Vesicle-fusing ATPase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

vesicular-fusion protein nsf, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8IKK0_LEIDO

TriTrypDb:

LdBPK_200820.1 , LdCL_200012900 , LDHU3_20.1030

Length:

738

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 16

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 6

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 6

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	11
GO:0110165	cellular anatomical entity	1	12
GO:0005795	Golgi stack	4	1
GO:0031984	organelle subcompartment	2	1
GO:0098791	Golgi apparatus subcompartment	3	1
GO:0016020	membrane	2	1

Expansion

Sequence features

A0A3Q8IKK0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IKK0

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	12
GO:0008104	protein localization	4	12
GO:0009987	cellular process	1	12
GO:0015031	protein transport	4	12
GO:0016043	cellular component organization	3	12
GO:0022411	cellular component disassembly	4	12
GO:0032984	protein-containing complex disassembly	5	12
GO:0033036	macromolecule localization	2	12
GO:0035494	SNARE complex disassembly	6	12
GO:0043933	protein-containing complex organization	4	12
GO:0045184	establishment of protein localization	3	12
GO:0051179	localization	1	12
GO:0051234	establishment of localization	2	12
GO:0051641	cellular localization	2	12
GO:0070727	cellular macromolecule localization	3	12
GO:0071702	organic substance transport	4	12
GO:0071705	nitrogen compound transport	4	12
GO:0071840	cellular component organization or biogenesis	2	12
GO:0006891	intra-Golgi vesicle-mediated transport	6	1
GO:0006892	post-Golgi vesicle-mediated transport	6	1
GO:0006893	Golgi to plasma membrane transport	5	1
GO:0016192	vesicle-mediated transport	4	1
GO:0043001	Golgi to plasma membrane protein transport	5	1
GO:0048193	Golgi vesicle transport	5	1
GO:0051668	localization within membrane	3	1
GO:0061951	establishment of protein localization to plasma membrane	5	1
GO:0072657	protein localization to membrane	4	1
GO:0072659	protein localization to plasma membrane	5	1
GO:0090150	establishment of protein localization to membrane	4	1
GO:0098876	vesicle-mediated transport to the plasma membrane	4	1
GO:1990778	protein localization to cell periphery	5	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016462	pyrophosphatase activity	5	12
GO:0016787	hydrolase activity	2	12
GO:0016817	hydrolase activity, acting on acid anhydrides	3	12
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	12
GO:0016887	ATP hydrolysis activity	7	12
GO:0017076	purine nucleotide binding	4	12
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	355	359	PF00656	0.258
CLV_C14_Caspase3-7	505	509	PF00656	0.410
CLV_C14_Caspase3-7	647	651	PF00656	0.276
CLV_NRD_NRD_1	231	233	PF00675	0.359
CLV_NRD_NRD_1	269	271	PF00675	0.272
CLV_NRD_NRD_1	301	303	PF00675	0.286
CLV_NRD_NRD_1	499	501	PF00675	0.532
CLV_NRD_NRD_1	582	584	PF00675	0.251
CLV_NRD_NRD_1	72	74	PF00675	0.341
CLV_PCSK_FUR_1	516	520	PF00082	0.402
CLV_PCSK_KEX2_1	231	233	PF00082	0.353
CLV_PCSK_KEX2_1	499	501	PF00082	0.461
CLV_PCSK_KEX2_1	518	520	PF00082	0.443
CLV_PCSK_KEX2_1	529	531	PF00082	0.384
CLV_PCSK_KEX2_1	628	630	PF00082	0.242
CLV_PCSK_KEX2_1	72	74	PF00082	0.356
CLV_PCSK_PC1ET2_1	518	520	PF00082	0.419
CLV_PCSK_PC1ET2_1	529	531	PF00082	0.387
CLV_PCSK_PC1ET2_1	628	630	PF00082	0.242
CLV_PCSK_SKI1_1	176	180	PF00082	0.420
CLV_PCSK_SKI1_1	231	235	PF00082	0.342
CLV_PCSK_SKI1_1	237	241	PF00082	0.361
CLV_PCSK_SKI1_1	246	250	PF00082	0.374
