Ubiquitin-conjugating_enzyme_putative/GeneDB:LmjF.33.2770

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-conjugating enzyme, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8IMQ9_LEIDO

TriTrypDb:

LdBPK_332910.1 , LdCL_330036200 , LDHU3_33.4060

Length:

241

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A0A3Q8IMQ9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IMQ9

Function

Biological processes
Term	Name	Level	Count
GO:0000209	protein polyubiquitination	8	1
GO:0006508	proteolysis	4	1
GO:0006511	ubiquitin-dependent protein catabolic process	7	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0009057	macromolecule catabolic process	4	1
GO:0009987	cellular process	1	1
GO:0016567	protein ubiquitination	7	1
GO:0019538	protein metabolic process	3	1
GO:0019941	modification-dependent protein catabolic process	6	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043632	modification-dependent macromolecule catabolic process	5	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0044265	obsolete cellular macromolecule catabolic process	4	1
GO:0051603	proteolysis involved in protein catabolic process	5	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901575	organic substance catabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	9
GO:0003824	catalytic activity	1	9
GO:0005488	binding	1	9
GO:0005524	ATP binding	5	9
GO:0016740	transferase activity	2	8
GO:0017076	purine nucleotide binding	4	9
GO:0030554	adenyl nucleotide binding	5	9
GO:0032553	ribonucleotide binding	3	9
GO:0032555	purine ribonucleotide binding	4	9
GO:0032559	adenyl ribonucleotide binding	5	9
GO:0035639	purine ribonucleoside triphosphate binding	4	9
GO:0036094	small molecule binding	2	9
GO:0043167	ion binding	2	9
GO:0043168	anion binding	3	9
GO:0097159	organic cyclic compound binding	2	9
GO:0097367	carbohydrate derivative binding	2	9
GO:1901265	nucleoside phosphate binding	3	9
GO:1901363	heterocyclic compound binding	2	9
GO:0004842	ubiquitin-protein transferase activity	4	1
GO:0016874	ligase activity	2	3
GO:0019787	ubiquitin-like protein transferase activity	3	1
GO:0061631	ubiquitin conjugating enzyme activity	5	1
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	1
GO:0140096	catalytic activity, acting on a protein	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	139	141	PF00675	0.335
CLV_NRD_NRD_1	210	212	PF00675	0.685
CLV_NRD_NRD_1	239	241	PF00675	0.728
CLV_PCSK_KEX2_1	210	212	PF00082	0.723
CLV_PCSK_KEX2_1	239	241	PF00082	0.662
CLV_PCSK_KEX2_1	94	96	PF00082	0.355
CLV_PCSK_PC1ET2_1	94	96	PF00082	0.355
CLV_PCSK_SKI1_1	104	108	PF00082	0.345
CLV_PCSK_SKI1_1	111	115	PF00082	0.320
CLV_PCSK_SKI1_1	129	133	PF00082	0.335
CLV_PCSK_SKI1_1	210	214	PF00082	0.711
CLV_PCSK_SKI1_1	235	239	PF00082	0.633
CLV_PCSK_SKI1_1	70	74	PF00082	0.335
DEG_APCC_DBOX_1	103	111	PF00400	0.368
DEG_APCC_DBOX_1	157	165	PF00400	0.642
DEG_SPOP_SBC_1	187	191	PF00917	0.554
DOC_CYCLIN_RxL_1	105	116	PF00134	0.335
DOC_CYCLIN_RxL_1	207	216	PF00134	0.709
DOC_MAPK_MEF2A_6	53	60	PF00069	0.416
DOC_MAPK_MEF2A_6	94	103	PF00069	0.398
DOC_PP4_FxxP_1	159	162	PF00568	0.681
