Folylpolyglutamate synthetase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

folylpolyglutamate synthetase

Species:

Leishmania donovani

UniProt:

A0A3Q8IJ59_LEIDO

TriTrypDb:

LdBPK_362740.1 , LdCL_360033800 , LDHU3_36.3780

Length:

540

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0005739	mitochondrion	5	1
GO:0005829	cytosol	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A0A3Q8IJ59

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IJ59

Function

Biological processes
Term	Name	Level	Count
GO:0006575	cellular modified amino acid metabolic process	3	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006760	folic acid-containing compound metabolic process	4	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009058	biosynthetic process	2	1
GO:0009396	folic acid-containing compound biosynthetic process	5	1
GO:0009987	cellular process	1	1
GO:0018130	heterocycle biosynthetic process	4	1
GO:0019438	aromatic compound biosynthetic process	4	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0042398	cellular modified amino acid biosynthetic process	4	1
GO:0042558	pteridine-containing compound metabolic process	4	1
GO:0042559	pteridine-containing compound biosynthetic process	5	1
GO:0044237	cellular metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044271	cellular nitrogen compound biosynthetic process	4	1
GO:0046483	heterocycle metabolic process	3	1
GO:0046900	tetrahydrofolylpolyglutamate metabolic process	5	1
GO:0046901	tetrahydrofolylpolyglutamate biosynthetic process	6	1
GO:0071704	organic substance metabolic process	2	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901362	organic cyclic compound biosynthetic process	4	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901566	organonitrogen compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004326	tetrahydrofolylpolyglutamate synthase activity	5	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016874	ligase activity	2	12
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	12
GO:0016881	acid-amino acid ligase activity	4	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	151	155	PF00656	0.484
CLV_C14_Caspase3-7	65	69	PF00656	0.524
CLV_NRD_NRD_1	290	292	PF00675	0.462
CLV_NRD_NRD_1	405	407	PF00675	0.350
CLV_NRD_NRD_1	537	539	PF00675	0.559
CLV_NRD_NRD_1	61	63	PF00675	0.564
CLV_NRD_NRD_1	79	81	PF00675	0.695
CLV_PCSK_KEX2_1	405	407	PF00082	0.360
CLV_PCSK_KEX2_1	417	419	PF00082	0.378
CLV_PCSK_KEX2_1	537	539	PF00082	0.560
CLV_PCSK_KEX2_1	60	62	PF00082	0.595
CLV_PCSK_KEX2_1	79	81	PF00082	0.683
CLV_PCSK_KEX2_1	92	94	PF00082	0.527
CLV_PCSK_PC1ET2_1	417	419	PF00082	0.479
CLV_PCSK_PC1ET2_1	60	62	PF00082	0.595
CLV_PCSK_PC1ET2_1	92	94	PF00082	0.507
CLV_PCSK_PC7_1	75	81	PF00082	0.546
CLV_PCSK_SKI1_1	198	202	PF00082	0.350
CLV_PCSK_SKI1_1	283	287	PF00082	0.397
CLV_PCSK_SKI1_1	325	329	PF00082	0.305
