tRNA:m(4)X modification enzyme TRM13

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

CCCH zinc finger in TRM13 protein/U11-48K-like CHHC zinc finger/Methyltransferase TRM13, putative

Species:

Leishmania donovani

UniProt:

A0A3Q8IFC1_LEIDO

TriTrypDb:

LdBPK_310540.1 , LdCL_310010900 , LDHU3_31.0770

Length:

626

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

A0A3Q8IFC1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: A0A3Q8IFC1

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006396	RNA processing	6	1
GO:0006399	tRNA metabolic process	7	1
GO:0006400	tRNA modification	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008033	tRNA processing	8	1
GO:0008152	metabolic process	1	1
GO:0009451	RNA modification	5	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0030488	tRNA methylation	5	1
GO:0032259	methylation	2	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	12
GO:0008168	methyltransferase activity	4	12
GO:0008171	O-methyltransferase activity	5	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0008175	tRNA methyltransferase activity	5	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0062105	RNA 2'-O-methyltransferase activity	5	12
GO:0106050	tRNA 2'-O-methyltransferase activity	6	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	231	233	PF00675	0.367
CLV_NRD_NRD_1	40	42	PF00675	0.436
CLV_NRD_NRD_1	419	421	PF00675	0.247
CLV_NRD_NRD_1	49	51	PF00675	0.414
CLV_NRD_NRD_1	577	579	PF00675	0.216
CLV_NRD_NRD_1	581	583	PF00675	0.216
CLV_NRD_NRD_1	76	78	PF00675	0.490
CLV_NRD_NRD_1	99	101	PF00675	0.182
CLV_PCSK_KEX2_1	40	42	PF00082	0.436
CLV_PCSK_KEX2_1	419	421	PF00082	0.164
CLV_PCSK_KEX2_1	49	51	PF00082	0.414
CLV_PCSK_KEX2_1	577	579	PF00082	0.173
CLV_PCSK_KEX2_1	585	587	PF00082	0.155
CLV_PCSK_PC1ET2_1	585	587	PF00082	0.167
CLV_PCSK_SKI1_1	136	140	PF00082	0.499
CLV_PCSK_SKI1_1	171	175	PF00082	0.193
CLV_PCSK_SKI1_1	180	184	PF00082	0.309
CLV_PCSK_SKI1_1	378	382	PF00082	0.161
CLV_PCSK_SKI1_1	41	45	PF00082	0.436
CLV_PCSK_SKI1_1	447	451	PF00082	0.172
CLV_PCSK_SKI1_1	50	54	PF00082	0.413
CLV_PCSK_SKI1_1	611	615	PF00082	0.228
DEG_APCC_DBOX_1	406	414	PF00400	0.419
DEG_APCC_DBOX_1	446	454	PF00400	0.372
DEG_APCC_DBOX_1	49	57	PF00400	0.411
DEG_APCC_DBOX_1	523	531	PF00400	0.380
DEG_SCF_FBW7_1	389	395	PF00400	0.341
DEG_SCF_FBW7_2	128	135	PF00400	0.469
DEG_SPOP_SBC_1	30	34	PF00917	0.427
DOC_CKS1_1	386	391	PF01111	0.361
DOC_CKS1_1	58	63	PF01111	0.423
DOC_MAPK_DCC_7	49	58	PF00069	0.411
DOC_MAPK_DCC_7	535	545	PF00069	0.341
DOC_MAPK_gen_1	40	48	PF00069	0.429
DOC_MAPK_gen_1	425	433	PF00069	0.382
DOC_MAPK_gen_1	49	55	PF00069	0.421
DOC_MAPK_gen_1	57	65	PF00069	0.420
DOC_MAPK_gen_1	582	593	PF00069	0.378
DOC_MAPK_HePTP_8	46	58	PF00069	0.420
DOC_MAPK_MEF2A_6	40	48	PF00069	0.429
DOC_MAPK_MEF2A_6	407	415	PF00069	0.399
DOC_MAPK_MEF2A_6	49	58	PF00069	0.422
DOC_MAPK_MEF2A_6	586	595	PF00069	0.458
DOC_PP1_RVXF_1	590	596	PF00149	0.458
DOC_PP2B_LxvP_1	543	546	PF13499	0.392
DOC_PP4_FxxP_1	58	61	PF00568	0.433
DOC_USP7_MATH_1	126	130	PF00917	0.491
DOC_USP7_MATH_1	157	161	PF00917	0.468
DOC_USP7_MATH_1	271	275	PF00917	0.559
