Reticulon-like protein

Quick info Annotations Localization Abundance Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

reticulon domain protein, 22 kDa potentially aggravating protein (paple22)

Species:

Leishmania donovani

UniProt:

A0A3Q8IFS1_LEIDO

TriTrypDb:

LdBPK_302570.1 , LdCL_300031300 , LDHU3_30.3470

Length:

197

Annotations

LeishMANIAdb annotations

Similar to reticulons (structural ER membrane proteins) of multicellular animals.. The toplology is difficult to assess, likely owing to the fact that these proteins are curvature-inducing. Localization: ER (by homology)

Annotations by Jardim et al.

ER/Golgi Proteins, Reticulon-like PAPLE22

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005789	endoplasmic reticulum membrane	4	11
GO:0016020	membrane	2	11
GO:0031090	organelle membrane	3	11
GO:0110165	cellular anatomical entity	1	11
GO:0005783	endoplasmic reticulum	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1

Abundance

Amastigote (protein)


Source	Evidence on protein	Close homologs
Pescher et al. (upgregulation)	no	yes: 0

Promastigote and amastigote


Source	Evidence on protein	Close homologs
Lahav et al. - mRNA - Protein

Expansion

Sequence features

A0A3Q8IFS1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IFS1

Function

Biological processes
Term	Name	Level	Count
GO:0009605	response to external stimulus	2	10
GO:0009607	response to biotic stimulus	2	10
GO:0009617	response to bacterium	3	10
GO:0043207	response to external biotic stimulus	3	10
GO:0044419	biological process involved in interspecies interaction between organisms	1	10
GO:0050896	response to stimulus	1	10
GO:0051707	response to other organism	2	10