CLV_PCSK_SKI1_1	348	352	PF00082	0.357
CLV_PCSK_SKI1_1	405	409	PF00082	0.379
CLV_PCSK_SKI1_1	417	421	PF00082	0.339
CLV_PCSK_SKI1_1	463	467	PF00082	0.405
CLV_PCSK_SKI1_1	476	480	PF00082	0.496
CLV_PCSK_SKI1_1	527	531	PF00082	0.430
CLV_PCSK_SKI1_1	64	68	PF00082	0.535
DOC_CDC14_PxL_1	98	106	PF14671	0.481
DOC_CYCLIN_RxL_1	400	413	PF00134	0.393
DOC_MAPK_gen_1	291	299	PF00069	0.466
DOC_MAPK_gen_1	64	72	PF00069	0.578
DOC_MAPK_gen_1	92	100	PF00069	0.493
DOC_MAPK_MEF2A_6	198	207	PF00069	0.382
DOC_MAPK_MEF2A_6	291	300	PF00069	0.392
DOC_MAPK_MEF2A_6	308	315	PF00069	0.410
DOC_PP1_RVXF_1	104	110	PF00149	0.311
DOC_PP1_RVXF_1	404	411	PF00149	0.348
DOC_PP1_RVXF_1	95	101	PF00149	0.410
DOC_PP2B_LxvP_1	496	499	PF13499	0.497
DOC_PP4_FxxP_1	139	142	PF00568	0.326
DOC_PP4_FxxP_1	272	275	PF00568	0.419
DOC_USP7_MATH_1	12	16	PF00917	0.689
DOC_USP7_MATH_1	189	193	PF00917	0.505
DOC_USP7_MATH_1	306	310	PF00917	0.502
DOC_USP7_MATH_1	357	361	PF00917	0.409
DOC_USP7_MATH_1	425	429	PF00917	0.402
DOC_USP7_MATH_1	499	503	PF00917	0.518
DOC_USP7_UBL2_3	288	292	PF12436	0.426
DOC_USP7_UBL2_3	417	421	PF12436	0.484
DOC_WW_Pin1_4	138	143	PF00397	0.328
DOC_WW_Pin1_4	17	22	PF00397	0.742
DOC_WW_Pin1_4	187	192	PF00397	0.565
LIG_14-3-3_CanoR_1	442	450	PF00244	0.609
LIG_14-3-3_CanoR_1	463	471	PF00244	0.472
LIG_14-3-3_CanoR_1	485	494	PF00244	0.516
LIG_14-3-3_CanoR_1	61	67	PF00244	0.561
LIG_BIR_II_1	1	5	PF00653	0.600
LIG_Clathr_ClatBox_1	297	301	PF01394	0.427
LIG_DLG_GKlike_1	62	70	PF00625	0.479
LIG_EH1_1	519	527	PF00400	0.311
LIG_FHA_1	123	129	PF00498	0.288
LIG_FHA_1	227	233	PF00498	0.282
LIG_FHA_1	280	286	PF00498	0.375
LIG_FHA_1	370	376	PF00498	0.368
LIG_FHA_2	149	155	PF00498	0.484
LIG_FHA_2	251	257	PF00498	0.318
LIG_FHA_2	362	368	PF00498	0.387
LIG_FHA_2	410	416	PF00498	0.441
LIG_GBD_Chelix_1	521	529	PF00786	0.335
LIG_HP1_1	296	300	PF01393	0.472
LIG_LIR_Gen_1	172	179	PF02991	0.421
LIG_LIR_Gen_1	241	250	PF02991	0.417
LIG_LIR_Gen_1	388	397	PF02991	0.436
LIG_LIR_Gen_1	428	438	PF02991	0.426
LIG_LIR_LC3C_4	495	498	PF02991	0.487
LIG_LIR_Nem_3	160	166	PF02991	0.329
LIG_LIR_Nem_3	172	177	PF02991	0.356
LIG_LIR_Nem_3	309	313	PF02991	0.428
LIG_LIR_Nem_3	388	394	PF02991	0.425
LIG_LIR_Nem_3	428	433	PF02991	0.365
LIG_LIR_Nem_3	518	522	PF02991	0.323
LIG_Pex14_1	471	475	PF04695	0.373
LIG_Pex14_2	197	201	PF04695	0.302
LIG_PROFILIN_1	21	27	PF00235	0.511
LIG_REV1ctd_RIR_1	194	202	PF16727	0.381
LIG_SH2_SRC	374	377	PF00017	0.376
LIG_SH2_STAP1	124	128	PF00017	0.289
LIG_SH2_STAP1	163	167	PF00017	0.420
LIG_SH2_STAT5	124	127	PF00017	0.318
LIG_SH2_STAT5	166	169	PF00017	0.318
LIG_SH2_STAT5	206	209	PF00017	0.365
LIG_SH2_STAT5	374	377	PF00017	0.342
LIG_SH2_STAT5	411	414	PF00017	0.417
LIG_SH2_STAT5	426	429	PF00017	0.399
LIG_SH3_1	18	24	PF00018	0.627
LIG_SH3_3	16	22	PF00018	0.656
LIG_SH3_3	23	29	PF00018	0.597
LIG_SH3_3	290	296	PF00018	0.382
