EF-hand domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania donovani

UniProt:

A0A3Q8IFG2_LEIDO

TriTrypDb:

LdBPK_292050.1 , LdCL_290026400 , LDHU3_29.2920

Length:

605

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	7
GO:0110165	cellular anatomical entity	1	7
GO:0005813	centrosome	3	1
GO:0005815	microtubule organizing center	2	1
GO:0036064	ciliary basal body	3	1

Expansion

Sequence features

A0A3Q8IFG2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IFG2

Function

Biological processes
Term	Name	Level	Count
GO:0019220	regulation of phosphate metabolic process	6	7
GO:0019222	regulation of metabolic process	3	7
GO:0031323	regulation of cellular metabolic process	4	7
GO:0035303	regulation of dephosphorylation	7	7
GO:0050789	regulation of biological process	2	7
GO:0050794	regulation of cellular process	3	7
GO:0051174	regulation of phosphorus metabolic process	5	7
GO:0065007	biological regulation	1	7
GO:0000226	microtubule cytoskeleton organization	3	1
GO:0006996	organelle organization	4	1
GO:0007010	cytoskeleton organization	5	1
GO:0007017	microtubule-based process	2	1
GO:0009987	cellular process	1	1
GO:0016043	cellular component organization	3	1
GO:0030865	cortical cytoskeleton organization	6	1
GO:0071840	cellular component organization or biogenesis	2	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	7
GO:0005509	calcium ion binding	5	6
GO:0043167	ion binding	2	7
GO:0043169	cation binding	3	7
GO:0046872	metal ion binding	4	7

