tRNA (adenine(58)-N(1))-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA methyltransferase complex GCD14 subunit, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8IIA8_LEIDO

TriTrypDb:

LdBPK_282570.1 , LdCL_280030800 , LDHU3_28.3490

Length:

353

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0031515	tRNA (m1A) methyltransferase complex	6	12
GO:0032991	protein-containing complex	1	12
GO:0034708	methyltransferase complex	4	12
GO:0043527	tRNA methyltransferase complex	5	12
GO:1902494	catalytic complex	2	12
GO:1990234	transferase complex	3	12
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

A0A3Q8IIA8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IIA8

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006396	RNA processing	6	1
GO:0006399	tRNA metabolic process	7	1
GO:0006400	tRNA modification	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008033	tRNA processing	8	1
GO:0008152	metabolic process	1	1
GO:0009451	RNA modification	5	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0030488	tRNA methylation	5	1
GO:0032259	methylation	2	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0008168	methyltransferase activity	4	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0008175	tRNA methyltransferase activity	5	12
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	12
GO:0016426	tRNA (adenine) methyltransferase activity	6	12
GO:0016429	tRNA (adenine-N1-)-methyltransferase activity	7	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	197	201	PF00656	0.423
CLV_NRD_NRD_1	323	325	PF00675	0.370
CLV_PCSK_KEX2_1	292	294	PF00082	0.413
CLV_PCSK_KEX2_1	322	324	PF00082	0.363
CLV_PCSK_PC1ET2_1	292	294	PF00082	0.413
CLV_PCSK_SKI1_1	324	328	PF00082	0.527
DOC_MAPK_gen_1	322	333	PF00069	0.502
DOC_MAPK_MEF2A_6	28	36	PF00069	0.492
DOC_PP2B_LxvP_1	228	231	PF13499	0.312
DOC_PP4_FxxP_1	216	219	PF00568	0.339
DOC_PP4_FxxP_1	286	289	PF00568	0.388
DOC_USP7_MATH_1	219	223	PF00917	0.450
DOC_USP7_MATH_1	84	88	PF00917	0.520
DOC_USP7_UBL2_3	344	348	PF12436	0.683
DOC_WW_Pin1_4	251	256	PF00397	0.300
DOC_WW_Pin1_4	40	45	PF00397	0.364
LIG_14-3-3_CanoR_1	306	310	PF00244	0.442
LIG_14-3-3_CanoR_1	324	333	PF00244	0.405
LIG_FHA_1	10	16	PF00498	0.645
LIG_FHA_1	105	111	PF00498	0.317
LIG_FHA_1	139	145	PF00498	0.329
LIG_FHA_1	41	47	PF00498	0.394
LIG_FHA_1	98	104	PF00498	0.328
LIG_LIR_Apic_2	214	219	PF02991	0.340
LIG_LIR_Apic_2	284	289	PF02991	0.318
LIG_LIR_Gen_1	224	235	PF02991	0.289
LIG_LIR_Gen_1	315	325	PF02991	0.354
LIG_LIR_LC3C_4	31	36	PF02991	0.502
LIG_LIR_Nem_3	183	188	PF02991	0.339
LIG_LIR_Nem_3	224	230	PF02991	0.289
LIG_LIR_Nem_3	284	290	PF02991	0.369
LIG_LIR_Nem_3	315	320	PF02991	0.342
LIG_PDZ_Class_1	348	353	PF00595	0.725
LIG_Pex14_2	287	291	PF04695	0.474
LIG_SH2_STAP1	111	115	PF00017	0.303
LIG_SH2_STAP1	155	159	PF00017	0.318
LIG_SH2_STAT5	155	158	PF00017	0.318
LIG_SH2_STAT5	317	320	PF00017	0.337
LIG_SH2_STAT5	73	76	PF00017	0.536
LIG_SH3_3	227	233	PF00018	0.312
LIG_SUMO_SIM_anti_2	112	119	PF11976	0.302
LIG_SUMO_SIM_anti_2	31	37	PF11976	0.366
LIG_SUMO_SIM_anti_2	86	93	PF11976	0.340
LIG_WRC_WIRS_1	250	255	PF05994	0.318
LIG_WRC_WIRS_1	317	322	PF05994	0.313
MOD_CK1_1	308	314	PF00069	0.543
MOD_Cter_Amidation	126	129	PF01082	0.450
MOD_GlcNHglycan	136	139	PF01048	0.303
MOD_GlcNHglycan	150	153	PF01048	0.303
MOD_GlcNHglycan	314	317	PF01048	0.380
MOD_GlcNHglycan	94	97	PF01048	0.337
MOD_GSK3_1	132	139	PF00069	0.303
MOD_GSK3_1	308	315	PF00069	0.427
MOD_N-GLC_1	40	45	PF02516	0.418
MOD_N-GLC_2	239	241	PF02516	0.423
MOD_NEK2_1	140	145	PF00069	0.318
MOD_NEK2_1	180	185	PF00069	0.388
MOD_NEK2_1	249	254	PF00069	0.318
MOD_NEK2_1	257	262	PF00069	0.318
MOD_NEK2_1	3	8	PF00069	0.691
MOD_NEK2_1	305	310	PF00069	0.564
MOD_PIKK_1	104	110	PF00454	0.295
MOD_PIKK_1	180	186	PF00454	0.423
MOD_PIKK_1	257	263	PF00454	0.292
MOD_PIKK_1	97	103	PF00454	0.308
MOD_PK_1	85	91	PF00069	0.519
MOD_PKA_2	22	28	PF00069	0.400
MOD_PKA_2	294	300	PF00069	0.375
MOD_PKA_2	3	9	PF00069	0.656
MOD_PKA_2	305	311	PF00069	0.514
MOD_PKA_2	84	90	PF00069	0.545
MOD_PKB_1	298	306	PF00069	0.517
MOD_Plk_1	85	91	PF00069	0.344
MOD_Plk_2-3	276	282	PF00069	0.303
MOD_Plk_4	305	311	PF00069	0.497
MOD_Plk_4	61	67	PF00069	0.522
MOD_Plk_4	85	91	PF00069	0.445
MOD_ProDKin_1	251	257	PF00069	0.300
MOD_ProDKin_1	40	46	PF00069	0.365
MOD_SUMO_for_1	326	329	PF00179	0.534
TRG_DiLeu_BaEn_1	86	91	PF01217	0.547
TRG_ENDOCYTIC_2	317	320	PF00928	0.337
TRG_ER_diArg_1	322	324	PF00400	0.363

