tRNA pseudouridine synthase, putative

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA pseudouridine synthase, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8IKM7_LEIDO

TriTrypDb:

LdBPK_340330.1 , LdCL_340008400 , LDHU3_34.0480

Length:

701

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0043226	organelle	2	6
GO:0043227	membrane-bounded organelle	3	6
GO:0043229	intracellular organelle	3	6
GO:0043231	intracellular membrane-bounded organelle	4	6
GO:0110165	cellular anatomical entity	1	6
GO:0005634	nucleus	5	1
GO:0005737	cytoplasm	2	1

Expansion

Sequence features

A0A3Q8IKM7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IKM7

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	9
GO:0006139	nucleobase-containing compound metabolic process	3	9
GO:0006396	RNA processing	6	9
GO:0006399	tRNA metabolic process	7	9
GO:0006400	tRNA modification	6	6
GO:0006725	cellular aromatic compound metabolic process	3	9
GO:0006807	nitrogen compound metabolic process	2	9
GO:0008033	tRNA processing	8	9
GO:0008152	metabolic process	1	9
GO:0009451	RNA modification	5	9
GO:0009987	cellular process	1	9
GO:0016070	RNA metabolic process	5	9
GO:0031119	tRNA pseudouridine synthesis	7	6
GO:0034470	ncRNA processing	7	9
GO:0034641	cellular nitrogen compound metabolic process	3	9
GO:0034660	ncRNA metabolic process	6	9
GO:0043170	macromolecule metabolic process	3	9
GO:0043412	macromolecule modification	4	9
GO:0044237	cellular metabolic process	2	9
GO:0044238	primary metabolic process	2	9
GO:0046483	heterocycle metabolic process	3	9
GO:0071704	organic substance metabolic process	2	9
GO:0090304	nucleic acid metabolic process	4	9
GO:1901360	organic cyclic compound metabolic process	3	9
GO:0016071	mRNA metabolic process	6	1
GO:0016556	mRNA modification	6	1
GO:1990481	mRNA pseudouridine synthesis	7	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	9
GO:0003723	RNA binding	4	9
GO:0003824	catalytic activity	1	9
GO:0005488	binding	1	9
GO:0009982	pseudouridine synthase activity	4	9
GO:0016853	isomerase activity	2	9
GO:0016866	intramolecular transferase activity	3	9
GO:0097159	organic cyclic compound binding	2	9
GO:1901363	heterocyclic compound binding	2	9
GO:0106029	tRNA pseudouridine synthase activity	5	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	374	378	PF00656	0.634
