Protein disulfide-isomerase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

protein disulfide isomerase 2

Species:

Leishmania donovani

UniProt:

A0A3Q8IS34_LEIDO

TriTrypDb:

LdBPK_367280.1 , LdCL_360085400 , LDHU3_36.9660

Length:

477

Annotations

LeishMANIAdb annotations

Appears to be a family of secreted proteins, probably ER-localized, like its homologs in other Eukaryotes.. Seems to carry a putative KDEL-like signal on its C-terminus.. Localization: Secreted (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 1
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 7

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 10, no: 1
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005788	endoplasmic reticulum lumen	5	10
GO:0031974	membrane-enclosed lumen	2	10
GO:0043233	organelle lumen	3	10
GO:0070013	intracellular organelle lumen	4	10
GO:0110165	cellular anatomical entity	1	11
GO:0005783	endoplasmic reticulum	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0016020	membrane	2	1

Expansion

Sequence features

A0A3Q8IS34

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IS34

Function

Biological processes
Term	Name	Level	Count
GO:0006457	protein folding	2	1
GO:0006950	response to stress	2	1
GO:0009987	cellular process	1	1
GO:0033554	cellular response to stress	3	1
GO:0034976	response to endoplasmic reticulum stress	4	1
GO:0050896	response to stimulus	1	1
GO:0051716	cellular response to stimulus	2	1