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	336	340	PF00656	0.538
CLV_NRD_NRD_1	172	174	PF00675	0.839
CLV_NRD_NRD_1	378	380	PF00675	0.437
CLV_NRD_NRD_1	493	495	PF00675	0.463
CLV_NRD_NRD_1	61	63	PF00675	0.722
CLV_PCSK_KEX2_1	172	174	PF00082	0.837
CLV_PCSK_KEX2_1	242	244	PF00082	0.735
CLV_PCSK_KEX2_1	378	380	PF00082	0.437
CLV_PCSK_KEX2_1	61	63	PF00082	0.618
CLV_PCSK_PC1ET2_1	242	244	PF00082	0.735
CLV_PCSK_SKI1_1	366	370	PF00082	0.562
CLV_PCSK_SKI1_1	378	382	PF00082	0.448
CLV_PCSK_SKI1_1	389	393	PF00082	0.493
CLV_PCSK_SKI1_1	432	436	PF00082	0.576
DEG_SPOP_SBC_1	4	8	PF00917	0.649
DOC_CKS1_1	526	531	PF01111	0.471
DOC_CYCLIN_RxL_1	375	385	PF00134	0.443
DOC_CYCLIN_yCln2_LP_2	195	201	PF00134	0.748
DOC_MAPK_MEF2A_6	292	299	PF00069	0.510
DOC_PP1_RVXF_1	376	383	PF00149	0.439
DOC_PP1_RVXF_1	493	500	PF00149	0.441
DOC_PP2B_LxvP_1	151	154	PF13499	0.661
DOC_PP2B_LxvP_1	195	198	PF13499	0.743
DOC_PP2B_LxvP_1	548	551	PF13499	0.496
DOC_USP7_MATH_1	224	228	PF00917	0.756
DOC_USP7_MATH_1	4	8	PF00917	0.683
DOC_USP7_MATH_1	470	474	PF00917	0.546
DOC_USP7_MATH_1	507	511	PF00917	0.485
DOC_USP7_UBL2_3	103	107	PF12436	0.531
DOC_WW_Pin1_4	186	191	PF00397	0.853
DOC_WW_Pin1_4	206	211	PF00397	0.784
DOC_WW_Pin1_4	249	254	PF00397	0.682
DOC_WW_Pin1_4	466	471	PF00397	0.485
DOC_WW_Pin1_4	525	530	PF00397	0.476
DOC_WW_Pin1_4	577	582	PF00397	0.474
LIG_14-3-3_CanoR_1	124	130	PF00244	0.535
LIG_14-3-3_CanoR_1	19	24	PF00244	0.676
LIG_14-3-3_CanoR_1	341	347	PF00244	0.500
LIG_14-3-3_CanoR_1	393	398	PF00244	0.470
LIG_14-3-3_CanoR_1	87	96	PF00244	0.525
LIG_Actin_RPEL_3	572	591	PF02755	0.454
LIG_BIR_II_1	1	5	PF00653	0.788
LIG_BIR_III_4	222	226	PF00653	0.732
LIG_BRCT_BRCA1_1	6	10	PF00533	0.540
LIG_BRCT_BRCA1_1	65	69	PF00533	0.633
LIG_CtBP_PxDLS_1	198	202	PF00389	0.710
LIG_deltaCOP1_diTrp_1	40	47	PF00928	0.692
LIG_eIF4E_1	430	436	PF01652	0.579
LIG_EVH1_1	151	155	PF00568	0.625
LIG_FHA_1	15	21	PF00498	0.640
LIG_FHA_1	207	213	PF00498	0.813
LIG_FHA_1	424	430	PF00498	0.457
LIG_FHA_1	439	445	PF00498	0.498
LIG_FHA_1	480	486	PF00498	0.582
LIG_FHA_1	49	55	PF00498	0.596
LIG_FHA_1	522	528	PF00498	0.486
LIG_FHA_1	83	89	PF00498	0.549
LIG_FHA_2	155	161	PF00498	0.706
LIG_FHA_2	35	41	PF00498	0.625
LIG_FHA_2	411	417	PF00498	0.580
LIG_FHA_2	456	462	PF00498	0.575
LIG_FHA_2	485	491	PF00498	0.537
LIG_FHA_2	571	577	PF00498	0.475
LIG_LIR_Gen_1	130	139	PF02991	0.518
LIG_LIR_Gen_1	233	241	PF02991	0.606
LIG_LIR_Gen_1	404	412	PF02991	0.480
LIG_LIR_Gen_1	559	567	PF02991	0.489
LIG_LIR_Nem_3	130	135	PF02991	0.525
LIG_LIR_Nem_3	233	237	PF02991	0.584
LIG_LIR_Nem_3	404	409	PF02991	0.471
LIG_LIR_Nem_3	490	496	PF02991	0.457
LIG_LIR_Nem_3	559	564	PF02991	0.466
LIG_LIR_Nem_3	595	599	PF02991	0.454
LIG_LIR_Nem_3	7	13	PF02991	0.530
LIG_LIR_Nem_3	99	105	PF02991	0.525
LIG_NRBOX	114	120	PF00104	0.513
LIG_NRP_CendR_1	602	605	PF00754	0.578
LIG_PALB2_WD40_1	416	424	PF16756	0.584
LIG_PCNA_yPIPBox_3	271	283	PF02747	0.735
LIG_PTB_Apo_2	278	285	PF02174	0.714
LIG_PTB_Apo_2	530	537	PF02174	0.462
LIG_PTB_Phospho_1	278	284	PF10480	0.713
LIG_REV1ctd_RIR_1	533	541	PF16727	0.458
LIG_SH2_GRB2like	599	602	PF00017	0.459
LIG_SH2_STAP1	132	136	PF00017	0.506
LIG_SH2_STAP1	147	151	PF00017	0.622
LIG_SH2_STAT3	147	150	PF00017	0.625
LIG_SH2_STAT3	244	247	PF00017	0.670
LIG_SH2_STAT5	284	287	PF00017	0.573
LIG_SH2_STAT5	350	353	PF00017	0.476
LIG_SH2_STAT5	372	375	PF00017	0.424
LIG_SH2_STAT5	479	482	PF00017	0.466
LIG_SH2_STAT5	513	516	PF00017	0.550
LIG_SH3_3	149	155	PF00018	0.621
LIG_SH3_3	191	197	PF00018	0.730
