RNA polymerase I

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

RNA polymerase I

Gene product:

kinetoplast poly(A) polymerase 1

Species:

Leishmania donovani

UniProt:

A0A3Q8IHZ8_LEIDO

TriTrypDb:

LdBPK_280840.1 , LdCL_280013000 , LDHU3_28.1010

Length:

455

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 22
NetGPI	no	yes: 0, no: 22

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	21
GO:0110165	cellular anatomical entity	1	22
GO:0016020	membrane	2	3
GO:0005739	mitochondrion	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0031019	mitochondrial mRNA editing complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0045293	mRNA editing complex	3	1
GO:0098798	mitochondrial protein-containing complex	2	1
GO:1902494	catalytic complex	2	1

Expansion

Sequence features

A0A3Q8IHZ8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IHZ8

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	23
GO:0006396	RNA processing	6	23
GO:0006725	cellular aromatic compound metabolic process	3	23
GO:0006807	nitrogen compound metabolic process	2	23
GO:0008152	metabolic process	1	23
GO:0009987	cellular process	1	23
GO:0016070	RNA metabolic process	5	23
GO:0031123	RNA 3'-end processing	7	23
GO:0034641	cellular nitrogen compound metabolic process	3	23
GO:0043170	macromolecule metabolic process	3	23
GO:0044237	cellular metabolic process	2	23
GO:0044238	primary metabolic process	2	23
GO:0046483	heterocycle metabolic process	3	23
GO:0071076	RNA 3' uridylation	8	23
GO:0071704	organic substance metabolic process	2	23
GO:0090304	nucleic acid metabolic process	4	23
GO:1901360	organic cyclic compound metabolic process	3	23
GO:0006378	mRNA polyadenylation	7	1
GO:0006397	mRNA processing	7	1
GO:0016071	mRNA metabolic process	6	1
GO:0031124	mRNA 3'-end processing	8	1
GO:0043631	RNA polyadenylation	6	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	23
GO:0004652	obsolete polynucleotide adenylyltransferase activity	6	5
GO:0005488	binding	1	23
GO:0016740	transferase activity	2	23
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	23
GO:0016779	nucleotidyltransferase activity	4	23
GO:0043167	ion binding	2	23
GO:0043169	cation binding	3	23
GO:0046872	metal ion binding	4	23
GO:0050265	RNA uridylyltransferase activity	4	23
GO:0070566	adenylyltransferase activity	5	5
GO:0070569	uridylyltransferase activity	5	23
GO:0140098	catalytic activity, acting on RNA	3	23
GO:0140640	catalytic activity, acting on a nucleic acid	2	23