Molecular functions
Term	Name	Level	Count
GO:0003756	protein disulfide isomerase activity	3	12
GO:0003824	catalytic activity	1	12
GO:0016853	isomerase activity	2	12
GO:0016860	intramolecular oxidoreductase activity	3	12
GO:0016864	intramolecular oxidoreductase activity, transposing S-S bonds	4	12
GO:0140096	catalytic activity, acting on a protein	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	278	282	PF00656	0.555
CLV_NRD_NRD_1	195	197	PF00675	0.432
CLV_NRD_NRD_1	309	311	PF00675	0.492
CLV_PCSK_KEX2_1	216	218	PF00082	0.341
CLV_PCSK_KEX2_1	309	311	PF00082	0.499
CLV_PCSK_PC1ET2_1	216	218	PF00082	0.341
CLV_PCSK_SKI1_1	216	220	PF00082	0.459
CLV_PCSK_SKI1_1	3	7	PF00082	0.659
CLV_PCSK_SKI1_1	331	335	PF00082	0.582
CLV_PCSK_SKI1_1	394	398	PF00082	0.375
DEG_APCC_DBOX_1	2	10	PF00400	0.692
DEG_Nend_UBRbox_3	1	3	PF02207	0.706
DOC_MAPK_gen_1	140	148	PF00069	0.571
DOC_MAPK_gen_1	243	252	PF00069	0.526
DOC_MAPK_gen_1	270	279	PF00069	0.437
DOC_MAPK_HePTP_8	267	279	PF00069	0.470
DOC_MAPK_MEF2A_6	270	279	PF00069	0.382
DOC_MAPK_MEF2A_6	89	96	PF00069	0.443
DOC_PP1_RVXF_1	243	250	PF00149	0.478
DOC_PP2B_LxvP_1	292	295	PF13499	0.493
DOC_PP4_FxxP_1	377	380	PF00568	0.408
DOC_PP4_FxxP_1	43	46	PF00568	0.443
DOC_USP7_MATH_1	188	192	PF00917	0.523
DOC_USP7_MATH_1	462	466	PF00917	0.747
DOC_USP7_UBL2_3	128	132	PF12436	0.571
DOC_USP7_UBL2_3	334	338	PF12436	0.623
DOC_USP7_UBL2_3	451	455	PF12436	0.518
DOC_USP7_UBL2_3	85	89	PF12436	0.452
DOC_WW_Pin1_4	181	186	PF00397	0.653
DOC_WW_Pin1_4	315	320	PF00397	0.477
LIG_14-3-3_CanoR_1	217	227	PF00244	0.498
LIG_BIR_III_2	344	348	PF00653	0.617
LIG_EH1_1	7	15	PF00400	0.626
LIG_eIF4E_1	271	277	PF01652	0.528
LIG_FHA_1	141	147	PF00498	0.651
LIG_FHA_1	219	225	PF00498	0.465
LIG_FHA_1	354	360	PF00498	0.461
LIG_FHA_1	368	374	PF00498	0.408
LIG_FHA_1	91	97	PF00498	0.408
LIG_FHA_2	276	282	PF00498	0.555
LIG_FHA_2	410	416	PF00498	0.493
LIG_FHA_2	444	450	PF00498	0.475
LIG_FHA_2	68	74	PF00498	0.555
LIG_FHA_2	75	81	PF00498	0.439
LIG_GBD_Chelix_1	165	173	PF00786	0.560
LIG_LIR_Apic_2	105	109	PF02991	0.493
LIG_LIR_Apic_2	428	433	PF02991	0.408
LIG_LIR_Gen_1	28	38	PF02991	0.468
LIG_LIR_Gen_1	4	13	PF02991	0.684
LIG_LIR_Nem_3	225	230	PF02991	0.470
LIG_LIR_Nem_3	233	238	PF02991	0.425
LIG_LIR_Nem_3	28	33	PF02991	0.468
LIG_LIR_Nem_3	283	288	PF02991	0.528
LIG_LIR_Nem_3	297	303	PF02991	0.422
LIG_LIR_Nem_3	315	320	PF02991	0.442
LIG_LIR_Nem_3	362	366	PF02991	0.519
LIG_LIR_Nem_3	4	8	PF02991	0.691
LIG_LIR_Nem_3	424	429	PF02991	0.416
LIG_OCRL_FandH_1	306	318	PF00620	0.438
LIG_PDZ_Class_3	472	477	PF00595	0.790
LIG_Pex14_2	303	307	PF04695	0.438
LIG_Pex14_2	377	381	PF04695	0.400
LIG_Pex14_2	43	47	PF04695	0.427
LIG_SH2_CRK	106	110	PF00017	0.528
LIG_SH2_CRK	285	289	PF00017	0.572
LIG_SH2_CRK	317	321	PF00017	0.341
LIG_SH2_NCK_1	317	321	PF00017	0.341
LIG_SH2_NCK_1	392	396	PF00017	0.475
LIG_SH2_STAP1	392	396	PF00017	0.465
LIG_SH2_STAT3	271	274	PF00017	0.528
LIG_SH2_STAT5	204	207	PF00017	0.509
LIG_SH2_STAT5	226	229	PF00017	0.477
LIG_SH2_STAT5	317	320	PF00017	0.495
LIG_SH2_STAT5	332	335	PF00017	0.570
LIG_SH2_STAT5	366	369	PF00017	0.462
LIG_SH2_STAT5	430	433	PF00017	0.420
LIG_SH2_STAT5	95	98	PF00017	0.555
LIG_SUMO_SIM_anti_2	37	42	PF11976	0.466
LIG_SUMO_SIM_par_1	263	269	PF11976	0.477
LIG_TRAF2_1	472	475	PF00917	0.793
LIG_TRAF2_1	77	80	PF00917	0.477
LIG_WRC_WIRS_1	276	281	PF05994	0.470
MOD_CK1_1	153	159	PF00069	0.522
MOD_CK1_1	160	166	PF00069	0.484
MOD_CK1_1	168	174	PF00069	0.439
MOD_CK1_1	398	404	PF00069	0.524
MOD_CK1_1	456	462	PF00069	0.644
MOD_CK1_1	469	475	PF00069	0.763
MOD_CK2_1	128	134	PF00069	0.488
MOD_CK2_1	469	475	PF00069	0.838
MOD_CK2_1	67	73	PF00069	0.545
MOD_CK2_1	74	80	PF00069	0.511
MOD_GlcNHglycan	152	155	PF01048	0.491
MOD_GlcNHglycan	18	21	PF01048	0.680
MOD_GlcNHglycan	34	37	PF01048	0.528
MOD_GlcNHglycan	422	426	PF01048	0.455
MOD_GlcNHglycan	460	463	PF01048	0.705
MOD_GlcNHglycan	464	467	PF01048	0.764
MOD_GlcNHglycan	468	471	PF01048	0.788
MOD_GSK3_1	153	160	PF00069	0.515
MOD_GSK3_1	32	39	PF00069	0.515
MOD_GSK3_1	421	428	PF00069	0.435
MOD_GSK3_1	458	465	PF00069	0.735
MOD_GSK3_1	74	81	PF00069	0.523
MOD_NEK2_1	1	6	PF00069	0.722
MOD_NEK2_1	218	223	PF00069	0.430
MOD_NEK2_1	260	265	PF00069	0.451
MOD_NEK2_1	409	414	PF00069	0.459
MOD_NEK2_1	421	426	PF00069	0.383
MOD_NEK2_1	453	458	PF00069	0.620
MOD_PIKK_1	168	174	PF00454	0.590
MOD_PIKK_1	270	276	PF00454	0.505
MOD_PIKK_1	367	373	PF00454	0.477
MOD_PKA_1	140	146	PF00069	0.549
MOD_Plk_1	36	42	PF00069	0.505
MOD_Plk_1	78	84	PF00069	0.559
MOD_Plk_4	114	120	PF00069	0.512
MOD_Plk_4	157	163	PF00069	0.604
MOD_Plk_4	190	196	PF00069	0.423
MOD_Plk_4	260	266	PF00069	0.527
MOD_Plk_4	275	281	PF00069	0.397
MOD_Plk_4	337	343	PF00069	0.614
MOD_Plk_4	354	360	PF00069	0.461
MOD_Plk_4	36	42	PF00069	0.460
MOD_Plk_4	425	431	PF00069	0.422
MOD_Plk_4	90	96	PF00069	0.424
MOD_ProDKin_1	181	187	PF00069	0.647
MOD_ProDKin_1	315	321	PF00069	0.477
MOD_SUMO_for_1	139	142	PF00179	0.540
MOD_SUMO_for_1	333	336	PF00179	0.630
MOD_SUMO_rev_2	296	301	PF00179	0.431
MOD_SUMO_rev_2	330	340	PF00179	0.563
MOD_SUMO_rev_2	448	453	PF00179	0.443
TRG_DiLeu_BaEn_4	134	140	PF01217	0.525
TRG_ENDOCYTIC_2	285	288	PF00928	0.519
TRG_ENDOCYTIC_2	317	320	PF00928	0.503
TRG_ENDOCYTIC_2	392	395	PF00928	0.465
TRG_NES_CRM1_1	167	180	PF08389	0.576