CLV_NRD_NRD_1	219	221	PF00675	0.355
CLV_NRD_NRD_1	282	284	PF00675	0.664
CLV_NRD_NRD_1	289	291	PF00675	0.682
CLV_NRD_NRD_1	298	300	PF00675	0.680
CLV_NRD_NRD_1	53	55	PF00675	0.398
CLV_NRD_NRD_1	578	580	PF00675	0.283
CLV_NRD_NRD_1	603	605	PF00675	0.377
CLV_NRD_NRD_1	608	610	PF00675	0.372
CLV_NRD_NRD_1	631	633	PF00675	0.413
CLV_NRD_NRD_1	665	667	PF00675	0.360
CLV_PCSK_FUR_1	51	55	PF00082	0.522
CLV_PCSK_KEX2_1	145	147	PF00082	0.497
CLV_PCSK_KEX2_1	187	189	PF00082	0.393
CLV_PCSK_KEX2_1	219	221	PF00082	0.412
CLV_PCSK_KEX2_1	264	266	PF00082	0.724
CLV_PCSK_KEX2_1	281	283	PF00082	0.536
CLV_PCSK_KEX2_1	298	300	PF00082	0.534
CLV_PCSK_KEX2_1	50	52	PF00082	0.475
CLV_PCSK_KEX2_1	53	55	PF00082	0.383
CLV_PCSK_KEX2_1	534	536	PF00082	0.249
CLV_PCSK_KEX2_1	608	610	PF00082	0.373
CLV_PCSK_KEX2_1	631	633	PF00082	0.413
CLV_PCSK_KEX2_1	664	666	PF00082	0.362
CLV_PCSK_KEX2_1	697	699	PF00082	0.440
CLV_PCSK_PC1ET2_1	145	147	PF00082	0.455
CLV_PCSK_PC1ET2_1	187	189	PF00082	0.484
CLV_PCSK_PC1ET2_1	264	266	PF00082	0.724
CLV_PCSK_PC1ET2_1	298	300	PF00082	0.689
CLV_PCSK_PC1ET2_1	50	52	PF00082	0.511
CLV_PCSK_PC1ET2_1	534	536	PF00082	0.249
CLV_PCSK_PC1ET2_1	697	699	PF00082	0.345
CLV_PCSK_PC7_1	604	610	PF00082	0.377
CLV_PCSK_PC7_1	693	699	PF00082	0.316
CLV_PCSK_SKI1_1	187	191	PF00082	0.351
CLV_PCSK_SKI1_1	269	273	PF00082	0.776
CLV_PCSK_SKI1_1	300	304	PF00082	0.749
CLV_PCSK_SKI1_1	439	443	PF00082	0.344
CLV_PCSK_SKI1_1	599	603	PF00082	0.377
CLV_PCSK_SKI1_1	689	693	PF00082	0.424
CLV_PCSK_SKI1_1	89	93	PF00082	0.437
CLV_Separin_Metazoa	562	566	PF03568	0.283
DEG_ODPH_VHL_1	245	257	PF01847	0.455
DEG_SPOP_SBC_1	319	323	PF00917	0.561
DOC_CKS1_1	22	27	PF01111	0.541
DOC_CYCLIN_RxL_1	436	446	PF00134	0.422
DOC_CYCLIN_RxL_1	86	95	PF00134	0.436
DOC_MAPK_gen_1	50	60	PF00069	0.379
DOC_MAPK_gen_1	664	672	PF00069	0.369
DOC_MAPK_gen_1	686	694	PF00069	0.330
DOC_MAPK_MEF2A_6	127	136	PF00069	0.312
DOC_MAPK_MEF2A_6	53	62	PF00069	0.335
DOC_MAPK_MEF2A_6	664	672	PF00069	0.335
DOC_PP1_RVXF_1	251	258	PF00149	0.572
DOC_PP1_RVXF_1	691	697	PF00149	0.447
DOC_PP4_FxxP_1	244	247	PF00568	0.486
DOC_PP4_FxxP_1	499	502	PF00568	0.377
DOC_USP7_MATH_1	2	6	PF00917	0.631
DOC_USP7_MATH_1	289	293	PF00917	0.653
DOC_USP7_MATH_1	525	529	PF00917	0.304
DOC_USP7_UBL2_3	249	253	PF12436	0.380
DOC_WW_Pin1_4	21	26	PF00397	0.605