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	193	195	PF00675	0.499
CLV_NRD_NRD_1	20	22	PF00675	0.222
CLV_PCSK_KEX2_1	20	22	PF00082	0.222
CLV_PCSK_KEX2_1	68	70	PF00082	0.360
CLV_PCSK_PC1ET2_1	68	70	PF00082	0.360
CLV_PCSK_SKI1_1	6	10	PF00082	0.428
DEG_MDM2_SWIB_1	32	40	PF02201	0.317
DOC_PP4_FxxP_1	143	146	PF00568	0.296
DOC_USP7_MATH_1	22	26	PF00917	0.520
DOC_USP7_MATH_1	85	89	PF00917	0.554
DOC_WW_Pin1_4	71	76	PF00397	0.527
LIG_14-3-3_CanoR_1	69	75	PF00244	0.508
LIG_APCC_ABBA_1	38	43	PF00400	0.361
LIG_BIR_II_1	1	5	PF00653	0.640
LIG_BRCT_BRCA1_1	111	115	PF00533	0.409
LIG_BRCT_BRCA1_1	29	33	PF00533	0.312
LIG_EH1_1	114	122	PF00400	0.409
LIG_FHA_1	11	17	PF00498	0.552
LIG_FHA_1	116	122	PF00498	0.352
LIG_FHA_1	131	137	PF00498	0.281
LIG_FHA_1	142	148	PF00498	0.259
LIG_FHA_1	45	51	PF00498	0.407
LIG_FHA_1	92	98	PF00498	0.547
LIG_FHA_2	10	16	PF00498	0.603
LIG_FHA_2	103	109	PF00498	0.504
LIG_FHA_2	174	180	PF00498	0.578
LIG_FHA_2	71	77	PF00498	0.448
LIG_LIR_Apic_2	18	22	PF02991	0.542
LIG_LIR_Gen_1	34	44	PF02991	0.329
LIG_LIR_Gen_1	83	93	PF02991	0.556
LIG_LIR_Nem_3	105	109	PF02991	0.527
LIG_LIR_Nem_3	34	39	PF02991	0.359
LIG_LIR_Nem_3	7	11	PF02991	0.596
LIG_LIR_Nem_3	83	89	PF02991	0.520
LIG_MLH1_MIPbox_1	29	33	PF16413	0.295
LIG_Pex14_2	124	128	PF04695	0.385
LIG_Pex14_2	29	33	PF04695	0.372
LIG_SH2_STAP1	109	113	PF00017	0.610
LIG_SH2_STAP1	166	170	PF00017	0.549
LIG_SH2_STAT5	170	173	PF00017	0.613
LIG_SH2_STAT5	44	47	PF00017	0.287
LIG_SH3_3	69	75	PF00018	0.508
LIG_SUMO_SIM_anti_2	100	105	PF11976	0.576
LIG_SUMO_SIM_anti_2	130	138	PF11976	0.347
LIG_SUMO_SIM_anti_2	55	60	PF11976	0.361
LIG_SUMO_SIM_par_1	98	105	PF11976	0.553
LIG_WRC_WIRS_1	29	34	PF05994	0.439
LIG_WRC_WIRS_1	46	51	PF05994	0.255
MOD_CK1_1	102	108	PF00069	0.470
MOD_CK1_1	130	136	PF00069	0.317
MOD_CK1_1	180	186	PF00069	0.648
MOD_CK1_1	31	37	PF00069	0.449
MOD_CK2_1	173	179	PF00069	0.601
MOD_CK2_1	180	186	PF00069	0.580
MOD_CK2_1	70	76	PF00069	0.564
MOD_GlcNHglycan	1	4	PF01048	0.430
MOD_GlcNHglycan	111	114	PF01048	0.315
MOD_GlcNHglycan	24	27	PF01048	0.371
MOD_GSK3_1	173	180	PF00069	0.647
MOD_GSK3_1	27	34	PF00069	0.378
MOD_GSK3_1	44	51	PF00069	0.304
MOD_GSK3_1	67	74	PF00069	0.577
MOD_NEK2_1	115	120	PF00069	0.355
MOD_NEK2_1	129	134	PF00069	0.256
MOD_NEK2_1	173	178	PF00069	0.633
MOD_NEK2_1	27	32	PF00069	0.289
MOD_NEK2_2	85	90	PF00069	0.476
MOD_PK_1	181	187	PF00069	0.594
MOD_PK_1	52	58	PF00069	0.361
MOD_PKA_1	181	187	PF00069	0.625
MOD_Plk_1	130	136	PF00069	0.317
MOD_Plk_1	42	48	PF00069	0.382
MOD_Plk_1	97	103	PF00069	0.554
MOD_Plk_4	116	122	PF00069	0.308
MOD_Plk_4	130	136	PF00069	0.253
MOD_Plk_4	28	34	PF00069	0.310
MOD_Plk_4	45	51	PF00069	0.397
MOD_Plk_4	52	58	PF00069	0.380
MOD_ProDKin_1	71	77	PF00069	0.527
MOD_SUMO_rev_2	191	196	PF00179	0.722
MOD_SUMO_rev_2	2	11	PF00179	0.602
TRG_ENDOCYTIC_2	170	173	PF00928	0.638
TRG_ENDOCYTIC_2	86	89	PF00928	0.534
TRG_ER_diArg_1	19	21	PF00400	0.422

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P527	Leptomonas seymouri	57%	100%
A0A0S4JFE7	Bodo saltans	30%	100%
A0A1X0P1J2	Trypanosomatidae	37%	100%
A0A3R7L2P9	Trypanosoma rangeli	35%	100%
A4HII9	Leishmania braziliensis	78%	100%
C9ZR54	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	100%
E9B128	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
O82352	Arabidopsis thaliana	25%	77%
Q28D16	Xenopus tropicalis	22%	92%
Q4Q737	Leishmania major	93%	100%
Q5J6M8	Xenopus laevis	24%	92%
Q68EW1	Xenopus laevis	23%	92%
Q9NJS2	Leishmania infantum	100%	100%