Zinc finger, C3HC4 type (RING finger)/Ring finger domain/zinc-RING finger domain/Secreted protein acidic and rich in cysteine Ca binding region/EF-hand domain pair/EF-hand domain/EF hand, putative

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Zinc finger, C3HC4 type (RING finger)/EF-hand domain pair, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8IMS6_LEIDO

TriTrypDb:

LdBPK_241420.1 , LdCL_240019400 , LDHU3_24.1730

Length:

394

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 24
NetGPI	no	yes: 0, no: 24

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0031974	membrane-enclosed lumen	2	2
GO:0031981	nuclear lumen	5	2
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	2
GO:0043233	organelle lumen	3	2
GO:0070013	intracellular organelle lumen	4	2
GO:0097014	ciliary plasm	5	2
GO:0099568	cytoplasmic region	3	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

A0A3Q8IMS6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IMS6

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0016567	protein ubiquitination	7	2
GO:0019538	protein metabolic process	3	2
GO:0032446	protein modification by small protein conjugation	6	2
GO:0036211	protein modification process	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0044238	primary metabolic process	2	2
GO:0070647	protein modification by small protein conjugation or removal	5	2
GO:0071704	organic substance metabolic process	2	2
GO:1901564	organonitrogen compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	24
GO:0005509	calcium ion binding	5	18
GO:0043167	ion binding	2	24
GO:0043169	cation binding	3	24
GO:0046872	metal ion binding	4	24
GO:0003824	catalytic activity	1	2
GO:0004842	ubiquitin-protein transferase activity	4	2
GO:0016740	transferase activity	2	2
GO:0019787	ubiquitin-like protein transferase activity	3	2
GO:0061630	ubiquitin protein ligase activity	5	2
GO:0061659	ubiquitin-like protein ligase activity	4	2
GO:0140096	catalytic activity, acting on a protein	2	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	339	343	PF00656	0.429
CLV_C14_Caspase3-7	359	363	PF00656	0.284
CLV_NRD_NRD_1	76	78	PF00675	0.413
CLV_PCSK_KEX2_1	286	288	PF00082	0.421
CLV_PCSK_KEX2_1	76	78	PF00082	0.626
CLV_PCSK_PC1ET2_1	286	288	PF00082	0.421
CLV_PCSK_PC7_1	72	78	PF00082	0.309
CLV_PCSK_SKI1_1	286	290	PF00082	0.525
DOC_CYCLIN_RxL_1	271	281	PF00134	0.430
DOC_MAPK_gen_1	286	294	PF00069	0.571
DOC_MAPK_MEF2A_6	62	71	PF00069	0.485
DOC_PP1_RVXF_1	272	279	PF00149	0.447
DOC_PP4_FxxP_1	123	126	PF00568	0.571
DOC_USP7_MATH_1	215	219	PF00917	0.497
DOC_USP7_MATH_1	53	57	PF00917	0.382
DOC_USP7_MATH_1	95	99	PF00917	0.672
DOC_WW_Pin1_4	122	127	PF00397	0.805
DOC_WW_Pin1_4	136	141	PF00397	0.680
DOC_WW_Pin1_4	278	283	PF00397	0.577
DOC_WW_Pin1_4	96	101	PF00397	0.524
LIG_14-3-3_CanoR_1	234	239	PF00244	0.595
LIG_APCC_ABBA_1	252	257	PF00400	0.466
LIG_BIR_II_1	1	5	PF00653	0.563
LIG_FHA_1	13	19	PF00498	0.459
LIG_FHA_1	158	164	PF00498	0.483
LIG_FHA_1	196	202	PF00498	0.505
LIG_FHA_2	279	285	PF00498	0.457
LIG_FHA_2	337	343	PF00498	0.308
LIG_LIR_Apic_2	21	26	PF02991	0.323
LIG_LIR_Gen_1	244	254	PF02991	0.448
LIG_LIR_Gen_1	325	334	PF02991	0.364
LIG_LIR_Gen_1	349	357	PF02991	0.321
LIG_LIR_Gen_1	385	391	PF02991	0.412
LIG_LIR_Nem_3	147	153	PF02991	0.561
LIG_LIR_Nem_3	223	228	PF02991	0.611
LIG_LIR_Nem_3	244	249	PF02991	0.489
LIG_LIR_Nem_3	251	256	PF02991	0.404
LIG_LIR_Nem_3	307	311	PF02991	0.513
LIG_LIR_Nem_3	325	330	PF02991	0.408
LIG_LIR_Nem_3	349	353	PF02991	0.331
LIG_LIR_Nem_3	385	389	PF02991	0.354
LIG_LYPXL_yS_3	150	153	PF13949	0.549
LIG_SH2_CRK	246	250	PF00017	0.447