DOC_SPAK_OSR1_1	53	57	PF12202	0.416
DOC_USP7_MATH_1	175	179	PF00917	0.715
DOC_USP7_MATH_1	182	186	PF00917	0.741
DOC_USP7_MATH_1	192	196	PF00917	0.770
DOC_USP7_MATH_1	200	204	PF00917	0.683
DOC_USP7_MATH_1	230	234	PF00917	0.700
DOC_USP7_MATH_1	30	34	PF00917	0.431
DOC_USP7_UBL2_3	232	236	PF12436	0.655
DOC_WW_Pin1_4	201	206	PF00397	0.575
DOC_WW_Pin1_4	44	49	PF00397	0.309
LIG_14-3-3_CanoR_1	158	162	PF00244	0.773
LIG_14-3-3_CanoR_1	210	215	PF00244	0.667
LIG_Actin_RPEL_3	88	107	PF02755	0.416
LIG_EVH1_2	68	72	PF00568	0.416
LIG_FHA_1	207	213	PF00498	0.794
LIG_FHA_1	98	104	PF00498	0.405
LIG_FHA_2	158	164	PF00498	0.764
LIG_FHA_2	211	217	PF00498	0.724
LIG_FHA_2	223	229	PF00498	0.718
LIG_Integrin_RGD_1	85	87	PF01839	0.416
LIG_LIR_Nem_3	133	138	PF02991	0.335
LIG_LIR_Nem_3	61	67	PF02991	0.335
LIG_LYPXL_yS_3	64	67	PF13949	0.335
LIG_REV1ctd_RIR_1	70	79	PF16727	0.416
LIG_SH2_CRK	145	149	PF00017	0.720
LIG_SH2_NCK_1	145	149	PF00017	0.720
LIG_SH2_STAP1	138	142	PF00017	0.416
LIG_SH2_STAT5	145	148	PF00017	0.720
LIG_SH3_2	65	70	PF14604	0.335
LIG_SH3_3	62	68	PF00018	0.313
MOD_CK1_1	171	177	PF00069	0.583
MOD_CK1_1	185	191	PF00069	0.770
MOD_CK1_1	195	201	PF00069	0.747
MOD_CK1_1	203	209	PF00069	0.450
MOD_CK2_1	157	163	PF00069	0.761
MOD_CK2_1	210	216	PF00069	0.665
MOD_CK2_1	222	228	PF00069	0.662
MOD_CK2_1	30	36	PF00069	0.441
MOD_GlcNHglycan	120	123	PF01048	0.313
MOD_GlcNHglycan	176	180	PF01048	0.720
MOD_GlcNHglycan	184	187	PF01048	0.680
MOD_GlcNHglycan	194	197	PF01048	0.629
MOD_GSK3_1	167	174	PF00069	0.712
MOD_GSK3_1	182	189	PF00069	0.523
MOD_GSK3_1	200	207	PF00069	0.694
MOD_N-GLC_1	200	205	PF02516	0.683
MOD_N-GLC_1	80	85	PF02516	0.368
MOD_NEK2_1	144	149	PF00069	0.624
MOD_NEK2_1	72	77	PF00069	0.368
MOD_PKA_1	210	216	PF00069	0.614
MOD_PKA_2	157	163	PF00069	0.699
MOD_PKA_2	210	216	PF00069	0.683
MOD_Plk_4	168	174	PF00069	0.528
MOD_ProDKin_1	201	207	PF00069	0.575
MOD_ProDKin_1	44	50	PF00069	0.309
MOD_SUMO_rev_2	133	143	PF00179	0.416
TRG_DiLeu_BaEn_1	126	131	PF01217	0.335
TRG_DiLeu_LyEn_5	126	131	PF01217	0.335
TRG_ENDOCYTIC_2	145	148	PF00928	0.699
TRG_ENDOCYTIC_2	64	67	PF00928	0.335
TRG_ER_diArg_1	210	212	PF00400	0.611
TRG_ER_diArg_1	238	240	PF00400	0.724
TRG_NLS_MonoExtN_4	232	238	PF00514	0.737
TRG_Pf-PMV_PEXEL_1	111	116	PF00026	0.335
TRG_Pf-PMV_PEXEL_1	129	133	PF00026	0.335
TRG_Pf-PMV_PEXEL_1	9	14	PF00026	0.377

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P683	Leptomonas seymouri	70%	100%
A0A0N1I1I8	Leptomonas seymouri	29%	100%
A0A1X0NSR5	Trypanosomatidae	58%	86%
A4H861	Leishmania braziliensis	80%	100%
A4I9B7	Leishmania infantum	100%	100%
E9B4B7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
E9B4R1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	83%
P49427	Homo sapiens	31%	100%
Q28F89	Xenopus tropicalis	37%	100%
Q29503	Oryctolagus cuniculus	31%	100%
Q32LD2	Bos taurus	29%	100%
Q4Q3B5	Leishmania major	27%	83%
Q4Q3Q8	Leishmania major	88%	100%
Q6ZWZ2	Mus musculus	31%	100%
Q712K3	Homo sapiens	31%	100%
Q7ZY08	Xenopus laevis	32%	100%
Q8CFI2	Mus musculus	31%	100%
Q8N2K1	Homo sapiens	22%	93%
Q9NPD8	Homo sapiens	28%	100%
V5BAJ3	Trypanosoma cruzi	49%	100%