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P805	Leptomonas seymouri	67%	100%
A0A0S4KL52	Bodo saltans	40%	100%
A0A1X0NMX9	Trypanosomatidae	46%	99%
A0A3R7L858	Trypanosoma rangeli	44%	99%
A3KPF5	Arabidopsis thaliana	25%	89%
A4HQL6	Leishmania braziliensis	77%	99%
A4ICD5	Leishmania infantum	99%	100%
D0A3S0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	37%	96%
D3Z6P0	Mus musculus	31%	91%
D4B2L8	Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371)	34%	91%
E9AUD1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	99%
P04785	Rattus norvegicus	30%	94%
P05307	Bos taurus	30%	94%
P07237	Homo sapiens	30%	94%
P08003	Mus musculus	32%	75%
P09102	Gallus gallus	30%	93%
P09103	Mus musculus	31%	94%
P11598	Rattus norvegicus	32%	94%
P12865	Trypanosoma brucei brucei	37%	96%
P13667	Homo sapiens	33%	74%
P17967	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	31%	91%
P21195	Oryctolagus cuniculus	30%	94%
P27773	Mus musculus	32%	94%
P29828	Medicago sativa	32%	93%
P30101	Homo sapiens	32%	94%
P32474	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	25%	92%
P34329	Caenorhabditis elegans	32%	77%
P38657	Bos taurus	33%	94%
P38658	Schistosoma mansoni	30%	99%
P38659	Rattus norvegicus	31%	74%
P52588	Zea mays	33%	93%
P52589	Triticum aestivum	33%	93%
P54399	Drosophila melanogaster	32%	96%
P55059	Humicola insolens	35%	94%
P80284	Hordeum vulgare	33%	93%
Q00002	Alternaria alternata	27%	100%
Q00248	Aspergillus oryzae (strain ATCC 42149 / RIB 40)	34%	93%
Q0JD42	Oryza sativa subsp. japonica	23%	100%
Q10057	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	35%	97%
Q12730	Aspergillus niger	32%	93%
Q13087	Homo sapiens	32%	91%
Q17770	Caenorhabditis elegans	31%	97%
Q17967	Caenorhabditis elegans	31%	98%
Q29RV1	Bos taurus	33%	74%
Q2HWU2	Macaca fuscata fuscata	30%	94%
Q43116	Ricinus communis	31%	96%
Q4Q059	Leishmania major	94%	100%
Q4VIT4	Chlorocebus aethiops	32%	94%
Q53LQ0	Oryza sativa subsp. japonica	33%	93%
Q54EN4	Dictyostelium discoideum	30%	93%
Q5R5B6	Pongo abelii	30%	94%
Q5RCH2	Pongo abelii	32%	91%
Q5RDG4	Pongo abelii	32%	94%
Q5WA72	Oryza sativa subsp. japonica	25%	89%
Q5XI02	Rattus norvegicus	21%	81%
Q66GQ3	Arabidopsis thaliana	27%	89%
Q67IX6	Oryza sativa subsp. japonica	30%	85%
Q69ST6	Oryza sativa subsp. japonica	29%	88%
Q7XRB5	Oryza sativa subsp. japonica	30%	92%
Q8JG64	Gallus gallus	32%	94%
Q8N807	Homo sapiens	21%	82%
Q8R4U2	Cricetulus griseus	30%	94%
Q8VX13	Arabidopsis thaliana	30%	82%
Q95LM0	Macaca fascicularis	20%	82%
Q9DAN1	Mus musculus	21%	81%
Q9FF55	Arabidopsis thaliana	29%	80%
Q9SRG3	Arabidopsis thaliana	32%	94%
Q9XF61	Datisca glomerata	29%	94%
Q9XI01	Arabidopsis thaliana	32%	95%
V5BHP7	Trypanosoma cruzi	43%	100%