CLV_PCSK_SKI1_1	253	257	PF00082	0.165
CLV_PCSK_SKI1_1	302	306	PF00082	0.242
CLV_PCSK_SKI1_1	445	449	PF00082	0.364
CLV_PCSK_SKI1_1	583	587	PF00082	0.242
CLV_PCSK_SKI1_1	635	639	PF00082	0.258
CLV_PCSK_SKI1_1	72	76	PF00082	0.377
DOC_ANK_TNKS_1	628	635	PF00023	0.276
DOC_CYCLIN_RxL_1	69	79	PF00134	0.372
DOC_CYCLIN_RxL_1	698	710	PF00134	0.349
DOC_MAPK_DCC_7	661	669	PF00069	0.343
DOC_MAPK_gen_1	173	183	PF00069	0.474
DOC_MAPK_gen_1	384	394	PF00069	0.248
DOC_MAPK_gen_1	529	536	PF00069	0.367
DOC_MAPK_MEF2A_6	134	143	PF00069	0.363
DOC_MAPK_MEF2A_6	387	396	PF00069	0.242
DOC_MAPK_MEF2A_6	529	538	PF00069	0.339
DOC_MAPK_MEF2A_6	661	669	PF00069	0.491
DOC_MAPK_MEF2A_6	674	683	PF00069	0.409
DOC_MAPK_MEF2A_6	703	711	PF00069	0.337
DOC_MAPK_RevD_3	255	271	PF00069	0.242
DOC_PP1_RVXF_1	602	609	PF00149	0.258
DOC_PP2B_LxvP_1	662	665	PF13499	0.345
DOC_SPAK_OSR1_1	387	391	PF12202	0.242
DOC_USP7_MATH_1	233	237	PF00917	0.319
DOC_USP7_MATH_1	66	70	PF00917	0.386
DOC_USP7_MATH_1	682	686	PF00917	0.396
DOC_USP7_MATH_1	731	735	PF00917	0.355
DOC_USP7_UBL2_3	246	250	PF12436	0.374
DOC_USP7_UBL2_3	514	518	PF12436	0.545
DOC_WW_Pin1_4	482	487	PF00397	0.524
DOC_WW_Pin1_4	589	594	PF00397	0.258
LIG_14-3-3_CanoR_1	134	142	PF00244	0.432
LIG_14-3-3_CanoR_1	148	154	PF00244	0.271
LIG_14-3-3_CanoR_1	176	183	PF00244	0.479
LIG_14-3-3_CanoR_1	3	8	PF00244	0.488
LIG_14-3-3_CanoR_1	614	620	PF00244	0.336
LIG_14-3-3_CanoR_1	720	726	PF00244	0.475
LIG_14-3-3_CanoR_1	82	86	PF00244	0.485
LIG_Actin_WH2_2	368	386	PF00022	0.247
LIG_APCC_ABBA_1	323	328	PF00400	0.242
LIG_APCC_ABBA_1	388	393	PF00400	0.242
LIG_APCC_ABBAyCdc20_2	387	393	PF00400	0.242
LIG_BIR_II_1	1	5	PF00653	0.475
LIG_BRCT_BRCA1_1	448	452	PF00533	0.242
LIG_Clathr_ClatBox_1	323	327	PF01394	0.242
LIG_DLG_GKlike_1	530	537	PF00625	0.482
LIG_FHA_1	134	140	PF00498	0.420
LIG_FHA_1	153	159	PF00498	0.245
LIG_FHA_1	16	22	PF00498	0.349
LIG_FHA_1	177	183	PF00498	0.385
LIG_FHA_1	370	376	PF00498	0.247
LIG_FHA_1	519	525	PF00498	0.466
LIG_FHA_1	82	88	PF00498	0.396
LIG_FHA_2	10	16	PF00498	0.413
LIG_FHA_2	182	188	PF00498	0.499
LIG_FHA_2	471	477	PF00498	0.356
LIG_GBD_Chelix_1	268	276	PF00786	0.242
LIG_LIR_Apic_2	587	593	PF02991	0.276
LIG_LIR_Gen_1	25	36	PF02991	0.514
LIG_LIR_Gen_1	288	294	PF02991	0.276
LIG_LIR_Gen_1	42	51	PF02991	0.415
LIG_LIR_Gen_1	558	567	PF02991	0.276
LIG_LIR_Gen_1	607	615	PF02991	0.266
LIG_LIR_Gen_1	643	654	PF02991	0.258
LIG_LIR_Gen_1	724	731	PF02991	0.436
LIG_LIR_Nem_3	129	135	PF02991	0.389
LIG_LIR_Nem_3	2	7	PF02991	0.494
LIG_LIR_Nem_3	225	229	PF02991	0.317
LIG_LIR_Nem_3	25	31	PF02991	0.489
LIG_LIR_Nem_3	288	293	PF02991	0.242
LIG_LIR_Nem_3	327	332	PF02991	0.242
LIG_LIR_Nem_3	42	46	PF02991	0.420
LIG_LIR_Nem_3	508	513	PF02991	0.402
LIG_LIR_Nem_3	558	564	PF02991	0.277
LIG_LIR_Nem_3	607	611	PF02991	0.266
LIG_MAD2	98	106	PF02301	0.327
LIG_NRBOX	619	625	PF00104	0.242
LIG_NRBOX	704	710	PF00104	0.474
LIG_PCNA_yPIPBox_3	614	624	PF02747	0.242
LIG_Pex14_2	226	230	PF04695	0.317
LIG_Rb_LxCxE_1	274	288	PF01857	0.388
LIG_SH2_CRK	258	262	PF00017	0.236
LIG_SH2_CRK	590	594	PF00017	0.276
LIG_SH2_GRB2like	615	618	PF00017	0.242
LIG_SH2_PTP2	561	564	PF00017	0.258
LIG_SH2_STAP1	520	524	PF00017	0.480
LIG_SH2_STAP1	615	619	PF00017	0.242
LIG_SH2_STAT3	128	131	PF00017	0.449
LIG_SH2_STAT3	520	523	PF00017	0.399
LIG_SH2_STAT5	132	135	PF00017	0.371
LIG_SH2_STAT5	520	523	PF00017	0.398
LIG_SH2_STAT5	552	555	PF00017	0.263
LIG_SH2_STAT5	561	564	PF00017	0.217
LIG_SH3_2	397	402	PF14604	0.412
LIG_SH3_3	28	34	PF00018	0.428
LIG_SH3_3	281	287	PF00018	0.242
LIG_SH3_3	394	400	PF00018	0.417
