DNA topoisomerase I

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

DNA topoisomerase I

Gene product:

DNA topoisomerase IB, large subunit

Species:

Leishmania donovani

UniProt:

A0A3Q8IHS4_LEIDO

TriTrypDb:

LdBPK_343220.1 , LdCL_340041100 , LDHU3_34.5400

Length:

635

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	yes	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	12
GO:0005694	chromosome	5	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043228	non-membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0043232	intracellular non-membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12
GO:0005654	nucleoplasm	2	1
GO:0005730	nucleolus	5	1
GO:0016020	membrane	2	1

Expansion

Sequence features

A0A3Q8IHS4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IHS4

PDB structure(s): 2b9s_A

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006259	DNA metabolic process	4	12
GO:0006265	DNA topological change	5	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0006996	organelle organization	4	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016043	cellular component organization	3	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0046483	heterocycle metabolic process	3	12
GO:0051276	chromosome organization	5	12
GO:0071103	DNA conformation change	6	12
GO:0071704	organic substance metabolic process	2	12
GO:0071840	cellular component organization or biogenesis	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006260	DNA replication	5	1
GO:0006355	regulation of DNA-templated transcription	6	1
GO:0007059	chromosome segregation	2	1
GO:0009889	regulation of biosynthetic process	4	1
GO:0010468	regulation of gene expression	5	1
GO:0010556	regulation of macromolecule biosynthetic process	5	1
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	1
GO:0019222	regulation of metabolic process	3	1
GO:0031323	regulation of cellular metabolic process	4	1
GO:0031326	regulation of cellular biosynthetic process	5	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0051171	regulation of nitrogen compound metabolic process	4	1
GO:0051252	regulation of RNA metabolic process	5	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0065007	biological regulation	1	1
GO:0080090	regulation of primary metabolic process	4	1
GO:1903506	regulation of nucleic acid-templated transcription	7	1
GO:2001141	regulation of RNA biosynthetic process	6	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003677	DNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0003916	DNA topoisomerase activity	3	12
GO:0003917	DNA topoisomerase type I (single strand cut, ATP-independent) activity	4	12
GO:0005488	binding	1	12
GO:0016853	isomerase activity	2	12
GO:0097159	organic cyclic compound binding	2	12
GO:0140097	catalytic activity, acting on DNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	155	159	PF00656	0.408
CLV_C14_Caspase3-7	399	403	PF00656	0.384
CLV_MEL_PAP_1	311	317	PF00089	0.284
CLV_NRD_NRD_1	102	104	PF00675	0.285
CLV_NRD_NRD_1	121	123	PF00675	0.101
CLV_NRD_NRD_1	172	174	PF00675	0.379
CLV_NRD_NRD_1	273	275	PF00675	0.322
CLV_NRD_NRD_1	376	378	PF00675	0.315
CLV_NRD_NRD_1	44	46	PF00675	0.405
CLV_NRD_NRD_1	475	477	PF00675	0.337