DOC_USP7_MATH_1	30	34	PF00917	0.437
DOC_USP7_MATH_1	392	396	PF00917	0.367
DOC_USP7_MATH_1	455	459	PF00917	0.513
DOC_USP7_MATH_1	467	471	PF00917	0.448
DOC_USP7_MATH_1	546	550	PF00917	0.483
DOC_USP7_MATH_1	559	563	PF00917	0.432
DOC_USP7_MATH_1	61	65	PF00917	0.441
DOC_WW_Pin1_4	124	129	PF00397	0.523
DOC_WW_Pin1_4	138	143	PF00397	0.597
DOC_WW_Pin1_4	16	21	PF00397	0.393
DOC_WW_Pin1_4	382	387	PF00397	0.372
DOC_WW_Pin1_4	388	393	PF00397	0.525
DOC_WW_Pin1_4	498	503	PF00397	0.467
DOC_WW_Pin1_4	536	541	PF00397	0.371
DOC_WW_Pin1_4	57	62	PF00397	0.423
LIG_14-3-3_CanoR_1	103	111	PF00244	0.395
LIG_14-3-3_CanoR_1	180	189	PF00244	0.420
LIG_14-3-3_CanoR_1	216	222	PF00244	0.308
LIG_14-3-3_CanoR_1	319	327	PF00244	0.562
LIG_14-3-3_CanoR_1	41	47	PF00244	0.423
LIG_14-3-3_CanoR_1	419	426	PF00244	0.433
LIG_14-3-3_CanoR_1	427	433	PF00244	0.400
LIG_14-3-3_CanoR_1	447	457	PF00244	0.513
LIG_Actin_WH2_2	166	182	PF00022	0.416
LIG_BRCT_BRCA1_1	18	22	PF00533	0.384
LIG_BRCT_BRCA1_1	9	13	PF00533	0.386
LIG_Clathr_ClatBox_1	410	414	PF01394	0.394
LIG_FHA_1	116	122	PF00498	0.396
LIG_FHA_1	181	187	PF00498	0.369
LIG_FHA_1	310	316	PF00498	0.471
LIG_FHA_1	32	38	PF00498	0.437
LIG_FHA_1	359	365	PF00498	0.461
LIG_FHA_1	382	388	PF00498	0.503
LIG_FHA_1	43	49	PF00498	0.416
LIG_FHA_1	608	614	PF00498	0.451
LIG_FHA_2	216	222	PF00498	0.330
LIG_FHA_2	549	555	PF00498	0.444
LIG_FHA_2	607	613	PF00498	0.416
LIG_HP1_1	409	413	PF01393	0.458
LIG_Integrin_RGD_1	149	151	PF01839	0.386
LIG_LIR_Gen_1	330	337	PF02991	0.264
LIG_LIR_Gen_1	477	486	PF02991	0.372
LIG_LIR_Gen_1	554	563	PF02991	0.364
LIG_LIR_LC3C_4	245	248	PF02991	0.253
LIG_LIR_Nem_3	10	16	PF02991	0.386
LIG_LIR_Nem_3	330	335	PF02991	0.264
LIG_LIR_Nem_3	477	482	PF02991	0.389
LIG_LIR_Nem_3	554	560	PF02991	0.422
LIG_LIR_Nem_3	90	96	PF02991	0.264
LIG_Pex14_1	396	400	PF04695	0.361
LIG_SH2_CRK	295	299	PF00017	0.210
LIG_SH2_STAP1	165	169	PF00017	0.364
LIG_SH2_STAP1	238	242	PF00017	0.365
LIG_SH2_STAT5	115	118	PF00017	0.371
LIG_SH2_STAT5	181	184	PF00017	0.350
LIG_SH2_STAT5	188	191	PF00017	0.278
LIG_SH2_STAT5	598	601	PF00017	0.456
LIG_SH2_STAT5	608	611	PF00017	0.489
LIG_SH2_STAT5	97	100	PF00017	0.373
LIG_SH3_3	151	157	PF00018	0.182
LIG_SH3_3	383	389	PF00018	0.510
LIG_SH3_3	48	54	PF00018	0.415
LIG_SH3_3	507	513	PF00018	0.437
LIG_SH3_3	527	533	PF00018	0.324
LIG_SH3_3	79	85	PF00018	0.635
LIG_SH3_CIN85_PxpxPR_1	469	474	PF14604	0.346
LIG_SUMO_SIM_anti_2	245	250	PF11976	0.400
LIG_SUMO_SIM_par_1	408	414	PF11976	0.372
LIG_SUMO_SIM_par_1	513	519	PF11976	0.447
LIG_SxIP_EBH_1	465	474	PF03271	0.341
LIG_TRAF2_1	282	285	PF00917	0.439
LIG_TRAF2_1	553	556	PF00917	0.442
MOD_CK1_1	127	133	PF00069	0.598
MOD_CK1_1	140	146	PF00069	0.484
MOD_CK1_1	160	166	PF00069	0.324
MOD_CK1_1	269	275	PF00069	0.485
MOD_CK1_1	385	391	PF00069	0.461
MOD_CK1_1	395	401	PF00069	0.492
MOD_CK1_1	496	502	PF00069	0.405
MOD_CK1_1	562	568	PF00069	0.442
MOD_CK2_1	164	170	PF00069	0.429
MOD_CK2_1	360	366	PF00069	0.468
MOD_CK2_1	373	379	PF00069	0.530
MOD_CK2_1	548	554	PF00069	0.372
MOD_CK2_1	77	83	PF00069	0.642
MOD_Cter_Amidation	580	583	PF01082	0.172