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PA18	Leptomonas seymouri	86%	100%
A0A0S4IUX6	Bodo saltans	57%	100%
A0A1X0NRB0	Trypanosomatidae	63%	100%
A0A3R7LPM7	Trypanosoma rangeli	64%	100%
A4I3X1	Leishmania infantum	100%	100%
A6H791	Bos taurus	36%	100%
D0A938	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	60%	84%
E9AIT3	Leishmania braziliensis	89%	100%
E9B061	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
O14307	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	34%	97%
P0CS08	Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565)	36%	82%
P0CS09	Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)	36%	82%
P46959	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	35%	92%
P9WFZ0	Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)	29%	100%
P9WFZ1	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	29%	100%
Q2U3W4	Aspergillus oryzae (strain ATCC 42149 / RIB 40)	31%	74%
Q4Q824	Leishmania major	93%	98%
Q4WUT7	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	29%	70%
Q5A416	Candida albicans (strain SC5314 / ATCC MYA-2876)	34%	100%
Q6AY46	Rattus norvegicus	36%	100%
Q6BX32	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	32%	96%
Q6C0P9	Yarrowia lipolytica (strain CLIB 122 / E 150)	33%	91%
Q6CN53	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	34%	87%
Q6FL77	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	33%	94%
Q755M8	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	34%	87%
Q80XC2	Mus musculus	36%	100%
Q86JJ0	Dictyostelium discoideum	32%	100%
Q96FX7	Homo sapiens	36%	100%
Q9V1J7	Pyrococcus abyssi (strain GE5 / Orsay)	26%	100%
V5BC39	Trypanosoma cruzi	63%	100%