CLV_PCSK_KEX2_1	102	104	PF00082	0.294
CLV_PCSK_KEX2_1	11	13	PF00082	0.698
CLV_PCSK_KEX2_1	115	117	PF00082	0.261
CLV_PCSK_KEX2_1	121	123	PF00082	0.101
CLV_PCSK_KEX2_1	172	174	PF00082	0.401
CLV_PCSK_KEX2_1	376	378	PF00082	0.276
CLV_PCSK_KEX2_1	44	46	PF00082	0.284
CLV_PCSK_KEX2_1	531	533	PF00082	0.558
CLV_PCSK_KEX2_1	558	560	PF00082	0.657
CLV_PCSK_KEX2_1	614	616	PF00082	0.646
CLV_PCSK_PC1ET2_1	11	13	PF00082	0.698
CLV_PCSK_PC1ET2_1	115	117	PF00082	0.319
CLV_PCSK_PC1ET2_1	531	533	PF00082	0.558
CLV_PCSK_PC1ET2_1	558	560	PF00082	0.657
CLV_PCSK_PC1ET2_1	614	616	PF00082	0.641
CLV_PCSK_SKI1_1	103	107	PF00082	0.284
CLV_PCSK_SKI1_1	110	114	PF00082	0.284
CLV_PCSK_SKI1_1	151	155	PF00082	0.319
CLV_PCSK_SKI1_1	274	278	PF00082	0.284
CLV_PCSK_SKI1_1	310	314	PF00082	0.280
CLV_PCSK_SKI1_1	420	424	PF00082	0.308
CLV_PCSK_SKI1_1	459	463	PF00082	0.405
CLV_PCSK_SKI1_1	499	503	PF00082	0.509
CLV_PCSK_SKI1_1	559	563	PF00082	0.609
CLV_PCSK_SKI1_1	565	569	PF00082	0.646
CLV_PCSK_SKI1_1	588	592	PF00082	0.690
CLV_PCSK_SKI1_1	72	76	PF00082	0.319
DEG_MDM2_SWIB_1	105	113	PF02201	0.284
DEG_Nend_UBRbox_1	1	4	PF02207	0.698
DEG_SCF_FBW7_2	76	81	PF00400	0.284
DEG_SPOP_SBC_1	256	260	PF00917	0.319
DOC_ANK_TNKS_1	179	186	PF00023	0.300
DOC_CYCLIN_RxL_1	115	129	PF00134	0.285
DOC_MAPK_gen_1	376	384	PF00069	0.433
DOC_MAPK_gen_1	459	468	PF00069	0.431
DOC_PP2B_LxvP_1	55	58	PF13499	0.369
DOC_USP7_MATH_1	257	261	PF00917	0.370
DOC_USP7_MATH_1	579	583	PF00917	0.698
DOC_USP7_MATH_1	599	603	PF00917	0.652
DOC_USP7_UBL2_3	147	151	PF12436	0.279
DOC_USP7_UBL2_3	275	279	PF12436	0.431
DOC_USP7_UBL2_3	455	459	PF12436	0.319
DOC_USP7_UBL2_3	558	562	PF12436	0.658
DOC_USP7_UBL2_3	564	568	PF12436	0.614
DOC_USP7_UBL2_3	572	576	PF12436	0.568
DOC_USP7_UBL2_3	577	581	PF12436	0.638
DOC_USP7_UBL2_3	584	588	PF12436	0.535
DOC_USP7_UBL2_3	7	11	PF12436	0.666
DOC_WW_Pin1_4	376	381	PF00397	0.341
DOC_WW_Pin1_4	74	79	PF00397	0.297
LIG_14-3-3_CanoR_1	284	288	PF00244	0.341
LIG_APCC_ABBA_1	65	70	PF00400	0.332
LIG_BRCT_BRCA1_1	457	461	PF00533	0.405
LIG_CaM_IQ_9	463	479	PF13499	0.431
LIG_EVH1_2	183	187	PF00568	0.300
LIG_FHA_1	207	213	PF00498	0.319
LIG_FHA_1	476	482	PF00498	0.326
LIG_FHA_1	608	614	PF00498	0.723
LIG_FHA_2	144	150	PF00498	0.284
LIG_FHA_2	468	474	PF00498	0.393
LIG_FHA_2	75	81	PF00498	0.284
LIG_HCF-1_HBM_1	92	95	PF13415	0.300
LIG_LIR_Gen_1	235	246	PF02991	0.305
LIG_LIR_Gen_1	366	374	PF02991	0.356
LIG_LIR_Gen_1	383	393	PF02991	0.184
LIG_LIR_Gen_1	43	54	PF02991	0.300
LIG_LIR_Gen_1	524	533	PF02991	0.456
LIG_LIR_Gen_1	85	95	PF02991	0.284
LIG_LIR_Nem_3	107	112	PF02991	0.286
LIG_LIR_Nem_3	127	131	PF02991	0.341
LIG_LIR_Nem_3	366	371	PF02991	0.331
LIG_LIR_Nem_3	383	388	PF02991	0.181
LIG_LIR_Nem_3	43	49	PF02991	0.297
LIG_LIR_Nem_3	524	528	PF02991	0.523
LIG_PDZ_Class_2	630	635	PF00595	0.653
LIG_Pex14_1	512	516	PF04695	0.438
LIG_Pex14_2	101	105	PF04695	0.284
LIG_RPA_C_Insects	553	568	PF08784	0.580
LIG_SH2_CRK	368	372	PF00017	0.341
LIG_SH2_GRB2like	357	360	PF00017	0.300
LIG_SH2_GRB2like	439	442	PF00017	0.284
LIG_SH2_PTP2	252	255	PF00017	0.284
LIG_SH2_SRC	60	63	PF00017	0.341
LIG_SH2_STAT5	166	169	PF00017	0.431
LIG_SH2_STAT5	252	255	PF00017	0.284
LIG_SH2_STAT5	306	309	PF00017	0.284
