PUF1, putative

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

PUF1, putative

Gene product:

PUF1, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8IK20_LEIDO

TriTrypDb:

LdBPK_360050.1 , LdCL_360005300 , LDHU3_36.0090

Length:

553

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 17
NetGPI	no	yes: 0, no: 18

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	3
GO:0016020	membrane	2	1

Expansion

Sequence features

A0A3Q8IK20

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IK20

Function

Biological processes
Term	Name	Level	Count
GO:0010468	regulation of gene expression	5	2
GO:0010608	post-transcriptional regulation of gene expression	6	2
GO:0019222	regulation of metabolic process	3	2
GO:0050789	regulation of biological process	2	2
GO:0060255	regulation of macromolecule metabolic process	4	2
GO:0065007	biological regulation	1	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	19
GO:0003723	RNA binding	4	19
GO:0005488	binding	1	19
GO:0097159	organic cyclic compound binding	2	19
GO:1901363	heterocyclic compound binding	2	19
GO:0003729	mRNA binding	5	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	13	15	PF00675	0.466
CLV_NRD_NRD_1	442	444	PF00675	0.459
CLV_NRD_NRD_1	449	451	PF00675	0.493
CLV_PCSK_KEX2_1	13	15	PF00082	0.484
CLV_PCSK_KEX2_1	44	46	PF00082	0.625
CLV_PCSK_KEX2_1	442	444	PF00082	0.476
CLV_PCSK_KEX2_1	449	451	PF00082	0.517
CLV_PCSK_PC1ET2_1	44	46	PF00082	0.685
CLV_PCSK_PC7_1	445	451	PF00082	0.599
CLV_PCSK_SKI1_1	104	108	PF00082	0.419
CLV_PCSK_SKI1_1	113	117	PF00082	0.445
CLV_PCSK_SKI1_1	140	144	PF00082	0.660
CLV_PCSK_SKI1_1	224	228	PF00082	0.462
CLV_PCSK_SKI1_1	343	347	PF00082	0.400
CLV_PCSK_SKI1_1	364	368	PF00082	0.338
CLV_PCSK_SKI1_1	374	378	PF00082	0.311
CLV_PCSK_SKI1_1	389	393	PF00082	0.401
CLV_PCSK_SKI1_1	449	453	PF00082	0.619
CLV_PCSK_SKI1_1	69	73	PF00082	0.527
CLV_PCSK_SKI1_1	75	79	PF00082	0.517
DEG_APCC_DBOX_1	342	350	PF00400	0.395
DEG_APCC_DBOX_1	74	82	PF00400	0.508
DEG_COP1_1	324	334	PF00400	0.473
DOC_CYCLIN_RxL_1	443	456	PF00134	0.472
DOC_CYCLIN_yCln2_LP_2	472	475	PF00134	0.618
DOC_PP2B_LxvP_1	381	384	PF13499	0.439
DOC_PP2B_LxvP_1	472	475	PF13499	0.733
DOC_PP4_FxxP_1	148	151	PF00568	0.553
DOC_PP4_FxxP_1	510	513	PF00568	0.705
DOC_USP7_MATH_1	122	126	PF00917	0.415
DOC_USP7_MATH_1	182	186	PF00917	0.403
DOC_USP7_MATH_1	422	426	PF00917	0.524
DOC_USP7_MATH_1	490	494	PF00917	0.674
DOC_USP7_UBL2_3	435	439	PF12436	0.422
DOC_WW_Pin1_4	367	372	PF00397	0.374
DOC_WW_Pin1_4	427	432	PF00397	0.409
LIG_14-3-3_CanoR_1	183	191	PF00244	0.389
LIG_Actin_WH2_2	284	299	PF00022	0.373
LIG_Actin_WH2_2	354	369	PF00022	0.294
LIG_Actin_WH2_2	413	430	PF00022	0.435