DOC_WW_Pin1_4	320	325	PF00397	0.728
DOC_WW_Pin1_4	348	353	PF00397	0.629
DOC_WW_Pin1_4	35	40	PF00397	0.592
DOC_WW_Pin1_4	452	457	PF00397	0.283
DOC_WW_Pin1_4	478	483	PF00397	0.355
DOC_WW_Pin1_4	498	503	PF00397	0.162
LIG_14-3-3_CanoR_1	115	123	PF00244	0.368
LIG_14-3-3_CanoR_1	127	133	PF00244	0.309
LIG_14-3-3_CanoR_1	269	274	PF00244	0.671
LIG_14-3-3_CanoR_1	354	360	PF00244	0.618
LIG_14-3-3_CanoR_1	467	473	PF00244	0.292
LIG_14-3-3_CanoR_1	505	511	PF00244	0.377
LIG_Actin_WH2_2	238	255	PF00022	0.460
LIG_Actin_WH2_2	678	695	PF00022	0.440
LIG_AP2alpha_2	476	478	PF02296	0.306
LIG_BRCT_BRCA1_1	474	478	PF00533	0.317
LIG_Clathr_ClatBox_1	110	114	PF01394	0.459
LIG_eIF4E_1	597	603	PF01652	0.294
LIG_FHA_1	225	231	PF00498	0.352
LIG_FHA_1	320	326	PF00498	0.667
LIG_FHA_1	335	341	PF00498	0.528
LIG_FHA_1	354	360	PF00498	0.548
LIG_FHA_1	517	523	PF00498	0.352
LIG_FHA_1	646	652	PF00498	0.583
LIG_FHA_1	92	98	PF00498	0.412
LIG_FHA_2	11	17	PF00498	0.696
LIG_FHA_2	230	236	PF00498	0.457
LIG_FHA_2	249	255	PF00498	0.317
LIG_FHA_2	270	276	PF00498	0.746
LIG_FHA_2	507	513	PF00498	0.317
LIG_FHA_2	80	86	PF00498	0.357
LIG_GBD_Chelix_1	668	676	PF00786	0.317
LIG_LIR_Apic_2	222	226	PF02991	0.309
LIG_LIR_Gen_1	227	236	PF02991	0.322
LIG_LIR_Gen_1	405	414	PF02991	0.431
LIG_LIR_Gen_1	448	456	PF02991	0.283
LIG_LIR_Gen_1	622	629	PF02991	0.316
LIG_LIR_Gen_1	99	110	PF02991	0.447
LIG_LIR_Nem_3	207	213	PF02991	0.353
LIG_LIR_Nem_3	239	244	PF02991	0.454
LIG_LIR_Nem_3	38	43	PF02991	0.538
LIG_LIR_Nem_3	448	454	PF02991	0.295
LIG_LIR_Nem_3	464	469	PF02991	0.283
LIG_LIR_Nem_3	622	626	PF02991	0.283
LIG_LIR_Nem_3	67	72	PF02991	0.345
LIG_LIR_Nem_3	79	83	PF02991	0.393
LIG_LIR_Nem_3	99	105	PF02991	0.267
LIG_LYPXL_yS_3	80	83	PF13949	0.400
LIG_MYND_1	28	32	PF01753	0.610
LIG_PCNA_yPIPBox_3	104	118	PF02747	0.464
LIG_RPA_C_Fungi	661	673	PF08784	0.357
LIG_SH2_CRK	201	205	PF00017	0.464
LIG_SH2_CRK	428	432	PF00017	0.393
LIG_SH2_CRK	466	470	PF00017	0.348
LIG_SH2_CRK	69	73	PF00017	0.336
LIG_SH2_GRB2like	213	216	PF00017	0.452
LIG_SH2_GRB2like	451	454	PF00017	0.283
LIG_SH2_NCK_1	201	205	PF00017	0.307
LIG_SH2_SRC	138	141	PF00017	0.457
LIG_SH2_STAP1	221	225	PF00017	0.307
LIG_SH2_STAP1	236	240	PF00017	0.358
LIG_SH2_STAP1	395	399	PF00017	0.634
LIG_SH2_STAP1	510	514	PF00017	0.377
LIG_SH2_STAT5	142	145	PF00017	0.471
LIG_SH2_STAT5	213	216	PF00017	0.422