LIG_SH2_STAT5	439	442	PF00017	0.283
LIG_SH2_STAT5	474	477	PF00017	0.319
LIG_SH3_1	423	429	PF00018	0.277
LIG_SH3_3	178	184	PF00018	0.298
LIG_SH3_3	215	221	PF00018	0.288
LIG_SH3_3	423	429	PF00018	0.277
LIG_SH3_3	51	57	PF00018	0.288
LIG_SUMO_SIM_anti_2	360	366	PF11976	0.369
LIG_SUMO_SIM_anti_2	445	451	PF11976	0.319
LIG_SUMO_SIM_anti_2	478	483	PF11976	0.384
LIG_SUMO_SIM_par_1	477	483	PF11976	0.400
LIG_TRAF2_1	146	149	PF00917	0.275
LIG_TRAF2_1	493	496	PF00917	0.515
LIG_TRAF2_1	77	80	PF00917	0.272
LIG_TRAF2_2	390	395	PF00917	0.405
LIG_UBA3_1	195	200	PF00899	0.319
LIG_UBA3_1	465	471	PF00899	0.393
LIG_UBA3_1	86	91	PF00899	0.405
LIG_WRC_WIRS_1	397	402	PF05994	0.289
LIG_WW_1	57	60	PF00397	0.300
MOD_CK1_1	472	478	PF00069	0.305
MOD_CK1_1	515	521	PF00069	0.555
MOD_CK1_1	580	586	PF00069	0.658
MOD_CK2_1	105	111	PF00069	0.300
MOD_CK2_1	143	149	PF00069	0.284
MOD_CK2_1	283	289	PF00069	0.379
MOD_CK2_1	467	473	PF00069	0.443
MOD_CK2_1	490	496	PF00069	0.562
MOD_CK2_1	74	80	PF00069	0.284
MOD_Cter_Amidation	170	173	PF01082	0.284
MOD_Cter_Amidation	555	558	PF01082	0.660
MOD_GlcNHglycan	404	407	PF01048	0.304
MOD_GlcNHglycan	545	548	PF01048	0.667
MOD_GlcNHglycan	552	555	PF01048	0.556
MOD_GlcNHglycan	565	568	PF01048	0.540
MOD_GSK3_1	372	379	PF00069	0.405
MOD_GSK3_1	408	415	PF00069	0.284
MOD_LATS_1	453	459	PF00433	0.319
MOD_N-GLC_1	412	417	PF02516	0.282
MOD_NEK2_1	105	110	PF00069	0.300
MOD_NEK2_1	466	471	PF00069	0.415
MOD_NEK2_2	257	262	PF00069	0.300
MOD_NEK2_2	328	333	PF00069	0.284
MOD_NEK2_2	351	356	PF00069	0.300
MOD_PIKK_1	388	394	PF00454	0.405
MOD_PK_1	490	496	PF00069	0.562
MOD_PKA_1	141	147	PF00069	0.282
MOD_PKA_1	490	496	PF00069	0.499
MOD_PKA_1	541	547	PF00069	0.676
MOD_PKA_1	563	569	PF00069	0.671
MOD_PKA_2	242	248	PF00069	0.289
MOD_PKA_2	283	289	PF00069	0.300
MOD_PKA_2	372	378	PF00069	0.319
MOD_PKA_2	475	481	PF00069	0.369
MOD_Plk_1	206	212	PF00069	0.296
MOD_Plk_1	232	238	PF00069	0.290
MOD_Plk_1	412	418	PF00069	0.284
MOD_Plk_4	234	240	PF00069	0.284
MOD_Plk_4	283	289	PF00069	0.405
MOD_Plk_4	396	402	PF00069	0.304
MOD_Plk_4	82	88	PF00069	0.284
MOD_ProDKin_1	376	382	PF00069	0.341
MOD_ProDKin_1	74	80	PF00069	0.297
MOD_SUMO_for_1	1	4	PF00179	0.643
MOD_SUMO_for_1	10	13	PF00179	0.678
MOD_SUMO_for_1	268	271	PF00179	0.284
MOD_SUMO_for_1	40	43	PF00179	0.547
MOD_SUMO_for_1	590	593	PF00179	0.694
MOD_SUMO_rev_2	144	153	PF00179	0.288
MOD_SUMO_rev_2	286	293	PF00179	0.389
MOD_SUMO_rev_2	424	432	PF00179	0.369
MOD_SUMO_rev_2	539	544	PF00179	0.624
MOD_SUMO_rev_2	70	77	PF00179	0.300
TRG_DiLeu_BaEn_1	335	340	PF01217	0.405
TRG_DiLeu_BaEn_1	366	371	PF01217	0.405
TRG_ENDOCYTIC_2	252	255	PF00928	0.286
TRG_ENDOCYTIC_2	368	371	PF00928	0.300
TRG_ENDOCYTIC_2	95	98	PF00928	0.405
TRG_ER_diArg_1	101	103	PF00400	0.300
TRG_ER_diArg_1	120	122	PF00400	0.101
TRG_ER_diArg_1	187	190	PF00400	0.284
TRG_ER_diArg_1	44	46	PF00400	0.405
TRG_ER_diArg_1	535	538	PF00400	0.615
TRG_NLS_Bipartite_1	476	494	PF00514	0.557
TRG_NLS_Bipartite_1	541	561	PF00514	0.665
TRG_NLS_MonoCore_2	561	566	PF00514	0.715
TRG_NLS_MonoExtC_3	489	495	PF00514	0.545
TRG_NLS_MonoExtN_4	559	566	PF00514	0.630
TRG_Pf-PMV_PEXEL_1	110	114	PF00026	0.261
TRG_Pf-PMV_PEXEL_1	274	278	PF00026	0.369
TRG_Pf-PMV_PEXEL_1	588	592	PF00026	0.550