LIG_Actin_WH2_2	61	77	PF00022	0.416
LIG_AP2alpha_2	301	303	PF02296	0.290
LIG_BIR_II_1	1	5	PF00653	0.570
LIG_BIR_III_2	329	333	PF00653	0.467
LIG_BIR_III_4	121	125	PF00653	0.477
LIG_BIR_III_4	70	74	PF00653	0.454
LIG_BRCT_BRCA1_1	227	231	PF00533	0.489
LIG_BRCT_BRCA1_1	26	30	PF00533	0.442
LIG_CaM_IQ_9	84	100	PF13499	0.489
LIG_EVH1_2	535	539	PF00568	0.613
LIG_FHA_1	137	143	PF00498	0.571
LIG_FHA_1	200	206	PF00498	0.470
LIG_FHA_1	283	289	PF00498	0.363
LIG_FHA_1	336	342	PF00498	0.400
LIG_FHA_2	141	147	PF00498	0.655
LIG_FHA_2	150	156	PF00498	0.606
LIG_FHA_2	192	198	PF00498	0.397
LIG_FHA_2	404	410	PF00498	0.363
LIG_FHA_2	44	50	PF00498	0.644
LIG_LIR_Apic_2	146	151	PF02991	0.551
LIG_LIR_Apic_2	509	513	PF02991	0.734
LIG_LIR_Gen_1	199	209	PF02991	0.470
LIG_LIR_Gen_1	228	238	PF02991	0.525
LIG_LIR_Gen_1	249	260	PF02991	0.387
LIG_LIR_Gen_1	499	508	PF02991	0.698
LIG_LIR_Nem_3	196	201	PF02991	0.460
LIG_LIR_Nem_3	228	234	PF02991	0.496
LIG_LIR_Nem_3	249	255	PF02991	0.374
LIG_LIR_Nem_3	301	306	PF02991	0.340
LIG_LIR_Nem_3	392	398	PF02991	0.300
LIG_LIR_Nem_3	499	503	PF02991	0.663
LIG_MYND_1	471	475	PF01753	0.744
LIG_PCNA_yPIPBox_3	214	226	PF02747	0.363
LIG_PCNA_yPIPBox_3	504	515	PF02747	0.599
LIG_Pex14_2	103	107	PF04695	0.422
LIG_PTAP_UEV_1	331	336	PF05743	0.477
LIG_PTB_Apo_2	426	433	PF02174	0.383
LIG_PTB_Phospho_1	426	432	PF10480	0.388
LIG_SH2_CRK	201	205	PF00017	0.486
LIG_SH2_CRK	476	480	PF00017	0.650
LIG_SH2_GRB2like	337	340	PF00017	0.403
LIG_SH2_NCK_1	248	252	PF00017	0.424
LIG_SH2_STAP1	201	205	PF00017	0.486
LIG_SH2_STAP1	248	252	PF00017	0.468
LIG_SH2_STAP1	337	341	PF00017	0.430
LIG_SH2_STAT3	306	309	PF00017	0.299
LIG_SH2_STAT3	337	340	PF00017	0.486
LIG_SH2_STAT3	398	401	PF00017	0.285
LIG_SH2_STAT5	201	204	PF00017	0.456
LIG_SH2_STAT5	306	309	PF00017	0.281
LIG_SH2_STAT5	337	340	PF00017	0.411
LIG_SH2_STAT5	398	401	PF00017	0.283
LIG_SH2_STAT5	86	89	PF00017	0.548
LIG_SH3_3	201	207	PF00018	0.488
LIG_SH3_3	296	302	PF00018	0.309
LIG_SH3_3	329	335	PF00018	0.501
LIG_SH3_3	468	474	PF00018	0.680
LIG_SH3_3	529	535	PF00018	0.717
LIG_SUMO_SIM_anti_2	159	167	PF11976	0.405
LIG_SUMO_SIM_par_1	159	167	PF11976	0.405
LIG_TRAF2_1	194	197	PF00917	0.285
LIG_TRAF2_1	2	5	PF00917	0.491
LIG_TRAF2_1	467	470	PF00917	0.729
LIG_UBA3_1	420	428	PF00899	0.444
LIG_WRC_WIRS_1	507	512	PF05994	0.653
MOD_CDK_SPxxK_3	367	374	PF00069	0.412
MOD_CK1_1	237	243	PF00069	0.624
MOD_CK1_1	405	411	PF00069	0.362
MOD_CK1_1	438	444	PF00069	0.553
MOD_CK2_1	149	155	PF00069	0.665
MOD_CK2_1	191	197	PF00069	0.301
MOD_CK2_1	200	206	PF00069	0.340
MOD_CK2_1	318	324	PF00069	0.540
MOD_CK2_1	43	49	PF00069	0.636
MOD_GlcNHglycan	136	139	PF01048	0.614