LIG_SH2_STAT5	224	227	PF00017	0.342
LIG_SH2_STAT5	236	239	PF00017	0.410
LIG_SH2_STAT5	240	243	PF00017	0.404
LIG_SH2_STAT5	597	600	PF00017	0.294
LIG_SH2_STAT5	623	626	PF00017	0.317
LIG_SH2_STAT5	64	67	PF00017	0.365
LIG_SH2_STAT5	69	72	PF00017	0.375
LIG_SH3_1	26	32	PF00018	0.603
LIG_SH3_1	609	615	PF00018	0.377
LIG_SH3_3	131	137	PF00018	0.315
LIG_SH3_3	22	28	PF00018	0.670
LIG_SH3_3	244	250	PF00018	0.375
LIG_SH3_3	29	35	PF00018	0.625
LIG_SH3_3	349	355	PF00018	0.667
LIG_SH3_3	471	477	PF00018	0.283
LIG_SH3_3	609	615	PF00018	0.283
LIG_SH3_3	75	81	PF00018	0.425
LIG_SH3_4	249	256	PF00018	0.392
LIG_SUMO_SIM_anti_2	131	136	PF11976	0.407
LIG_SUMO_SIM_par_1	342	351	PF11976	0.682
LIG_SUMO_SIM_par_1	356	363	PF11976	0.681
LIG_SUMO_SIM_par_1	647	652	PF11976	0.575
LIG_TRAF2_1	13	16	PF00917	0.634
LIG_TRAF2_1	149	152	PF00917	0.448
LIG_TRAF2_1	274	277	PF00917	0.744
LIG_TRAF2_1	481	484	PF00917	0.326
LIG_TRAF2_1	509	512	PF00917	0.354
LIG_TRAF2_1	82	85	PF00917	0.373
LIG_UBA3_1	574	580	PF00899	0.283
LIG_WRC_WIRS_1	225	230	PF05994	0.306
LIG_WW_3	266	270	PF00397	0.630
MOD_CDK_SPK_2	21	26	PF00069	0.543
MOD_CDK_SPxK_1	348	354	PF00069	0.608
MOD_CDK_SPxK_1	35	41	PF00069	0.592
MOD_CDK_SPxxK_3	452	459	PF00069	0.283
MOD_CDK_SPxxK_3	498	505	PF00069	0.377
MOD_CK1_1	248	254	PF00069	0.439
MOD_CK1_1	348	354	PF00069	0.641
MOD_CK1_1	375	381	PF00069	0.660
MOD_CK1_1	490	496	PF00069	0.282
MOD_CK2_1	10	16	PF00069	0.590
MOD_CK2_1	179	185	PF00069	0.477
MOD_CK2_1	229	235	PF00069	0.408
MOD_CK2_1	248	254	PF00069	0.336
MOD_CK2_1	271	277	PF00069	0.755
MOD_CK2_1	320	326	PF00069	0.761
MOD_CK2_1	329	335	PF00069	0.727
MOD_CK2_1	368	374	PF00069	0.702
MOD_CK2_1	375	381	PF00069	0.607
MOD_CK2_1	478	484	PF00069	0.407
MOD_CK2_1	498	504	PF00069	0.198
MOD_CK2_1	506	512	PF00069	0.293
MOD_CK2_1	525	531	PF00069	0.317
MOD_CK2_1	79	85	PF00069	0.409
MOD_Cter_Amidation	217	220	PF01082	0.419
MOD_Cter_Amidation	262	265	PF01082	0.696
MOD_Cter_Amidation	532	535	PF01082	0.249
MOD_GlcNHglycan	174	177	PF01048	0.657
MOD_GlcNHglycan	287	290	PF01048	0.641
MOD_GlcNHglycan	330	334	PF01048	0.632
MOD_GlcNHglycan	4	7	PF01048	0.695
MOD_GlcNHglycan	539	542	PF01048	0.373
MOD_GlcNHglycan	625	629	PF01048	0.419
MOD_GSK3_1	285	292	PF00069	0.659
MOD_GSK3_1	368	375	PF00069	0.725
MOD_GSK3_1	457	464	PF00069	0.286
MOD_GSK3_1	468	475	PF00069	0.277