LIG_SH3_3	336	342	PF00018	0.407
LIG_SH3_3	435	441	PF00018	0.372
LIG_SUMO_SIM_anti_2	214	219	PF11976	0.277
LIG_SUMO_SIM_anti_2	234	241	PF11976	0.322
LIG_SUMO_SIM_anti_2	322	330	PF11976	0.326
LIG_SUMO_SIM_anti_2	491	498	PF11976	0.433
LIG_SUMO_SIM_par_1	295	301	PF11976	0.423
LIG_TRAF2_1	253	256	PF00917	0.282
LIG_UBA3_1	340	348	PF00899	0.507
MOD_CDC14_SPxK_1	190	193	PF00782	0.544
MOD_CDK_SPxK_1	187	193	PF00069	0.551
MOD_CK1_1	122	128	PF00069	0.392
MOD_CK1_1	2	8	PF00069	0.748
MOD_CK1_1	228	234	PF00069	0.390
MOD_CK1_1	237	243	PF00069	0.347
MOD_CK1_1	383	389	PF00069	0.456
MOD_CK2_1	250	256	PF00069	0.282
MOD_CK2_1	485	491	PF00069	0.377
MOD_CK2_1	499	505	PF00069	0.384
MOD_Cter_Amidation	535	538	PF01082	0.531
MOD_Cter_Amidation	77	80	PF01082	0.628
MOD_GlcNHglycan	121	124	PF01048	0.399
MOD_GlcNHglycan	14	17	PF01048	0.777
MOD_GlcNHglycan	266	269	PF01048	0.492
MOD_GlcNHglycan	385	388	PF01048	0.452
MOD_GlcNHglycan	393	397	PF01048	0.465
MOD_GlcNHglycan	446	449	PF01048	0.668
MOD_GlcNHglycan	487	490	PF01048	0.491
MOD_GlcNHglycan	52	55	PF01048	0.682
MOD_GSK3_1	118	125	PF00069	0.309
MOD_GSK3_1	138	145	PF00069	0.468
MOD_GSK3_1	234	241	PF00069	0.370
MOD_GSK3_1	346	353	PF00069	0.433
MOD_GSK3_1	357	364	PF00069	0.456
MOD_GSK3_1	392	399	PF00069	0.406
MOD_N-GLC_1	369	374	PF02516	0.504
MOD_N-GLC_2	82	84	PF02516	0.651
MOD_NEK2_1	1	6	PF00069	0.602
MOD_NEK2_1	201	206	PF00069	0.304
MOD_NEK2_1	250	255	PF00069	0.414
MOD_NEK2_1	392	397	PF00069	0.417
MOD_NEK2_1	464	469	PF00069	0.330
MOD_NEK2_1	88	93	PF00069	0.394
MOD_NEK2_2	369	374	PF00069	0.401
MOD_PIKK_1	179	185	PF00454	0.492
MOD_PIKK_1	357	363	PF00454	0.374
MOD_PIKK_1	385	391	PF00454	0.468
MOD_PIKK_1	465	471	PF00454	0.524
MOD_PKA_2	441	447	PF00069	0.590
MOD_PKA_2	74	80	PF00069	0.595
MOD_PKB_1	461	469	PF00069	0.416
MOD_Plk_1	148	154	PF00069	0.504
MOD_Plk_1	159	165	PF00069	0.391
MOD_Plk_1	234	240	PF00069	0.318
MOD_Plk_1	346	352	PF00069	0.489
MOD_Plk_1	369	375	PF00069	0.470
MOD_Plk_1	476	482	PF00069	0.494
MOD_Plk_4	201	207	PF00069	0.366
MOD_Plk_4	234	240	PF00069	0.318
MOD_Plk_4	346	352	PF00069	0.466
MOD_Plk_4	515	521	PF00069	0.310
MOD_ProDKin_1	138	144	PF00069	0.329
MOD_ProDKin_1	17	23	PF00069	0.740
MOD_ProDKin_1	187	193	PF00069	0.566
MOD_SUMO_rev_2	322	327	PF00179	0.353
MOD_SUMO_rev_2	413	419	PF00179	0.497
MOD_SUMO_rev_2	449	458	PF00179	0.642
TRG_DiLeu_BaEn_2	104	110	PF01217	0.429
TRG_DiLeu_BaLyEn_6	153	158	PF01217	0.497
TRG_ENDOCYTIC_2	174	177	PF00928	0.449
TRG_ENDOCYTIC_2	244	247	PF00928	0.414
TRG_ENDOCYTIC_2	475	478	PF00928	0.400
TRG_ER_diArg_1	106	109	PF00400	0.404
TRG_ER_diArg_1	33	36	PF00400	0.605
TRG_ER_diArg_1	404	406	PF00400	0.364
TRG_ER_diArg_1	537	539	PF00400	0.553
TRG_ER_diArg_1	61	64	PF00400	0.598
TRG_ER_diArg_1	79	81	PF00400	0.666
TRG_NLS_MonoExtC_3	59	64	PF00514	0.570
TRG_NLS_MonoExtN_4	59	64	PF00514	0.459
TRG_Pf-PMV_PEXEL_1	156	160	PF00026	0.462