LIG_SH3_3	662	668	PF00018	0.310
LIG_SH3_3	83	89	PF00018	0.487
LIG_SUMO_SIM_anti_2	532	539	PF11976	0.366
LIG_SUMO_SIM_anti_2	690	697	PF11976	0.409
LIG_SUMO_SIM_par_1	622	627	PF11976	0.309
LIG_SUMO_SIM_par_1	636	643	PF11976	0.235
LIG_SUMO_SIM_par_1	705	710	PF11976	0.334
LIG_SUMO_SIM_par_1	83	88	PF11976	0.398
LIG_SxIP_EBH_1	665	674	PF03271	0.438
LIG_TRAF2_1	424	427	PF00917	0.388
LIG_TRAF2_1	480	483	PF00917	0.360
LIG_TRAF2_1	491	494	PF00917	0.418
LIG_UBA3_1	619	628	PF00899	0.242
LIG_UBA3_1	707	716	PF00899	0.417
MOD_CK1_1	165	171	PF00069	0.567
MOD_CK1_1	186	192	PF00069	0.370
MOD_CK1_1	421	427	PF00069	0.361
MOD_CK1_1	544	550	PF00069	0.292
MOD_CK1_1	592	598	PF00069	0.257
MOD_CK1_1	6	12	PF00069	0.370
MOD_CK1_1	691	697	PF00069	0.488
MOD_CK2_1	181	187	PF00069	0.480
MOD_CK2_1	421	427	PF00069	0.398
MOD_CK2_1	433	439	PF00069	0.194
MOD_Cter_Amidation	527	530	PF01082	0.455
MOD_Cter_Amidation	633	636	PF01082	0.276
MOD_GlcNHglycan	107	110	PF01048	0.511
MOD_GlcNHglycan	128	131	PF01048	0.364
MOD_GlcNHglycan	144	147	PF01048	0.412
MOD_GlcNHglycan	163	167	PF01048	0.438
MOD_GlcNHglycan	187	191	PF01048	0.527
MOD_GlcNHglycan	435	438	PF01048	0.242
MOD_GlcNHglycan	543	546	PF01048	0.246
MOD_GlcNHglycan	549	552	PF01048	0.269
MOD_GlcNHglycan	565	568	PF01048	0.432
MOD_GlcNHglycan	669	672	PF01048	0.378
MOD_GlcNHglycan	717	720	PF01048	0.547
MOD_GSK3_1	149	156	PF00069	0.483
MOD_GSK3_1	158	165	PF00069	0.554
MOD_GSK3_1	172	179	PF00069	0.297
MOD_GSK3_1	417	424	PF00069	0.558
MOD_GSK3_1	429	436	PF00069	0.121
MOD_GSK3_1	50	57	PF00069	0.480
MOD_GSK3_1	663	670	PF00069	0.351
MOD_GSK3_1	76	83	PF00069	0.376
MOD_GSK3_1	9	16	PF00069	0.394
MOD_N-GLC_1	13	18	PF02516	0.511
MOD_N-GLC_1	194	199	PF02516	0.534
MOD_N-GLC_1	433	438	PF02516	0.242
MOD_NEK2_1	133	138	PF00069	0.375
MOD_NEK2_1	149	154	PF00069	0.250
MOD_NEK2_1	624	629	PF00069	0.331
MOD_NEK2_1	75	80	PF00069	0.392
MOD_NEK2_2	656	661	PF00069	0.430
MOD_PIKK_1	133	139	PF00454	0.379
MOD_PIKK_1	176	182	PF00454	0.485
MOD_PIKK_1	606	612	PF00454	0.388
MOD_PIKK_1	682	688	PF00454	0.400
MOD_PK_1	3	9	PF00069	0.473
MOD_PKA_1	518	524	PF00069	0.409
MOD_PKA_2	133	139	PF00069	0.359
MOD_PKA_2	149	155	PF00069	0.301
MOD_PKA_2	172	178	PF00069	0.464
MOD_PKA_2	339	345	PF00069	0.258
MOD_PKA_2	518	524	PF00069	0.487
MOD_PKA_2	613	619	PF00069	0.242
MOD_PKA_2	81	87	PF00069	0.489
MOD_Plk_1	316	322	PF00069	0.302
MOD_Plk_1	421	427	PF00069	0.340
MOD_Plk_1	606	612	PF00069	0.253
MOD_Plk_4	149	155	PF00069	0.438
MOD_Plk_4	239	245	PF00069	0.358
MOD_Plk_4	263	269	PF00069	0.242
MOD_Plk_4	615	621	PF00069	0.308
MOD_Plk_4	691	697	PF00069	0.488
MOD_Plk_4	81	87	PF00069	0.406
MOD_ProDKin_1	482	488	PF00069	0.522
MOD_ProDKin_1	589	595	PF00069	0.258
MOD_SUMO_for_1	491	494	PF00179	0.418
MOD_SUMO_rev_2	157	164	PF00179	0.567
MOD_SUMO_rev_2	274	284	PF00179	0.388
MOD_SUMO_rev_2	710	718	PF00179	0.459
MOD_SUMO_rev_2	9	18	PF00179	0.494
TRG_DiLeu_BaEn_1	633	638	PF01217	0.243
TRG_DiLeu_BaEn_4	493	499	PF01217	0.399
TRG_DiLeu_BaLyEn_6	601	606	PF01217	0.336
TRG_DiLeu_BaLyEn_6	70	75	PF01217	0.355
TRG_DiLeu_BaLyEn_6	718	723	PF01217	0.469
TRG_ENDOCYTIC_2	132	135	PF00928	0.476
TRG_ENDOCYTIC_2	4	7	PF00928	0.483
TRG_ENDOCYTIC_2	561	564	PF00928	0.242
TRG_ENDOCYTIC_2	573	576	PF00928	0.242
TRG_ENDOCYTIC_2	646	649	PF00928	0.276
TRG_ENDOCYTIC_2	725	728	PF00928	0.491
TRG_ER_diArg_1	230	232	PF00400	0.364
TRG_ER_diArg_1	498	500	PF00400	0.529
TRG_ER_diArg_1	72	74	PF00400	0.356
TRG_Pf-PMV_PEXEL_1	270	274	PF00026	0.251
TRG_Pf-PMV_PEXEL_1	302	307	PF00026	0.289
TRG_Pf-PMV_PEXEL_1	98	103	PF00026	0.384