MOD_GSK3_1	512	519	PF00069	0.459
MOD_GSK3_1	91	98	PF00069	0.384
MOD_N-GLC_1	100	105	PF02516	0.408
MOD_N-GLC_1	452	457	PF02516	0.283
MOD_NEK2_1	407	412	PF00069	0.591
MOD_NEK2_1	413	418	PF00069	0.584
MOD_NEK2_1	468	473	PF00069	0.354
MOD_NEK2_1	487	492	PF00069	0.354
MOD_NEK2_1	619	624	PF00069	0.283
MOD_NEK2_2	461	466	PF00069	0.377
MOD_NEK2_2	684	689	PF00069	0.344
MOD_PIKK_1	372	378	PF00454	0.759
MOD_PKA_2	114	120	PF00069	0.365
MOD_PKA_2	289	295	PF00069	0.674
MOD_PKA_2	353	359	PF00069	0.615
MOD_Plk_1	461	467	PF00069	0.348
MOD_Plk_2-3	512	518	PF00069	0.348
MOD_Plk_2-3	79	85	PF00069	0.396
MOD_Plk_4	236	242	PF00069	0.418
MOD_Plk_4	407	413	PF00069	0.523
MOD_Plk_4	569	575	PF00069	0.283
MOD_Plk_4	593	599	PF00069	0.354
MOD_Plk_4	619	625	PF00069	0.283
MOD_ProDKin_1	21	27	PF00069	0.602
MOD_ProDKin_1	320	326	PF00069	0.729
MOD_ProDKin_1	348	354	PF00069	0.630
MOD_ProDKin_1	35	41	PF00069	0.592
MOD_ProDKin_1	452	458	PF00069	0.283
MOD_ProDKin_1	478	484	PF00069	0.355
MOD_ProDKin_1	498	504	PF00069	0.162
MOD_SUMO_rev_2	15	20	PF00179	0.637
MOD_SUMO_rev_2	182	189	PF00179	0.448
TRG_DiLeu_BaEn_1	185	190	PF01217	0.343
TRG_DiLeu_LyEn_5	185	190	PF01217	0.343
TRG_ENDOCYTIC_2	201	204	PF00928	0.372
TRG_ENDOCYTIC_2	42	45	PF00928	0.534
TRG_ENDOCYTIC_2	451	454	PF00928	0.283
TRG_ENDOCYTIC_2	466	469	PF00928	0.283
TRG_ENDOCYTIC_2	623	626	PF00928	0.317
TRG_ENDOCYTIC_2	69	72	PF00928	0.340
TRG_ENDOCYTIC_2	80	83	PF00928	0.379
TRG_ER_diArg_1	219	221	PF00400	0.435
TRG_ER_diArg_1	281	283	PF00400	0.597
TRG_ER_diArg_1	51	54	PF00400	0.527
TRG_ER_diArg_1	607	609	PF00400	0.373
TRG_ER_diArg_1	631	633	PF00400	0.415
TRG_ER_diArg_1	664	666	PF00400	0.400
TRG_NLS_Bipartite_1	281	302	PF00514	0.682
TRG_NLS_MonoExtC_3	49	54	PF00514	0.529
TRG_NLS_MonoExtN_4	281	287	PF00514	0.677
TRG_NLS_MonoExtN_4	297	302	PF00514	0.638
TRG_NLS_MonoExtN_4	51	58	PF00514	0.393
TRG_Pf-PMV_PEXEL_1	188	192	PF00026	0.440
TRG_Pf-PMV_PEXEL_1	439	444	PF00026	0.427
TRG_Pf-PMV_PEXEL_1	89	93	PF00026	0.437

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I609	Leptomonas seymouri	69%	100%
A0A422N797	Trypanosoma rangeli	42%	100%
A4H7D9	Leishmania braziliensis	80%	100%
A4I9K1	Leishmania infantum	100%	100%
D0A1W0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
E9B4J9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
Q4Q3H7	Leishmania major	95%	100%