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	378	380	PF00675	0.450
CLV_NRD_NRD_1	416	418	PF00675	0.494
CLV_NRD_NRD_1	45	47	PF00675	0.428
CLV_NRD_NRD_1	52	54	PF00675	0.372
CLV_PCSK_KEX2_1	418	420	PF00082	0.511
CLV_PCSK_KEX2_1	45	47	PF00082	0.408
CLV_PCSK_KEX2_1	52	54	PF00082	0.380
CLV_PCSK_PC1ET2_1	418	420	PF00082	0.592
CLV_PCSK_SKI1_1	198	202	PF00082	0.343
CLV_PCSK_SKI1_1	406	410	PF00082	0.572
CLV_PCSK_SKI1_1	427	431	PF00082	0.634
CLV_PCSK_SKI1_1	443	447	PF00082	0.504
CLV_Separin_Metazoa	42	46	PF03568	0.514
DEG_APCC_DBOX_1	197	205	PF00400	0.327
DEG_APCC_DBOX_1	405	413	PF00400	0.568
DEG_SPOP_SBC_1	133	137	PF00917	0.481
DOC_CYCLIN_RxL_1	336	346	PF00134	0.488
DOC_CYCLIN_yCln2_LP_2	155	161	PF00134	0.405
DOC_MAPK_MEF2A_6	146	155	PF00069	0.372
DOC_MAPK_MEF2A_6	351	359	PF00069	0.530
DOC_PP1_RVXF_1	409	415	PF00149	0.568
DOC_PP2B_LxvP_1	155	158	PF13499	0.381
DOC_PP4_FxxP_1	364	367	PF00568	0.393
DOC_USP7_MATH_1	133	137	PF00917	0.421
DOC_USP7_MATH_1	82	86	PF00917	0.576
DOC_USP7_UBL2_3	238	242	PF12436	0.305
DOC_USP7_UBL2_3	376	380	PF12436	0.561
DOC_WW_Pin1_4	253	258	PF00397	0.476
DOC_WW_Pin1_4	78	83	PF00397	0.462
LIG_14-3-3_CanoR_1	383	389	PF00244	0.494
LIG_14-3-3_CanoR_1	391	399	PF00244	0.482
LIG_14-3-3_CanoR_1	417	422	PF00244	0.535
LIG_14-3-3_CanoR_1	45	54	PF00244	0.525
LIG_14-3-3_CanoR_1	73	79	PF00244	0.497
LIG_BIR_III_2	266	270	PF00653	0.361
LIG_BRCT_BRCA1_1	123	127	PF00533	0.487
LIG_deltaCOP1_diTrp_1	120	127	PF00928	0.399
LIG_eIF4E_1	8	14	PF01652	0.443
LIG_FHA_1	13	19	PF00498	0.473
LIG_FHA_1	133	139	PF00498	0.387
LIG_FHA_1	225	231	PF00498	0.364
LIG_FHA_1	344	350	PF00498	0.360
LIG_FHA_1	440	446	PF00498	0.596
LIG_FHA_2	115	121	PF00498	0.454
LIG_FHA_2	279	285	PF00498	0.278
LIG_FHA_2	435	441	PF00498	0.497
LIG_LIR_Apic_2	166	171	PF02991	0.376
LIG_LIR_Apic_2	362	367	PF02991	0.398
LIG_LIR_Gen_1	124	133	PF02991	0.445
LIG_LIR_Gen_1	81	91	PF02991	0.509
LIG_LIR_Nem_3	124	130	PF02991	0.435
LIG_LIR_Nem_3	24	30	PF02991	0.343
LIG_LIR_Nem_3	281	285	PF02991	0.316
LIG_LIR_Nem_3	81	86	PF02991	0.497
LIG_Pex14_1	121	125	PF04695	0.519
LIG_Pex14_2	170	174	PF04695	0.364
LIG_Pex14_2	282	286	PF04695	0.464
LIG_PTB_Apo_2	255	262	PF02174	0.578
LIG_PTB_Phospho_1	255	261	PF10480	0.582
LIG_SH2_CRK	168	172	PF00017	0.352
LIG_SH2_STAP1	224	228	PF00017	0.313
LIG_SH2_STAP1	3	7	PF00017	0.393
LIG_SH2_STAT5	277	280	PF00017	0.393
LIG_SH2_STAT5	285	288	PF00017	0.393
LIG_SH2_STAT5	348	351	PF00017	0.406
LIG_SH2_STAT5	8	11	PF00017	0.362
LIG_SH3_1	76	82	PF00018	0.427
LIG_SH3_2	378	383	PF14604	0.441
LIG_SH3_3	148	154	PF00018	0.360
LIG_SH3_3	375	381	PF00018	0.409
LIG_SH3_3	76	82	PF00018	0.461
LIG_SUMO_SIM_anti_2	227	232	PF11976	0.382
LIG_SUMO_SIM_par_1	29	34	PF11976	0.472
LIG_UBA3_1	138	146	PF00899	0.389
LIG_UBA3_1	200	206	PF00899	0.349
LIG_WRC_WIRS_1	279	284	PF05994	0.286