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P640	Leptomonas seymouri	83%	100%
A0A0N0P6X6	Leptomonas seymouri	33%	72%
A0A0S4JAU3	Bodo saltans	26%	100%
A0A0S4JTC3	Bodo saltans	57%	100%
A0A1X0NXR8	Trypanosomatidae	63%	100%
A0A1X0P437	Trypanosomatidae	29%	79%
A0A3S7X0L3	Leishmania donovani	26%	100%
A0A422MWC2	Trypanosoma rangeli	28%	83%
A0A422NPR7	Trypanosoma rangeli	63%	100%
A4HFM9	Leishmania braziliensis	26%	100%
A4HFN1	Leishmania braziliensis	34%	74%
A4HYS3	Leishmania infantum	100%	100%
A4I2Q7	Leishmania infantum	26%	100%
C9ZI97	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	58%	100%
D0A5W2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	83%
E9AD83	Leishmania major	26%	100%
E9AID2	Leishmania braziliensis	94%	100%
E9AUL9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
E9AZ07	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	100%
O14325	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	24%	95%
O28972	Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16)	32%	100%
P18708	Cricetulus griseus	40%	99%
P18759	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	40%	97%
P34732	Candida albicans	42%	93%
P46459	Homo sapiens	40%	99%
P46460	Mus musculus	40%	99%
P46461	Drosophila melanogaster	41%	99%
P54351	Drosophila melanogaster	42%	98%
Q07844	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	88%
Q0DGP6	Oryza sativa subsp. japonica	44%	99%
Q4QCW5	Leishmania major	98%	100%
Q54SY2	Dictyostelium discoideum	24%	85%
Q58556	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	30%	82%
Q5R410	Pongo abelii	40%	99%
Q75JI3	Dictyostelium discoideum	44%	100%
Q94392	Caenorhabditis elegans	44%	90%
Q9M0Y8	Arabidopsis thaliana	44%	99%
Q9NAG4	Caenorhabditis elegans	26%	91%
Q9P7Q4	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	41%	93%
Q9QUL6	Rattus norvegicus	42%	99%
V5DS04	Trypanosoma cruzi	64%	92%