tRNA_intron_endonuclease_catalytic_C-inal_domain_ containing_protein_putative/Pfam:PF01974

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA intron endonuclease, catalytic C-terminal domain containing protein, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8IKM3_LEIDO

TriTrypDb:

LdBPK_363920.1 , LdCL_360046200 , LDHU3_36.5260

Length:

181

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 8
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	2
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1

Expansion

Sequence features

A0A3Q8IKM3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IKM3

Function

Biological processes
Term	Name	Level	Count
GO:0000394	RNA splicing, via endonucleolytic cleavage and ligation	8	10
GO:0006139	nucleobase-containing compound metabolic process	3	10
GO:0006388	tRNA splicing, via endonucleolytic cleavage and ligation	9	10
GO:0006396	RNA processing	6	10
GO:0006399	tRNA metabolic process	7	10
GO:0006725	cellular aromatic compound metabolic process	3	10
GO:0006807	nitrogen compound metabolic process	2	10
GO:0008033	tRNA processing	8	10
GO:0008152	metabolic process	1	10
GO:0008380	RNA splicing	7	10
GO:0009987	cellular process	1	10
GO:0016070	RNA metabolic process	5	10
GO:0034470	ncRNA processing	7	10
GO:0034641	cellular nitrogen compound metabolic process	3	10
GO:0034660	ncRNA metabolic process	6	10
GO:0043170	macromolecule metabolic process	3	10
GO:0044237	cellular metabolic process	2	10
GO:0044238	primary metabolic process	2	10
GO:0046483	heterocycle metabolic process	3	10
GO:0071704	organic substance metabolic process	2	10
GO:0090304	nucleic acid metabolic process	4	10
GO:1901360	organic cyclic compound metabolic process	3	10

