Enoyl-CoA_reductase_putative/GeneDB:LmjF.25.1770

Quick info Localization Abundance Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

3-oxo-5-alpha-steroid 4-dehydrogenase, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8INK5_LEIDO

TriTrypDb:

LdBPK_251850.1 , LdCL_250024200 , LDHU3_25.2290

Length:

306

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	11
GO:0110165	cellular anatomical entity	1	11
GO:0005737	cytoplasm	2	1
GO:0005777	peroxisome	6	1
GO:0020015	glycosome	7	1
GO:0042579	microbody	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0005789	endoplasmic reticulum membrane	4	1
GO:0031090	organelle membrane	3	1

Abundance

Amastigote (protein)


Source	Evidence on protein	Close homologs
Pescher et al. (upgregulation)	no	yes: 0

Promastigote and amastigote


Source	Evidence on protein	Close homologs
Lahav et al. - mRNA - Protein

Expansion

Sequence features

A0A3Q8INK5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8INK5

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	11
GO:0008152	metabolic process	1	11
GO:0044238	primary metabolic process	2	11
GO:0071704	organic substance metabolic process	2	11
GO:0000038	very long-chain fatty acid metabolic process	5	1
GO:0006082	organic acid metabolic process	3	1
GO:0006631	fatty acid metabolic process	4	1
GO:0006633	fatty acid biosynthetic process	5	1
GO:0008610	lipid biosynthetic process	4	1
GO:0009058	biosynthetic process	2	1
GO:0009987	cellular process	1	1
GO:0016053	organic acid biosynthetic process	4	1
GO:0019752	carboxylic acid metabolic process	5	1
GO:0032787	monocarboxylic acid metabolic process	6	1
GO:0042761	very long-chain fatty acid biosynthetic process	6	1
GO:0043436	oxoacid metabolic process	4	1
GO:0044237	cellular metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044255	cellular lipid metabolic process	3	1
GO:0044281	small molecule metabolic process	2	1
GO:0044283	small molecule biosynthetic process	3	1
GO:0046394	carboxylic acid biosynthetic process	5	1
GO:0072330	monocarboxylic acid biosynthetic process	6	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0003865	3-oxo-5-alpha-steroid 4-dehydrogenase activity	5	5
GO:0016229	steroid dehydrogenase activity	3	5
GO:0016491	oxidoreductase activity	2	11
GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors	3	11
GO:0033765	steroid dehydrogenase activity, acting on the CH-CH group of donors	4	5
GO:0016628	oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor	4	1
GO:0102758	very-long-chain enoyl-CoA reductase activity	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	7	11	PF00656	0.612
CLV_NRD_NRD_1	12	14	PF00675	0.417
CLV_NRD_NRD_1	222	224	PF00675	0.432
CLV_NRD_NRD_1	39	41	PF00675	0.306
CLV_PCSK_KEX2_1	146	148	PF00082	0.242
CLV_PCSK_PC1ET2_1	146	148	PF00082	0.242
CLV_PCSK_SKI1_1	165	169	PF00082	0.328
CLV_PCSK_SKI1_1	41	45	PF00082	0.301
CLV_PCSK_SKI1_1	96	100	PF00082	0.220
DEG_APCC_DBOX_1	60	68	PF00400	0.603
DEG_Nend_UBRbox_1	1	4	PF02207	0.490
DOC_MAPK_gen_1	13	19	PF00069	0.584
DOC_MAPK_gen_1	146	154	PF00069	0.486
DOC_MAPK_gen_1	296	304	PF00069	0.435
DOC_MAPK_gen_1	59	67	PF00069	0.585
DOC_MAPK_MEF2A_6	134	141	PF00069	0.390
DOC_MAPK_MEF2A_6	29	38	PF00069	0.528
DOC_MAPK_NFAT4_5	134	142	PF00069	0.390
DOC_PP4_FxxP_1	157	160	PF00568	0.508
DOC_PP4_FxxP_1	43	46	PF00568	0.606
DOC_USP7_MATH_1	195	199	PF00917	0.259
DOC_USP7_MATH_1	240	244	PF00917	0.305
DOC_WW_Pin1_4	184	189	PF00397	0.374
DOC_WW_Pin1_4	47	52	PF00397	0.642
LIG_14-3-3_CanoR_1	40	44	PF00244	0.497
LIG_APCC_ABBA_1	83	88	PF00400	0.459
LIG_BRCT_BRCA1_1	186	190	PF00533	0.301
LIG_BRCT_BRCA1_1	265	269	PF00533	0.276
LIG_FHA_1	130	136	PF00498	0.281
LIG_FHA_1	228	234	PF00498	0.234