LIG_WW_3	380	384	PF00397	0.498
MOD_CK1_1	372	378	PF00069	0.485
MOD_CK1_1	436	442	PF00069	0.567
MOD_CK2_1	114	120	PF00069	0.472
MOD_CK2_1	278	284	PF00069	0.278
MOD_CK2_1	44	50	PF00069	0.550
MOD_GlcNHglycan	371	374	PF01048	0.350
MOD_GSK3_1	176	183	PF00069	0.452
MOD_GSK3_1	220	227	PF00069	0.328
MOD_GSK3_1	292	299	PF00069	0.407
MOD_GSK3_1	74	81	PF00069	0.468
MOD_N-GLC_1	114	119	PF02516	0.464
MOD_N-GLC_1	121	126	PF02516	0.417
MOD_N-GLC_1	300	305	PF02516	0.195
MOD_N-GLC_1	433	438	PF02516	0.655
MOD_NEK2_1	13	18	PF00069	0.409
MOD_NEK2_1	132	137	PF00069	0.368
MOD_NEK2_1	174	179	PF00069	0.367
MOD_NEK2_1	181	186	PF00069	0.375
MOD_NEK2_1	220	225	PF00069	0.349
MOD_NEK2_1	278	283	PF00069	0.286
MOD_NEK2_1	302	307	PF00069	0.328
MOD_NEK2_1	343	348	PF00069	0.507
MOD_NEK2_1	369	374	PF00069	0.509
MOD_NEK2_1	44	49	PF00069	0.545
MOD_NEK2_1	92	97	PF00069	0.352
MOD_NEK2_2	3	8	PF00069	0.441
MOD_PKA_1	417	423	PF00069	0.545
MOD_PKA_1	45	51	PF00069	0.516
MOD_PKA_2	181	187	PF00069	0.343
MOD_PKA_2	44	50	PF00069	0.382
MOD_PKA_2	72	78	PF00069	0.432
MOD_PKB_1	107	115	PF00069	0.377
MOD_Plk_1	114	120	PF00069	0.435
MOD_Plk_1	121	127	PF00069	0.418
MOD_Plk_1	3	9	PF00069	0.432
MOD_Plk_1	300	306	PF00069	0.388
MOD_Plk_1	343	349	PF00069	0.452
MOD_Plk_4	134	140	PF00069	0.488
MOD_Plk_4	224	230	PF00069	0.317
MOD_Plk_4	232	238	PF00069	0.346
MOD_Plk_4	278	284	PF00069	0.453
MOD_Plk_4	292	298	PF00069	0.379
MOD_Plk_4	3	9	PF00069	0.412
MOD_ProDKin_1	253	259	PF00069	0.475
MOD_ProDKin_1	78	84	PF00069	0.457
TRG_DiLeu_BaEn_1	274	279	PF01217	0.360
TRG_ENDOCYTIC_2	261	264	PF00928	0.417
TRG_ER_diArg_1	106	109	PF00400	0.486
TRG_ER_diArg_1	44	46	PF00400	0.418
TRG_ER_diArg_1	51	53	PF00400	0.373
TRG_ER_diArg_1	56	59	PF00400	0.305
TRG_NLS_MonoExtC_3	416	422	PF00514	0.642
TRG_Pf-PMV_PEXEL_1	262	266	PF00026	0.451
TRG_Pf-PMV_PEXEL_1	339	344	PF00026	0.410
TRG_Pf-PMV_PEXEL_1	386	390	PF00026	0.613

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P857	Leptomonas seymouri	74%	87%
A0A0N1IJQ4	Leptomonas seymouri	26%	90%
A0A0S4JJR1	Bodo saltans	21%	91%
A0A0S4JJT6	Bodo saltans	43%	78%
A0A1X0NSS0	Trypanosomatidae	23%	93%
A0A1X0NZU6	Trypanosomatidae	59%	87%
A0A3Q8IC11	Leishmania donovani	26%	100%
A0A3R7K3P5	Trypanosoma rangeli	56%	86%
A0A422NVH7	Trypanosoma rangeli	23%	93%
A4HEP5	Leishmania braziliensis	25%	100%
A4HGC0	Leishmania braziliensis	90%	100%
A4I1X5	Leishmania infantum	26%	100%
A4I3F3	Leishmania infantum	99%	100%
C9ZS45	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	24%	93%
D0A7Y7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	57%	86%
E9AY21	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	100%
E9AZP1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
Q4Q8J1	Leishmania major	95%	100%
Q4Q9F2	Leishmania major	25%	100%
V5BG78	Trypanosoma cruzi	55%	86%
V5BQ07	Trypanosoma cruzi	23%	94%