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0L0P4F8	Candida auris	51%	85%
A0A0N0P5Q3	Leptomonas seymouri	80%	98%
A0A0S4IPT9	Bodo saltans	55%	98%
A0A1X0PA36	Trypanosomatidae	75%	96%
A0A3R7MDP0	Trypanosoma rangeli	65%	95%
A4HB62	Leishmania braziliensis	89%	100%
A9Q1D5	Pan troglodytes	44%	100%
C9ZLY6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	61%	91%
E9B5D3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
O17966	Caenorhabditis elegans	50%	79%
P04786	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	44%	83%
P07799	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	53%	78%
P11387	Homo sapiens	52%	83%
P30181	Arabidopsis thaliana	52%	69%
P41512	Xenopus laevis	48%	77%
P93119	Daucus carota	49%	80%
Q00313	Candida albicans	49%	82%
Q04750	Mus musculus	52%	83%
Q07050	Cricetulus griseus	52%	83%
Q4Q2K0	Leishmania major	96%	100%
Q54RC3	Dictyostelium discoideum	49%	71%
Q6IM78	Rattus norvegicus	45%	100%
Q7YR26	Chlorocebus aethiops	51%	83%
Q8R4U6	Mus musculus	46%	100%
Q969P6	Homo sapiens	44%	100%
Q9FJ79	Arabidopsis thaliana	53%	69%
Q9GPZ9	Leishmania infantum	100%	100%
Q9WUL0	Rattus norvegicus	52%	83%
V5BQI4	Trypanosoma cruzi	64%	96%