LIG_FHA_1	259	265	PF00498	0.320
LIG_FHA_2	204	210	PF00498	0.274
LIG_FHA_2	22	28	PF00498	0.494
LIG_FHA_2	64	70	PF00498	0.619
LIG_GBD_Chelix_1	107	115	PF00786	0.390
LIG_LIR_Apic_2	102	106	PF02991	0.278
LIG_LIR_Apic_2	42	46	PF02991	0.599
LIG_LIR_Gen_1	176	185	PF02991	0.297
LIG_LIR_Gen_1	253	259	PF02991	0.292
LIG_LIR_Nem_3	176	180	PF02991	0.326
LIG_LIR_Nem_3	198	203	PF02991	0.328
LIG_LIR_Nem_3	253	257	PF02991	0.292
LIG_LIR_Nem_3	97	103	PF02991	0.305
LIG_PCNA_yPIPBox_3	231	242	PF02747	0.301
LIG_Pex14_2	140	144	PF04695	0.289
LIG_Pex14_2	153	157	PF04695	0.494
LIG_Pex14_2	200	204	PF04695	0.231
LIG_PTB_Apo_2	167	174	PF02174	0.425
LIG_PTB_Phospho_1	167	173	PF10480	0.425
LIG_REV1ctd_RIR_1	142	151	PF16727	0.308
LIG_SH2_CRK	103	107	PF00017	0.278
LIG_SH2_CRK	95	99	PF00017	0.425
LIG_SH2_PTP2	136	139	PF00017	0.351
LIG_SH2_SRC	32	35	PF00017	0.563
LIG_SH2_STAP1	131	135	PF00017	0.297
LIG_SH2_STAT5	100	103	PF00017	0.423
LIG_SH2_STAT5	131	134	PF00017	0.310
LIG_SH2_STAT5	136	139	PF00017	0.299
LIG_SH2_STAT5	183	186	PF00017	0.291
LIG_SH2_STAT5	247	250	PF00017	0.225
LIG_SH2_STAT5	288	291	PF00017	0.439
LIG_SH3_1	231	237	PF00018	0.256
LIG_SH3_2	160	165	PF14604	0.456
LIG_SH3_3	157	163	PF00018	0.494
LIG_SH3_3	231	237	PF00018	0.254
LIG_SH3_3	239	245	PF00018	0.121
LIG_SUMO_SIM_par_1	63	69	PF11976	0.543
LIG_TYR_ITIM	84	89	PF00017	0.493
LIG_TYR_ITIM	93	98	PF00017	0.448
LIG_UBA3_1	64	70	PF00899	0.591
LIG_WRC_WIRS_1	174	179	PF05994	0.278
MOD_CK1_1	158	164	PF00069	0.425
MOD_CK1_1	253	259	PF00069	0.338
MOD_CK1_1	47	53	PF00069	0.657
MOD_CK2_1	203	209	PF00069	0.330
MOD_GlcNHglycan	122	125	PF01048	0.559
MOD_GlcNHglycan	193	196	PF01048	0.465
MOD_GlcNHglycan	21	24	PF01048	0.275
MOD_GlcNHglycan	6	9	PF01048	0.322
MOD_GSK3_1	184	191	PF00069	0.378
MOD_GSK3_1	218	225	PF00069	0.294
MOD_GSK3_1	258	265	PF00069	0.283
MOD_NEK2_1	19	24	PF00069	0.584
MOD_NEK2_1	263	268	PF00069	0.289
MOD_NEK2_2	94	99	PF00069	0.465
MOD_PIKK_1	129	135	PF00454	0.297
MOD_PIKK_1	71	77	PF00454	0.550
MOD_PK_1	79	85	PF00069	0.538
MOD_PKA_2	115	121	PF00069	0.263
MOD_PKA_2	222	228	PF00069	0.320
MOD_PKA_2	39	45	PF00069	0.508
MOD_Plk_4	115	121	PF00069	0.274
MOD_Plk_4	148	154	PF00069	0.481
MOD_Plk_4	250	256	PF00069	0.240
MOD_Plk_4	263	269	PF00069	0.236
MOD_Plk_4	79	85	PF00069	0.587
MOD_ProDKin_1	184	190	PF00069	0.374
MOD_ProDKin_1	47	53	PF00069	0.644
MOD_SUMO_for_1	145	148	PF00179	0.450
MOD_SUMO_rev_2	22	31	PF00179	0.506
TRG_ENDOCYTIC_2	136	139	PF00928	0.325
TRG_ENDOCYTIC_2	174	177	PF00928	0.278
TRG_ENDOCYTIC_2	86	89	PF00928	0.499
TRG_ENDOCYTIC_2	95	98	PF00928	0.449
TRG_NLS_Bipartite_1	282	300	PF00514	0.458
TRG_NLS_MonoExtC_3	281	286	PF00514	0.505
TRG_NLS_MonoExtN_4	282	287	PF00514	0.491
TRG_NLS_MonoExtN_4	293	300	PF00514	0.452

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PF75	Leptomonas seymouri	73%	99%
A0A0S4IKY4	Bodo saltans	56%	100%
A0A1X0NYG0	Trypanosomatidae	60%	100%
A0A422NGL4	Trypanosoma rangeli	62%	100%
A4HEG5	Leishmania braziliensis	89%	100%
A4I1M5	Leishmania infantum	100%	100%
C9ZK67	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	63%	100%
E9AXQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
O94511	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	38%	100%
Q3SZ89	Bos taurus	30%	84%
Q3ZCD7	Bos taurus	37%	99%
Q4Q9R2	Leishmania major	96%	100%
Q55C17	Dictyostelium discoideum	38%	100%
Q5HYJ1	Homo sapiens	30%	84%
Q64232	Rattus norvegicus	36%	99%
Q68FF9	Mus musculus	22%	100%
Q8BFZ1	Mus musculus	34%	85%
Q99190	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	29%	99%
Q9CY27	Mus musculus	36%	99%
Q9M2U2	Arabidopsis thaliana	34%	99%
Q9N5Y2	Caenorhabditis elegans	37%	99%
Q9NZ01	Homo sapiens	37%	99%
Q9UT20	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	100%