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6C4	Leptomonas seymouri	74%	82%
A0A0S4KJ93	Bodo saltans	49%	100%
A0A1X0NM42	Trypanosomatidae	56%	100%
A0A3R7RIQ8	Trypanosoma rangeli	52%	100%
A4HPC7	Leishmania braziliensis	85%	98%
A4ICG7	Leishmania infantum	100%	100%
A6H751	Bos taurus	31%	92%
A6ZP80	Saccharomyces cerevisiae (strain YJM789)	31%	99%
B3LJR0	Saccharomyces cerevisiae (strain RM11-1a)	31%	99%
B5VSC3	Saccharomyces cerevisiae (strain AWRI1631)	31%	99%
C8ZGZ3	Saccharomyces cerevisiae (strain Lalvin EC1118 / Prise de mousse)	31%	99%
D0A3I6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	46%	100%
E7KIA3	Saccharomyces cerevisiae (strain AWRI796)	31%	99%
E7KUJ4	Saccharomyces cerevisiae (strain Lalvin QA23)	31%	99%
E7NMM0	Saccharomyces cerevisiae (strain FostersO)	31%	99%
E7Q9C7	Saccharomyces cerevisiae (strain FostersB)	30%	99%
E7QKX4	Saccharomyces cerevisiae (strain Zymaflore VL3)	31%	99%
E9AT38	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	99%
F4J2K2	Arabidopsis thaliana	30%	86%
F4JYE9	Arabidopsis thaliana	27%	100%
F4K2A1	Arabidopsis thaliana	29%	95%
O13492	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	29%	100%
O74742	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	32%	100%
P48760	Mus musculus	32%	92%
Q05932	Homo sapiens	32%	92%
Q08645	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	31%	99%
Q09509	Caenorhabditis elegans	29%	100%
Q12676	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	23%	100%
Q4Q1G0	Leishmania major	95%	99%
Q54CY5	Dictyostelium discoideum	29%	86%
Q8W035	Arabidopsis thaliana	32%	100%
Q924L9	Cricetulus griseus	28%	92%
Q9UTD0	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	26%	100%
Q9Y893	Candida albicans	30%	100%
V5BKB0	Trypanosoma cruzi	53%	100%