RVXF_1	35	41	PF00149	0.226
DOC_PP1_RVXF_1	60	67	PF00149	0.226
LIG_14-3-3_CanoR_1	55	60	PF00244	0.325
LIG_Actin_WH2_2	86	103	PF00022	0.251
LIG_BRCT_BRCA1_1	46	50	PF00533	0.328
LIG_CaM_IQ_9	93	109	PF13499	0.251
LIG_FHA_1	118	124	PF00498	0.241
LIG_FHA_1	58	64	PF00498	0.250
LIG_LIR_Gen_1	65	70	PF02991	0.313
LIG_MAD2	37	45	PF02301	0.251
LIG_SH2_STAP1	48	52	PF00017	0.328
LIG_SH2_STAT3	149	152	PF00017	0.421
LIG_SH2_STAT5	149	152	PF00017	0.278
LIG_SH3_3	75	81	PF00018	0.280
LIG_SxIP_EBH_1	37	50	PF03271	0.275
LIG_WRC_WIRS_1	63	68	PF05994	0.313
MOD_CK1_1	57	63	PF00069	0.231
MOD_GSK3_1	5	12	PF00069	0.232
MOD_GSK3_1	50	57	PF00069	0.228
MOD_GSK3_1	58	65	PF00069	0.230
MOD_GSK3_1	85	92	PF00069	0.313
MOD_NEK2_1	153	158	PF00069	0.575
MOD_NEK2_1	39	44	PF00069	0.223
MOD_NEK2_1	50	55	PF00069	0.209
MOD_NEK2_1	89	94	PF00069	0.251
MOD_PKA_2	117	123	PF00069	0.238
MOD_PKA_2	31	37	PF00069	0.249
MOD_PKA_2	54	60	PF00069	0.313
MOD_Plk_1	15	21	PF00069	0.223
MOD_Plk_1	22	28	PF00069	0.236
MOD_Plk_4	133	139	PF00069	0.393
MOD_Plk_4	143	149	PF00069	0.319
MOD_Plk_4	15	21	PF00069	0.251
MOD_Plk_4	22	28	PF00069	0.259
TRG_ER_diArg_1	110	113	PF00400	0.225
TRG_Pf-PMV_PEXEL_1	101	105	PF00026	0.524

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HZG2	Leptomonas seymouri	73%	73%
A0A0N1I2E2	Leptomonas seymouri	31%	75%
A0A0S4JG68	Bodo saltans	31%	72%
A0A0S4JHA6	Bodo saltans	31%	79%
A0A0S4JYK0	Bodo saltans	50%	79%
A0A1X0NPX7	Trypanosomatidae	30%	73%
A0A1X0NVX2	Trypanosomatidae	55%	70%
A0A422NB76	Trypanosoma rangeli	55%	73%
A0A422NFM3	Trypanosoma rangeli	26%	71%
A0AQ71	Drosophila simulans	24%	78%
A4HJ92	Leishmania braziliensis	32%	100%
A4HKJ2	Leishmania braziliensis	84%	100%
A4I6N6	Leishmania infantum	32%	77%
A4I824	Leishmania infantum	100%	100%
A7SXK3	Nematostella vectensis	35%	79%
B3LVB0	Drosophila ananassae	25%	78%
B3MTS8	Drosophila ananassae	25%	74%
B3NZM5	Drosophila erecta	24%	74%
B3P6T8	Drosophila erecta	24%	78%
B4GE47	Drosophila persimilis	24%	78%
B4GMC3	Drosophila persimilis	25%	74%
B4HJV5	Drosophila sechellia	24%	74%
B4JG34	Drosophila grimshawi	25%	74%
B4JYU5	Drosophila grimshawi	25%	78%
B4K4G5	Drosophila mojavensis	25%	78%
B4KB41	Drosophila mojavensis	25%	74%
B4LYB8	Drosophila virilis	25%	74%
B4MGF8	Drosophila virilis	25%	78%
B4NIY1	Drosophila willistoni	24%	78%
B4NKL0	Drosophila willistoni	25%	74%
B4PUZ3	Drosophila yakuba	24%	78%
B4PVC6	Drosophila yakuba	24%	74%
B4QWH9	Drosophila simulans	25%	74%
D0AA90	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	51%	70%
D3ZTX0	Rattus norvegicus	28%	71%
E9B2Y2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
O13770	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	76%
O17528	Caenorhabditis elegans	32%	79%
P32803	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	23%	79%
P38819	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	25%	75%
P39704	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	29%	74%
P49020	Cricetulus griseus	33%	82%
Q12450	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	77%
Q15363	Homo sapiens	33%	80%
Q28BQ6	Xenopus tropicalis	22%	73%
Q295B2	Drosophila pseudoobscura pseudoobscura	24%	74%
Q3T133	Bos taurus	26%	68%
Q4Q574	Leishmania major	97%	100%
Q4Q6F1	Leishmania major	31%	100%
Q54BN0	Dictyostelium discoideum	34%	77%
Q5I0E7	Rattus norvegicus	28%	68%
Q63524	Rattus norvegicus	33%	80%
Q6AY25	Rattus norvegicus	28%	72%
Q6BTC2	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	30%	75%
Q6IDL4	Arabidopsis thaliana	27%	74%
Q769F9	Dictyostelium discoideum	37%	78%
Q78IS1	Mus musculus	29%	72%
Q7Z7H5	Homo sapiens	28%	70%
Q8R1V4	Mus musculus	28%	70%
Q8SXY6	Drosophila melanogaster	24%	78%
Q99KF1	Mus musculus	26%	68%
Q9BVK6	Homo sapiens	26%	68%
Q9FVU0	Arabidopsis thaliana	26%	75%
Q9I7K5	Drosophila melanogaster	25%	74%
Q9LIL4	Arabidopsis thaliana	29%	75%
Q9P7I9	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	26%	80%
Q9R0Q3	Mus musculus	33%	80%
Q9Y3B3	Homo sapiens	28%	71%
Q9Y3Q3	Homo sapiens	27%	74%
V5BG96	Trypanosoma cruzi	55%	73%
V5BK13	Trypanosoma cruzi	25%	72%
V5BKG3	Trypanosoma cruzi	26%	71%