Molecular functions
Term	Name	Level	Count
GO:0000213	tRNA-intron endonuclease activity	6	10
GO:0003824	catalytic activity	1	10
GO:0004518	nuclease activity	4	10
GO:0004519	endonuclease activity	5	10
GO:0004521	RNA endonuclease activity	5	10
GO:0004540	RNA nuclease activity	4	10
GO:0004549	tRNA-specific ribonuclease activity	5	10
GO:0016787	hydrolase activity	2	10
GO:0016788	hydrolase activity, acting on ester bonds	3	10
GO:0016892	RNA endonuclease activity, producing 3'-phosphomonoesters	6	10
GO:0016894	endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters	6	10
GO:0140098	catalytic activity, acting on RNA	3	10
GO:0140101	catalytic activity, acting on a tRNA	4	10
GO:0140640	catalytic activity, acting on a nucleic acid	2	10
GO:0003676	nucleic acid binding	3	1
GO:0005488	binding	1	1
GO:0097159	organic cyclic compound binding	2	1
GO:1901363	heterocyclic compound binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	172	174	PF00675	0.621
CLV_NRD_NRD_1	178	180	PF00675	0.621
CLV_NRD_NRD_1	53	55	PF00675	0.604
CLV_NRD_NRD_1	72	74	PF00675	0.533
CLV_PCSK_KEX2_1	140	142	PF00082	0.420
CLV_PCSK_KEX2_1	172	174	PF00082	0.625
CLV_PCSK_KEX2_1	33	35	PF00082	0.596
CLV_PCSK_KEX2_1	53	55	PF00082	0.641
CLV_PCSK_KEX2_1	74	76	PF00082	0.508
CLV_PCSK_PC1ET2_1	140	142	PF00082	0.444
CLV_PCSK_PC1ET2_1	33	35	PF00082	0.596
CLV_PCSK_PC1ET2_1	74	76	PF00082	0.542
CLV_PCSK_PC7_1	136	142	PF00082	0.444
CLV_PCSK_SKI1_1	141	145	PF00082	0.429
CLV_Separin_Metazoa	70	74	PF03568	0.569
DEG_APCC_DBOX_1	140	148	PF00400	0.408
DEG_Nend_Nbox_1	1	3	PF02207	0.561
DEG_SCF_TRCP1_1	151	157	PF00400	0.587
DOC_MAPK_gen_1	140	147	PF00069	0.454
DOC_MAPK_gen_1	53	60	PF00069	0.547
DOC_MAPK_MEF2A_6	140	147	PF00069	0.444
DOC_PP4_FxxP_1	119	122	PF00568	0.401
DOC_USP7_MATH_1	174	178	PF00917	0.698
DOC_USP7_UBL2_3	176	180	PF12436	0.616
LIG_14-3-3_CanoR_1	133	139	PF00244	0.344
LIG_Actin_WH2_2	38	55	PF00022	0.485
LIG_Actin_WH2_2	79	97	PF00022	0.373
LIG_DCNL_PONY_1	1	4	PF03556	0.548
LIG_FHA_1	118	124	PF00498	0.468
LIG_FHA_1	155	161	PF00498	0.522
LIG_FHA_2	110	116	PF00498	0.452
LIG_FHA_2	144	150	PF00498	0.431
LIG_FHA_2	157	163	PF00498	0.399
LIG_HCF-1_HBM_1	89	92	PF13415	0.485
LIG_LIR_Gen_1	67	77	PF02991	0.570
LIG_LIR_Nem_3	67	72	PF02991	0.532
LIG_SH2_CRK	83	87	PF00017	0.401
LIG_SH2_SRC	107	110	PF00017	0.444
LIG_SH2_STAT5	104	107	PF00017	0.397
LIG_SH2_STAT5	23	26	PF00017	0.525
LIG_SUMO_SIM_anti_2	43	50	PF11976	0.539
LIG_SUMO_SIM_par_1	143	149	PF11976	0.405
LIG_SUMO_SIM_par_1	155	162	PF11976	0.509
LIG_TRAF2_1	146	149	PF00917	0.408
LIG_TRAF2_1	41	44	PF00917	0.409
LIG_TYR_ITIM	105	110	PF00017	0.369
MOD_CK1_1	152	158	PF00069	0.644
MOD_CK1_1	62	68	PF00069	0.585
MOD_CK2_1	109	115	PF00069	0.452
MOD_CK2_1	143	149	PF00069	0.474
MOD_CK2_1	38	44	PF00069	0.421
MOD_Cter_Amidation	138	141	PF01082	0.485
MOD_GlcNHglycan	151	154	PF01048	0.691
MOD_GlcNHglycan	16	19	PF01048	0.536
MOD_GlcNHglycan	35	38	PF01048	0.476
MOD_GlcNHglycan	61	64	PF01048	0.560
MOD_GSK3_1	149	156	PF00069	0.602
MOD_GSK3_1	43	50	PF00069	0.406
MOD_NEK2_1	117	122	PF00069	0.457
MOD_NEK2_1	143	148	PF00069	0.445
MOD_NEK2_1	164	169	PF00069	0.591
MOD_NEK2_1	47	52	PF00069	0.494
MOD_NEK2_2	174	179	PF00069	0.655
MOD_PIKK_1	47	53	PF00454	0.397
MOD_PIKK_1	95	101	PF00454	0.390
MOD_PKA_1	33	39	PF00069	0.514
MOD_PKA_2	33	39	PF00069	0.514
MOD_PKB_1	93	101	PF00069	0.408
MOD_Plk_1	43	49	PF00069	0.452
MOD_Plk_4	43	49	PF00069	0.491
TRG_DiLeu_BaEn_1	43	48	PF01217	0.527
TRG_DiLeu_BaEn_4	43	49	PF01217	0.416
TRG_ENDOCYTIC_2	107	110	PF00928	0.357
TRG_ENDOCYTIC_2	83	86	PF00928	0.361
TRG_ER_diArg_1	171	173	PF00400	0.650
TRG_ER_diArg_1	52	54	PF00400	0.560
TRG_ER_diArg_1	72	75	PF00400	0.501
TRG_ER_diArg_1	92	95	PF00400	0.155
TRG_NLS_MonoExtN_4	29	36	PF00514	0.573

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P4T2	Leptomonas seymouri	57%	85%
A0A0S4JEH5	Bodo saltans	27%	100%
A0A1X0P3G1	Trypanosomatidae	43%	87%
A4HPP1	Leishmania braziliensis	84%	100%
A4ID23	Leishmania infantum	100%	100%
D0A8H3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	72%
E9ATF5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
Q4Q142	Leishmania major	95%	100%