LIG_SH2_CRK	311	315	PF00017	0.548
LIG_SH2_CRK	327	331	PF00017	0.230
LIG_SH2_GRB2like	200	203	PF00017	0.519
LIG_SH2_NCK_1	311	315	PF00017	0.422
LIG_SH2_STAP1	246	250	PF00017	0.447
LIG_SH2_STAT3	127	130	PF00017	0.697
LIG_SH2_STAT3	200	203	PF00017	0.749
LIG_SH2_STAT3	211	214	PF00017	0.649
LIG_SH2_STAT5	127	130	PF00017	0.656
LIG_SH2_STAT5	200	203	PF00017	0.696
LIG_SH3_3	224	230	PF00018	0.643
LIG_SH3_3	97	103	PF00018	0.549
LIG_SH3_4	313	320	PF00018	0.571
LIG_SUMO_SIM_anti_2	25	31	PF11976	0.400
LIG_SUMO_SIM_par_1	378	385	PF11976	0.365
MOD_CK1_1	125	131	PF00069	0.709
MOD_CK1_1	191	197	PF00069	0.717
MOD_CK1_1	220	226	PF00069	0.690
MOD_CK1_1	96	102	PF00069	0.574
MOD_CK2_1	298	304	PF00069	0.419
MOD_CK2_1	379	385	PF00069	0.390
MOD_GlcNHglycan	133	136	PF01048	0.725
MOD_GlcNHglycan	155	158	PF01048	0.694
MOD_GlcNHglycan	165	168	PF01048	0.686
MOD_GlcNHglycan	177	180	PF01048	0.613
MOD_GlcNHglycan	184	187	PF01048	0.594
MOD_GlcNHglycan	203	206	PF01048	0.524
MOD_GlcNHglycan	208	211	PF01048	0.802
MOD_GlcNHglycan	260	263	PF01048	0.489
MOD_GlcNHglycan	295	299	PF01048	0.457
MOD_GSK3_1	125	132	PF00069	0.640
MOD_GSK3_1	136	143	PF00069	0.662
MOD_GSK3_1	153	160	PF00069	0.688
MOD_GSK3_1	163	170	PF00069	0.577
MOD_GSK3_1	187	194	PF00069	0.767
MOD_GSK3_1	294	301	PF00069	0.444
MOD_GSK3_1	322	329	PF00069	0.527
MOD_GSK3_1	8	15	PF00069	0.398
MOD_N-GLC_1	187	192	PF02516	0.692
MOD_N-GLC_1	201	206	PF02516	0.703
MOD_N-GLC_1	258	263	PF02516	0.454
MOD_N-GLC_1	60	65	PF02516	0.497
MOD_N-GLC_2	3	5	PF02516	0.365
MOD_NEK2_1	153	158	PF00069	0.793
MOD_NEK2_1	163	168	PF00069	0.552
MOD_NEK2_1	201	206	PF00069	0.593
MOD_NEK2_1	294	299	PF00069	0.396
MOD_NEK2_1	322	327	PF00069	0.583
MOD_NEK2_2	379	384	PF00069	0.373
MOD_OFUCOSY	92	97	PF10250	0.429
MOD_PIKK_1	109	115	PF00454	0.787
MOD_PIKK_1	125	131	PF00454	0.560
MOD_PIKK_1	140	146	PF00454	0.659
MOD_PIKK_1	157	163	PF00454	0.559
MOD_PIKK_1	320	326	PF00454	0.460
MOD_PKA_2	191	197	PF00069	0.723
MOD_PKA_2	54	60	PF00069	0.496
MOD_Plk_1	267	273	PF00069	0.394
MOD_Plk_1	348	354	PF00069	0.372
MOD_Plk_1	60	66	PF00069	0.526
MOD_Plk_2-3	8	14	PF00069	0.394
MOD_Plk_4	248	254	PF00069	0.478
MOD_Plk_4	304	310	PF00069	0.403
MOD_Plk_4	326	332	PF00069	0.333
MOD_ProDKin_1	122	128	PF00069	0.805
MOD_ProDKin_1	136	142	PF00069	0.680
MOD_ProDKin_1	278	284	PF00069	0.574
MOD_ProDKin_1	96	102	PF00069	0.525
TRG_DiLeu_BaEn_1	349	354	PF01217	0.438
TRG_ENDOCYTIC_2	150	153	PF00928	0.549
TRG_ENDOCYTIC_2	225	228	PF00928	0.491
TRG_ENDOCYTIC_2	246	249	PF00928	0.470
TRG_ENDOCYTIC_2	327	330	PF00928	0.437

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P229	Leptomonas seymouri	58%	100%
A0A0N1ILD7	Leptomonas seymouri	21%	87%
A0A0S4JBP2	Bodo saltans	26%	88%
A0A1X0NCU6	Trypanosomatidae	35%	100%
A0A1X0NTS2	Trypanosomatidae	27%	88%
A0A3S7WWY0	Leishmania donovani	25%	88%
A0A3S7WYG1	Leishmania donovani	68%	100%
A4HDK3	Leishmania braziliensis	75%	100%
A4HDK4	Leishmania braziliensis	75%	100%
A4HZP7	Leishmania infantum	24%	100%
A4I0X7	Leishmania infantum	98%	100%
A4I0X8	Leishmania infantum	69%	84%
C9ZWH1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	100%
E9AK36	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	100%
E9AVK3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	24%	100%
E9AWY1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
E9AWY2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	69%	85%
O97201	Leishmania major	30%	67%
Q4QAJ2	Leishmania major	70%	85%
Q4QAJ3	Leishmania major	95%	100%
Q4QBX6	Leishmania major	24%	100%
V5B9E6	Trypanosoma cruzi	23%	100%
V5BK04	Trypanosoma cruzi	33%	100%