MOD_GlcNHglycan	203	206	PF01048	0.379
MOD_GlcNHglycan	221	225	PF01048	0.297
MOD_GlcNHglycan	259	262	PF01048	0.501
MOD_GlcNHglycan	277	280	PF01048	0.415
MOD_GlcNHglycan	342	345	PF01048	0.309
MOD_GlcNHglycan	397	400	PF01048	0.274
MOD_GlcNHglycan	428	431	PF01048	0.294
MOD_GlcNHglycan	457	460	PF01048	0.209
MOD_GlcNHglycan	469	472	PF01048	0.146
MOD_GSK3_1	124	131	PF00069	0.476
MOD_GSK3_1	136	143	PF00069	0.695
MOD_GSK3_1	14	21	PF00069	0.387
MOD_GSK3_1	160	167	PF00069	0.412
MOD_GSK3_1	211	218	PF00069	0.436
MOD_GSK3_1	265	272	PF00069	0.544
MOD_GSK3_1	358	365	PF00069	0.381
MOD_GSK3_1	369	376	PF00069	0.418
MOD_GSK3_1	378	385	PF00069	0.461
MOD_GSK3_1	387	394	PF00069	0.482
MOD_GSK3_1	498	505	PF00069	0.474
MOD_GSK3_1	546	553	PF00069	0.451
MOD_GSK3_1	57	64	PF00069	0.445
MOD_GSK3_1	607	614	PF00069	0.409
MOD_GSK3_1	92	99	PF00069	0.264
MOD_N-GLC_1	160	165	PF02516	0.164
MOD_N-GLC_2	617	619	PF02516	0.253
MOD_NEK2_1	201	206	PF00069	0.355
MOD_NEK2_1	31	36	PF00069	0.432
MOD_NEK2_1	44	49	PF00069	0.419
MOD_NEK2_1	493	498	PF00069	0.424
MOD_NEK2_1	503	508	PF00069	0.472
MOD_NEK2_1	9	14	PF00069	0.384
MOD_NEK2_2	271	276	PF00069	0.458
MOD_OFUCOSY	11	18	PF10250	0.381
MOD_OFUCOSY	490	497	PF10250	0.180
MOD_PIKK_1	211	217	PF00454	0.491
MOD_PIKK_1	266	272	PF00454	0.441
MOD_PIKK_1	31	37	PF00454	0.440
MOD_PIKK_1	373	379	PF00454	0.358
MOD_PIKK_1	503	509	PF00454	0.372
MOD_PKA_1	419	425	PF00069	0.361
MOD_PKA_1	77	83	PF00069	0.475
MOD_PKA_2	215	221	PF00069	0.331
MOD_PKA_2	275	281	PF00069	0.612
MOD_PKA_2	318	324	PF00069	0.331
MOD_PKA_2	419	425	PF00069	0.395
MOD_PKA_2	426	432	PF00069	0.369
MOD_PKB_1	57	65	PF00069	0.429
MOD_Plk_1	160	166	PF00069	0.364
MOD_Plk_4	110	116	PF00069	0.364
MOD_Plk_4	160	166	PF00069	0.364
MOD_Plk_4	18	24	PF00069	0.384
MOD_Plk_4	311	317	PF00069	0.394
MOD_Plk_4	92	98	PF00069	0.312
MOD_ProDKin_1	124	130	PF00069	0.530
MOD_ProDKin_1	138	144	PF00069	0.595
MOD_ProDKin_1	16	22	PF00069	0.396
MOD_ProDKin_1	382	388	PF00069	0.372
MOD_ProDKin_1	498	504	PF00069	0.467
MOD_ProDKin_1	536	542	PF00069	0.371
MOD_ProDKin_1	57	63	PF00069	0.425
TRG_DiLeu_BaEn_3	615	621	PF01217	0.369
TRG_DiLeu_BaEn_4	284	290	PF01217	0.418
TRG_DiLeu_BaLyEn_6	499	504	PF01217	0.361
TRG_ENDOCYTIC_2	295	298	PF00928	0.212
TRG_ENDOCYTIC_2	601	604	PF00928	0.458
TRG_ER_diArg_1	418	420	PF00400	0.364
TRG_ER_diArg_1	435	438	PF00400	0.366
TRG_ER_diArg_1	48	50	PF00400	0.434
TRG_NLS_MonoExtC_3	581	586	PF00514	0.372
TRG_Pf-PMV_PEXEL_1	300	304	PF00026	0.373
TRG_Pf-PMV_PEXEL_1	586	590	PF00026	0.251

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IMP0	Leptomonas seymouri	57%	100%
A0A0S4IU97	Bodo saltans	34%	100%
A0A1X0NKZ4	Trypanosomatidae	37%	100%
A0A3R7N0X9	Trypanosoma rangeli	41%	100%
A4HJ30	Leishmania braziliensis	77%	100%
A4I6K9	Leishmania infantum	99%	100%
A7TSF4	Vanderwaltozyma polyspora (strain ATCC 22028 / DSM 70294 / BCRC 21397 / CBS 2163 / NBRC 10782 / NRRL Y-8283 / UCD 57-17)	21%	100%
C9ZN61	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	100%
E9B1K2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
Q4Q6L1	Leishmania major	91%	100%
V5BII3	Trypanosoma cruzi	40%	100%