LIG_SH3_3	285	291	PF00018	0.542
LIG_SH3_3	523	529	PF00018	0.479
LIG_SH3_3	575	581	PF00018	0.477
LIG_SUMO_SIM_par_1	11	17	PF11976	0.639
LIG_SUMO_SIM_par_1	498	504	PF11976	0.433
LIG_TRAF2_1	24	27	PF00917	0.619
LIG_TRAF2_1	38	41	PF00917	0.557
LIG_WRC_WIRS_1	20	25	PF05994	0.638
LIG_WRC_WIRS_1	315	320	PF05994	0.474
LIG_WW_3	289	293	PF00397	0.504
MOD_CK1_1	188	194	PF00069	0.800
MOD_CK1_1	2	8	PF00069	0.699
MOD_CK1_1	206	212	PF00069	0.738
MOD_CK1_1	226	232	PF00069	0.616
MOD_CK1_1	255	261	PF00069	0.703
MOD_CK1_1	552	558	PF00069	0.458
MOD_CK1_1	595	601	PF00069	0.460
MOD_CK2_1	159	165	PF00069	0.721
MOD_CK2_1	168	174	PF00069	0.762
MOD_CK2_1	34	40	PF00069	0.587
MOD_CK2_1	340	346	PF00069	0.504
MOD_CK2_1	484	490	PF00069	0.542
MOD_CK2_1	570	576	PF00069	0.486
MOD_Cter_Amidation	174	177	PF01082	0.624
MOD_DYRK1A_RPxSP_1	249	253	PF00069	0.590
MOD_GlcNHglycan	119	122	PF01048	0.636
MOD_GlcNHglycan	181	185	PF01048	0.870
MOD_GlcNHglycan	205	208	PF01048	0.701
MOD_GlcNHglycan	226	229	PF01048	0.687
MOD_GlcNHglycan	254	257	PF01048	0.681
MOD_GlcNHglycan	399	402	PF01048	0.482
MOD_GlcNHglycan	65	68	PF01048	0.629
MOD_GSK3_1	186	193	PF00069	0.833
MOD_GSK3_1	2	9	PF00069	0.596
MOD_GSK3_1	226	233	PF00069	0.704
MOD_GSK3_1	255	262	PF00069	0.729
MOD_GSK3_1	393	400	PF00069	0.428
MOD_GSK3_1	466	473	PF00069	0.577
MOD_GSK3_1	521	528	PF00069	0.484
MOD_GSK3_1	566	573	PF00069	0.539
MOD_N-GLC_1	186	191	PF02516	0.737
MOD_NEK2_1	205	210	PF00069	0.701
MOD_NEK2_1	213	218	PF00069	0.642
MOD_NEK2_1	259	264	PF00069	0.670
MOD_NEK2_1	297	302	PF00069	0.516
MOD_NEK2_1	3	8	PF00069	0.652
MOD_NEK2_1	33	38	PF00069	0.610
MOD_NEK2_1	333	338	PF00069	0.518
MOD_NEK2_1	397	402	PF00069	0.465
MOD_NEK2_1	435	440	PF00069	0.602
MOD_NEK2_1	521	526	PF00069	0.514
MOD_NEK2_1	536	541	PF00069	0.496
MOD_NEK2_1	88	93	PF00069	0.504
MOD_NEK2_2	470	475	PF00069	0.543
MOD_PIKK_1	14	20	PF00454	0.633
MOD_PIKK_1	226	232	PF00454	0.650
MOD_PIKK_1	41	47	PF00454	0.650
MOD_PIKK_1	423	429	PF00454	0.444
MOD_PKA_2	123	129	PF00069	0.539
MOD_PKA_2	340	346	PF00069	0.504
MOD_PKA_2	438	444	PF00069	0.492
MOD_PKA_2	536	542	PF00069	0.458
MOD_PKA_2	552	558	PF00069	0.480
MOD_Plk_1	122	128	PF00069	0.569
MOD_Plk_1	83	89	PF00069	0.573
MOD_Plk_4	154	160	PF00069	0.646
MOD_Plk_4	320	326	PF00069	0.459
MOD_Plk_4	393	399	PF00069	0.476
MOD_Plk_4	455	461	PF00069	0.677
MOD_Plk_4	470	476	PF00069	0.390
MOD_Plk_4	481	487	PF00069	0.465
MOD_Plk_4	552	558	PF00069	0.480
MOD_Plk_4	566	572	PF00069	0.519
MOD_Plk_4	592	598	PF00069	0.445
MOD_Plk_4	65	71	PF00069	0.628
MOD_ProDKin_1	186	192	PF00069	0.853
MOD_ProDKin_1	206	212	PF00069	0.786
MOD_ProDKin_1	249	255	PF00069	0.682
MOD_ProDKin_1	466	472	PF00069	0.478
MOD_ProDKin_1	525	531	PF00069	0.472
MOD_ProDKin_1	577	583	PF00069	0.467
MOD_SUMO_for_1	325	328	PF00179	0.452
TRG_DiLeu_BaEn_1	481	486	PF01217	0.522
TRG_DiLeu_BaEn_1	559	564	PF01217	0.466
TRG_DiLeu_BaEn_1	84	89	PF01217	0.573
TRG_DiLeu_LyEn_5	84	89	PF01217	0.573
TRG_ENDOCYTIC_2	132	135	PF00928	0.512
TRG_ENDOCYTIC_2	315	318	PF00928	0.481
TRG_ENDOCYTIC_2	430	433	PF00928	0.464
TRG_ENDOCYTIC_2	493	496	PF00928	0.453
TRG_ER_diArg_1	378	380	PF00400	0.437
TRG_ER_diArg_1	75	78	PF00400	0.719
TRG_Pf-PMV_PEXEL_1	378	383	PF00026	0.445
TRG_Pf-PMV_PEXEL_1	56	60	PF00026	0.704

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PC66	Leptomonas seymouri	69%	96%
A4HHJ3	Leishmania braziliensis	86%	100%
A4I4Q1	Leishmania infantum	99%	100%
E9AE54	Leishmania major	95%	100%
E9ALN1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%