MOD_GlcNHglycan	227	230	PF01048	0.525
MOD_GlcNHglycan	238	242	PF01048	0.564
MOD_GlcNHglycan	482	485	PF01048	0.758
MOD_GlcNHglycan	51	54	PF01048	0.616
MOD_GlcNHglycan	546	549	PF01048	0.715
MOD_GSK3_1	136	143	PF00069	0.595
MOD_GSK3_1	167	174	PF00069	0.430
MOD_GSK3_1	19	26	PF00069	0.492
MOD_GSK3_1	220	227	PF00069	0.426
MOD_GSK3_1	283	290	PF00069	0.371
MOD_GSK3_1	30	37	PF00069	0.533
MOD_GSK3_1	399	406	PF00069	0.297
MOD_N-GLC_1	122	127	PF02516	0.408
MOD_N-GLC_1	218	223	PF02516	0.389
MOD_N-GLC_1	292	297	PF02516	0.426
MOD_N-GLC_1	318	323	PF02516	0.579
MOD_N-GLC_1	335	340	PF02516	0.374
MOD_N-GLC_1	49	54	PF02516	0.623
MOD_N-GLC_1	544	549	PF02516	0.729
MOD_NEK2_1	134	139	PF00069	0.528
MOD_NEK2_1	225	230	PF00069	0.456
MOD_NEK2_1	292	297	PF00069	0.392
MOD_NEK2_1	366	371	PF00069	0.440
MOD_NEK2_1	391	396	PF00069	0.333
MOD_PIKK_1	182	188	PF00454	0.412
MOD_PIKK_1	411	417	PF00454	0.353
MOD_PIKK_1	459	465	PF00454	0.689
MOD_PKA_2	182	188	PF00069	0.411
MOD_Plk_1	218	224	PF00069	0.453
MOD_Plk_1	24	30	PF00069	0.492
MOD_Plk_1	292	298	PF00069	0.499
MOD_Plk_1	490	496	PF00069	0.741
MOD_Plk_4	200	206	PF00069	0.428
MOD_Plk_4	283	289	PF00069	0.308
MOD_Plk_4	422	428	PF00069	0.431
MOD_Plk_4	506	512	PF00069	0.645
MOD_ProDKin_1	367	373	PF00069	0.374
MOD_ProDKin_1	427	433	PF00069	0.403
MOD_SUMO_for_1	434	437	PF00179	0.469
MOD_SUMO_rev_2	2	8	PF00179	0.507
MOD_SUMO_rev_2	48	52	PF00179	0.691
TRG_DiLeu_BaEn_1	160	165	PF01217	0.375
TRG_DiLeu_BaLyEn_6	130	135	PF01217	0.447
TRG_DiLeu_BaLyEn_6	447	452	PF01217	0.473
TRG_DiLeu_BaLyEn_6	468	473	PF01217	0.615
TRG_ENDOCYTIC_2	201	204	PF00928	0.489
TRG_ENDOCYTIC_2	248	251	PF00928	0.353
TRG_ER_diArg_1	442	445	PF00400	0.493
TRG_ER_diArg_1	449	451	PF00400	0.539
TRG_Pf-PMV_PEXEL_1	166	171	PF00026	0.481
TRG_Pf-PMV_PEXEL_1	19	23	PF00026	0.437
TRG_Pf-PMV_PEXEL_1	374	379	PF00026	0.331
TRG_Pf-PMV_PEXEL_1	45	49	PF00026	0.682

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P473	Leptomonas seymouri	80%	99%
A0A0S4IY52	Bodo saltans	29%	78%
A0A0S4JMB8	Bodo saltans	46%	79%
A0A1X0P8D8	Trypanosomatidae	46%	96%
A0A3R7NHA6	Trypanosoma rangeli	46%	97%
A0A422N8V0	Trypanosoma rangeli	29%	100%
A4HNL0	Leishmania braziliensis	90%	100%
A4ICY3	Leishmania infantum	100%	100%
C9ZII7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	82%
D0A0Z9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	28%	95%
D0A2G7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	97%
E9ASC2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
E9AUS6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
Q4Q274	Leishmania major	98%	100%
Q4QCQ8	Leishmania major	24%	100%
V5B1W9	Trypanosoma cruzi	29%	99%
V5BV90	Trypanosoma cruzi	46%	91%